Abstract:

In order to understand the conservation effect of Gujun chicken and provide reference and help for better protection and utilization of Gujun chicken, an important local germplasm resource. Blood samples of 30 roosters and 30 hens were randomly collected from Guyuan chicken population, and the conservation of Guyuan chicken was analyzed by “Jingxin No.1” 55K chip technology. The results showed that the effective population size was 8.9, the proportion of polymorphic markers was 0.828, the expected heterozygosity was 0.360, and the observed heterozygosity was 0.359, which indicated that the genetic variation of the population was small and the uniformity was high; and the G matrix showed that there was a trend of inbreeding in the population; Among the 1 431 runs of homozygosity (ROH), the number of individuals with ROH between 50 Mb and 100 Mb was the largest, accounting for 36.67%, and the average inbreeding coefficient was 0.11. According to the results of genetic relationship analysis and cluster analysis, the 30 roosters were divided into 10 families, of which 25 hens were divided into different families, and the kinship coefficients between the other 5 hens and the tested cocks were all less than 0.1, so they were classified as “others”. To sum up, the genetic variation of the conserved Guyuan chicken population was small and the breeding degree was high, in order to reduce the inbreeding increment of the population, it was necessary to consider the genetic relationship before mating in the later conservation process.

Key words: Guyuan chicken, species conservation analysis, SNP chip, genetic relationship