Influences of cadmium stress on seedling growth of different sweet corn inbred lines and screening of associated molecular markers via simple sequence repeats

doi:10.3969/j.issn.1004-1524.2022.08.02

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (8): 1582-1590.DOI: 10.3969/j.issn.1004-1524.2022.08.02

• Crop Science • Previous Articles Next Articles

Influences of cadmium stress on seedling growth of different sweet corn inbred lines and screening of associated molecular markers via simple sequence repeats

JIANG Haoliang¹^,²(), HUANG Yun¹, LIANG Shaofang¹^,², XIE Mengchen¹^,², XU Tiancheng¹, SONG Zhiting¹^,², XIANG Wenwen¹^,², CHEN Qingchun¹^,², WAN Xiaorong¹^,², SUN Wei¹^,²^,^*()

1. College of Agriculture & Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China
2. Guangzhou Key Laboratory for Research and Development of Crop Germplasm Resources, Guangzhou 510225, China

Received:2021-09-21 Online:2022-08-25 Published:2022-08-26
Contact: SUN Wei

Abstract

Abstract:

In order to explore the responses of different sweet corn inbred lines to cadmium at seedling stage and to screen molecular markers associated with these responses, 13 sweet corn inbred lines were treated with 0.01 mol·L^-1 cadmium, comprehensive evaluation of these sweet corn inbred lines was carried out based on physiological indexes, and the associated molecular makers were identified via genomic simple sequence repeats (SSR) markers. It was shown that KY188 and M114A were sensitive materials, and T96-4, M3, T35 and T8-1 were tolerant materials by Cd tolerance analysis. Compared with the control (without Cd), the chlorophyll content of KY188 (Cd sensitive inbred line) treated with Cd significantly (P<0.05) decreased with time, and its plant height, root number, root length, fresh weight of shoot, dry weight of shoot, fresh weight of root, dry weight of root, total fresh weight of plant, total dry weight of plant were significantly (P<0.05) decreased by 18.87%, 24.32%, 21.13%, 58.86%, 2.04%, 33.77%, 26.11%, 47.11% and 14.29%, respectively. However, the tolerant inbred line T96-4 showed no significant changes in the above indexes after Cd treatment. Through correlation analysis, 9 SSR markers were screened out on the 2nd, 3rd, 4th, 5th, 6th, 8th, 9th and 10th chromosome.

Key words: cadmium stress, principal component analysis, cadmium tolerance, simple sequence repeats (SSR) molecular markers, sweet corn

CLC Number:

S332.1
S513

JIANG Haoliang, HUANG Yun, LIANG Shaofang, XIE Mengchen, XU Tiancheng, SONG Zhiting, XIANG Wenwen, CHEN Qingchun, WAN Xiaorong, SUN Wei. Influences of cadmium stress on seedling growth of different sweet corn inbred lines and screening of associated molecular markers via simple sequence repeats[J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1582-1590.

Figures/Tables 7

References 24

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性状Trait	RPH	RRL	RRN	RSFW	RRFW	RSDW	RRDW	RSFW/RRFW	RSDW/RRDW	RTDW
RRL	0.431^**
RRN	0.586^**	0.274^*
RSFW	0.845^**	0.230	0.601^**
RRFW	0.659^**	-0.009	0.503^**	0.855^**
RSDW	0.743^**	0.484^**	0.810^**	0.788^**	0.634^**
RRDW	0.087	-0.104	0.122	0.129	0.198	0.102
RSFW/RRFW	0.039	0.450^**	0.443^**	-0.071	-0.048	0.526^**	-0.027
RSDW/RRDW	-0.113	-0.125	-0.036	-0.109	-0.082	-0.103	0.954^**	-0.048
RTDW	0.371^**	-0.069	0.325^*	0.480^**	0.564^**	0.368^**	0.910^**	-0.046	0.762^**
RTFW	0.828^**	0.330^*	0.733^**	0.944^**	0.825^**	0.918^**	0.136	0.196	-0.111	0.470^**

性状Trait	RPH	RRL	RRN	RSFW	RRFW	RSDW	RRDW	RSFW/RRFW	RSDW/RRDW	RTDW
RRL	0.431^**
RRN	0.586^**	0.274^*
RSFW	0.845^**	0.230	0.601^**
RRFW	0.659^**	-0.009	0.503^**	0.855^**
RSDW	0.743^**	0.484^**	0.810^**	0.788^**	0.634^**
RRDW	0.087	-0.104	0.122	0.129	0.198	0.102
RSFW/RRFW	0.039	0.450^**	0.443^**	-0.071	-0.048	0.526^**	-0.027
RSDW/RRDW	-0.113	-0.125	-0.036	-0.109	-0.082	-0.103	0.954^**	-0.048
RTDW	0.371^**	-0.069	0.325^*	0.480^**	0.564^**	0.368^**	0.910^**	-0.046	0.762^**
RTFW	0.828^**	0.330^*	0.733^**	0.944^**	0.825^**	0.918^**	0.136	0.196	-0.111	0.470^**

自交系Inbred lines	CI₁	CI₂	CI₃	U₁	U₂	U₃	D	耐镉性Cd tolerance
KY188	-2.551	-3.822	1.704	0	0	0.975	0.099	不耐镉Not tolerant
M114A	-1.579	-1.487	-2.335	0.233	0.485	0	0.230	不耐镉Not tolerant
M103	-0.795	-0.881	-1.066	0.420	0.611	0.306	0.377	中度耐镉Medium tolerant
M119	-0.554	-0.537	-1.190	0.478	0.682	0.276	0.418	中度耐镉Medium tolerant
T52-1	-0.203	-0.418	-0.575	0.562	0.707	0.425	0.477	中度耐镉Medium tolerant
M118	-0.976	0.623	0.505	0.377	0.923	0.686	0.478	中度耐镉Medium tolerant
T96-2	-0.396	-0.148	-0.275	0.516	0.763	0.497	0.478	中度耐镉Medium tolerant
M55	-0.083	-0.205	-1.351	0.591	0.751	0.238	0.482	中度耐镉Medium tolerant
T43	-0.283	-0.205	-0.340	0.543	0.751	0.482	0.486	中度耐镉Medium tolerant
T35	0.148	0.152	0.227	0.646	0.826	0.618	0.564	高度耐镉Highly tolerant
T8-1	0.707	0.055	1.807	0.780	0.805	1.000	0.657	高度耐镉Highly tolerant
M3	1.297	0.372	-0.388	0.921	0.871	0.470	0.683	高度耐镉Highly tolerant
T96-4	1.628	0.992	-2.241	1.000	1.000	0.023	0.706	高度耐镉Highly tolerant

自交系Inbred lines	CI₁	CI₂	CI₃	U₁	U₂	U₃	D	耐镉性Cd tolerance
KY188	-2.551	-3.822	1.704	0	0	0.975	0.099	不耐镉Not tolerant
M114A	-1.579	-1.487	-2.335	0.233	0.485	0	0.230	不耐镉Not tolerant
M103	-0.795	-0.881	-1.066	0.420	0.611	0.306	0.377	中度耐镉Medium tolerant
M119	-0.554	-0.537	-1.190	0.478	0.682	0.276	0.418	中度耐镉Medium tolerant
T52-1	-0.203	-0.418	-0.575	0.562	0.707	0.425	0.477	中度耐镉Medium tolerant
M118	-0.976	0.623	0.505	0.377	0.923	0.686	0.478	中度耐镉Medium tolerant
T96-2	-0.396	-0.148	-0.275	0.516	0.763	0.497	0.478	中度耐镉Medium tolerant
M55	-0.083	-0.205	-1.351	0.591	0.751	0.238	0.482	中度耐镉Medium tolerant
T43	-0.283	-0.205	-0.340	0.543	0.751	0.482	0.486	中度耐镉Medium tolerant
T35	0.148	0.152	0.227	0.646	0.826	0.618	0.564	高度耐镉Highly tolerant
T8-1	0.707	0.055	1.807	0.780	0.805	1.000	0.657	高度耐镉Highly tolerant
M3	1.297	0.372	-0.388	0.921	0.871	0.470	0.683	高度耐镉Highly tolerant
T96-4	1.628	0.992	-2.241	1.000	1.000	0.023	0.706	高度耐镉Highly tolerant

自交系Inbred lines	处理Treatment	苗高Plant height/cm	总根数Root number	根长Root length/cm
KY188	对照Control	14.52±0.54 a	9.17±0.85 a	14.01±1.19 a
	镉胁迫Cd stress	11.78±0.61 b	6.94±0.49 b	11.05±0.64 b
T96-4	对照Control	14.87±0.61 a	7.17±0.45 a	13.45±0.89 a
	镉胁迫Cd stress	14.77±0.65 a	7.63±0.49 a	13.29±1.08 a

Influences of cadmium stress on seedling growth of different sweet corn inbred lines and screening of associated molecular markers via simple sequence repeats

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标记名称Marker name	染色体Chromosome	引物序列Primer sequences(5'→3')	P
bnlg108	2	F: GCACTCACGCGCACAGGTCA R:CGCCTGCCAAGGTACATCAC	0.001 3
bnlg1456	3	F: CTCTAGGTGGTTAAGATTAACTC R: TTCATGAGGACCGTGTTGAA	0.045 0
bnlg589	4	F: GGGTCGTTTAGGGAGGCACCTTTG R: GCGACAGACAGACAGACAAGCG	0.023 0
bnlg2305	5	F: CACCTTGAAAGCATCCTCGT R: GTATCACACCCTCTGCTGCA	0.000 2
umc1083	6	F: CTTTCCTCTCTGGAGCGTGTATTG R: ATATGTTGCAGAACCATCCAGGTC	0.005 6
umc1139	8	F: TTTGTAATATGGCGCTCGAAAACT R: GAAGACGCCTCCAAGATGGATAC	0.000 4
umc1170	9	F: TGGGTGCTAAAACGTAACAACAAA R: GAGGACGAAGCAGAAATCCTACC	0.004 2
bnlg210	10	F: GCCTCGCACCAAGACATAATA R: TGCCCCATTTGAGTAGACTTC	0.000 8
phi059	10	F: AAGCTAATTAAGGCCGGTCATCCC R: TCCGTGTACTCGGCGGACTC	0.000 2

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