Screening of salt-tolerant growth-promoting Bacillus strains and their effect on oat growth under salt stress

doi:10.3969/j.issn.1004-1524.2022.06.17

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (6): 1268-1276.DOI: 10.3969/j.issn.1004-1524.2022.06.17

• Environmental Science • Previous Articles Next Articles

Screening of salt-tolerant growth-promoting Bacillus strains and their effect on oat growth under salt stress

LI Liyan¹^,²(), TAN Haixia³^,^*(), LI Jing¹^,², WANG Lianlong³, DU Yinghui¹^,², XU Zhiwen¹

1. Leading Biological and Agricultural Limited Company, Qinhuangdao 066000, Hebei, China
2. Hebei Agricultural Biotechnology Technology Innovation Center, Qinhuangdao 066000, Hebei, China
3. Department of Ecology, Hebei University of Environmental Engineering, Qinhuangdao 066000, Hebei, China

Received:2021-07-09 Online:2022-06-25 Published:2022-06-30
Contact: TAN Haixia

Abstract

Abstract:

Abstract: In order to screen salt-tolerant growth-promoting Bacillus strains from the rhizosphere soil of coastal halophytes in Qinhuangdao City, Hebei Province, and provide bacterial resources for the development of salt-tolerant bacterial manure, high-salinity tolerant Bacillus strains were screened and separated by LB culture medium with pH of 9.0 and NaCl mass fraction of 5%, 10%, 15%, respectively. The strains with growth-promoting abilities were further screened by functional medium. Their auxin (IAA)-producing abilities were quantitatively analyzed by Salkowski colorimetric method. The pot experiment was used to study their effect on the growth of oat under salt stress, and 16S rDNA sequence analysis was used to identify the strain with good growth promoting effect. It was shown that a total of 13 Bacillus strains were isolated with high salt tolerance, among which 3 strains (YP2, YP4, SM12) could tolerant 10% (mass fraction) NaCl. All the three strains had abilities of phosphate-solubilizing, potassium-releasing, nitrogen fixation, and production of IAA. Besides, all the three strains could improve the salt resistance of oat and promote its growth under salt stress, and the strain YP2 showed the best performance. Compared with the control, the plant height, stem diameter, shoot biomass, root length, root surface area and root tips quantity were significantly (P<0.05) increased by 72.02%, 42.58%, 186.11%, 392.35%, 378.07% and 518.85%, respectively, malondialdehyde content in oat leaves was significantly (P<0.05) decreased by 43.34%, and the contents of chlorophyll and proline, and activities of peroxidase and catalase were significantly (P<0.05) increased by 312.20%, 124.10%, 274.09% and 198.60%, respectively. As revealed by 16S rDNA sequences, YP2 strain was identified as Bacillus flexus, which has great potential to be developed as a special bacterial agent.

Key words: salt tolerant growth-promoting bacteria, Bacillus, salt stress, physiological characteristics, oat

CLC Number:

S144

LI Liyan, TAN Haixia, LI Jing, WANG Lianlong, DU Yinghui, XU Zhiwen. Screening of salt-tolerant growth-promoting Bacillus strains and their effect on oat growth under salt stress[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1268-1276.

Figures/Tables 9

References 33

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盐生植物 Halophyte	pH	碱解氮 Alkaline N/ (mg·kg^-1)	有效磷 Available P/ (mg·kg^-1)	速效钾 Available K/ (mg·kg^-1)	有机质 Organic matter/ (g·kg^-1)	水溶性盐分 Water soluble salt/( g·kg^-1)
盐地碱蓬 Suaeda salsa	8.55	19	10.3	256	4.69	4.0
盐角草 Salicornia europaea	8.57	47	18.4	286	7.20	5.0
芦苇 Phragmites australis	8.69	30	15.7	197	7.04	4.1
水麦冬 Triglochin palustre	8.03	27	7.7	274	5.10	4.5

盐生植物 Halophyte	pH	碱解氮 Alkaline N/ (mg·kg^-1)	有效磷 Available P/ (mg·kg^-1)	速效钾 Available K/ (mg·kg^-1)	有机质 Organic matter/ (g·kg^-1)	水溶性盐分 Water soluble salt/( g·kg^-1)
盐地碱蓬 Suaeda salsa	8.55	19	10.3	256	4.69	4.0
盐角草 Salicornia europaea	8.57	47	18.4	286	7.20	5.0
芦苇 Phragmites australis	8.69	30	15.7	197	7.04	4.1
水麦冬 Triglochin palustre	8.03	27	7.7	274	5.10	4.5

菌株来源 Sampling site	NaCl质量分数 NaCl mass fraction/%	分离菌株数 Isolates quantity	菌株编号 Strain No.
盐地碱蓬 Suaeda salsa	5	3	YP1~YP3
	10	2	YP4~YP5
	15	1	YP6
盐角草 Salicornia europaea	5	1	YJ7
盐角草 Salicornia europaea	10	1	YJ8
芦苇 Phragmites australis	5	2	LW9~LW10
芦苇 Phragmites australis	10	1	LW11
水麦冬 Triglochin palustre	5	2	SM12~SM13

菌株来源 Sampling site	NaCl质量分数 NaCl mass fraction/%	分离菌株数 Isolates quantity	菌株编号 Strain No.
盐地碱蓬 Suaeda salsa	5	3	YP1~YP3
	10	2	YP4~YP5
	15	1	YP6
盐角草 Salicornia europaea	5	1	YJ7
盐角草 Salicornia europaea	10	1	YJ8
芦苇 Phragmites australis	5	2	LW9~LW10
芦苇 Phragmites australis	10	1	LW11
水麦冬 Triglochin palustre	5	2	SM12~SM13

菌株编号 Strain No.	IAA产生活性 IAA production activity	溶解有机磷 Organic phosphate solubilization	溶解无机磷 Inorganic phosphate solubilization	解钾 Potassium-releasing	固氮 Nitrogen fixation	IAA产生量 IAA yield/(mg·L^-1）
YP1	+	—	+	+	—	25.76
YP2	+++	++	++	++	++	62.10
YP3	+	—	—	+	+	33.27
YP4	+++	—	++	++	+	50.00
YP5	++	+	+	++	+	40.30
YP6	++	+	—	—	+	36.55
YJ7	+	—	—	—	+	28.19
YJ8	+	—	+	—	+	25.46
LW9	+	—	+	—	+	20.32
LW10	+	+	+	+	+	18.57
LW11	+	+	+	—	—	10.11
SM12	++	+	—	+	++	43.80
SM13	+	—	—	+	+	29.57

Screening of salt-tolerant growth-promoting Bacillus strains and their effect on oat growth under salt stress

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菌株编号 Strain No.	pH								NaCl质量分数NaCl mass fraction/%
菌株编号 Strain No.	3.0	4.0	5.0	6.0	7.0	8.0	9.0	10.0	0.5	1	5	10	15
YP2	—	—	++	+++	+++	+++	+++	+	+++	+++	+++	++	+
YP4	—	—	+	+++	+++	+++	++	—	+++	+++	+++	+	—
SM12	—	—	—	+++	+++	+++	++	—	+++	+++	++	+	—

处理 Treatment	株高 Plant height/cm	茎粗 Stem diameter/mm	地上部鲜重 Shoot biomass/g	总根长 Root length/cm	根系总表面积 Root surface area/cm²	根系直径 Root. diameter/mm	根尖数 Root tips quantity
CK	37.21±7.75 c	2.09±0.21 b	1.44±0.50 c	76.5±13.3 c	32.33±8.35 c	1.35±0.15 a	62.12±7.3 c
YP2	64.01±8.26 a	2.98±0.33 a	4.12±1.24 a	376.5±38.3 a	154.56±31.25 a	1.37±0.11 a	384.43±13.16 a
YP4	55.17±8.83 b	2.25±0.38 b	2.59±1.55 b	228.2±38.0 b	90.43±24.87 b	1.22±0.10 a	247.83±14.28 b
SM12	62.66±9.41 ab	2.68±0.25 a	3.44±0.85 ab	277.3±37.1 b	102.97±13.09 b	1.26±0.12 a	256.34±18.14 b

处理 Treatment	PC1得分 Score of PC1	PC2得分 Score of PC2	综合得分 Total score	排名 Rank
CK	-1.365	0.621	-0.866	4
YP2	1.018	1.010	1.016	1
YP4	0.031	-1.177	-0.156	3
SM12	0.316	-0.454	0.122	2

处理 Treatment	叶绿素 Chlorophyll/(mg·g^-1)	丙二醛Malondialdehyde/ (μmol·g^-1)	脯氨酸 Proline/(μg·g^-1)	过氧化物酶 Peroxidase/(U·g^-1·min^-1）	过氧化氢酶Catalase/(mg·g^-1·min^-1）
CK	0.82±0.03 c	15.02±0.16 a	0.83±0.04 c	163.33±8.72 c	12.13±0.21 d
YP2	3.38±0.07 a	8.51±0.44 d	1.86±0.04 a	611.00±3.21 a	36.22±0.60 a
YP4	0.91±0.12 bc	13.58±0.33 b	1.64±0.06 b	483.67±4.16 b	15.98±0.41 c
SM12	1.00±0.08 b	12.13±0.30 c	1.68±0.05 b	499.50±4.58 b	31.28±0.36 b

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