Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR

doi:10.3969/j.issn.1004-1524.2018.04.13

›› 2018, Vol. 30 ›› Issue (4): 614-621.DOI: 10.3969/j.issn.1004-1524.2018.04.13

• Horticultural Science • Previous Articles Next Articles

Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR

WANG Xiangdong¹, MA Yanzhi^{2, *}, KE Shaoying²

1. Tangshan Academy of Agricultural Science, Tangshan 063001, China;
2. Department of Life Sciences, Tangshan Normal University, Tangshan 063000, China

Received:2017-08-04 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: The aim of the study was to analyze the genetic diversity of 11 Schizonepeta tenuifolia Briq.using ISSR, which would investigate the genetic diversity of Schizonepeta tenuifolia Briq. germplasm resources in China. With 11 kinds of Schizonepeta tenuifolia Briq. seedlings as materials, genomic DNA was extracted using the CTAB method. An orthogonal design was used to optimize the ISSR-PCR amplification system. 100 ISSR primers were used in the ISSR amplification. At last it counted the genetic distance between the tested materials and finally the cluster analysis was done. The results showed that a suitable ISSR reaction system was constructed with 25 μL reaction system containing 50 ng DNA, 0.75 μmol·L^-1 primer and 1 U Taq DNA polymerase. There were 55 primers amplified from the 100 primers, 458 bands were amplified, 8.3 bands were amplified from each primer, of which 246 bands were polymorphic, and the polymorphic rate was 53.71%. The genetic distances of 11 Schizonepeta tenuifolia Briq. germplasm resources were between 0.2083-0.7091. When the genetic distance was 0.50, 11 kinds of materials could be divided into 2 categories. When the genetic distance was 0.40, 11 kinds of materials could be divided into 5 categories. In conclusion, the genetic diversity of germplasm resources of Schizonepeta tenuifolia Briq. was lower in China.

Key words: Schizonepeta tenuifolia Briq., germplasm resources, ISSR, genetic diversity

CLC Number:

Q943

WANG Xiangdong, MA Yanzhi, KE Shaoying. Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR[J]. , 2018, 30(4): 614-621.

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Analysis of germplasm resources in 11 varieties Schizonepeta tenuifolia Briq. by ISSR

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