Abstract:

Bioinformatics methods were used to screen and identify the members of the GH3 gene family of Dendrobium officinale, and analyze their gene structure, protein physical and chemical properties, subcellular localization, phylogeny, protein secondary structure and transmembrane prediction, motifs, and chromosome localization. We analyzed the expression patterns of the GH3 gene family in different tissues and under low and normal phosphorus levels. Thirteen GH3 family genes were screened from the genome of D. officinale, with one gene exhibiting variable splicing and expressing 14 GH3 proteins. GH3 gene family proteins can be divided into three subfamilies, in which different genes are relatively conservative. Each member gene contains intron and exon, the phase of intron is between 0 and 2, and 13 genes are distributed on chromosome 8. The GH3 gene is expressed in all 8 tissues of D. officinale, with high expression levels in white roots and flower buds, and low expression levels in stems and lips. It is speculated that the GH3 gene may be involved in the root growth and flowering stage of D. officinale. The expression results of low and normal phosphorus levels showed that under low phosphorus levels, the expression of genes LOC110110392 and LOC110100144 in leaves was upregulated, while the expression of genes LOC110095762 and LOC110107269 in roots was upregulated. The bioinformatics of the members of the glycoside hydrolase GH3 gene family of D. officinale, the expression level in different tissues and under normal phosphorus and low phosphorus conditions were studied and analyzed, and the glycoside hydrolase genes responding to low phosphorus signals were screened, providing a research basis for the further study of glycoside hydrolase involved in the regulation mechanism of polysaccharide metabolism in D. officinale under low phosphorus conditions.

Key words: Dendrobium officinale, glycoside hydrolase GH3, bioinformatics analysis, gene expression pattern