Screening and utilization of ammonia-nitrogen-degrading microorganism

doi:10.3969/j.issn.1004-1524.2020.09.17

›› 2020, Vol. 32 ›› Issue (9): 1683-1691.DOI: 10.3969/j.issn.1004-1524.2020.09.17

• Environmental Science • Previous Articles Next Articles

Screening and utilization of ammonia-nitrogen-degrading microorganism

SUN Xiaojun¹, SHEN Qi², WU Yifei², YAO Xiaohong², LI Yuancheng², SUN Hong², WANG Xin², TANG Jiangwu², GE Xiangyang^1,*

1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2020-03-24 Online:2020-09-25 Published:2020-10-10

Abstract

Abstract: To obtain the ammonia-lowering agent for livestock manure, the bacterial strains with good ammonia-nitrogen-degrading capability were isolated from swine manure. Three strains with high ammonia-nitrogen degradation efficiency were identified with the olfactory threshold method combined with the comparisons of their ammonia-nitrogen degradation efficiencies, and were encoded as Z2, AOZ and Y, respectively. Based on the results of morphological observation, physiological and biochemical tests, and 16S rRNA sequence homology analysis, the strains Z2, AOZ and Y were identified as Bacillus flexus, Pseudomonas putida, and Lactobacillus casei, respectively. The experimental results showed that the ammonia-nitrogen degradation efficiency was higher when strains Z2 and Y were mixed, and the highest ammonia-nitrogen degradation efficiency reached 53.19%, suggesting that the microorganism complex could be practically applied to remove ammonium from swine manure.

Key words: pig manure, ammonia-nitrogen-degrading microorganism, screening, compounding

CLC Number:

S182
X172

SUN Xiaojun, SHEN Qi, WU Yifei, YAO Xiaohong, LI Yuancheng, SUN Hong, WANG Xin, TANG Jiangwu, GE Xiangyang. Screening and utilization of ammonia-nitrogen-degrading microorganism[J]. , 2020, 32(9): 1683-1691.

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Screening and utilization of ammonia-nitrogen-degrading microorganism

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