Identification and biocontrol activity analysis of biocontrol strain PF-1 based on genome-wide data

doi:10.3969/j.issn.1004-1524.2020.10.10

›› 2020, Vol. 32 ›› Issue (10): 1816-1822.DOI: 10.3969/j.issn.1004-1524.2020.10.10

• Plant Protection • Previous Articles Next Articles

Identification and biocontrol activity analysis of biocontrol strain PF-1 based on genome-wide data

QI Jiaming^1,2,3, SUN Shanshan^1,2,3, ZHANG Dongxu^1,4,*, XIAO Jianzhong⁴, XU Zhiwen^1,2,3

1. Leading Bio-Agricultural Co., Ltd., Qinhuangdao 066004, China;
2. Hebei Agricultural Biotechnology Innovation Center, Qinhuangdao 066004, China;
3. Key Laboratory of Plant Nutrition and Marine Functional Fertilizer, Agricultural and Rural Department of Hebei Province, Qinhuangdao 066004, China;
4. College of Ecology, Lishui University, Lishui 323000, China

Received:2020-01-03 Online:2020-10-25 Published:2020-11-16

Abstract

Abstract: Based on the genome-wide data, the taxonomic status of biocontrol strain PF-1, which was isolated from soil, was clarified. The antagonistic effect of biocontrol strain PF-1 on multiple plant pathogens was validated to uncover its potential biocontrol functions. Phylogenetic trees were constructed by 16S rRNA gene sequences analysis to identify biocontrol strain PF-1. Furthermore, whole-genome sequencing analysis was also employed to determine the taxonomic status of strain PF-1. Plate isolate-antagonistic experiments were applied to explore the antagonistic effect of strain PF-1 on Verticillium dahliae Kleb and Fusarium oxysporum. The antibiotic genes of strain PF-1 were figured out among secondary metabolite biosynthesis gene clusters predicted by antiSMASH. According to the results of phylogenetic trees, average nucleotide identity and digital DNA-DNA hybridization, PF-1 was characterized as Pseudomonas protegens, with 15 secondary metabolite gene clusters in genome, exhibited a good ability to inhibit the growth of plant pathogens. Pseudomonas protegens PF-1 has strong antagonistic ability against plant pathogens and good prospects for agricultural applications.

Key words: Pseudomonas protegens, antagonistic ability, genome, gene cluster

CLC Number:

S476

QI Jiaming, SUN Shanshan, ZHANG Dongxu, XIAO Jianzhong, XU Zhiwen. Identification and biocontrol activity analysis of biocontrol strain PF-1 based on genome-wide data[J]. , 2020, 32(10): 1816-1822.

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Identification and biocontrol activity analysis of biocontrol strain PF-1 based on genome-wide data

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