Morphological observation and molecular identification of 11 sanghuang strains

doi:10.3969/j.issn.1004-1524.20230417

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (4): 800-810.DOI: 10.3969/j.issn.1004-1524.20230417

Morphological observation and molecular identification of 11 sanghuang strains

LU Chunxia¹^,²^,³(), LIU Kaili¹^,²^,³, LIANG Guiqiu¹^,²^,³, XIAO Xiao¹^,²^,³, WEI Wei¹^,²^,³, XU Wenwen¹^,²^,³, CHEN Jing¹^,²^,³, MO Bingqiao¹^,²^,³, LI Xiaoqun¹^,²^,³, LI Er’na⁴^,⁵^,⁶, HUANG Xuhua¹^,²^,³, LI Anhua¹^,²^,³, WEI Shini¹^,²^,³

1. Guangxi Zhuang Autonomous Region Sericultural Technology Promotion Station, Nanning 530007, China
2. Guangxi Research Academy of Sericultural Science, Nanning 530007, China
3. Guangxi Key Laboratory of Sericultural Genetic Improvement and Efficient Breeding, Nanning 530007, China
4. Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
5. Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangzhou 510610, China
6. Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China

Received:2023-03-31 Online:2024-04-25 Published:2024-04-29

Abstract

Abstract:

In order to clarify the genus of 11 similar sanghuang strains collected from different regions in China, molecular identification and morphological characterization observation were carried out. The ribosomal gene rDNA intertranscriptional spacer (ITS) technique was used to identify the genes and analyze the genetic traits of 11 sanghuang strains. Mega 7.0’s neighbor-joining (NJ) method was used to construct phylogenetic tree based on rDNA ITS sequence of 11 sanghuang strains, and the morphological observation (mycelium growth, mycelium microstructure, spore morphological characteristics, fruite body scanning structure) was analyzed and identified. The results showed that the 11 sanghuang strains were divided into two major groups, of which the strain SH-1 classified into one group, belonging to the genus Inonotus; the others strains (SH-2, SH-3, SH-4, SH-5, SH-6, SH-7, SH-8, SH-9, SH-10 and SH-11) belonged to Sanghuangporus, the strains SH-3 and SH-6 belonged to S. sanghuang, and the other 8 strains belonged to S. baumii. This method is a combination of rDNA ITS sequence analysis technology and morphological characteristics of mycelia, spore morphological characteristics and fruit-body microscopic structure characteristics of sanghuang, which can clarify the species and genetic characteristics of sanghuang, and can be used as an important theoretical basis for the artificial large-scale production of sanghuang in the future.

Key words: sanghuang, Inonotus, Sanghuangporus, Sanghuangporus sanghuang, Sanghuangporus baumii, molecular identification

CLC Number:

S567.3

LU Chunxia, LIU Kaili, LIANG Guiqiu, XIAO Xiao, WEI Wei, XU Wenwen, CHEN Jing, MO Bingqiao, LI Xiaoqun, LI Er’na, HUANG Xuhua, LI Anhua, WEI Shini. Morphological observation and molecular identification of 11 sanghuang strains[J]. Acta Agriculturae Zhejiangensis, 2024, 36(4): 800-810.

Figures/Tables 10

Table 1 Number and source of 11 sanghuang strains

菌株编号 Strain number	菌株来源 Source of strain
SH-1	湖南,桑属Hunan, Morus
SH-2	新疆,丁香属Xinjiang, Syringa
SH-3	浙江,桑属Zhejiang, Moru
SH-4	浙江,丁香属Zhejiang, Syringa
SH-5	浙江,丁香属Zhejiang, Syringa
SH-6	山东,桑属Shandong, Moru
SH-7	浙江,丁香属Zhejiang, Syringa
SH-8	吉林,丁香属Jilin, Syringa
SH-9	浙江,丁香属Zhejiang, Syringa
SH-10	湖南,丁香属Hunan, Syringa
SH-11	新疆,丁香属Xinjiang, Syringa

Fig.1 The colony morphological characteristics of sanghuang strains A, Morphology of mycelium growth for 5 d; B, Morphology of mycelium growth for 15 d.

Fig.2 Microscopic morphological characteristics of sanghuang strains mycelia

Fig.3 Fruiting body of sanghuang strains

Fig.4 Scanning electron microscope images of transverse and longitudinal sections of fruiting body of sanghuang strains

Fig.5 Micrograph of the spore of sanghuang strains

Fig.6 Agarose gel electrophoresis of genomic DNA of 11 sanghuang strains M, DNA marker.

Fig.7 Electrophoresis analysis of amplified product of sanghuang strains rDNA ITS sequences M, DNA marker.

Fig.8 Phylogenetic tree based on rDNA ITS sequences of sanghuang strains

Table 2 Genetic distance among sanghuang strains

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Morphological observation and molecular identification of 11 sanghuang strains

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