Correlation analysis of transcriptome, proteome and metabolome of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature

doi:10.3969/j.issn.1004-1524.2017.11.08

›› 2017, Vol. 29 ›› Issue (11): 1827-1834.DOI: 10.3969/j.issn.1004-1524.2017.11.08

• Horticultural Science • Previous Articles Next Articles

Correlation analysis of transcriptome, proteome and metabolome of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature

HONG Senrong, WU-XIA Junpeng, XU Wenhui, ZHAN Xuelin, XIE Ni’ni, JIANG Yan, WANG Jinhua, LING Fei, WU Lixia, WAN Lin

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2017-04-05 Online:2017-11-20 Published:2017-12-05

Abstract

Abstract: In order to explore the internal mechanism of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature, the correlation of transcriptome, proteome and metabolome was analyzed. The results showed that Log₂ of transcript ratio and protein ratio of D. bulbifera L. microtubers conserved in vitro at low temperature and room temperature all showed normal distribution, which were very similar and consistent with the requirements of real biological samples; Wayne plot analysis of differential proteins and differential transcripts showed that the 84 transcripts were different in both groups, indicating that most of the transcripts were differentially expressed in proteome data; Log₂ logarithm thermal analysis of differential proteins and differential transcripts showed that the difference in transcriptome was greater than that in proteome, most of their differential transcripts were consistent, few were different; Analysis of log₂ logarithmic scatter correlation density plot showed that ratio of differential proteins and transcripts was in the vicinity of 0, the lower left corner and upper right corner that indicating the up/down regulation relationship of transcripts and proteins was consistent were more compared with the upper left corner and lower right corner that indicating the up/down regulation relationship of transcripts and proteins was not consistent, which indicated that consistency degree of the trend of differential expression was higher. Correlation analysis results of transcriptome, proteome and metabolome showed that conservation in vitro of D. bulbifera L. microtubers at low temperature mainly involved the pathway, such as carbon metabolism, amino acid biosynthesis, glycolysis, starch and sucrose metabolism, and pyruvate metabolism etc., which could provide a theoretical basis for conservation in vitro at low temperature and low temperature breaking dormancy for D. bulbifera L. microtubers.

Key words: Dioscorea bulbifera L., microtuber, conservation in vitro at low-temperature, transcriptome, proteome, metabolome

CLC Number:

S632.1

HONG Senrong, WU-XIA Junpeng, XU Wenhui, ZHAN Xuelin, XIE Ni’ni, JIANG Yan, WANG Jinhua, LING Fei, WU Lixia, WAN Lin. Correlation analysis of transcriptome, proteome and metabolome of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature[J]. , 2017, 29(11): 1827-1834.

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Correlation analysis of transcriptome, proteome and metabolome of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature

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