Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology

doi:10.3969/j.issn.1004-1524.2018.10.03

›› 2018, Vol. 30 ›› Issue (10): 1630-1639.DOI: 10.3969/j.issn.1004-1524.2018.10.03

• Animal Science • Previous Articles Next Articles

Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology

ZHANG Lirong¹, YANG Haiming^{1, *}, GONG Daoqing¹, XIAO Xia¹, TANG Xuhua¹, WANG Zhiyue^{1, 2}

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China

Received:2017-12-04 Online:2018-10-25 Published:2018-11-02

Abstract

Abstract: The aim of this experiment was to investigate the effect of diets supplemented with Bifidobacterium lactis on cecal microorganism diversity of layer chicks. A total of 216 healthy 1-day-old Roman male chicks with similar body weight were selected and randomly divided into two groups with 6 replicates per group (18 chicks per replicate). The chicks in control group were fed with a basal diet (group C) and the chicks in treatment group were fed with the basal diet supplemented with 1×10⁷ cfu·g^-1 Bifidobacterium lactis (group T). The experiment lasted for 42 d. After the experiment, 6 chicks per group were randomly selected, then the cecal chyme of three healthy chicks with similar body weight among them was collected to analyze cecal microorganism diversity by Illumina MiSeq sequencing technology. The results showed that: 1) Bifidobacterium lactis increased the body weight, average daily weight gain and average daily intake significantly (P<0.05). 2) The dominant bacteria in the two groups were all Bacteroidetes, Firmicutes and Proteobacteria; the ratio of Firmicutes to Bacteroidetes (F∶B) of group T was higher than that of group C. 3) Bifidobacterium lactis promoted the growth of probiotics such as Barnesiella, Romboutsia, Butyricicoccus, Lactobacillus and inhibited Clostridium sensu stricto, Escherichia-Shigella significantly (P<0.05). 4) Bifidobacterium lactis increased the abundance of cecal microorganism. The Shannon index and Simpson index of group T increased significantly (P<0.05). In conclusion, adding Bifidobacterium lactis in diet may exert probiotics effect by changing the structure and content of cecal microorganisms.

Key words: Bifidobacterium lactis, production performance, 16S rDNA, layer chicks, caecum, microorganisms, diversity

CLC Number:

ZHANG Lirong, YANG Haiming, GONG Daoqing, XIAO Xia, TANG Xuhua, WANG Zhiyue. Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology[J]. , 2018, 30(10): 1630-1639.

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Effects of diets supplemented with Bifidobacterium lactis on cecum microorganism diversity of layer chicks analyzed by Illumina MiSeq sequencing technology

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