Abstract: In order to gain high-producing glucose oxidase bacteria from marine environment and provide new strains for glucose oxidase manufacture, producing glucose oxidase bacteria was separated with coloration culture method using methylene blue medium in Petri dish from sea mud, sea water and coral, the dominant producing glucose oxidase bacteria among them was screened with being coloration cultivated again, and it's producing glucose oxidase ability was measured with shaking liquid fermentation, and influence of pH and temperature on enzymatic activity were checked, afterwards it was identified in morphological, biochemical and 16S rDNA sequences. The results showed that several strains producing glucose oxidase were separated from sea mud, of which MS-4 was the highest producing glucose oxidase bacteria, it secreted glucose oxidase in fermentation fluid of normal LB culture reaching 8.8 U·mL^-1, and the glucose oxidase best fit act temperature was 14 ℃, pH was 7.0,and stability was maintained below 25 ℃. Synthesize identification showed that MS-4 was Bacillus cereus, the similarity of biochemical and 16S rDNA sequences were 98.8% and 100%, respectively. The experimental results indicated MS-4 was relatively high-producing glucose oxidase marine bacteria, and it could be studied and developed as a bacteria that produce glucose oxidase which could be used in food fresh keeping at low temperature.

Key words: marine bacteria, glucose oxidase, screening, identification