Effects of feeding with maggot protein added dietaries on immune and metabolic responses in liver and serum of soft-shelled turtles Pelodiscus sinensis

doi:10.3969/j.issn.1004-1524.2022.10.11

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (10): 2172-2181.DOI: 10.3969/j.issn.1004-1524.2022.10.11

• Animal Science • Previous Articles Next Articles

Effects of feeding with maggot protein added dietaries on immune and metabolic responses in liver and serum of soft-shelled turtles Pelodiscus sinensis

LIANG Qianrong(), ZHENG Tianlun, CHEN Xiaoming, ZHU Ningyu, ZHENG Xiaoye, HE Runzhen, CAO Feifei, XUE Huili, DING Xueyan()

Zhejiang Aquatic Disease Prevention and Quarantine Center, Zhejiang Fisheries Technical Extension Center, Hangzhou 310023, China

Received:2022-02-06 Online:2022-10-25 Published:2022-10-26
Contact: DING Xueyan

Abstract

Abstract:

To assess the potential immune enhancement effects of maggot protein as fodder additive on Chinese soft-shelled turtles (Pelodiscus sinensis), in this study, two feed groups of turtles were set with maggot protein/fodder mass ratio of 5% (G2) and 10% (G3) respectively, and normal fodder feed group was taken as control (G1). Turtles were fed for three months before sample collection. Ratios of survival turtles and body weight gain as well as activities of alkline phosphatase (AKP), acid phosphatase (ACP), superoxide dismutase (SOD), glutathion peroxidase (GPX), catalase (CAT) and lysozyme (LZM) were determined, and gene expression profiles of the turtles in different feed groups were obtained and compared using transcriptome analysis. The results showed that survival ratios and average body weight gain ratios of the maggot protein added feed groups were higher than those in control group. And except for glutathione peroxidase, activities of the rest five non-specific serum immune enzymes increased in maggot protein added feed groups. Comparative analysis of transcriptome sequencing showed that there were significant differentially expressed genes between experimental groups and control group. KEGG pathway enrichment analysis showed that the differentially expressed genes were mainly enriched in two immune-related pathways: phagosome, complement and coagulation cascades. In addition, differentially expressed genes between 10% maggot protein added group and the other two groups were also enriched in several immune and metabolic regulation pathways, including cytokine-cytokine receptor interaction, alanine, aspartate and glutamate metabolism and vitamin digestion and absorption, etc. In conclusion, feeding with maggot protein added dietary could enhance the immune and metabolic activities of the soft-shelled turtles, and 10% was the optimum additive proportion in our study. Its potential mechanism might be closely related to the phagosome, complement and coagulation cascades, cytokine-cytokine receptor interaction, alanine, aspartate and glutamate metabolism and vitamin digestion and absorption pathways. Our study might provide important information for the effective evaluations of maggot protein as fodder additive of Pelodiscus sinensis.

Key words: Pelodiscus sinensis, maggot protein, immune and metabolic regulations, transcriptome sequencing

CLC Number:

S966.5

LIANG Qianrong, ZHENG Tianlun, CHEN Xiaoming, ZHU Ningyu, ZHENG Xiaoye, HE Runzhen, CAO Feifei, XUE Huili, DING Xueyan. Effects of feeding with maggot protein added dietaries on immune and metabolic responses in liver and serum of soft-shelled turtles Pelodiscus sinensis[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2172-2181.

Figures/Tables 7

References 43

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引物名称 Primer name	上游引物 Forward primer(5'→3')	下游引物 Reverse primer(5'→3')	退火温度 Annealing temperature/℃
PsMHCI	ATCCTGCGGAGCCTGGTG	TTTGTGATGGCCTGCTGATT	62
PsMHCII	AGACGACTGAGCCTACCG	AAAGAGGGTGGTAAGTGTTG	53
PsTLR2	ATCCCTCCGCATCCTCA	GACTGAGGGCTTCTACGG	58
PsATP6AP1	GTGGACCTGACGCCCTTGA	TGAACATGGTGGACTGGAC	60
PsRab5	TCAGCGGCAAGCAAGTCC	GCATTCTGTGGTTCACTCTTTGG	62
PsC3	ATGTACCTGCCCATCACGC	GCGTTCCCATGCAAGTGTC	62
PsC7	TCAGATTCGTAGAAGAACAATAGCA	TCCACCAAAACTTGCAGTATGTATA	60
PsF9	ATTTGGAGAATAGTGACGGAGTAGG	GGCTGGCGTTATAGGTGGG	61
PsMASP2	CCACTCCATTGACAACACCCTC	GCGGTTCGCCGTCAATCA	63
Psfibrinogen α	TTCAAAACTCTGCCTCAAACTCA	TCTTCCCTCCCTGAACCTTG	60
PsIL1R1	ATTACTGGACAACCACCGATTT	CTTGTTCCAATTATGAAACCACTT	59
PsTNFRSF6B	AACAGCACCCAGGAGCCA	GTTCAGCAGCACTTTGACCAG	60
PsTNFRSF14	TCTCCACTGTCTGCCTCCCT	GCTTCTGTCATCCTTTGCTCTT	60
PsASNS	ACAACAGAGGAGGAATAGAGCAAA	TCAGAGCAGACACCCAGGAAC	60
PsTCN1	GCAAATAACTCCACCATCAACC	CGCTGAGGTAATACTTCTTTTGG	59
Psβ-actin	GAGACCTGACAGACTACCT	AGGATGATGAAGCAGCAGT	58

引物名称 Primer name	上游引物 Forward primer(5'→3')	下游引物 Reverse primer(5'→3')	退火温度 Annealing temperature/℃
PsMHCI	ATCCTGCGGAGCCTGGTG	TTTGTGATGGCCTGCTGATT	62
PsMHCII	AGACGACTGAGCCTACCG	AAAGAGGGTGGTAAGTGTTG	53
PsTLR2	ATCCCTCCGCATCCTCA	GACTGAGGGCTTCTACGG	58
PsATP6AP1	GTGGACCTGACGCCCTTGA	TGAACATGGTGGACTGGAC	60
PsRab5	TCAGCGGCAAGCAAGTCC	GCATTCTGTGGTTCACTCTTTGG	62
PsC3	ATGTACCTGCCCATCACGC	GCGTTCCCATGCAAGTGTC	62
PsC7	TCAGATTCGTAGAAGAACAATAGCA	TCCACCAAAACTTGCAGTATGTATA	60
PsF9	ATTTGGAGAATAGTGACGGAGTAGG	GGCTGGCGTTATAGGTGGG	61
PsMASP2	CCACTCCATTGACAACACCCTC	GCGGTTCGCCGTCAATCA	63
Psfibrinogen α	TTCAAAACTCTGCCTCAAACTCA	TCTTCCCTCCCTGAACCTTG	60
PsIL1R1	ATTACTGGACAACCACCGATTT	CTTGTTCCAATTATGAAACCACTT	59
PsTNFRSF6B	AACAGCACCCAGGAGCCA	GTTCAGCAGCACTTTGACCAG	60
PsTNFRSF14	TCTCCACTGTCTGCCTCCCT	GCTTCTGTCATCCTTTGCTCTT	60
PsASNS	ACAACAGAGGAGGAATAGAGCAAA	TCAGAGCAGACACCCAGGAAC	60
PsTCN1	GCAAATAACTCCACCATCAACC	CGCTGAGGTAATACTTCTTTTGG	59
Psβ-actin	GAGACCTGACAGACTACCT	AGGATGATGAAGCAGCAGT	58

组别 Groups	碱性磷酸酶 Alkline phosphatase/ (U·L^-1)	酸性磷酸酶 Acid phosphatase/ (U·L^-1)	超氧化物歧化酶 Superoxide dismutase/ (U·mL^-1)	谷胱甘肽过氧化物酶 Glutathion peroxidase/ (U·mL^-1)	过氧化氢酶 Catalase/ (U·mL^-1)	溶菌酶 Lysozyme/ (U·mL^-1)
G1	45.91±1.36 c	8.21±0.78 c	227.8±3.97 b	128.39±1.28 a	1.56±0.12 c	263.84±25.33 b
G2	58.83±2.28 b	32.91±1.00 b	233.4±3.53 b	117.81±1.04 b	2.82±0.09 b	276.41±12.57 b
G3	72.61±1.21 a	36.98±1.57 a	246.6±2.45 a	106.11±1.11 c	3.91±0.07 a	361.03±29.53 a

组别 Groups	碱性磷酸酶 Alkline phosphatase/ (U·L^-1)	酸性磷酸酶 Acid phosphatase/ (U·L^-1)	超氧化物歧化酶 Superoxide dismutase/ (U·mL^-1)	谷胱甘肽过氧化物酶 Glutathion peroxidase/ (U·mL^-1)	过氧化氢酶 Catalase/ (U·mL^-1)	溶菌酶 Lysozyme/ (U·mL^-1)
G1	45.91±1.36 c	8.21±0.78 c	227.8±3.97 b	128.39±1.28 a	1.56±0.12 c	263.84±25.33 b
G2	58.83±2.28 b	32.91±1.00 b	233.4±3.53 b	117.81±1.04 b	2.82±0.09 b	276.41±12.57 b
G3	72.61±1.21 a	36.98±1.57 a	246.6±2.45 a	106.11±1.11 c	3.91±0.07 a	361.03±29.53 a

比较组 Compared groups	生物学过程 Biological Process	细胞组分 Cellular Component	分子功能 Molecular Function
G2 vs G1	氧化还原过程,RNA聚合酶Ⅱ启动子的转录正调控,生物学过程 Oxidation-reduction process, positive regulation of transcription from RNA polymerase Ⅱ promoter, biological_process	细胞质,膜整合构件,细胞核 Cytoplasm, integral component of membrane, nucleus	poly(A)结合,ATP结合, 蛋白质结合 Poly (A) binding, ATP binding, protein binding
G3 vs G1	氧化还原过程,RNA聚合酶Ⅱ启动子的转录正调控,生物学过程 Oxidation-reduction process, positive regulation of transcription from RNA polymerase Ⅱ promoter, biological_process	细胞质,细胞外泌体,膜整合构件 Cytoplasm, extracellular exosome, integral component of membrane	ATP结合,poly(A)结合,锌离子结合 ATP binding, poly (A)binding, zinc ion binding
G3 vs G2	氧化还原过程, 免疫应答, 蛋白水解 Oxidation-reduction process, immune response, proteolysis	膜整合构件,细胞质,细胞外泌体 Integral component of membrane, cytoplasm, extracellular exosome	ATP结合,锌离子结合,金属离子结合 ATP binding, zinc ion binding, metal ion binding

Effects of feeding with maggot protein added dietaries on immune and metabolic responses in liver and serum of soft-shelled turtles Pelodiscus sinensis

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