›› 2019, Vol. 31 ›› Issue (9): 1478-1484.DOI: 10.3969/j.issn.1004-1524.2019.09.11

• Horticultural Science • Previous Articles     Next Articles

Cloning and expression of three chalcone synthase-like genes from Paeonia delavayi

YUAN Xiaolong, LI Yunqin, WANG Yi*   

  1. Yunnan Academy of Forestry, Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the National Forestry and Grassland Administration, Kunming 650201, China
  • Received:2019-04-01 Online:2019-09-25 Published:2019-10-11

Abstract: Three CHS genes were isolated from the transcriptome of wild Paeonia delavayi with yellow flower, gene functions were predicted by using bioinformatics analysis, and gene expression levels in different developmental period of flowers were also analyzed. The results showed that the full-length cDNA of these three CHS genes were 1 173, 1 185, 1128 bp, respectively. They were named as PdCHS1 (GenBank ID was MK516264), PdCHS2 (GenBank ID was MK516265) and PdCHS3 (GenBank ID was MK516266), and encoded 390, 394 and 375 aa, respectively. The proteins encoded by these three CHS genes were non-secreted proteins and had no signal peptide, they all contained highly conserved catalytic active motif (G/A) FGPG. Phylogenic analysis indicated that these 3 proteins belonged to different branches. Expression level of PdCHS1 was high in the stamina and budding stage, expression level of PdCHS2 was high in the blossom falling stage and initial flowering stage, and expression level of PdCHS3 was high in the blossom falling stage and budding stage. These three CHS genes could participate in the different biosynthesis of secondary metabolites, PdCHS1 might relate to the naringenin biosynthesis, PdCHS2 might take part in the stibenoid biosynthesis, and PdCHS3 might involve in the polyketide biosynthesis.

Key words: Paeonia delavayi, chalcone synthase gene, bioinformatics, biosynthetic pathways of metabolism

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