Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail

doi:10.3969/j.issn.1004-1524.2021.08.06

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (8): 1393-1401.DOI: 10.3969/j.issn.1004-1524.2021.08.06

• Animal Science • Previous Articles Next Articles

Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail

DONG Zhihao¹(), CHEN Yu¹, HUANG Gaoxiang¹, BAI Junyan¹^,²^,^*(), LI Jingyun¹, ZHAO Shujuan¹^,², LEI Ying¹^,², WANG Xinle¹, HU Qihang¹, FAN Zhengyu¹

1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China
2. Luoyang Key Laboratory of Animal Genetics and Breeding, Luoyang 471023,China

Received:2020-08-07 Online:2021-08-25 Published:2021-08-27
Contact: BAI Junyan

Abstract

Abstract:

To analyze whether VIPR-1 gene can be used as a molecular marker or candidate gene for early growth traits of quail or not, in this study, three egg quails, namely Chinese yellow quail, Chinese white quail and Korean quail, were used as experimental materials. PCR-RFLP and sequencing were used to detect the polymorphism of exon 6-7 of VIPR-1 gene, and the correlation between the polymorphism and the early growth traits of egg quail was analyzed. The results showed that 15 SNP loci were detected in exon 6-7 of VIPR-1 gene in three egg quails, which were A94G, G97T, C201T, A204G, A233G, C260G, A299T, C354T, A355G, C378T, C407G, A425C, A543G, G585T, A586G. Three genotypes, AA (285 bp/811 bp), AG(285 bp/811 bp/1 096 bp) and GG(1 096 bp), were detected by restriction enzyme analysis of HpyCH4IV (A355G). The genotypic frequencies of AA, AG and GG were 0.227, 0.636, 0.136, 0.087, 0.609, 0.304, respectively. The frequencies of AG and GG genotypes in Korean quail were 0.235 and 0.765, respectively. At the age of 1-4 weeks, the HpyCH4IV locus of VIPR-1 gene was significantly correlated with the daily gain, body weight, tibial length, chest width, chest depth, sternal length and tibial circumference of Chinese yellow quails (P<0.05). There was a significant correlation between HpyCH4IV locus and tibial circumference of Chinese white quails (P<0.05). There was a significant correlation between HpyCH4IV locus and body weight, chest width and chest depth of Korean quail (P<0.05). At the age of 5-7 weeks, the HpyCH4IV locus of VIPR-1 gene was significantly correlated with the body weight, sternal length, tibial length, body length, chest width, chest depth, tibial circumference, daily gain and relative growth rate of Chinese yellow quails (P<0.05). There was a significant correlation between HpyCH4IV locus and daily gain and relative growth of Chinese white quails (P<0.05). There was a significant correlation between HpyCH4IV locus and body weight of Korean quail (P<0.05). In conclusion, the HpyCH4IV locus (A355G) in exon 6-7 of VIPR-1 gene has some influence on the early growth traits of quail.

Key words: egg quail, VIPR-1 gene, PCR-RFLP, early growth traits, gene polymorphism

CLC Number:

S837

DONG Zhihao, CHEN Yu, HUANG Gaoxiang, BAI Junyan, LI Jingyun, ZHAO Shujuan, LEI Ying, WANG Xinle, HU Qihang, FAN Zhengyu. Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail[J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1393-1401.

Figures/Tables 8

References 26

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品种 Species	基因型频率 Genotypic frequency			基因频率 Gene frequency		杂合度 Heterozy- gosity	有效等位基因数 Effective number of alleles	多态信息含量 Polymorphism information content	哈代温伯格平衡检验 Hardy-Weinberg equilibrium analysis
品种 Species	AA	AG	GG	A	G	杂合度 Heterozy- gosity	有效等位基因数 Effective number of alleles	多态信息含量 Polymorphism information content	哈代温伯格平衡检验 Hardy-Weinberg equilibrium analysis
中国黄羽鹌鹑	0.227	0.636	0.136	0.545	0.455	0.496	1.984	0.373	5.298(0.071)
Chinese yellow quail
中国白羽鹌鹑	0.087	0.609	0.304	0.391	0.609	0.476	1.910	0.363	5.324(0.070)
Chinese white quail
朝鲜鹌鹑	0	0.235	0.765	0.118	0.882	0.208	1.262	0.186	0.907(0.812)
Korean quail

品种 Species	基因型频率 Genotypic frequency			基因频率 Gene frequency		杂合度 Heterozy- gosity	有效等位基因数 Effective number of alleles	多态信息含量 Polymorphism information content	哈代温伯格平衡检验 Hardy-Weinberg equilibrium analysis
品种 Species	AA	AG	GG	A	G	杂合度 Heterozy- gosity	有效等位基因数 Effective number of alleles	多态信息含量 Polymorphism information content	哈代温伯格平衡检验 Hardy-Weinberg equilibrium analysis
中国黄羽鹌鹑	0.227	0.636	0.136	0.545	0.455	0.496	1.984	0.373	5.298(0.071)
Chinese yellow quail
中国白羽鹌鹑	0.087	0.609	0.304	0.391	0.609	0.476	1.910	0.363	5.324(0.070)
Chinese white quail
朝鲜鹌鹑	0	0.235	0.765	0.118	0.882	0.208	1.262	0.186	0.907(0.812)
Korean quail

品种 Species	基因型 Genotype	日增重 Daily gain/ (g·d^-1)	相对生长率 Relative growth rate/%	体重 Weight/g	胫长 Tibial length/cm	胸宽 Chest width/cm	胸深 Chest depth/cm	胸骨长 Sternal length/cm	体长 Body- length/cm	胫围 Tibial circum- ference/cm
中国黄羽鹌鹑 Chinese	AA	326.819 ±22.397 a	12.609 ±2.478 a	60.447 ±5.416 a	3.005 ±0.109 a	2.212 ±0.138 a	2.538 ±0.138 a	2.603 ±0.158 a	6.040 ±0.247 a	1.300 ±0.029 a
yellow quail	AG	275.510 ±15.992 ab	10.377 ±1.393 a	55.436 ±3.134 ab	2.928 ±0.059 ab	2.221 ±0.065 a	2.500 ±0.070 a	2.537 ±0.085 a	5.876 ±0.159 a	1.224 ±0.020 b
	GG	231.270 ±40.728 b	10.377 ±1.394 a	48.633 ±7.897 b	2.758 ±0.160 b	1.972 ±0.166 b	2.191 ±0.200 b	2.235 ±0.196 b	5.644 ±0.371 a	1.244 ±0.047 b
中国白羽鹌鹑 Chinese	AA	244.524 ±32.646 a	12.632 ±4.870 a	45.417 ±7.404 a	2.708 ±0.139 a	1.885 ±0.124 a	2.114 ±0.128 a	2.011 ±0.182 a	5.533 ±0.320 a	1.283 ±0.040 a
white quail	AG	255.340 ±15.759 a	12.582 ±1.512 a	44.812 ±3.086 a	2.766 ±0.059 a	1.881 ±0.058 a	2.195 ±0.060 a	2.191 ±0.084 a	5.724 ±0.148 a	1.198 ±0.023 b
	GG	210.476 ±19.422 a	11.228 ±2.470 a	40.971 ±3.648 a	2.657 ±0.082 a	1.827 ±0.086 a	2.151 ±0.090 a	2.063 ±0.137 a	5.652 ±0.190 a	1.157 ±0.026 b
朝鲜鹌鹑 Korean quail	AG	353.691 ±36.739 a	10.675 ±2.382 a	72.783 ±6.283 a	3.277 ±0.111 a	2.529 ±0.104 a	2.748 ±0.135 a	2.778 ±0.159 a	6.342 ±0.278 a	1.317 ±0.039 a
	GG	341.319 ±22.964 a	9.746 ±0.816 a	65.636 ±4.017 b	3.148 ±0.067 a	2.262 ±0.083 b	2.544 ±0.067 b	2.636 ±0.112 a	6.185 ±0.150 a	1.387 ±0.081 a

品种 Species	基因型 Genotype	日增重 Daily gain/ (g·d^-1)	相对生长率 Relative growth rate/%	体重 Weight/g	胫长 Tibial length/cm	胸宽 Chest width/cm	胸深 Chest depth/cm	胸骨长 Sternal length/cm	体长 Body- length/cm	胫围 Tibial circum- ference/cm
中国黄羽鹌鹑 Chinese	AA	326.819 ±22.397 a	12.609 ±2.478 a	60.447 ±5.416 a	3.005 ±0.109 a	2.212 ±0.138 a	2.538 ±0.138 a	2.603 ±0.158 a	6.040 ±0.247 a	1.300 ±0.029 a
yellow quail	AG	275.510 ±15.992 ab	10.377 ±1.393 a	55.436 ±3.134 ab	2.928 ±0.059 ab	2.221 ±0.065 a	2.500 ±0.070 a	2.537 ±0.085 a	5.876 ±0.159 a	1.224 ±0.020 b
	GG	231.270 ±40.728 b	10.377 ±1.394 a	48.633 ±7.897 b	2.758 ±0.160 b	1.972 ±0.166 b	2.191 ±0.200 b	2.235 ±0.196 b	5.644 ±0.371 a	1.244 ±0.047 b
中国白羽鹌鹑 Chinese	AA	244.524 ±32.646 a	12.632 ±4.870 a	45.417 ±7.404 a	2.708 ±0.139 a	1.885 ±0.124 a	2.114 ±0.128 a	2.011 ±0.182 a	5.533 ±0.320 a	1.283 ±0.040 a
white quail	AG	255.340 ±15.759 a	12.582 ±1.512 a	44.812 ±3.086 a	2.766 ±0.059 a	1.881 ±0.058 a	2.195 ±0.060 a	2.191 ±0.084 a	5.724 ±0.148 a	1.198 ±0.023 b
	GG	210.476 ±19.422 a	11.228 ±2.470 a	40.971 ±3.648 a	2.657 ±0.082 a	1.827 ±0.086 a	2.151 ±0.090 a	2.063 ±0.137 a	5.652 ±0.190 a	1.157 ±0.026 b
朝鲜鹌鹑 Korean quail	AG	353.691 ±36.739 a	10.675 ±2.382 a	72.783 ±6.283 a	3.277 ±0.111 a	2.529 ±0.104 a	2.748 ±0.135 a	2.778 ±0.159 a	6.342 ±0.278 a	1.317 ±0.039 a
	GG	341.319 ±22.964 a	9.746 ±0.816 a	65.636 ±4.017 b	3.148 ±0.067 a	2.262 ±0.083 b	2.544 ±0.067 b	2.636 ±0.112 a	6.185 ±0.150 a	1.387 ±0.081 a

品种 Species	基因型 Genotype	日增重 Daily gain/ (g·d^-1)	相对生长率 Relative growth rate/%	体重 Weight/g	胫长 Tibial length/cm	胸宽 Chest width/cm	胸深 Chest depth/cm	胸骨长 Sternal length/cm	体长 Body- length/cm	胫围 Tibial circum- ference/cm
中国黄羽鹌鹑 Chinese	AA	233.429 ±22.658 ab	2.407 ±0.266 ab	116.787 ±4.304 a	3.557 ±0.020 a	3.021 ±0.056 a	3.456 ±0.056 a	4.053 ±0.065 a	8.113 ±0.134 a	1.487 ±0.022 a
yellow quail	AG	175.442 ±15.250 b	2.161 ±0.217 b	99.514 ±3.046 b	3.437 ±0.029 ab	2.969 ±0.050 a	3.277 ±0.043 ab	3.796 ±0.064 b	7.783 ±0.102 ab	1.388 ±0.011 b
	GG	246.349 ±22.918 a	3.448 ±0.659 a	97.356 ±5.681 b	3.394 ±0.087 b	2.747 ±0.103 b	3.160 ±0.106 b	3.597 ±0.114 b	7.533 ±0.224 b	1.422 ±0.015 ab
中国白羽鹌鹑 Chinese	AA	157.857 ±23.453 b	2.252 ±0.358 b	86.283 ±7.724 a	3.375 ±0.092 a	2.636 ±0.124 a	3.000 ±0.127 a	3.267 ±0.247 a	7.100 ±0.369 a	1.383 ±0.040 a
white quail	AG	218.980 ±11.465 a	2.851 ±0.159 ab	94.424 ±3.635 a	3.359 ±0.023 a	2.693 ±0.049 a	3.247 ±0.061 a	3.436 ±0.064 a	7.483 ±0.102 a	1.355 ±0.024 a
	GG	213.129 ±13.816 ab	3.260 ±0.283 a	86.338 ±4.777 a	3.352 ±0.054 a	2.622 ±0.080 a	3.092 ±0.097 a	3.281 ±0.109 a	7.243 ±0.158 a	1.352 ±0.018 a
朝鲜鹌鹑 Korean quail	AG	236.190 ±46.561 a	2.193 ±0.491 a	133.875 ±5.576 a	3.848 ±0.061 a	3.249 ±0.062 a	3.798 ±0.057 a	4.233 ±0.087 a	8.783 ±0.109 a	1.492 ±0.023 a
	GG	214.908 ±18.464 a	2.137 ±0.215 a	126.803 ±2.867 b	3.769 ±0.019 a	3.172 ±0.057 a	3.617 ±0.057 a	4.177 ±0.061 a	8.615 ±0.100 a	1.492 ±0.011 a

Association analysis of VIPR-1 gene polymorphism and early growth traits in egg quail

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