Genetic diversity analysis of Tetrastigma hemsleyanum Diels et Gilg germplasm resources by random amplified polymorphic DNA technique

doi:10.3969/j.issn.1004-1524.2018.11.06

›› 2018, Vol. 30 ›› Issue (11): 1839-1848.DOI: 10.3969/j.issn.1004-1524.2018.11.06

• Horticultural Science • Previous Articles Next Articles

Genetic diversity analysis of Tetrastigma hemsleyanum Diels et Gilg germplasm resources by random amplified polymorphic DNA technique

YIN Minghua¹, ZHAN Xuelin¹, XU Wenhui¹, XIE Nini¹, CAI Hong², CHEN Ronghua²

1. College of Life Sciences, Shangrao Normal University, Shangrao 334001, China;
2. Shangrao Red Sun Agricultural Development Co., Ltd., Shangrao 334700, China

Received:2017-12-04 Online:2018-11-25 Published:2018-12-21

Abstract

Abstract: Genetic diversity of 64 samples of Chinese Tetrastigma hemsleyanum Diels et Gilg germplasm resources was analyzed by random amplified polymorphic DNA (RAPD). 10 pairs of primers were screened from 40 primers for batch PCR experiments. The results showed as follows: The observed number of alleles (Na) in 64 samples was 1.666 7-2.000 0, effective number of alleles (Ne) in 64 samples was 1.247 9-1.701 3, Nei's gene diversity index in 64 samples was 0.167 0-0.398 4, Shannon diversity index (I) in 64 samples was 0.262 8-0.583 0, the average polymorphic loci was 7.5, the average the percentage of polymorphism was 93.145%; 64 samples of Tetrastigma hemsleyanum Diels et Gilg were divided into 11 groups at the similarity of 0.720 56, and they were divided into 4 categories at the similarity of 0.697 04. The result of cluster analysis was not consistent with the geographical distance of the provenance, which might be related to the natural factors such as the topography and climate of the germplasm bank. The results suggested that Tetrastigma hemsleyanum Diels et Gilg germplasm resources were abundant in genetic diversity, and had certain value of exploitation and utilization. The results could also provide scientific basis for reasonable protection of genetic resources and genetic improvement of Tetrastigma hemsleyanum Diels et Gilg.

Key words: Tetrastigma hemsleyanum Diels et Gilg, germplasm resource, genetic diversity, random amplified polymorphic DNA

CLC Number:

S567.23

YIN Minghua, ZHAN Xuelin, XU Wenhui, XIE Nini, CAI Hong, CHEN Ronghua. Genetic diversity analysis of Tetrastigma hemsleyanum Diels et Gilg germplasm resources by random amplified polymorphic DNA technique[J]. , 2018, 30(11): 1839-1848.

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Genetic diversity analysis of Tetrastigma hemsleyanum Diels et Gilg germplasm resources by random amplified polymorphic DNA technique

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