›› 2018, Vol. 30 ›› Issue (8): 1369-1375.DOI: 10.3969/j.issn.1004-1524.2018.08.14

• Plant Protection • Previous Articles     Next Articles

Identification of blackleg pathogen on potato (Solanum tuberosum L.) in Qinghai

WANG Xin, CHENG Liang, WANG Yayi, GAO Xusheng, LI Songling*   

  1. Qinghai Academy of Agriculture and Forestry Sciences, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China
  • Received:2017-08-07 Online:2018-08-25 Published:2018-08-28

Abstract: The causal pathogen causing blackleg of Solanum tuberosum L. was observed in the plantation in Qinghai Province in recent years. Five strains isolaed from five counties in Qinghai were characterized by morphological, biochemical and physiological methods, and analyses of 16S rRNA sequence as well as Pa subspecies geneome. The results indicated that all of the five strains were pathogenic to potato and caused blackleg of potato, suggesting that Pectobacterium atrosepticum was the blackleg pathogens on potato in Qinghai. The pathogenicity tests of the bacterial strains showed significant difference among the species isolated from different regions in Qinghai. Three highly pathogenic strains came from Ledu, Huangzhong and Datong, two moderately pathogenic strains mainly came from Minhe and Huangyuan. Pectobacterium atrosepticum was the blackleg pathogen on potato (Solanum tuberosum L.) in Qinghai Province.

Key words: Solanum tuberosum L., Pectobacterium atrosepticum, phenotype identification, molecular identification, pathogenicity

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