Establishment of ELISA for Fusarium oxysporum f. sp. melonis

doi:10.3969/j.issn.1004-1524.2018.12.13

›› 2018, Vol. 30 ›› Issue (12): 2081-2086.DOI: 10.3969/j.issn.1004-1524.2018.12.13

• Plant Protection • Previous Articles Next Articles

Establishment of ELISA for Fusarium oxysporum f. sp. melonis

YAN Han¹, YANG Ruixiu², GAI Xiaotong², LI Gang¹, ZHAO Zhiguo¹, GAO Zenggui^2,*

1. Inspection and Quarantine Technology Center, Inner Mongolia Entry-Exit Inspection Bureau, Hohhot 010020, China;
2. Institute of Plant Immunity, Shenyang Agricultural University, Shenyang 110866, China

Received:2018-03-28 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: Polyclonal antibody was prepared by the mycelium protein of Fusarium oxysporum f. sp. melonis as antigen, and indirect ELISA method was established. The field samples and soil samples were detected by qualitative and quantity analysis, which showed charasteristics of convenience, specificity and high sensitivity. The ELISA detection technology could be easily applied in the field and have important practical value.

Key words: Fusarium oxysporum f. sp. melonis, polyclonal antibody, ELISA

CLC Number:

S412

YAN Han, YANG Ruixiu, GAI Xiaotong, LI Gang, ZHAO Zhiguo, GAO Zenggui. Establishment of ELISA for Fusarium oxysporum f. sp. melonis[J]. , 2018, 30(12): 2081-2086.

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Establishment of ELISA for Fusarium oxysporum f. sp. melonis

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