Identification of isolated fungus from root rot of Polygonatum sibiricum and its antagonistic endophytic bacteria

doi:10.3969/j.issn.1004-1524.2018.12.14

›› 2018, Vol. 30 ›› Issue (12): 2087-2093.DOI: 10.3969/j.issn.1004-1524.2018.12.14

• Plant Protection • Previous Articles Next Articles

Identification of isolated fungus from root rot of Polygonatum sibiricum and its antagonistic endophytic bacteria

WU Yiting, YAO Chuanwei, DENG Boxia, ZHANG Xia, LI Shenghua, ZOU Juan^*, FU Ming

Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China

Received:2018-01-31 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: Polygonatum sibiricum is a medicinal and food plant in China. The strain HF2, isolated from root rot of Polygonatum sibiricum in Hongjiang City, was classified and identified by morphological characteristics, ribosomal DNA (rDNA) internal transcribed spacer (ITS), EF-1a sequence analysis and its phylogenetic relationships with other species of the genus Fusarium. Bacteria were screened from endophytic resources of P. sibiricum against the strain HF2, and were identified by the characteristics of morphology, 16S rRNA sequence analysis and physiology and biochemistry tests. The results showed the strain HF2 was identified as Fusarium oxysporum. Of 40 endophytic bacterial strains isolated, ten strains exhibited good inhibition activity against F. oxysporum in vitro, and the strain A8 showed the highest antifungal activity with inhibition zone of 13 mm. The strain A8 was identified as Bacillus subtilis.

Key words: Fusarium oxysporum, inhibitory effect, endophytic bacteria, identification

CLC Number:

S436.661.14

WU Yiting, YAO Chuanwei, DENG Boxia, ZHANG Xia, LI Shenghua, ZOU Juan, FU Ming. Identification of isolated fungus from root rot of Polygonatum sibiricum and its antagonistic endophytic bacteria[J]. , 2018, 30(12): 2087-2093.

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Identification of isolated fungus from root rot of Polygonatum sibiricum and its antagonistic endophytic bacteria

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