Genomic features of 3 bovine viral diarrhea viruses isolated from newborn claves

doi:10.3969/j.issn.1004-1524.20221234

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (8): 1814-1822.DOI: 10.3969/j.issn.1004-1524.20221234

• Animal Science • Previous Articles Next Articles

Genomic features of 3 bovine viral diarrhea viruses isolated from newborn claves

WANG Mengzhu¹^,²^,³(), YANG Guangmei³, WU Yuhu¹^,²^,³, YANG Xuanye¹^,²^,³, WANG Huihui¹^,²^,³, CAO Xiaoan⁴, LI Yong⁴, MA Zhongren¹^,², MA Xiaoxia¹^,²^,^*()

1. Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China
2. Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China
3. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730010, China
4. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:2022-08-22 Online:2023-08-25 Published:2023-08-29

Abstract

Abstract:

To study the genetic evolution of Bovine viral diarrhea virus(BVDV)-infected newborn calves in different regions of China, the newborn Holstein calf serum from Shandong, Inner Mongolia and Ningxia provinces preserved in the laboratory was used as the material, and the calf serum was detected by RT-PCR for BVDV RNA, and the positive serum was inoculated into Madin-Darby bovine kidney cells (MDBK). Three isolates were identified as BVDV and named as 21NM-44, 21NX-53 and 21SD-16, respectively. According to the cytopathic ability of the 3 isolates to MDBK cells, 21NM-44 was determined to be a non-cytopathic strain, while 21NX-53 and 21SD-16 could cause cytopathic and were determined to be cytopathic strains. Using specific PCR primers, the whole genome sequences of the 3 viruses were cloned and identified. Based on the whole genome sequences, a phylogenetic tree was established and their genetic evolution relationship was analyzed. The results showed that the 3 wild BVDV isolates from different regions had a high degree of genetic evolution relationship with the BVDV GXNN1 strain (BVDV-1c). This study made a foundation for further research on the evolution of BVDV in China.

Key words: Bovine viral diarrhea virus, newborn calf, whole genome, Madin-Darby bovine kidney cell, cytopathic ability

CLC Number:

S852.653

WANG Mengzhu, YANG Guangmei, WU Yuhu, YANG Xuanye, WANG Huihui, CAO Xiaoan, LI Yong, MA Zhongren, MA Xiaoxia. Genomic features of 3 bovine viral diarrhea viruses isolated from newborn claves[J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1814-1822.

Figures/Tables 7

References 30

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引物名称 Primer name	正向引物 Forward primer sequence (5'→3')	反向引物 Reverse primer sequence (5'→3')	产物长度 Product length/bp
BVDV1	TGAATCCCCTTCAGCGAAGGC	CATGTGCCATGTACAGCAGAG	319
BVDV2	CTAAAAATCTCTGCTGTACATG	CTGGTGGCTTGTTCTTGTTGTG	759
BVDV3	GAACAAGCCACCAGAGTCACGC	GTTCTTTAAACAGCACATCAC	596
BVDV4	CGATAGTAGTGGATGGGGTCAAG	TGTTACAAGAAGTGTCATCTAC	2 184
BVDV5	CGTCGAGTCATGCAAGTGGTG	GAAGAAACTGTCCCTGGGCTCG	344
BVDV6	CTTCAGATATAAAAGATGGCTG	GCTGCCACGGTCTCATTTCTTAC	736
BVDV7	GATATCCAGGGTTGTGGCAGC	CTCCTCACTTTTAATAAACTTG	696
BVDV8	CAAGTTTATTAAAAGTGAGGAG	GTCATAGTGGTAGTCTTTTGATC	1 469
BVDV9	CGGATCAAAAGACTACCACTATG	GAAGGAAGGCTTATTTATCAC	1 049
BVDV10	CAGACAGGCAGCAGTTGACTTG	CTTCCTATTATTGAGATCCCAAC	1 572
BVDV11	GCAAGGTTAGTGGGGCAAGCAG	TATCCTTTACCCTTGGTTTCTTC	1 427
BVDV12	GTTACAGCAAGAGTAGACTAC	CTATCTCTAGTTGTTCCCATG	1 761
BVDV13	GATACTAAAAAGAATAAAGTTTC	GCTAAAGTGCTATGTGCGTTG	2 036
BVDV14	CAGAGTCAGTCTACCAATATATG	CAGTTCTGGCCATGATGTTC	1 002
BVDV15	CAACTGGCACAAGGTAACTG	TCTGTAGGCAACTTTCATC	1 351
BVDV16	GAGTCATCCAATACCCTGAAGC	TGAGGTCTTTTCCTAGTCCAAC	1 524
BVDV17	CATTACTAAGGTAATGTACAAC	GTTGATTGTAGTGTTATCTTGAG	1 423
BVDV18	CAGAGGGCCGGTCAACTATAAG	CAGTTTTCCAGGATTGAGCATC	1 586
BVDV19	CACATTAACATAATGGACAAGC	CTTTCAAATCCCCTATTCTTAAG	816
BVDV20	GAGAGGATGAAAGTTGCCTAC	GAGGTCTTTTCCTAGTCCAAC	930

引物名称 Primer name	正向引物 Forward primer sequence (5'→3')	反向引物 Reverse primer sequence (5'→3')	产物长度 Product length/bp
BVDV1	TGAATCCCCTTCAGCGAAGGC	CATGTGCCATGTACAGCAGAG	319
BVDV2	CTAAAAATCTCTGCTGTACATG	CTGGTGGCTTGTTCTTGTTGTG	759
BVDV3	GAACAAGCCACCAGAGTCACGC	GTTCTTTAAACAGCACATCAC	596
BVDV4	CGATAGTAGTGGATGGGGTCAAG	TGTTACAAGAAGTGTCATCTAC	2 184
BVDV5	CGTCGAGTCATGCAAGTGGTG	GAAGAAACTGTCCCTGGGCTCG	344
BVDV6	CTTCAGATATAAAAGATGGCTG	GCTGCCACGGTCTCATTTCTTAC	736
BVDV7	GATATCCAGGGTTGTGGCAGC	CTCCTCACTTTTAATAAACTTG	696
BVDV8	CAAGTTTATTAAAAGTGAGGAG	GTCATAGTGGTAGTCTTTTGATC	1 469
BVDV9	CGGATCAAAAGACTACCACTATG	GAAGGAAGGCTTATTTATCAC	1 049
BVDV10	CAGACAGGCAGCAGTTGACTTG	CTTCCTATTATTGAGATCCCAAC	1 572
BVDV11	GCAAGGTTAGTGGGGCAAGCAG	TATCCTTTACCCTTGGTTTCTTC	1 427
BVDV12	GTTACAGCAAGAGTAGACTAC	CTATCTCTAGTTGTTCCCATG	1 761
BVDV13	GATACTAAAAAGAATAAAGTTTC	GCTAAAGTGCTATGTGCGTTG	2 036
BVDV14	CAGAGTCAGTCTACCAATATATG	CAGTTCTGGCCATGATGTTC	1 002
BVDV15	CAACTGGCACAAGGTAACTG	TCTGTAGGCAACTTTCATC	1 351
BVDV16	GAGTCATCCAATACCCTGAAGC	TGAGGTCTTTTCCTAGTCCAAC	1 524
BVDV17	CATTACTAAGGTAATGTACAAC	GTTGATTGTAGTGTTATCTTGAG	1 423
BVDV18	CAGAGGGCCGGTCAACTATAAG	CAGTTTTCCAGGATTGAGCATC	1 586
BVDV19	CACATTAACATAATGGACAAGC	CTTTCAAATCCCCTATTCTTAAG	816
BVDV20	GAGAGGATGAAAGTTGCCTAC	GAGGTCTTTTCCTAGTCCAAC	930

长度Length/nt	21NM-44	21NX-53	21SD-16
5'UTR	283	282	153
3'UTR	208	315	217
N^pro	504	504	504
Capsid	312	312	312
E^rns	684	684	684
E1	576	576	585
E2	1 122	1 122	1 122
P7	207	207	207
NS2	1 356	1 362	1 362
NS3	2 049	2 049	2 049
NS4A	192	192	192
NS4B	1 050	1 041	1 041
NS5A	1 488	1 488	1 488
NS5B	2 160	2 160	2 160

长度Length/nt	21NM-44	21NX-53	21SD-16
5'UTR	283	282	153
3'UTR	208	315	217
N^pro	504	504	504
Capsid	312	312	312
E^rns	684	684	684
E1	576	576	585
E2	1 122	1 122	1 122
P7	207	207	207
NS2	1 356	1 362	1 362
NS3	2 049	2 049	2 049
NS4A	192	192	192
NS4B	1 050	1 041	1 041
NS5A	1 488	1 488	1 488
NS5B	2 160	2 160	2 160

Genomic features of 3 bovine viral diarrhea viruses isolated from newborn claves

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