Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology

doi:10.3969/j.issn.1004-1524.2018.10.03

›› 2018, Vol. 30 ›› Issue (10): 1630-1639.DOI: 10.3969/j.issn.1004-1524.2018.10.03

• Animal Science • Previous Articles Next Articles

Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology

ZHANG Lirong¹, YANG Haiming^{1, *}, GONG Daoqing¹, XIAO Xia¹, TANG Xuhua¹, WANG Zhiyue^{1, 2}

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China

Received:2017-12-04 Online:2018-10-25 Published:2018-11-02

Abstract

Abstract: The aim of this experiment was to investigate the effect of diets supplemented with Bifidobacterium lactis on cecal microorganism diversity of layer chicks. A total of 216 healthy 1-day-old Roman male chicks with similar body weight were selected and randomly divided into two groups with 6 replicates per group (18 chicks per replicate). The chicks in control group were fed with a basal diet (group C) and the chicks in treatment group were fed with the basal diet supplemented with 1×10⁷ cfu·g^-1 Bifidobacterium lactis (group T). The experiment lasted for 42 d. After the experiment, 6 chicks per group were randomly selected, then the cecal chyme of three healthy chicks with similar body weight among them was collected to analyze cecal microorganism diversity by Illumina MiSeq sequencing technology. The results showed that: 1) Bifidobacterium lactis increased the body weight, average daily weight gain and average daily intake significantly (P<0.05). 2) The dominant bacteria in the two groups were all Bacteroidetes, Firmicutes and Proteobacteria; the ratio of Firmicutes to Bacteroidetes (F∶B) of group T was higher than that of group C. 3) Bifidobacterium lactis promoted the growth of probiotics such as Barnesiella, Romboutsia, Butyricicoccus, Lactobacillus and inhibited Clostridium sensu stricto, Escherichia-Shigella significantly (P<0.05). 4) Bifidobacterium lactis increased the abundance of cecal microorganism. The Shannon index and Simpson index of group T increased significantly (P<0.05). In conclusion, adding Bifidobacterium lactis in diet may exert probiotics effect by changing the structure and content of cecal microorganisms.

Key words: Bifidobacterium lactis, production performance, 16S rDNA, layer chicks, caecum, microorganisms, diversity

CLC Number:

ZHANG Lirong, YANG Haiming, GONG Daoqing, XIAO Xia, TANG Xuhua, WANG Zhiyue. Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology[J]. , 2018, 30(10): 1630-1639.

References

[1] 吕玉丽,徐子伟,吴建良,等. 饲用益生素在畜禽健康养殖中的应用[J]. 浙江农业学报,2012,24(1):180-186.
LYU Y L, XU Z W, WU J L, et al.Application of feeding probiotics in animal healthy cultivation[J]. Acta Agriculturae Zhejiangensis,2012, 24(1):180-186.(in Chinese with English abstract)
[2] 徐鹏,董晓芳,佟建明.微生物饲料添加剂的主要功能及其研究进展[J].动物营养学报,2012,24(8):1397-1403.
XU P, DONG X F, TONG J M.Microbial feed additives: major functions and lts research advances[J]. Chinese Journal of Animal Nutrition, 2012, 24(8):1397-1403.(in Chinese with English abstract)
[3] 胡学智. 益生元-双歧杆菌生长促进因子[J]. 工业微生物,2005,35(2):50-60.
HU X Z.Prebiotics-promoting Bifidobacterium growth[J]. Industrial Microbiology, 2005, 35(2): 50-60. (in Chinese)
[4] MERRIFIELD C A, LEWIS M C, CLAUS S P, et al.Weaning diet induces sustained metabolic phenotype shift in the pig and influences host response to Bifidobacterium lactis NCC2818[J]. Gut, 2013, 62(6):842-851.
[5] 宋晓玲,杨绪彤,偲瀚文,等. 双歧杆菌细胞壁肽聚糖的分离及其对二种海产动物免疫活性的影响[J]. 水产学报,2005,29(03):350-355.
SONG X L, YANG X T, CAI H W, et al.Peptidoglycan isolation from Bifidobacterium thermophilum and its effect on immuno-enzymatic activity of Penaeus japonicus and Paralichthys olivaceus[J]. Chinese Aquaculture Journal, 2005, 29(3):350-355. (in Chinese with English abstract)
[6] 范金波,侯宇,周素珍,等. 双歧杆菌增强小鼠机体的免疫功能[J]. 微生物学报,2015,55(4):484-491.
FAN J B, HOU Y, ZHOU S Z, et al.Effect of Bifidobacterium on the immunity in BALB/c mice[J]. Acta Microbiologica Sinica, 2015, 55(4):484-491. (in Chinese with English abstract)
[7] 彭骞骞,王雪敏,张敏红,等. 持续偏热环境对肉鸡盲肠菌群多样性的影响[J]. 中国农业科学,2016,49(1):186-194.
PENG Q Q, WANG X M, ZHANG M H, et al.Effects of constant moderate temperatures on the diversity of the intestinal microbial flora of broilers[J]. Scientia Agricultura Sinica, 2016, 49(1):186-194. (in Chinese with English abstract)
[8] 李美君,方成堃,张凯,等. 饲粮中添加乳铁蛋白对早期断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响[J]. 动物营养学报,2012,24(1):111-116.
LI M J, FANG C K, ZHANG K, et al.Effects of Lactoferrin supplementation on growth performance,intestinal microflora and mucosal morphology of early weaned piglets[J]. Acta Zoonutrimenta Sinica, 2012, 24(1):111-116. (in Chinese with English abstract)
[9] 郁二蒙,张振男,夏耘,等. 摄食不同饵料的大口黑鲈肠道菌群分析[J].水产学报,2015, 39(1):118-126.
YU E M, ZHANG Z, XIA Y, et al.Effects of different diets on intestinal microflora of largemouth bass (micropterus salmoides)[J]. Journal of Fisheries of China, 2015, 39(1):118-126. (in Chinese with English abstract)
[10] CROSS D E, MCDEVITT R M, HILLMAN K, et al.The effect of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age[J]. British Poultry Science, 2007, 48(4):496.
[11] 王丽凤,张家超,马晨,等.鸡肠道微生物研究进展[J].动物营养学报,2013,25(3):494-502.
WANG L F, ZHANG J C, MA C, et al.Gut microbiota of chickens: a review[J]. Acta Zoonutrimenta Sinica, 2013, 25(3):494-502.(in Chinese with English abstract)
[12] 秦楠,栗东芳,杨瑞馥. 高通量测序技术及其在微生物学研究中的应用[J]. 微生物学报,2011,51(4):445-457.
QIN N, LI D F, YANG R F.Next-generation sequencing technologies and the application in microbiology-a review[J]. Acta Microbiologica Sinica, 2011, 51(4):445-457. (in Chinese with English abstract)
[13] 王兴春,杨致荣,王敏,等. 高通量测序技术及其应用[J]. 中国生物工程杂志,2012,32(1):109-114.
WANG X C, YANG Z R, WANG M, et al.High-throughput sequencing technology and lts application[J]. China Biotechnology, 2012, 32(1):109-114.(in Chinese with English abstract)
[14] 钟世顺,张振书,王继德,等. 纯化黏附素介导的双歧杆菌黏附肠上皮细胞的研究[J]. 解放军医学杂志,2003,28(10):907-908.
ZHONG S S, ZHANG Z S, WANG J D, et al.Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells mediated by purified adhesion[J]. Medical Journal of the Chinese people's Liberation Army, 2003, 28(10):907-908.(in Chinese with English abstract)
[15] 刘国荣,潘伟好,畅晓渊,等. 双歧杆菌的粘附特性及其对肠道致病菌的体外拮抗作用[J]. 微生物学通报,2009,36(5):716-721.
LIU G R, PAN W H, CHANG X Y, et al.In vitro adherence properties of Bifidobacterium strains and their antagonistic activity against enteropathogens[J]. Microbiology, 2009, 36(5):716-721. (in Chinese with English abstract)
[16] 张玲,杨彩梅,曹广添,等. Toll样受体与益生菌免疫[J]. 动物营养学报,2013,25(1):21-25.
ZHANG L, YANG C M, CAO G T, et al.Toll-Like receptors and immune function of probiotics[J]. Acta Zoonutrimenta Sinica, 2013, 25(1):21-25. (in Chinese with English abstract)
[17] LEBEER S, VANDERLEYDEN J, DE KEERSMAECKER S C D. Host interactions of probiotic bacterial surface molecules: comparison with commensals and pathogens[J]. Nature Reviews Microbiology, 2010, 8(3):171-184.
[18] 林奕岑,徐帅,倪学勤,等. 利用Illumina MiSeq测序平台分析肉鸡盲肠微生物多样性[J]. 中国农业大学学报,2016,21(12):65-73.
LIN Y C, XU S, NI X Q, et al.Diversity of the cecal microbiome of broiler chicken based on Illumina MiSeq sequencing platform[J]. Journal of China Agricultural University, 2016, 21(12):65-73. (in Chinese with English abstract)
[19] 陈荣,刘利林, 格明古丽·木哈台,等. 16S rDNA克隆文库法分析拜城油鸡盲肠细菌多样性研究[J]. 家畜生态学报,2017,38(2):18-21.
CHEN R, LIU L L, GEMINGULI-MUHATAI, et al.Study on baicheng soy sauce chicken caecum bacterial diversity through 16S rDNA clone library analysis[J]. Acta Ecologae Animalis Domastici, 2017, 38(2):18-21. (in Chinese with English abstract)
[20] QU A, BRULC J M, WILSON M K, et al.Comparative metagenomics reveals host specific metavirulomes and horizontal gene transfer elements in the chicken cecum microbiome[J]. PLoS One, 2008, 3(8):e2945.
[21] 朱宏斌,沈伟,王竞,等. 宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系[J]. 第三军医大学学报,2017,39(8):773-780.
ZHU H B, SHEN W, WANG J, et al.Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study[J]. Journal of Third Military Medical University, 2017, 39(8):773-780. (in Chinese with English abstract)
[22] ISMAIL N A, RAGAB S H, ABD ELBAKY A, et al.Frequency of firmicutes and bacteroidetes in gut microbiota in obese and normal weight egyptian children and adults[J]. Archives of Medical Science, 2011, 7(3):501-507.
[23] WILKINSON T J, COWAN A A, VALLIN H E, et al.Characterization of the microbiome along the gastrointestinal tract of growing turkeys[J]. Frontiers in Microbiology, 2017, 8: 1089.
[24] RAUTIO M, EEROLA E, VÄISÄNEN-TUNKELROTT M L, et al. Reclassification of Bacteroides putredinis(Weinberg et al.,1937) in a new genus Alistipes gen. nov., as Alistipes putredinis comb. nov., and description of Alistipes finegoldii sp. nov., from human sources[J]. Systematic and Applied Microbiology, 2003, 26(2):182-188.
[25] SAKAMOTO M, LAN P T N, BENNO Y. Barnesiella viscericola gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from chicken caecum[J].International journal of systematic and evolutionary microbiology, 2007, 57(2):342-346.
[26] 赵滢. 抗生素诱导小鼠菌群变化与宿主代谢组相关性的研究[D]. 武汉:华中科技大学,2013.
ZHAO Y.Antibiotics-induced mice microbial alteration and their relationship with host metabolic profiles [D].Wuhan: Huazhong University of Science and Technology, 2013. (in Chinese with English abstract)
[27] UBEDA C, BUCCI V, CABALLERO S, et al.Intestinal microbiota containing barnesiella species cures vancomycin-resistant Enterococcus faecium colonization[J]. Infection and Immunity, 2013, 81(3):965-973.
[28] DUNCAN S H, HOLD G L, HARMSEN H J M, et al. Growth requirements and fermentation products of Fusobacterium prausnitzii, and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov[J]. International Journal of Systematic and Evolutionary Microbiology, 2002, 52(6):2141-2146.
[29] 张明明. Faecalibacterium prausnitzii抑制白介素17表达和对大鼠结肠炎的预防作用[D]. 南京:南京大学,2012.
ZHANG M M.Faecalibacterium prausnitzii inhibits interleukin 17 expression to ameliorate colitis in rats [D]. Nanjing: Nanjing University, 2012.(in Chinese with English abstract)
[30] GERRITSEN J.The genus Romboutsia: genomic and functional characterization of novel bacteria dedicated to life in the intestinal tract [D]. Netherlands: Wageningen University, 2015.
[31] EECKHAUT V, MACHIELS K, PERRIER C, et al.Butyricicoccus pullicaecorum in inflammatory bowel disease[J]. Gut, 2013, 62(12):1745-1752.
[32] KABEERDOSS J, SANKARAN V, PUGAZHENDHI S, et al.Clostridium leptum group bacteria abundance and diversity in the fecal microbiota of patients with inflammatory bowel disease: a case-control study in India[J]. BMC Gastroenterology, 2013, 13(1):20.
[33] HONDA K, TANOUE T, NAGANO Y, et al.Microbiota's influence on immunity: innate immunity: resistance and disease-promoting principles[J]. Else Kröner-Fresenius Symposium, 2013, 4(2):43-47.
[34] 邢荣春,郑军. 肠道梭状芽孢杆菌与调节性T细胞的研究进展[J]. 广东医学,2013,34(22):3525-3527.
XING R C, ZHENG J.Advances research of clostridium and regulatory T cells in gut[J]. Guangdong Medical Journal, 2013, 34(22):3525-3527.(in Chinese)
[35] ANDERSEN S J, DE G V, KHOR W C, et al.A clostridium group Ⅳ species dominates and suppresses a mixed culture fermentation by tolerance to medium chain fatty acids products[J]. Frontiers in Bioengineering and Biotechnology, 2017, 5: 8.
[36] CHEN L, ZHANG H F, GAO L X, et al.Effect of graded levels of fiber from alfalfa meal on intestinal nutrient and energy flow, and hindgut fermentation in growing pigs[J]. Journal of Animal Science, 2013, 91(10):4757-4764.
[37] IYAYI E A, ADEOLA O.Quantification of short-chain fatty acids and energy production from hindgut fermentation in cannulated pigs fed graded levels of wheat bran[J]. Journal of Animal Science, 2015, 93(10):4781-4757.
[38] 赵梅,王睿瑞,徐佳,等. 重度肥胖患者肠道内机会性致病菌Escherichia coli微多样性分析[J]. 基因组学与应用生物学,2015,34(02):252-256.
ZHAO M, WANG R R, XU J, et al.Micro-diversity analysis of opportunistic pathogen Escherichia coli isolated from a severely obese human gut[J]. Genomics and Applied Biology, 2015, 34(2):252-256.(in Chinese with English abstract)
[39] 蔡玟,崔岸,黄琼,等. 摄入含嗜酸乳杆菌NCFM和乳双歧杆菌Bi-07的益生菌补充剂增强免疫功能的动物实验研究[J]. 中国微生态学杂志,2008,20(1):17-19.
CAI M, CUI A, HUANG Q, et al.Animal studies on enhancement of immune function by dietary probiotic supplementation of Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07[J]. Chinese Journal of Microecology, 2008, 20(1):17-19. (in Chinese with English abstract)
[40] SERGEANT M J, CONSTANTINIDOU C, COGAN T A, et al.Extensive microbial and functional diversity within the chicken cecal microbiome[J]. PLoS One, 2014, 9(3):e91941.
[41] 李永洙,李进,张宁波,等. 热应激环境下蛋鸡肠道微生物菌群多样性[J]. 生态学报,2015,35(5):1601-1609.
LI Y Z, LI J, ZHANG N B, et al.Diversity analysis of the intestinal microbial flora of laying hens under heat stress[J]. Acta Ecologica Sinica, 2015, 35(5):1601-1609. (in Chinese with English abstract)
[42] 左之才,刘兵,李莉,等. 早期断乳应激性腹泻对仔猪肠道形态结构与肠道菌群的影响[J]. 中国兽医科学,2012,42(1):64-68.
ZUO Z C, LIU B, LI L, et al.Effects of early weaning irritable diarrhea on intestinal morphology and intestinal flora of piglets[J]. Chinese Veterinary Science, 2012, 42(1):64-68.(in Chinese with English abstract)

Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Minmin, HUANG Ying, LI Bo, XU Yan, ZHANG Shuai, YAO Lingyun, WANG Zheng. Effect of biochar on wheat root-associated microbial community structures [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 516-525.
[2]	WANG Zhihui, PENG Hua, YANG Puxiang, JIANG Xinfeng, LI Wenjin, YUE Cuinan, LI Chen, LI Yansheng. Phenotypic variation and resource value evaluation of natural hybrid progenies of seventeen Huangjinju tea plants [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 298-307.
[3]	HONG Xia, ZHAO Yongbin, QU Weidong, CHEN Yinlong, QIU Liping, WANG Jiaoyang. Comparative analysis on genetic diversity of Colocasia esculenta germplasm in Zhejiang Province based on phenotype and simple sequence repeats markers [J]. , 2020, 32(9): 1544-1554.
[4]	NIU Suzhen, ZHAO Zhifei, SONG Qinfei, CHEN Zhengwu. Eco-environmental diversity of wild tea germplasms in Guizhou Province [J]. , 2020, 32(7): 1223-1232.
[5]	YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen, WEI Shenghua. Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence [J]. , 2020, 32(5): 810-815.
[6]	GUO Qinwei, ZHANG Ting, LIU Huiqin, ZHANG Xinhui, LI Chaosen, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker [J]. , 2020, 32(4): 616-623.
[7]	Yakup HASANJAN, Omar ABLIZ, Mardan ASKAR, Aliya SIDIK. Diversity characteristics of soil oribatid mites in 7 microenvironments in Xinjiang [J]. , 2020, 32(4): 696-704.
[8]	WANG Baojun, CHENG Wangda, CHEN Gui, SHEN Yaqiang, SHEN Meng, YUAN Ye, WANG Lei, ZHANG Hongmei. Effects of nitrogen fertilizer regulation on soil properties of paddy fields and rice yield with full amount returning of straw in Northern Zhejiang [J]. , 2020, 32(2): 183-190.
[9]	ZHAO Xingkai, SHI Haichun, YU Xuejie, YANG Shu, ZHAO Changyun, XIA Wei, KE Yongpei. Breeding potential analyses of 13 new inbred lines in maize [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2119-2127.
[10]	HU Qianwen, XU Yanhao, WANG Rong, ZHANG Wenying, HUA Wei, LYU Chao. Association analysis of four spike traits in barley [J]. , 2020, 32(11): 1941-1953.
[11]	WANG Sutian, MENG Fanming, HU Bin, XIN Haiyun, LI Baohong, DU Zongliang, LI Jianhao. Growth characteristics and cross utilization of Tibetan pig in subtropical area [J]. , 2020, 32(11): 1963-1969.
[12]	SHEN Lu, LI Qinchao, TAKASHI Tanaka, TATSUYA Inamura, IRBIS Chagan. Efficiency of soil solarization on control of Chinese cabbage clubroot [J]. , 2020, 32(1): 98-107.
[13]	XIE Wengang, LI Xiaosong, LI Wei, TANG Qian. Genetic diversity of “Small & Medium Leaf” tea resources in Sichuan Province based on phenotypic characteristics [J]. , 2019, 31(9): 1405-1415.
[14]	ZENG Xueqin, LIU Chenjian, YANG Xue, LI Xiaoran. Microbial community structure and diversity of mastitis cows by 16S rRNA high-throughput sequencing [J]. , 2019, 31(9): 1437-1445.
[15]	YIN Xiaoxiao, LI Yanfang, GU Jiang, LIAO Yan, XIE Yue, YANG Guangyou, GU Xiaobin. Genetic diversity analysis of Psoroptes ovis var. cuniculi by full-length of mitochondrial ATP6 gene in China [J]. , 2019, 31(8): 1231-1238.