Effect of application of lime with Chinese milk vetch on the cadmium uptake in rice

doi:10.3969/j.issn.1004-1524.20230477

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (3): 600-612.DOI: 10.3969/j.issn.1004-1524.20230477

• Environmental Science • Previous Articles Next Articles

Effect of application of lime with Chinese milk vetch on the cadmium uptake in rice

DONG Aiqin¹^,²(), CHEN Yuanhua¹^,², YANG Tao¹^,², XU Changxu¹^,², CHENG Liqun¹, XIE Jie¹^,²^,^*()

1. National Agricultural Experimental Station for Agricultural Environment, Yichun, National Engineering and Technology Research Center for Red Soil Improvement, Soil and Fertilizer & Resources and Environment Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
2. Jinggangshan Institute of Red Soil, Ji’an 343016, Jiangxi, China

Received:2023-04-12 Online:2024-03-25 Published:2024-04-09

Abstract

Abstract:

In the present study, a pot experiment was conducted to reveal the effects of application of Chinese milk vetch (GM), as well as the combination of Chinese milk vetch and lime (GM0HL), Chinese milk vetch and lime late application (GM5HL), Chinese milk vetch and reduced lime late application (GM5LL) on soil properties, root iron plaque and the uptake and transport of Cd in rice plants. The results showed that the soil organic matter content was significantly (P<0.05) increased by 2.1-2.7 g·kg^-1 after turning over of Chinese milk vetch than that of control (CK). Compared with the GM treatment, the soil pH was significantly increased by 0.38-0.78 pH units with application of lime, and the soil available Cd content was significantly decreased by 21.71%-41.28%. In the late stage of rapid decomposition of Chinese milk vetch, soil Eh of the treatments with lime (GM0HL, GM5HL, GM5LL) was significantly higher than that of GM. Compared with the CK treatment, the Fe content in root iron plaque of rice at the tillering stage was significantly reduced by 43.34%, 38.82% and 29.00% in GM0HL, GM5HL and GM5LL treatments, respectively, and the Cd content in root iron plaque was significantly decreased by 43.72%, 35.56% and 12.72%, respectively. The principal component analysis results showed that, soil pH and organic matter content were two key factors, which significantly affected the absorption of Cd by rice. Increasing of soil pH and organic matter content could reduce the availability of Cd in soil, increase the thickness of root iron plaque and the content of Cd in root iron plaque, thus inhibit Cd uptake by rice. In the experiment conditions, it could reduce Cd uptake and the cost in remediating Cd contaminated farmland by application of the reduced amount of lime 5 days after the overturning of Chinese milk vetch.

Key words: rice, cadmium, root iron plaque, Chinese milk vetch, lime

CLC Number:

S511.041

DONG Aiqin, CHEN Yuanhua, YANG Tao, XU Changxu, CHENG Liqun, XIE Jie. Effect of application of lime with Chinese milk vetch on the cadmium uptake in rice[J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 600-612.

Figures/Tables 9

Table 1 Soil pH and contents of organic matter and available Cd

处理 Teatment	pH	OM/(g·kg^-1)	A-Cd/(mg·kg^-1)
CK	5.63±0.08 c	21.7±1.3 b	0.256±0.03 ab
GM	5.67±0.17 c	23.9±1.3 a	0.281±0.03 a
GM0HL	6.38±0.33 a	24.4±1.0 a	0.165±0.02 c
GM5HL	6.41±0.17 a	24.2±0.9 a	0.170±0.02 c
GM5LL	6.01±0.04 b	23.8±1.2 a	0.220±0.01 b

Fig.1 Dynamics of soil redox potential potential (Eh) under treatments

Fig.2 Content of Fe and Cd in root iron plaque under treatments at different growth stages DCB-Fe, Fe content in root iron plaque; DCB-Cd, Cd content in root iron plaque. Bars marked without the same letters indicate significant difference within treatments at the same growth stage at P<0.05.

Fig.3 Cd contents in different parts of rice at tillering stage(A) and mature stage (B) under treatments Bars marked without the same letters indicate significant difference within treatments for the same part at P<0.05.

Table 2 Cd translocation factor of rice at tillering stage under treatments

处理 Treatment	TF₁	TF₂
CK	21.54±2.16 cd	0.77±0.12 b
GM	18.93±0.79 d	0.98±0.11 a
GM0HL	34.20±0.59 a	0.74±0.05 b
GM5HL	28.87±0.56 b	0.64±0.03 b
GM5LL	23.73±3.08 c	0.76±0.03 b

Table 3 Cd translocation factor of rice at mature stage under treatments

处理 Treatment	TF₁	TF₂	TF₃
CK	86.33±5.70 b	0.32±0.03 a	0.17±0.01 a
GM	89.63±13.22 b	0.22±0.03 c	0.16±0.01 a
GM0HL	116.18±5.20 a	0.26±0.03 bc	0.11±0.02 b
GM5HL	93.44±5.47 b	0.28±0.01 b	0.09±0.01 c
GM5LL	88.24±10.07 b	0.24±0.02 bc	0.10±0.01 bc

Table 4 Correlation within root iron plaque and Cd content in different parts of rice at different growth stages

生育期 Growth stage	变量 Variable	DCB-Cd	R-Cd	S-Cd	B-Cd
分蘖期Tillering stage	DCB-Fe	0.912^**	0.430	0.811^**	—
	DCB-Cd		0.515^*	0.792^**	—
	R-Cd			0.329	—
	S-Cd				—
成熟期Mature stage	DCB-Fe	0.465	0.299	0.650^**	0.711^**
	DCB-Cd		0.829^**	0.361	0.367
	R-Cd			0.097	0.210
	S-Cd				0.543^*

Table 5 Correlation within soil properties and root iron plaque and Cd content in iron plaque

变量	Eh	pH	OM	A-Cd	TDCB-Fe	MDCB-Fe	TDCB-Cd
Variable
pH	-0.524^*
OM	-0.694^**	0.350
A-Cd	0.376	-0.932^**	-0.245
TDCB-Fe	0.538^*	-0.867^**	-0.450	0.883^**
MDCB-Fe	0.457	-0.733^**	-0.517^*	0.673^**	0.799^**
TDCB-Cd	0.432	-0.859^**	-0.434	0.856^**	0.912^**	0.918^**
MDCB-Cd	-0.189	-0.498	-0.008	0.597^*	0.586^*	0.465	0.642^**

Fig.4 Principal component loading matrix PCA1, The 1st principle component; PCA2, The 2nd principle component; Eh, Soil oxidation-reduction potential; A-Cd, Soil available Cd content; OM, Soil organic matter content; TDCB-Fe, Fe content in root iron plaque at the tillering stage; MDCB-Fe, Fe content in root iron plaque at the mature stage; TDCB-Cd, Cd content in root iron plaque at the tillering stage; MDCB-Cd, Cd content in root iron plaque at the mature stage.

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Effect of application of lime with Chinese milk vetch on the cadmium uptake in rice

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[3]	PENG Jiacheng, WU Yue, XU Jiehao, XIA Meiwen, QI Tianpeng, XU Haisheng. Cloning of paxillin gene from Macrobrachium nipponense and effect of cadmium stress on its expression [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 247-253.
[4]	ZHENG Han, DING Wenjin, HE Zhaoliang, HOU Fan, DAI Binfeng, ZHONG Liequan, ZHANG Haipeng, YANG Yong. Research progress on effects of high temperature on growth and development of rice during panicle initiation stage and mitigation measures [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 470-480.
[5]	YANG Xifan, GUO Bin, QIU Gaoyang, LIU Junli, TONG Wenbin, YANG Haijun, ZHU Weidong, MAO Congyan. Inhibiting effects of immobilization agents on cadmium, lead and arsenic in rice production [J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 1-8.
[6]	LUO Yingjie, CUI Weijun, WANG Zhonghua, WU Yueyan, LIN Hongyou, ZHOU Jie, YAN Chengqi, WANG Xuming. Interaction analysis between rice ubiquitin ligase D3 and the disease resistance associated protein VOZ2 [J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 9-17.
[7]	ZHANG Siyu, LIN Chaoyang, YE Yuxuan, SHEN Zhicheng. Characterization of transgenic insect resistance and glyphosate tolerance rice expressing cry1Ab-vip3Af2 and cp4-epsps [J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1823-1833.
[8]	WANG Xintong, WAN Zuliang, YANG Zhenzhong, WANG Guojiao. Effects of rice straw returning to fields by wet harrow in autumn on leaf-soil ecological stoichiometry of rice at different growth stages [J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1243-1252.
[9]	ZHANG Chaozheng, ZHANG Xupeng, CHEN Danling. Does labor force aging and cultivated land fragmentation increase rice production cost?: based on microscopic investigation in southeast Hubei Province, China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1211-1222.
[10]	XIA Xiaodong, ZHANG Xiaobo, SHI Yongfeng, XU Rugen. Research progress in gene cloning and molecular mechanism of rice lethal mutants [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 1223-1234.
[11]	JIANG Yingying, ZHANG Hua, LEI Zhiwei, XU Heng, ZHANG Heng, ZHU Ying. OsMYC2, a key transcription factor in jasmonic acid signaling pathway, regulates the induction and differentiation of embryogenic callus in rice [J]. Acta Agriculturae Zhejiangensis, 2023, 35(5): 973-982.
[12]	MA Yihu, ZENG Xiaoyuan, HE Xianbiao, ZHOU Naidi, CHEN Jian. Response of grain yield and quality of high quality rice to climate factors at different sowing dates in southeastern Zhejiang Province, China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 736-751.
[13]	ZHANG Bin, FENG Xiaoqing, ZHENG Qian, CHEN Wen, TENG Jie. OsPUT5 silencing reduced low temperature resistance in rice [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 780-788.
[14]	LI Yanan, YE Wenxing, ZHU Xiangde, CHEN Lin, XU Xiaofeng, ZHANG Lili. LC-MS/MS-based study on effect of rice straw instead of partial corn silage on plasma metabolites of dairy cows [J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 266-274.
[15]	WU Shaofu, NI Yuanjun, ZHAN Lichuan, PENG Lu, WU Yingjie. Effects of different soil amendments on safe production and iron and zinc contents of rice in cadmium and mercury compound polluted soil [J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 417-424.