Principal component analysis and cluster analysis for evaluating free amino acids of 16 pepper materials

doi:10.3969/j.issn.1004-1524.2021.04.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (4): 640-650.DOI: 10.3969/j.issn.1004-1524.2021.04.09

• Horticultural Science • Previous Articles Next Articles

Principal component analysis and cluster analysis for evaluating free amino acids of 16 pepper materials

ZHANG Ting¹(), LIU Huiqin¹, GUO Qinwei¹, LI Chaosen¹, ZHANG Xinhui¹, XIANG Xiaomin¹, ZHAO Dongfeng¹, WAN Hongjian²^,^*()

1. Quzhou Academy of Agircultural and Forestry Sciences, Quzhou 324000, China
2. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2020-07-08 Online:2021-04-25 Published:2021-04-25
Contact: WAN Hongjian

Abstract

Abstract:

In order to analyze the differences in the comprehensive quality of free amino acids in 16 different peppers, the free amino acids (FAAs) were determined by liquid chromatography-tandem mass spectrometry, and the systematic evaluation were performed by principal component analysis (PCA) and cluster analysis. The results showed that 16-17 kinds of FAAs were detected in all kinds of peppers, and the total content ranged from 10.57 to 25.68 mg·g^-1. There were significant differences in the contents of free amino acids (FAA), but no obvious difference in species in the 16 cultivars of peppers, among which the highest contents of total free amino acids, essential amino acids, limiting amino acids were observed in the cultivar D1, D4 had the highest content of taste-active amino acids. Three principal components (F₁, F₂, F₃) were extracted by PCA, which contributed cumulatively to 83.501% of the total variance. The comprehensive evaluation model was established as F=0.775F₁+0.126F₂+0.0969F₃. The comprehensive scores of D4, D1 and D2 were ranked as the top three. Cluster analysis was used to divide these daylily cultivars into 3 groups, and the results were consistent with the PCA, which reflected the distinction among different germplasms of peppers.

Key words: pepper, free amino acid, principal component analysis, cluster analysis

CLC Number:

S511

ZHANG Ting, LIU Huiqin, GUO Qinwei, LI Chaosen, ZHANG Xinhui, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Principal component analysis and cluster analysis for evaluating free amino acids of 16 pepper materials[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 640-650.

Figures/Tables 9

References 25

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材料编号 Material No.	果形 Fruit form	果色 Fruit color	来源 Origin
D1	羊角椒Horn pepper	黄白Yellow-white	地方品种Landrace
D2	羊角椒Horn pepper	黄白Yellow-white	地方品种Landrace
D3	羊角椒Horn pepper	绿色Green	地方品种Landrace
D4	羊角椒Horn pepper	深绿色Dark green	地方品种Landrace
D5	羊角椒Horn pepper	绿色Green	地方品种Landrace
D6	羊角椒Horn pepper	绿色Green	地方品种Landrace
D7	羊角椒Horn pepper	黄白Yellow-white	地方品种Landrace
L1	羊角椒Horn pepper	淡绿色Light green	高代自交系Inbred line
L2	羊角椒Horn pepper	绿色Green	高代自交系Inbred line
L3	羊角椒Horn pepper	绿色Green	高代自交系Inbred line
L4	羊角椒Horn pepper	深绿色Dark green	高代自交系Inbred line
L5	泡椒Pickled pepper	绿色Green	高代自交系Inbred line
L6	线椒Thin cayenne peper	绿色Green	高代自交系Inbred line
L7	羊角椒Horn pepper	绿色Green	高代自交系Inbred line
L8	螺丝椒Screw pepper	绿色Green	高代自交系Inbred line
L9	羊角椒Horn pepper	绿色Green	高代自交系Inbred line

材料编号 Material No.	果形 Fruit form	果色 Fruit color	来源 Origin
D1	羊角椒Horn pepper	黄白Yellow-white	地方品种Landrace
D2	羊角椒Horn pepper	黄白Yellow-white	地方品种Landrace
D3	羊角椒Horn pepper	绿色Green	地方品种Landrace
D4	羊角椒Horn pepper	深绿色Dark green	地方品种Landrace
D5	羊角椒Horn pepper	绿色Green	地方品种Landrace
D6	羊角椒Horn pepper	绿色Green	地方品种Landrace
D7	羊角椒Horn pepper	黄白Yellow-white	地方品种Landrace
L1	羊角椒Horn pepper	淡绿色Light green	高代自交系Inbred line
L2	羊角椒Horn pepper	绿色Green	高代自交系Inbred line
L3	羊角椒Horn pepper	绿色Green	高代自交系Inbred line
L4	羊角椒Horn pepper	深绿色Dark green	高代自交系Inbred line
L5	泡椒Pickled pepper	绿色Green	高代自交系Inbred line
L6	线椒Thin cayenne peper	绿色Green	高代自交系Inbred line
L7	羊角椒Horn pepper	绿色Green	高代自交系Inbred line
L8	螺丝椒Screw pepper	绿色Green	高代自交系Inbred line
L9	羊角椒Horn pepper	绿色Green	高代自交系Inbred line

编号 No.	指标 Index	天冬氨酸 Asp	苏安酸 Thr	丝氨酸 Ser	谷氨酸 Glu	甘氨酸 Gly	丙氨酸 Ala	半胱氨酸 Cys	颉氨酸 Val	甲硫氨酸 Met	异亮氨酸 Ile	亮氨酸 Leu	络氨酸 Tyr	苯丙氨酸 Phe	赖氨酸 Lys	组氨酸 His	精氨酸 Arg	脯氨酸 Pro	游离氨基酸 Total free Amino acids (TFAA)	呈味氨基酸 Taste-active amino acids (DAA)	必需氨基酸 Essential amino acids (EAA)	限制氨基酸 Limiting amino acids (LAA)
L1	含量	1.20	6.86	0.39	0.81	0.05	0.16	0.02	0.29	0.03	0.07	0.05	0.09	0.21	0.12	0.12	0.05	0.03	10.57	2.61	7.64	0.15
	Content/ (mg·kg^-1)
	百分比	11.40	64.88	3.69	7.62	0.46	1.54	0.22	2.78	0.27	0.70	0.52	0.83	2.02	1.17	1.15	0.46	0.29	—	24.71	72.33	1.44
	Proportion/%
L3	含量	1.10	7.68	0.33	0.86	0.05	0.20	0.03	0.25	0.03	0.07	0.06	0.06	0.14	0.19	0.09	0.05	0.02	11.24	2.55	8.43	0.22
	Content/ (mg·kg^-1)
	百分比	9.81	68.31	2.95	7.67	0.48	1.80	0.24	2.24	0.29	0.66	0.55	0.54	1.26	1.70	0.84	0.48	0.19	—	22.70	75.01	1.99
	Proportion/%
L8	含量	1.47	6.22	0.51	0.75	0.06	0.33	0.05	0.43	0.03	0.19	0.18	0.10	0.24	0.30	0.15	0.12	0.02	11.15	3.11	7.61	0.34
	Content/ (mg·kg^-1)
	百分比	13.19	55.78	4.54	6.70	0.52	2.92	0.43	3.89	0.31	1.70	1.65	0.85	2.19	2.71	1.31	1.11	0.19	—	27.87	68.23	3.02
	Proportion/%
D3	含量	0.81	8.81	0.33	1.14	0.03	0.14	0.03	0.24	0.02	0.07	0.06	0.06	0.13	0.20	0.18	0.05	—	12.31	2.45	9.54	0.22
	Content/ (mg·kg^-1)
	百分比	6.54	71.56	2.72	9.27	0.27	1.10	0.25	1.98	0.20	0.57	0.48	0.49	1.09	1.62	1.47	0.39	—	—	19.90	77.50	1.82
	Proportion/%
D7	含量	1.41	7.47	0.72	1.18	0.11	0.22	0.02	0.41	0.04	0.11	0.09	0.05	0.18	0.41	0.15	0.08	0.01	12.67	3.64	8.71	0.45
	Content/ (mg·kg^-1)
	百分比	11.14	58.97	5.68	9.34	0.86	1.75	0.19	3.24	0.32	0.89	0.70	0.42	1.43	3.20	1.17	0.66	—	—	28.76	68.76	3.52
	Proportion/%
D5	含量	1.48	7.52	0.85	1.36	0.08	0.35	0.03	0.33	0.02	0.09	0.05	0.05	0.16	0.16	0.10	0.07	0.01	12.70	4.12	8.32	0.18
	Content/ (mg·kg^-1)
	百分比	11.63	59.20	6.71	10.71	0.66	2.74	0.25	2.56	0.18	0.69	0.39	0.40	1.22	1.22	0.80	0.52	0.11	—	32.45	65.47	1.40
	Proportion/%
L4	含量	0.96	8.65	0.71	0.92	0.06	0.26	0.03	0.32	0.03	0.11	0.06	0.08	0.18	0.18	0.15	0.19	0.05	12.94	2.92	9.52	0.21
	Content/ (mg·kg^-1)
	百分比	7.39	66.80	5.52	7.14	0.50	2.03	0.26	2.43	0.25	0.81	0.48	0.60	1.39	1.41	1.17	1.45	0.37	—	22.57	73.58	1.66
	Proportion/%
L9	含量	1.05	9.38	0.40	0.94	0.08	0.32	0.04	0.36	0.04	0.10	0.07	0.06	0.15	0.18	0.11	0.06	0.01	13.35	2.79	10.27	0.23
	Content/ (mg·kg^-1)
编号 No.	指标 Index	天冬氨酸 Asp	苏安酸 Thr	丝氨酸 Ser	谷氨酸 Glu	甘氨酸 Gly	丙氨酸 Ala	半胱氨酸 Cys	颉氨酸 Val	甲硫氨酸 Met	异亮氨酸 Ile	亮氨酸 Leu	络氨酸 Tyr	苯丙氨酸 Phe	赖氨酸 Lys	组氨酸 His	精氨酸 Arg	脯氨酸 Pro	游离氨基酸 Total free Amino acids (TFAA)	呈味氨基酸 Taste-active amino acids (DAA)	必需氨基酸 Essential amino acids (EAA)	限制氨基酸 Limiting amino acids (LAA)
	百分比	7.90	70.30	3.00	7.03	0.64	2.36	0.29	2.67	0.30	0.74	0.49	0.45	1.09	1.39	0.84	0.42	0.10	—	20.92	76.98	1.69
	Proportion/%
L2	含量	1.22	9.66	0.59	1.19	0.10	0.29	0.03	0.30	0.03	0.07	0.05	0.08	0.18	0.15	0.13	0.05	0.01	14.14	3.39	10.45	0.19
	Content/ (mg·kg^-1)
	百分比	8.64	68.29	4.18	8.42	0.69	2.08	0.20	2.11	0.25	0.50	0.37	0.58	1.26	1.09	0.92	0.34	0.10	—	24.00	73.87	1.33
	Proportion/%
L6	含量	1.26	10.15	0.35	1.46	0.05	0.13	0.03	0.23	0.02	0.09	0.07	0.08	0.15	0.19	0.12	0.11	0.01	14.50	3.25	10.90	0.21
	Content/ (mg·kg^-1)
	百分比	8.69	70.00	2.43	10.07	0.32	0.93	0.18	1.61	0.15	0.64	0.47	0.55	1.00	1.32	0.82	0.78	0.04	—	22.43	75.20	1.48
	Proportion/%
D6	含量	2.02	9.12	0.88	1.11	0.05	0.41	0.03	0.33	0.02	0.11	0.08	0.06	0.17	0.26	0.13	0.15	0.02	14.96	4.47	10.10	0.28
	Content/ (mg·kg^-1)
	百分比	13.53	60.96	5.88	7.44	0.33	2.73	0.18	2.23	0.14	0.74	0.54	0.40	1.14	1.75	0.90	0.98	0.15	—	29.91	67.49	1.89
	Proportion/%
L5	含量	2.20	11.07	0.71	1.24	0.08	0.33	0.04	0.45	0.03	0.16	0.11	0.06	0.16	0.25	0.17	0.32	0.03	17.40	4.55	12.23	0.28
	Content/ (mg·kg^-1)
	百分比	12.62	63.60	4.10	7.11	0.44	1.89	0.25	2.59	0.17	0.93	0.66	0.33	0.89	1.41	1.00	1.86	0.15	—	26.16	70.25	1.58
	Proportion/%
L7	含量	1.42	12.89	0.73	1.45	0.13	0.59	0.05	0.41	0.03	0.11	0.10	0.07	0.24	0.29	0.15	0.15	0.05	18.87	4.32	14.08	0.32
	Content/ (mg·kg^-1)
	百分比	7.50	68.34	3.89	7.71	0.66	3.12	0.25	2.17	0.16	0.61	0.51	0.38	1.29	1.55	0.81	0.78	0.25	—	22.89	74.63	1.71
	Proportion/%
D4	含量	2.23	13.62	1.59	2.09	0.22	0.67	0.08	0.67	0.03	0.27	0.19	0.08	0.22	0.57	0.29	0.41	0.03	23.26	6.80	15.58	0.60
	Content/ (mg·kg^-1)
	百分比	9.59	58.58	6.82	8.99	0.94	2.87	0.33	2.89	0.14	1.17	0.80	0.36	0.95	2.46	1.23	1.78	0.11	—	29.22	66.98	2.60
	Proportion/%
D2	含量	1.88	17.29	0.98	1.85	0.15	0.32	0.04	0.64	0.07	0.15	0.14	0.08	0.18	0.35	0.23	0.16	0.02	24.54	5.19	18.82	0.42
	Content/ (mg·kg^-1)
	百分比	7.67	70.46	4.01	7.55	0.61	1.32	0.17	2.62	0.29	0.61	0.55	0.31	0.72	1.42	0.94	0.67	0.09	—	21.15	76.67	1.71
	Proportion/%
D1	含量	1.94	16.97	1.11	2.04	0.22	0.32	0.07	0.78	0.07	0.31	0.20	0.12	0.34	0.57	0.27	0.32	0.01	25.68	5.63	19.24	0.64
	Content/ (mg·kg^-1)
	百分比	7.55	66.09	4.34	7.95	0.87	1.23	0.26	3.04	0.26	1.22	0.79	0.48	1.32	2.22	1.06	1.26	0.04	—	21.95	74.95	2.48
	Proportion/%

编号 No.	指标 Index	天冬氨酸 Asp	苏安酸 Thr	丝氨酸 Ser	谷氨酸 Glu	甘氨酸 Gly	丙氨酸 Ala	半胱氨酸 Cys	颉氨酸 Val	甲硫氨酸 Met	异亮氨酸 Ile	亮氨酸 Leu	络氨酸 Tyr	苯丙氨酸 Phe	赖氨酸 Lys	组氨酸 His	精氨酸 Arg	脯氨酸 Pro	游离氨基酸 Total free Amino acids (TFAA)	呈味氨基酸 Taste-active amino acids (DAA)	必需氨基酸 Essential amino acids (EAA)	限制氨基酸 Limiting amino acids (LAA)
L1	含量	1.20	6.86	0.39	0.81	0.05	0.16	0.02	0.29	0.03	0.07	0.05	0.09	0.21	0.12	0.12	0.05	0.03	10.57	2.61	7.64	0.15
	Content/ (mg·kg^-1)
	百分比	11.40	64.88	3.69	7.62	0.46	1.54	0.22	2.78	0.27	0.70	0.52	0.83	2.02	1.17	1.15	0.46	0.29	—	24.71	72.33	1.44
	Proportion/%
L3	含量	1.10	7.68	0.33	0.86	0.05	0.20	0.03	0.25	0.03	0.07	0.06	0.06	0.14	0.19	0.09	0.05	0.02	11.24	2.55	8.43	0.22
	Content/ (mg·kg^-1)
	百分比	9.81	68.31	2.95	7.67	0.48	1.80	0.24	2.24	0.29	0.66	0.55	0.54	1.26	1.70	0.84	0.48	0.19	—	22.70	75.01	1.99
	Proportion/%
L8	含量	1.47	6.22	0.51	0.75	0.06	0.33	0.05	0.43	0.03	0.19	0.18	0.10	0.24	0.30	0.15	0.12	0.02	11.15	3.11	7.61	0.34
	Content/ (mg·kg^-1)
	百分比	13.19	55.78	4.54	6.70	0.52	2.92	0.43	3.89	0.31	1.70	1.65	0.85	2.19	2.71	1.31	1.11	0.19	—	27.87	68.23	3.02
	Proportion/%
D3	含量	0.81	8.81	0.33	1.14	0.03	0.14	0.03	0.24	0.02	0.07	0.06	0.06	0.13	0.20	0.18	0.05	—	12.31	2.45	9.54	0.22
	Content/ (mg·kg^-1)
	百分比	6.54	71.56	2.72	9.27	0.27	1.10	0.25	1.98	0.20	0.57	0.48	0.49	1.09	1.62	1.47	0.39	—	—	19.90	77.50	1.82
	Proportion/%
D7	含量	1.41	7.47	0.72	1.18	0.11	0.22	0.02	0.41	0.04	0.11	0.09	0.05	0.18	0.41	0.15	0.08	0.01	12.67	3.64	8.71	0.45
	Content/ (mg·kg^-1)
	百分比	11.14	58.97	5.68	9.34	0.86	1.75	0.19	3.24	0.32	0.89	0.70	0.42	1.43	3.20	1.17	0.66	—	—	28.76	68.76	3.52
	Proportion/%
D5	含量	1.48	7.52	0.85	1.36	0.08	0.35	0.03	0.33	0.02	0.09	0.05	0.05	0.16	0.16	0.10	0.07	0.01	12.70	4.12	8.32	0.18
	Content/ (mg·kg^-1)
	百分比	11.63	59.20	6.71	10.71	0.66	2.74	0.25	2.56	0.18	0.69	0.39	0.40	1.22	1.22	0.80	0.52	0.11	—	32.45	65.47	1.40
	Proportion/%
L4	含量	0.96	8.65	0.71	0.92	0.06	0.26	0.03	0.32	0.03	0.11	0.06	0.08	0.18	0.18	0.15	0.19	0.05	12.94	2.92	9.52	0.21
	Content/ (mg·kg^-1)
	百分比	7.39	66.80	5.52	7.14	0.50	2.03	0.26	2.43	0.25	0.81	0.48	0.60	1.39	1.41	1.17	1.45	0.37	—	22.57	73.58	1.66
	Proportion/%
L9	含量	1.05	9.38	0.40	0.94	0.08	0.32	0.04	0.36	0.04	0.10	0.07	0.06	0.15	0.18	0.11	0.06	0.01	13.35	2.79	10.27	0.23
	Content/ (mg·kg^-1)
编号 No.	指标 Index	天冬氨酸 Asp	苏安酸 Thr	丝氨酸 Ser	谷氨酸 Glu	甘氨酸 Gly	丙氨酸 Ala	半胱氨酸 Cys	颉氨酸 Val	甲硫氨酸 Met	异亮氨酸 Ile	亮氨酸 Leu	络氨酸 Tyr	苯丙氨酸 Phe	赖氨酸 Lys	组氨酸 His	精氨酸 Arg	脯氨酸 Pro	游离氨基酸 Total free Amino acids (TFAA)	呈味氨基酸 Taste-active amino acids (DAA)	必需氨基酸 Essential amino acids (EAA)	限制氨基酸 Limiting amino acids (LAA)
	百分比	7.90	70.30	3.00	7.03	0.64	2.36	0.29	2.67	0.30	0.74	0.49	0.45	1.09	1.39	0.84	0.42	0.10	—	20.92	76.98	1.69
	Proportion/%
L2	含量	1.22	9.66	0.59	1.19	0.10	0.29	0.03	0.30	0.03	0.07	0.05	0.08	0.18	0.15	0.13	0.05	0.01	14.14	3.39	10.45	0.19
	Content/ (mg·kg^-1)
	百分比	8.64	68.29	4.18	8.42	0.69	2.08	0.20	2.11	0.25	0.50	0.37	0.58	1.26	1.09	0.92	0.34	0.10	—	24.00	73.87	1.33
	Proportion/%
L6	含量	1.26	10.15	0.35	1.46	0.05	0.13	0.03	0.23	0.02	0.09	0.07	0.08	0.15	0.19	0.12	0.11	0.01	14.50	3.25	10.90	0.21
	Content/ (mg·kg^-1)
	百分比	8.69	70.00	2.43	10.07	0.32	0.93	0.18	1.61	0.15	0.64	0.47	0.55	1.00	1.32	0.82	0.78	0.04	—	22.43	75.20	1.48
	Proportion/%
D6	含量	2.02	9.12	0.88	1.11	0.05	0.41	0.03	0.33	0.02	0.11	0.08	0.06	0.17	0.26	0.13	0.15	0.02	14.96	4.47	10.10	0.28
	Content/ (mg·kg^-1)
	百分比	13.53	60.96	5.88	7.44	0.33	2.73	0.18	2.23	0.14	0.74	0.54	0.40	1.14	1.75	0.90	0.98	0.15	—	29.91	67.49	1.89
	Proportion/%
L5	含量	2.20	11.07	0.71	1.24	0.08	0.33	0.04	0.45	0.03	0.16	0.11	0.06	0.16	0.25	0.17	0.32	0.03	17.40	4.55	12.23	0.28
	Content/ (mg·kg^-1)
	百分比	12.62	63.60	4.10	7.11	0.44	1.89	0.25	2.59	0.17	0.93	0.66	0.33	0.89	1.41	1.00	1.86	0.15	—	26.16	70.25	1.58
	Proportion/%
L7	含量	1.42	12.89	0.73	1.45	0.13	0.59	0.05	0.41	0.03	0.11	0.10	0.07	0.24	0.29	0.15	0.15	0.05	18.87	4.32	14.08	0.32
	Content/ (mg·kg^-1)
	百分比	7.50	68.34	3.89	7.71	0.66	3.12	0.25	2.17	0.16	0.61	0.51	0.38	1.29	1.55	0.81	0.78	0.25	—	22.89	74.63	1.71
	Proportion/%
D4	含量	2.23	13.62	1.59	2.09	0.22	0.67	0.08	0.67	0.03	0.27	0.19	0.08	0.22	0.57	0.29	0.41	0.03	23.26	6.80	15.58	0.60
	Content/ (mg·kg^-1)
	百分比	9.59	58.58	6.82	8.99	0.94	2.87	0.33	2.89	0.14	1.17	0.80	0.36	0.95	2.46	1.23	1.78	0.11	—	29.22	66.98	2.60
	Proportion/%
D2	含量	1.88	17.29	0.98	1.85	0.15	0.32	0.04	0.64	0.07	0.15	0.14	0.08	0.18	0.35	0.23	0.16	0.02	24.54	5.19	18.82	0.42
	Content/ (mg·kg^-1)
	百分比	7.67	70.46	4.01	7.55	0.61	1.32	0.17	2.62	0.29	0.61	0.55	0.31	0.72	1.42	0.94	0.67	0.09	—	21.15	76.67	1.71
	Proportion/%
D1	含量	1.94	16.97	1.11	2.04	0.22	0.32	0.07	0.78	0.07	0.31	0.20	0.12	0.34	0.57	0.27	0.32	0.01	25.68	5.63	19.24	0.64
	Content/ (mg·kg^-1)
	百分比	7.55	66.09	4.34	7.95	0.87	1.23	0.26	3.04	0.26	1.22	0.79	0.48	1.32	2.22	1.06	1.26	0.04	—	21.95	74.95	2.48
	Proportion/%

材料编号 Material No.	鲜味氨基酸 Delicious amino acids	甜味氨基酸 Sweet amino acids	芳香族氨基酸 Aromatic amino acids	苦味氨基酸 Bitter amino acids
L1	2.13	7.61	0.33	0.50
L3	2.16	8.38	0.23	0.47
L8	2.52	7.28	0.39	0.97
D3	2.15	9.49	0.22	0.45
D7	3.00	8.67	0.26	0.74
D5	2.99	8.92	0.24	0.55
L4	2.06	9.89	0.29	0.70
L9	2.18	10.31	0.25	0.62
L2	2.57	10.78	0.29	0.50
L6	2.91	10.81	0.25	0.53
D6	3.40	10.61	0.26	0.69
L5	3.68	12.39	0.26	1.08
L7	3.16	14.54	0.36	0.80
D4	4.89	16.41	0.38	1.58
D2	4.08	19.00	0.29	1.17
D1	4.55	18.91	0.53	1.69

Principal component analysis and cluster analysis for evaluating free amino acids of 16 pepper materials

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氨基酸	Asp	Thr	Ser	Glu	Gly	Ala	Cys	Val	Met	Ile	Leu	Tyr	Phe	Lys	His	Arg
amino acids
Thr	0.564^*
Ser	0.779^**	0.634^**
Glu	0.629^**	0.873^**	0.791^**
Gly	0.601^**	0.795^**	0.840^**	0.859^**
Ala	0.606^**	0.43^*	0.755^**	0.502^*	0.619^*
Cys	0.592^*	0.651^**	0.745^**	0.687^**	0.826^**	0.699^**
Val	0.727^**	0.802^**	0.805^**	0.778^**	0.912^**	0.533^*	0.842^**
Met	0.291	0.722^**	0.364	0.507^*	0.658^**	0.046	0.423	0.761^**
Ile	0.704^**	0.637^**	0.748^**	0.684^**	0.820^**	0.509^*	0.912^**	0.914^**	0.542^*
Leu	0.670^**	0.576^*	0.644^**	0.579^*	0.722^**	0.488	0.859^**	0.879^**	0.560^**	0.947^**
Tyr	0.179	0.436	0.261	0.357	0.499^*	0.078	0.547^*	0.549^*	0.498^*	0.647^**	0.639^**
Phe	0.375	0.461	0.473	0.426	0.666^**	0.379	0.663^**	0.703^**	0.501^*	0.756^**	.739^**	0.834^**
Lys	0.656^**	0.647^**	0.790^**	0.752^**	0.869^**	0.531^*	0.801^**	0.887^**	0.557^**	0.897^**	0.862^**	0.464	0.665^**
His	0.613^*	0.790^**	0.780^**	0.810^**	0.829^**	0.459	0.826^**	0.885^**	0.595^*	0.850^**	0.797^**	0.526^*	0.574^*	0.860^**
Arg	0.785^**	0.634^**	0.795^**	0.679^**	0.708^**	0.598^*	0.833^**	0.772^**	0.297	0.856^**	0.750^**	0.389	0.489	0.752^**	0.811^**
Pro	0.102	0.078	0.19	-0.113	0.05	0.469	0.173	0.061	-0.079	0.027	0.042	0.088	0.223	-0.068	0.008	0.265

氨基酸 Amino acid	主成分1 Principle component 1		主成分2 Principle component 2		主成分3 Principle component 3
氨基酸 Amino acid	载荷Load	系数Coefficient	载荷Load	系数Coefficient	载荷Load	系数Coefficient
Asp	0.747	0.225	0.353	0.264	-0.189	-0.161
Thr	0.807	0.243	-0.108	-0.081	-0.244	-0.208
Ser	0.858	0.258	0.359	0.269	-0.157	-0.134
Glu	0.831	0.250	0.011	0.008	-0.399	-0.340
Gly	0.929	0.280	-0.034	-0.025	-0.110	-0.094
Ala	0.631	0.190	0.659	0.494	0.123	0.105
Cys	0.909	0.274	0.104	0.078	0.147	0.125
Val	0.969	0.292	-0.109	-0.082	-0.053	-0.045
Met	0.624	0.188	-0.546	-0.409	-0.116	-0.099
Ile	0.946	0.285	-0.088	-0.066	0.108	0.092
Leu	0.890	0.268	-0.13	-0.097	0.172	0.147
Tyr	0.588	0.177	-0.522	-0.391	0.510	0.435
Phe	0.728	0.219	-0.279	-0.209	0.523	0.446
Lys	0.915	0.275	-0.062	-0.046	-0.103	-0.088
His	0.917	0.276	-0.082	-0.061	-0.131	-0.112
Arg	0.856	0.258	0.293	0.219	0.025	0.021
Pro	0.116	0.035	0.538	0.403	0.653	0.557

编号 No.	F₁	F₂	F₃	综合值 Comprehen- sive scores	排名 Ranking
D1	7.580	-2.700	0.470	5.580	2
D2	3.260	-0.910	-1.550	2.262	3
D3	-3.000	-1.070	-1.210	-2.577	16
D4	7.000	2.170	-0.450	5.655	1
D5	-1.950	1.160	-0.990	-1.461	11
D6	-0.890	1.700	-0.350	-0.509	7
D7	-0.840	-0.600	-1.240	-0.847	8
L1	-2.750	-0.730	1.640	-2.064	14
L2	-1.760	-0.600	-0.100	-1.449	10
L3	-3.170	-0.190	0.060	-2.475	15
L4	-1.410	0.650	1.670	-0.849	9
L5	0.790	1.560	-0.490	0.761	5
L6	-2.340	-0.870	-0.780	-1.999	13
L7	1.110	1.670	1.470	1.213	4
L8	0.350	-0.850	2.240	0.381	6
L9	-1.990	-0.370	-0.390	-1.627	12

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主成分 Principal component	特征值 Characteristic value	贡献率 Contribution rate/%	累计贡献率 Accumulative contribution rate/%
1	11.04	64.92	64.92
2	1.78	10.49	75.41
3	1.38	8.09	83.50

主成分 Principal component	特征值 Characteristic value	贡献率 Contribution rate/%	累计贡献率 Accumulative contribution rate/%
1	11.04	64.92	64.92
2	1.78	10.49	75.41
3	1.38	8.09	83.50