Virulence genes and drug resistance characteristics of Escherichia coli in ready-to-eat duck products

doi:10.3969/j.issn.1004-1524.2020.10.13

›› 2020, Vol. 32 ›› Issue (10): 1841-1848.DOI: 10.3969/j.issn.1004-1524.2020.10.13

• Quality and Safety of Agriculturel Products • Previous Articles Next Articles

Virulence genes and drug resistance characteristics of Escherichia coli in ready-to-eat duck products

YANG Hua¹, MA Yan², LIU Xiuting¹, LYU Wentao¹, LU Lizhi³, XIAO Yingping^1,*

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Zhejiang Zhengming Testing Co., Ltd., Ningbo 315200, China;
3. Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2020-07-28 Online:2020-10-25 Published:2020-11-16

Abstract

Abstract: To analyze the contamination of E. coli in ready-to-eat duck products, the virulence gene status and drug resistance characteristics of E. coli, 491 samples of ready-to-eat duck products were collected from 11 provinces of Zhejiang, Shanghai, Fujian, Jiangsu, Guangdong, Beijing, Heilongjiang, Inner Mongolia, Guizhou, Hubei and Sichuan for the isolation of E. coli. The VITEK 2 COMPACT analysis system was used to identify the isolates and analyze the drug resistance profile. The virulence genes, drug-resistant genes and class I integrase genes were amplified by PCR. The results showed that a total of 109 strains of E. coli were isolated from 491 ready-to-eat duck products, and the detection rate was 22.20%. The detection rate of escV was the highest (7.34%), followed by pic(6.42%), ipaH and aggR(3.67%), stx2 (2.75%), eae, lt and stx1(1.83%). Escherichia coli showed high resistance to ampicillin of cotrimoxazole and penicillins, which were 63.30% and 61.47%, respectively. The proportion of strains with drug resistance ≥3 was 60.55%, and the highest resistance to 12 antibiotics at the same time was 2.75%. Corresponding sulfonamine-resistant genes had the highest detection rates at 100.00% and 88.07%, respectively, and the detection rates of quinolone genes qnrA and qnrS, aminoglycoside genes aadA1 and mecC were also above 35%. The detection rates of β-lactam resistant genes CTX-M, TEM, SHV, CTX-M-1, CTX-M-2, CTX-M-9 and CTX-M-25 in 24 extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli strains were 100.00%, 95.83%, 4.17%, 66.67%, 12.50%, 75.00% and 4.17%, respectively. Seven strains of E. coli among 20 integron positive Ⅰ carried genes inserted elements, among them 2 strains of E. coli type Ⅰ integron genes inserted for dfrA1-aadA1, 4 strains for dfrA12-aadA2, another strain for aadA22. It was indicated that the Escherichia coli contaminated with ready-to-eat duck products had certain virulence genes and drug resistance.

Key words: ready-to-eat duck product, Escherichia coli, drug-resistant gene, virulence gene, class Ⅰ integron

CLC Number:

TS251.5

YANG Hua, MA Yan, LIU Xiuting, LYU Wentao, LU Lizhi, XIAO Yingping. Virulence genes and drug resistance characteristics of Escherichia coli in ready-to-eat duck products[J]. , 2020, 32(10): 1841-1848.

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Virulence genes and drug resistance characteristics of Escherichia coli in ready-to-eat duck products

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