Residue and dissipation dynamics of two formulations of pyraclostrobin in strawberry

doi:10.3969/j.issn.1004-1524.20220807

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (7): 1720-1728.DOI: 10.3969/j.issn.1004-1524.20220807

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Residue and dissipation dynamics of two formulations of pyraclostrobin in strawberry

YE Hui¹^,²(), CHEN Yuting¹, LUO Yuqin², FAN Xuyan², LEI Yuan², LU Lanfei², HAO Peipei³, CHENG Youpu¹^,^*(), ZHANG Changpeng²^,^*()

1. College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin 300392, China
2. Key Laboratory for Pesticide Residue Detection, Ministry of Agriculture and Rural Affairs, Institute of Quality, Safety and Nutrition for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. Tianjin Luheng Chemical Co., Ltd., Tianjin 300450, China

Received:2022-05-31 Online:2023-07-25 Published:2023-08-17
Contact: CHENG Youpu,ZHANG Changpeng

Abstract

Abstract:

To clarify the residue patterns of pyraclostrobin on strawberry in the form of emulsifiable concentrate (EC) and microencapsulated suspension (CS), dynamic trials of pyraclostrobin residues in strawberry and soil were conducted at two doses (90 and 150 g·hm^-2) during ripening, respectively. The results showed that the minimal concentration of detection of pyraclostrobin based on QuEChERS-UPLC-MS/MS were 0.002 (strawberry and soil) and 0.005 mg·kg^-1 (leaf, stem and root), respectively. The average recoveries of pyraclostrobin (strawberry and soil) were 88%-110% with the relative standard deviations (RSDs) of 1%-9% at the spiked levels of 0.002-2 mg·kg^-1. The average recoveries (root, stem and leaf) in the range of 0.005-2 mg·kg^-1 were 74%-108% with RSDs of 2%-9%. The results of residue trials showed that the residues of pyraclostrobin in EC and CS in strawberry after 1 d of application were 0.20-0.28 mg·kg^-1 (90-150 g·hm^-2) and 0.14-0.20 mg·kg^-1 (90-150 g·hm^-2), respectively. The residues were lower than 0.5 mg·kg^-1, which were in accordance with the limit values of China and EU in strawberry. The degradation dynamics of pyraclostrobin only in strawberry, stem and leaf were in accordance with the first order kinetic model, and the half-lives in strawberry were 6.6-6.8 d (EC) and 7.7-9.8 d (CS), which were easily degradable pesticides. The trial results provided data to support the safe consumption of microencapsulated suspension dosage form of pyraclostrobin on strawberry.

Key words: pyraclostrobin, microcapsule suspension, emulsifiable concentrate, pesticide residue

CLC Number:

S481.8

YE Hui, CHEN Yuting, LUO Yuqin, FAN Xuyan, LEI Yuan, LU Lanfei, HAO Peipei, CHENG Youpu, ZHANG Changpeng. Residue and dissipation dynamics of two formulations of pyraclostrobin in strawberry[J]. Acta Agriculturae Zhejiangensis, 2023, 35(7): 1720-1728.

Figures/Tables 7

References 26

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保留时间 Retention time/min	母离子 Precursor ion(m/z)	子离子 Product ion(m/z)	滞留时间 Dwell time/s	锥孔电压 Cone voltage/V	碰撞能 Collision energy/eV
1.51	388.01	197.03^*	0.061	18	28
	388.01	163.05	0.061	18	12

保留时间 Retention time/min	母离子 Precursor ion(m/z)	子离子 Product ion(m/z)	滞留时间 Dwell time/s	锥孔电压 Cone voltage/V	碰撞能 Collision energy/eV
1.51	388.01	197.03^*	0.061	18	28
	388.01	163.05	0.061	18	12

线性范围 Linear range/ (mg·L^-1)	基质 Matrix	回归方程 Regression equation	决定系数 Coefficient of determination	基质效应 ME	检出限 LOD/ (mg·L^-1)	定量限 LOQ/ (mg·kg^-1)
0.0005~0.1	乙腈Acetonitrile	y=1 938 010.57x+4 352.84	0.999 7	—	—	—
	叶Leaf	y=2 804 465.99x+1 0761.20	0.998 9	0.87	0.000 5	0.005 0
	茎Stem	y=2 576 648.62x+3 515.89	1.000 0	0.85	0.000 5	0.005 0
	草莓Strawberry	y=1 992 976.51x+6 239.22	0.999 2	1.03	0.000 5	0.002 0
	根Root	y=2 081 043.87x+6 103.10	0.999 8	1.07	0.000 5	0.005 0
	土壤Soil	y=2 292 365.17x+7 089.11	0.999 8	1.21	0.000 5	0.002 0

线性范围 Linear range/ (mg·L^-1)	基质 Matrix	回归方程 Regression equation	决定系数 Coefficient of determination	基质效应 ME	检出限 LOD/ (mg·L^-1)	定量限 LOQ/ (mg·kg^-1)
0.0005~0.1	乙腈Acetonitrile	y=1 938 010.57x+4 352.84	0.999 7	—	—	—
	叶Leaf	y=2 804 465.99x+1 0761.20	0.998 9	0.87	0.000 5	0.005 0
	茎Stem	y=2 576 648.62x+3 515.89	1.000 0	0.85	0.000 5	0.005 0
	草莓Strawberry	y=1 992 976.51x+6 239.22	0.999 2	1.03	0.000 5	0.002 0
	根Root	y=2 081 043.87x+6 103.10	0.999 8	1.07	0.000 5	0.005 0
	土壤Soil	y=2 292 365.17x+7 089.11	0.999 8	1.21	0.000 5	0.002 0

基质 Matrix	加标水平 Spike level/ (mg·kg^-1)	日内精密度Intra-day (n=5)/%						日间精密度Inter-day (n=15)/%
		1 d		2 d		3 d
		Rec	RSD	Rec	RSD	Rec	RSD	Rec		RSD
叶Leaf	0.005	89	8	89	4	87	5		89	8
	0.01	78	4	79	3	74	3		76	3
	2	83	5	80	6	79	5		81	3
茎Stem	0.005	91	9	97	8	92	9		93	9
	0.01	91	5	81	7	87	7		97	8
	2	77	4	83	5	86	5		91	9
草莓	0.002	96	3	97	1	96	4		97	4
Strawberry	0.01	94	3	96	4	88	2		107	3
	2	97	1	91	2	94	1		96	1
根Root	0.005	106	2	108	6	101	4		107	4
	0.01	96	6	94	6	101	5		105	5
	2	93	2	92	3	92	3		94	2
土壤Soil	0.002	110	9	105	6	101	6		104	7
	0.01	99	7	103	6	104	7		102	6
	2	99	4	95	3	97	4		94	4

Residue and dissipation dynamics of two formulations of pyraclostrobin in strawberry

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剂型 Formulation	剂量 Dose/ (g·hm^-2)	时间 Time	草莓 Strawberry/ (mg·kg^-1)	叶 Leaf/ (mg·kg^-1)	茎 Stem/ (mg·kg^-1)	根 Root/ (mg·kg^-1)	土壤 Soil/ (mg·kg^-1)
乳油EC	90	2 h	0.25±0.01	7.83±0.12	1.64±0.04	0.11±0.01	0.02±0
		1 d	0.20±0.01	6.52±0.05	1.33±0.04	0.17±0.01	0.01±0
		3 d	0.14±0.01	5.53±0.01	1.27±0.02	0.07±0	0.01±0
		5 d	0.11±0	5.12±0.01	0.95±0.04	0.06±0	0.01±0
		7 d	0.10±0.01	4.69±0.30	0.87±0.66	0.04±0	0.01±0
		10 d	0.08±0.01	4.33±0.08	0.72±0.08	0.05±0	0.02±0
	150	2 h	0.27±0.01	9.75±0.05	2.12±0.07	0.22±0.01	0.02±0
		1 d	0.28±0.01	8.80±0.10	2.28±0.08	0.16±0	0.01±0
		3 d	0.25±0.01	8.12±0.14	1.83±0.07	0.11±0	0.02±0
		5 d	0.19±0.01	8.03±0.16	1.56±0.09	0.10±0.01	0.02±0
		7 d	0.14±0	7.30±0.29	1.12±0.06	0.08±0	0.02±0
		10 d	0.11±0	6.42±0.19	1.07±0.09	0.05±0	0.02±0
微囊悬浮剂CS	90	2 h	0.16±0	5.94±0.10	1.09±0.03	0.07±0	0.02±0
		1 d	0.14±0.01	5.10±0.01	0.92±0.09	0.07±0	0.02±0
		3 d	0.10±0.01	4.97±0.06	0.76±0.03	0.09±0	0.01±0
		5 d	0.10±0.01	4.62±0.16	0.69±0.06	0.05±0	0.02±0
		7 d	0.09±0.01	4.56±0.01	0.65±0.05	0.07±0.01	0.01±0
		10 d	0.06±0.01	3.57±0.01	0.60±0.03	0.03±0	0.02±0
	150	2 h	0.21±0.01	8.90±0.17	1.68±0.03	0.10±0.01	0.02±0
		1 d	0.20±0.01	8.53±0.14	1.62±0.06	0.14±0	0.03±0
		3 d	0.16±0.01	7.37±0.30	1.22±0.05	0.13±0.01	0.01±0
		5 d	0.16±0.01	7.25±0.22	1.16±0.01	0.07±0.01	0.02±0
		7 d	0.12±0.01	7.16±0.35	1.17±0.01	0.05±0.01	0.03±0
		10 d	0.10±0.01	6.48±0.35	0.94±0.06	0.04±0	0.03±0

剂型 Formulation	样品 Sample	回归方程 Regression equation		决定系数 Determination coefficient(R²)		半衰期 Half-life/d
剂型 Formulation	样品 Sample	90 g·hm^-2	150 g·hm^-2	90 g·hm^-2	150 g ·hm^-2	90 g ·hm^-2	150 g ·hm^-2
乳油EC	草莓Strawberry	C_t=0.212 6e^-0.1051^t	C_t=0.301 8e^-0.1015^t	0.923 5	0.925 6	6.6	6.8
	茎Stem	C_t=1.581 2e^-0.0814^t	C_t=2.268 7e^-0.0813^t	0.965 5	0.926 4	8.5	8.6
	叶Leaf	C_t=7.05 0e^-0.0550^t	C_t=9.419 3e^-0.0380^t	0.890 3	0.951 1	12.6	18.2
微囊悬浮剂	草莓Strawberry	C_t=0.148 7e^-0.0895^t	C_t=0.208 3e^-0.0708^t	0.929 3	0.947 4	7.7	9.8
CS	茎Stem	C_t=0.978 1e^-0.0558^t	C_t=1.615 6e^-0.0540^t	0.877 5	0.895 7	12.4	12.5
	叶Leaf	C_t=5.690 2e^-0.0423^t	C_t=8.612 2e^-0.0297^t	0.894 3	0.896 2	16.5	23.1

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