Cloning and expression analysis of PacAMR1 gene in sweet cherry

doi:10.3969/j.issn.1004-1524.2018.04.12

›› 2018, Vol. 30 ›› Issue (4): 607-613.DOI: 10.3969/j.issn.1004-1524.2018.04.12

• Horticultural Science • Previous Articles Next Articles

Cloning and expression analysis of PacAMR1 gene in sweet cherry

LI Jingxia¹, XIA Hui^{1, 2}, LYU Xiulan^{1, 2}, WANG Jin^{1, 2}, LIANG Dong^{1, 2, *}

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-10-07 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: Ascorbic acid (AsA) biosynthesis in plants occurs through a complex network. Regulation about this network is largely uncharacterized. PacAMR1, a gene that regulates pathway on AsA biosynthesis was identified from sweet cherry by reverse transcription-polymerase chain reaction (RT-PCR). The results of homology analysis showed that PacAMR1 protein shares high sequence similarity with that of other plants. Real-time PCR analysis showed that the relative expression level of PacAMR1 increased gradually with the development of fruit, however the AsA content was higher in early phage of fruit development. As leaves aged, PacAMR1transcripts accumulated with a concomitant increase. In contrast, the AsA content was highest in young leaves and lowest in old leaves. These results indicated that PacAMR1 appears to play an important role in modulating AsA biosynthesis in sweet cherry by negative regulating.

Key words: ascorbic acid, AMR1 gene, regulation, sweet cherry

CLC Number:

S662.5

LI Jingxia, XIA Hui, LYU Xiulan, WANG Jin, LIANG Dong. Cloning and expression analysis of PacAMR1 gene in sweet cherry[J]. , 2018, 30(4): 607-613.

References

[1] FOYER C H, NOCTOR G.Ascorbate and glutathione: The heart of the redox hub[J]. Plant Physiology , 2011, 155(1):12-18.
[2] LAING WA, BULLEY S, WRIGHT M, et al. A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesis[J]. Proceedings of the National Academy of Sciences of the United States of America , 2004, 101(48):16976-16981.
[3] BULLEY S M, RASSAM M, HOSER D, et al. Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis[J]. Journal of Experimental Botany , 2009, 60(3):765-778.
[4] LI M, MA F, LIANG D, et al. Ascorbate biosynthesis during early fruit development is the main reason for its accumulation in kiwi[J]. PLoS One , 2010, 5(12): e14281.
[5] HUANG M, XU Q, DENG X X. L-Ascorbic acid metabolism during fruit development in an ascorbate-rich fruit crop chestnut rose ( Rosa roxburghii Tratt)[J]. Journal of Plant Physiology , 2014, 171(14):1205-1216.
[6] LI M, CHEN X, WANG P, et al. Ascorbic acid accumulation and expression of genes involved in its biosynthesis and recycling in developing apple fruit[J]. Journal of the American Society for Horticultural Science , 2011, 136(4):231-238.
[7] IOANNIDI E, KALAMAKI M S, ENGINEER C, et al. Expression profiling of ascorbic acid-related genes during tomato fruit development and ripening and in response to stress conditions[J]. Journal of Experimental Botany , 2009, 60(2):663-678.
[8] CRUZ-RUS E, BOTELLA M A, VALPUESTA V, et al. Analysis of genes involved in L-ascorbic acid biosynthesis during growth and ripening of grape berries[J]. Journal of Plant Physiology , 2010, 167(9):739-748.
[9] CRUZ-RUS E, AMAYA I, SÁNCHEZ-SEVILLA J F, et al. Regulation of L-ascorbic acid content in strawberry fruits[J]. Journal of Experimental Botany , 2011, 62(12):4191-4201.
[10] LINSTER C L, CLARKE S G. L-ascorbate biosynthesis in higher plants: the role of VTC2[J]. Trends in Plant Science , 2008, 13(11):567-573.
[11] ALÓS E, RODRIGO M J, ZACARÍAS L. Differential transcriptional regulation of L-ascorbic acid content in peel and pulp of citrus fruits during development and maturation[J]. Planta , 2014, 239(5): 1113-1128.
[12] YE J, HU T X, YANG C M, et al. Transcriptome profiling of tomato fruit development reveals transcription factors associated with ascorbic acid, carotenoid and flavonoid biosynthesis[J]. PLoS One , 2015, 10(7): e0130885.
[13] LIU F H, WANG L, GU L, et al. Higher transcription levels in ascorbic acid biosynthetic and recycling genes were associated with higher ascorbic acid accumulation in blueberry[J]. Food Chemistry , 2015, 188: 399-405.
[14] ZHANG C M, HUANG J, LI X G. Transcriptomic analysis reveals the metabolic mechanism of l-ascorbic acid in Ziziphus jujuba Mill.[J]. Frontiers in Plant Science , 2016, 7: 122.
[15] MOON J, PARRY G, ESTELLE M. The ubiquitin-proteasome pathway and plant development[J]. Plant Cell , 2004, 16(12): 3181-3195.
[16] NI W, XIE D, HOBBIE L, et al. Regulation of flower development in Arabidopsis by SCF complexes[J]. Plant Physiology , 2004, 134(4): 1574-1585.
[17] ZHANG W, LORENCE A, GRUSZEWSKI H A, et al. AMR1, an Arabidopsis gene that coordinately and negatively regulates the mannose/L-galactose ascorbic acid biosynthetic pathway[J]. Plant Physiology , 2009, 150(2):942-950.
[18] GASIC K, HERNANDEZ A, KORBAN S S. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction[J]. Plant Molecular Biology Reporter , 2004, 22(4):437-438.
[19] BAI C, SEN P, HOFMANN K, et al. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box[J]. Cell , 1996, 86(2): 263-274.
[20] XIAO W Y, JANG J C. F-box proteins in Arabidopsis[J]. Trends in Plant Science , 2000, 5(11):454-457.
[21] WOO H R, CHUNG K M, PARK J H, et al. ORE9, an F-box protein that regulates leaf senescence in Arabidopsis[J]. Plant Cell , 2001, 13(8): 1779-1790.
[22] LIANG D, ZHU T T, NI Z Y, et al. Ascorbic acid metabolism during sweet cherry ( Prunus avium ) fruit development[J]. PLoS One , 2017, 12(2): e0172818.

Cloning and expression analysis of PacAMR1 gene in sweet cherry

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZOU Wenxiong, WU Wei, GUAN Yajing, CAO Dongdong, BIAN Xiaobo, SHI Deyun, DING Liling. Research progress of regulation techniques of rice seed dormancy [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 369-379.
[2]	ZHENG Xusong, ZHONG Liequan, WANG Huifu, CHEN Fangjing, CHENG Liping, XU Qiqiang, LI Yang, ZHONG Xuhua, LYU Zhongxian. Demonstration on rice pests control by fertilizer regulation technique in different geographic rice growing areas of Zhejiang Province [J]. , 2020, 32(9): 1656-1664.
[3]	WANG Baojun, CHENG Wangda, CHEN Gui, SHEN Yaqiang, SHEN Meng, YUAN Ye, WANG Lei, ZHANG Hongmei. Effects of nitrogen fertilizer regulation on soil properties of paddy fields and rice yield with full amount returning of straw in Northern Zhejiang [J]. , 2020, 32(2): 183-190.
[4]	PAN Xiaoqing, WANG Xinglong, YANG Jie, SHAO Le, QIN Feng, LI Jian, ZHANG Xia, ZHAI Pin. Effects of different LED light colors on estrus synchronization of rabbits and their molecular regulation mechanism through retina-pineal gland pathway [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2128-2137.
[5]	PAN Xiaoqing, WANG Xinglong, YANG Jie, SHAO Le, QIN Feng, LI Jian, ZHANG Xia, ZHAI Pin. Effects of different LED light colors on estrus synchronization of rabbits and their molecular regulation mechanism through retina-pineal gland pathway [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2128-2137.
[6]	LIU Huijie, XU Heng, QIU Wenyi, LI Xiaofang, ZHANG Hua, ZHU Ying, LI Chunshou, WANG Liangchao. Roles of bZIP transcription factors in plant growth and development and abiotic stress response [J]. , 2019, 31(7): 1205-1214.
[7]	GE Yi, XU Shaohui, XU Yan. Review on influencing factors of rhizosphere microbiome assemblage [J]. , 2019, 31(12): 2120-2130.
[8]	DAI Lin, XU Yuanyuan, XIONG Bo, HUANG Shengjia, SUN Guochao, WANG Zhihui. Accumulation characteristics of ascorbic acid, glutathione and organic acids in Huangguogan fruit [J]. , 2018, 30(8): 1341-1348.
[9]	XUE Wanwan, GONG Ronggao, DING Jianlin, LI Keqiang, ZOU Jin, LI Rulong, PENG Honggui. Effect of gibberellin on fruit quality and anatomical structure of Hongdeng sweet cherry [J]. , 2018, 30(6): 978-984.
[10]	CHEN Xijing, YU Man, WANG Qiang, LI Hua, SU Yao, GAO Jia, LI Guo'an, LI Jianqiang, SHEN Alin. Problems and countermeasures of straw returning in paddy field system in Zhejiang Province [J]. , 2018, 30(10): 1765-1774.
[11]	GAO Fan, XIA Hui, WANG Xiu, WANG Jin, ZHOU Qiong, LYU Xiulan, LIANG Dong. Antioxidant capacity analysis in different sweet cherry varieties [J]. , 2017, 29(6): 926-932.
[12]	WU Meiyan. Effects of spraying organic and inorganic nitrogen on physiological characteristics of rice in dry cultivation [J]. , 2017, 29(3): 353-359.
[13]	LI Dongyue, YUAN Wenxia, ZHENG Chao, WANG Xuming, ZHOU Jie, YAN Chengqi, CHEN Jianping. Roles of bZIP transcription factors in phytohormone-mediated disease resistance and stress tolerance [J]. , 2017, 29(1): 168-175.
[14]	HUANG Jing， ZHU Zhiwei， CHEN Xiaoyu， YU Fuxian， PAN Jianzhi *. Cloning and vector construction of chicken ovalbumin gene regulatory sequences [J]. , 2016, 28(3): 412-.
[15]	ZHANG Tao, LIU He-he, LUO Jun, LI Liang, HUANG Hui-lan, HE Hua. Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels [J]. , 2016, 28(12): 1985-1991.