Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot

doi:10.3969/j.issn.1004-1524.2021.03.01

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 383-389.DOI: 10.3969/j.issn.1004-1524.2021.03.01

• Crop Science • Previous Articles Next Articles

Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot

YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo^*()

College of Agriculture, Guizhou University, Guiyang 550025, China

Received:2020-11-13 Online:2021-04-02 Published:2021-03-25
Contact: QIU Hongbo

Abstract

Abstract:

In this study, 23 single segment substitution lines of maize 8th chromosome were obtained by successive backcross and marker assisted selection with T32 and J51, the common inbred lines of maize in Guizhou, as donor and acceptor, respectively. Then, method of natural inoculation was used to identify the resistance to maize gray spot, and 65 pairs of SSR markers that located on the 8th chromosome with obvious polymorphic differences between two parents were used for the following detection of substitution lines. The genetic structure of donor fragments of the substitution lines was evaluated by 29 SSR markers on the 8th chromosome. The results showed that 23 chromosome single-segment substitution lines had different donor fragment sites and length of the substitution fragment was 10.40-129.70 cM, the average length was 36.99 cM, total length of the imported fragment was 850.67 cM, and coverage rate of the replacement fragments on 8th chromosomes was 80.80%. Disease-resistant result showed four kinds of phenotypes: resistant, moderately resistant, susceptible and highly susceptible. Among them, the line of H3 and H17 were identified as resistant, with the two years average disease grade of 2.85 and 2.90, respectively. Two resistant lines were screened out in this study, which could be used as follow-up research materials and lay a foundation for resistant maize breeding to gray leaf spot.

Key words: maize, chromosome 8, chromosome segment substitution line, gray spot disease, resistance identification

CLC Number:

S513

YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo. Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 383-389.

Figures/Tables 5

References 21

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染色体片段代换 CSILs	病级Rating									抗性评价 Resistance
	2018年 2018 year				2019年2019 year				平均 Average
	勐嘎Mengga		江东Jiangdong		勐嘎Mengga		江东Jiangdong
	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation
J51	0	9~9	0	9~9	0	9~9	0	9~9	9.00	HS
T32	0	1~1	0	1~1	0	1~1	0	1~1	1.00	HR
H1	1.00	5~7	1.34	5~7	0.98	5~7	0.98	5~7	5.90	MR
H2	1.00	7~9	1.33	5~9	0.92	7~9	0	9~9	8.30	HS
H3	1.08	1~5	0.80	1~3	1.50	1~3	1.40	1~7	2.85	R
H4	1.00	7~9	0.98	7~9	0.80	7~9	0.98	7~9	7.85	HS
H5	1.20	5~9	0.60	7~9	0.80	7~9	0.92	7~9	8.60	HS
H6	0.60	7~9	0.60	7~9	0.80	7~9	0.92	7~9	8.15	HS
H7	1.08	3~7	0.98	5~7	1.00	5~9	0.98	5~7	5.80	MR
H8	0.80	7~9	0	9~9	0	9~9	0.60	7~9	8.85	HS
H9	1.00	7~9	0.98	7~9	0.98	7~9	0.98	7~9	8.05	HS
H10	0.92	7~9	0	9~9	0.60	7~9	0.80	7~9	8.70	HS
H11	0.98	7~9	0.80	7~9	0.98	7~9	0.98	7~9	8.30	HS
H12	0	9~9	0.92	7~9	0.98	7~9	0.98	7~9	8.25	HS
H13	0.92	7~9	0.60	7~9	0.80	7~9	0.80	7~9	8.60	HS
H14	0.80	7~9	1.28	5~9	0.92	7~9	0.60	7~9	7.95	HS
H15	1.00	7~9	0.98	7~9	0.98	7~9	0.92	7~9	8.20	HS
H16	1.28	5~9	1.00	7~9	0.98	7~9	0.98	7~9	7.80	HS
H17	0.60	1~3	0.60	1~3	0	3~3	0	3~3	2.90	R
H18	0.60	7~9	0	7~7	1.20	5~9	0.98	5~7	6.75	S
H19	1.33	5~9	0.80	7~9	1.00	7~9	0.92	7~9	8.20	HS
H20	0.92	7~9	0.00	9~9	0.92	7~9	0.60	7~9	8.05	HS
H21	0.98	7~9	0.80	7~9	0.92	7~9	1.00	7~9	8.20	HS
H22	0.60	7~9	0.92	7~9	0.80	7~9	0.98	7~9	8.50	HS
H23	0.60	7~9	0.80	7~9	0.60	7~9	0.92	7~9	8.65	HS

染色体片段代换 CSILs	病级Rating									抗性评价 Resistance
	2018年 2018 year				2019年2019 year				平均 Average
	勐嘎Mengga		江东Jiangdong		勐嘎Mengga		江东Jiangdong
	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation	标准差s	变异范围 Range of variation
J51	0	9~9	0	9~9	0	9~9	0	9~9	9.00	HS
T32	0	1~1	0	1~1	0	1~1	0	1~1	1.00	HR
H1	1.00	5~7	1.34	5~7	0.98	5~7	0.98	5~7	5.90	MR
H2	1.00	7~9	1.33	5~9	0.92	7~9	0	9~9	8.30	HS
H3	1.08	1~5	0.80	1~3	1.50	1~3	1.40	1~7	2.85	R
H4	1.00	7~9	0.98	7~9	0.80	7~9	0.98	7~9	7.85	HS
H5	1.20	5~9	0.60	7~9	0.80	7~9	0.92	7~9	8.60	HS
H6	0.60	7~9	0.60	7~9	0.80	7~9	0.92	7~9	8.15	HS
H7	1.08	3~7	0.98	5~7	1.00	5~9	0.98	5~7	5.80	MR
H8	0.80	7~9	0	9~9	0	9~9	0.60	7~9	8.85	HS
H9	1.00	7~9	0.98	7~9	0.98	7~9	0.98	7~9	8.05	HS
H10	0.92	7~9	0	9~9	0.60	7~9	0.80	7~9	8.70	HS
H11	0.98	7~9	0.80	7~9	0.98	7~9	0.98	7~9	8.30	HS
H12	0	9~9	0.92	7~9	0.98	7~9	0.98	7~9	8.25	HS
H13	0.92	7~9	0.60	7~9	0.80	7~9	0.80	7~9	8.60	HS
H14	0.80	7~9	1.28	5~9	0.92	7~9	0.60	7~9	7.95	HS
H15	1.00	7~9	0.98	7~9	0.98	7~9	0.92	7~9	8.20	HS
H16	1.28	5~9	1.00	7~9	0.98	7~9	0.98	7~9	7.80	HS
H17	0.60	1~3	0.60	1~3	0	3~3	0	3~3	2.90	R
H18	0.60	7~9	0	7~7	1.20	5~9	0.98	5~7	6.75	S
H19	1.33	5~9	0.80	7~9	1.00	7~9	0.92	7~9	8.20	HS
H20	0.92	7~9	0.00	9~9	0.92	7~9	0.60	7~9	8.05	HS
H21	0.98	7~9	0.80	7~9	0.92	7~9	1.00	7~9	8.20	HS
H22	0.60	7~9	0.92	7~9	0.80	7~9	0.98	7~9	8.50	HS
H23	0.60	7~9	0.80	7~9	0.60	7~9	0.92	7~9	8.65	HS

株系编号 Lines number	供体片段位点 Donor fragment site	最短长度 Minimum length/cM	最长长度 Maximum length/cM	估计长度 Estimated length/cM
H1	umc2356	0	63.20	31.60
H2	umc2356-bnlg1607-umc1607-umc266d-bnlg1828	93.90	165.50	129.70
H3	umc1034-csu329	1.87	62.40	32.13
H4	umc1487	0	67.99	33.00
H5	umc2354	0	39.90	19.95
H6	umc1130-umc2367	14.40	51.60	33.00
H7	umc2218	0	64.70	32.35
H8	umc226d	0	40.30	20.15
H9	umc1414	0	80.50	40.25
H10	umc2352a-bnlg1073-umc1304	0	21.30	10.65
H11	bnlg1828-umc1807	14.80	36.81	25.81
H12	bnlg1828	0	31.01	15.51
H13	bnlg2046	0	25.90	12.95
H14	umc2354-bnlg1863-bnlg1460-umc1302	44.20	62.58	53.39
H15	phi080	0	24.30	12.15
H16	bnlg1607-umc1607	21.60	77.73	49.67
H17	umc1034-csu329-bnlg1828-umc1807-umc2354	38.68	87.60	63.14
H18	umc1384	0	56.67	28.34
H19	bnlg1828-umc1807-umc1377-bnlg1460	29.68	88.00	58.84
H20	umc1139	0	26.80	13.40
H21	umc2367-bnlg2181-umc1889-umc1316	37.20	82.80	60.00
H22	umc1807-umc2354-bnlg1863-bnlg1828-umc1807-umc1377	54.60	84.38	69.49
H23	umc1377	0	10.40	5.20

株系编号 Lines number	供体片段位点 Donor fragment site	最短长度 Minimum length/cM	最长长度 Maximum length/cM	估计长度 Estimated length/cM
H1	umc2356	0	63.20	31.60
H2	umc2356-bnlg1607-umc1607-umc266d-bnlg1828	93.90	165.50	129.70
H3	umc1034-csu329	1.87	62.40	32.13
H4	umc1487	0	67.99	33.00
H5	umc2354	0	39.90	19.95
H6	umc1130-umc2367	14.40	51.60	33.00
H7	umc2218	0	64.70	32.35
H8	umc226d	0	40.30	20.15
H9	umc1414	0	80.50	40.25
H10	umc2352a-bnlg1073-umc1304	0	21.30	10.65
H11	bnlg1828-umc1807	14.80	36.81	25.81
H12	bnlg1828	0	31.01	15.51
H13	bnlg2046	0	25.90	12.95
H14	umc2354-bnlg1863-bnlg1460-umc1302	44.20	62.58	53.39
H15	phi080	0	24.30	12.15
H16	bnlg1607-umc1607	21.60	77.73	49.67
H17	umc1034-csu329-bnlg1828-umc1807-umc2354	38.68	87.60	63.14
H18	umc1384	0	56.67	28.34
H19	bnlg1828-umc1807-umc1377-bnlg1460	29.68	88.00	58.84
H20	umc1139	0	26.80	13.40
H21	umc2367-bnlg2181-umc1889-umc1316	37.20	82.80	60.00
H22	umc1807-umc2354-bnlg1863-bnlg1828-umc1807-umc1377	54.60	84.38	69.49
H23	umc1377	0	10.40	5.20

Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot

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