Abstract:

Plant sucrose transporter family proteins (SUC) are crucial carriers that mediate sucrose transmembrane transport. In this work, a total of 10 FtSUC genes were screened and identified based on the database of transcriptome sequencing in developing seedling of Tartary buckwheat, of which gene and encoding protein sequence were 1 436-7 109 bp and 148-605 aa, respectively. Eight FtSUCs were predicted to be located in the endoplasmic reticulum, of which sequence consistency was 34.30% including 20 conserved locis. Tartary buckwheat FtSUCs and Arabidopsis AtSUCs were classified into three types, including four FtSUCs in types Ⅰ, two FtSUCs in types Ⅱ and four FtSUCs in types Ⅲ. Tartary buckwheat FTSUCs proteins were mainly composed of α-helix, irregular coil, elongated chain and β-fold. Among them, 9 FtSUCs contain transmembrane domains. The expressing patterns of FtSUCs varied in developing stem of Tartary buckwheat seedlings. For these genes, FtPinG0002608200.01, FtPinG0001943900.01 and FtPinG0001944100.01 were highly expressed at cotyledon stage, while FtPinG0000342600.01 showed gradually increased gene expressing pattern, whereas FtPinG0001943500.01 and FtPinG0009584000.01 exhibited stable gene expressing pattern during seedling development. It was speculated that the varied gene expressing pattern of FtSUCs might perform specific functions at different developmental stages. Correlation analysis confirmed that there were significantly positive or negative correlations among several FtSUCs genes in Tartary buckwheat, suggesting that the synergistic or complementary effects might be occurred among FtSUCs genes, which together regulated the sucrose transmembrane.

Key words: Tartary buckwheat, sucrose transporter gene, bioinformatics, gene expressing pattern