Residues and dietary risk assessment of fluopyram in Dendrobium officinale

doi:10.3969/j.issn.1004-1524.20230479

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (7): 1666-1676.DOI: 10.3969/j.issn.1004-1524.20230479

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Residues and dietary risk assessment of fluopyram in Dendrobium officinale

WANG Xiaomei¹^,²(), LUO Yuqin², ZHAO Xueping², LU Lanfei¹^,², FANG Nan², WANG Xiangyun², JIANG Jinhua², HE Hongmei², ZHANG Changpeng²^,^*(), WANG Qiang¹^,²^,^*()

1. College of Life Sciences, China Jiliang University, Hangzhou 310018, China
2. Institute of Agro-Products Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-04-11 Online:2024-07-25 Published:2024-08-05

Abstract

Abstract:

A method was established for the simultaneous determination of fluopyram and its metabolite 2-(trifluoromethyl) benzamide (BZM) in both fresh and dried Dendrobium officinale. Pot experiment was conducted to investigate the residue behaviors of fluopyram and BZM in D. officinale, and the long-term dietary risk of fluopyram was evaluated. The established determination method was as follows: the samples were extracted with acetonitrile, purified with octadecyl bonded silica gel (C₁₈), primary secondary amine (PSA) and PestiCarb (PC), and detected by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The result showed that fluopyram and BZM had linearities that ranged from 0.001 to 0.1 mg·L^-1, with correlation coefficient≥ 0.998 1. The recoveries of fluopyram and BZM in D. officinale at three spiked levels (0.01, 0.1, 15 mg·kg^-1) were 85.71%-97.98%, 94.14%-112.21%, respectively, with relative standard deviations of 0.39%-5.93%, 1.22%-6.73%, respectively. When fluopyram was applied at recommended does (375.3 g·hm^-2, based on the available ingredient) or double recommended does to D. officinale, the dissipation dynamics of fluopyram fitted the first-order kinetics equation, and the half-life of fluopyram in D. officinale was 19.20-28.76 d, indicating that fluopyram fell under the category of easily degradable pesticides. The residues of fluopyram and BZM in D. officinale did not exceed 1.98, 0.09 mg·kg^-1 after 75 days of application under the experiment contions. Calculation results showed that the national estimated daily intake of fluopyram was 0.380 6 mg for the general population under the given conditions, and the corresponding dietary risk quotient was 60.41%, which indicated that applying 41.7% fluopyram suspension concentrate (SC) at the recommended dosage on D. officinale would not pose an unacceptable risk to human health.

Key words: fluopyram, Dendrobium officinale, ultra-performance liquid chromatography-tandem mass spectrometry, pesticide residue, dietary risk assessment

CLC Number:

S481.8

WANG Xiaomei, LUO Yuqin, ZHAO Xueping, LU Lanfei, FANG Nan, WANG Xiangyun, JIANG Jinhua, HE Hongmei, ZHANG Changpeng, WANG Qiang. Residues and dietary risk assessment of fluopyram in Dendrobium officinale[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1666-1676.

Figures/Tables 11

References 35

[1]	丰明凤, 高岩, 白嘉璇, 等. 铁皮石斛化学成分的分离与鉴定[J]. 沈阳药科大学学报, 2023, 40(5): 566-571.
	FENG M F, GAO Y, BAI J X, et al. Isolation and identification of the chemical constituents from Dendrobium officinale Kimura et Migo[J]. Journal of Shenyang Pharmaceutical University, 2023, 40(5): 566-571.(in Chinese with English abstract)
[2]	TANG H X, ZHAO T W, SHENG Y J, et al. Dendrobium officinale Kimura et Migo: a review on its ethnopharmacology, phytochemistry, pharmacology, and industrialization[J]. Evidence-Based Complementary and Alternative Medicine: ECAM, 2017, 2017: 7436259.
[3]	奚航献, 刘晨, 刘京晶, 等. 铁皮石斛化学成分、药理作用及其质量标志物(Q-marker)的预测分析[J]. 中草药, 2020, 51(11): 3097-3109.
	XI H X, LIU C, LIU J J, et al. Chemical components and pharmacological action for Dendrobium officinale and its prediction analysis on Q-marker[J]. Chinese Traditional and Herbal Drugs, 2020, 51(11): 3097-3109.(in Chinese with English abstract)
[4]	LIANG J, CHEN S X, HU Y D, et al. Protective roles and mechanisms of Dendrobium officinal polysaccharides on secondary liver injury in acute colitis[J]. International Journal of Biological Macromolecules, 2018, 107: 2201-2210.
[5]	WU Y Y, LIANG C Y, LIU T T, et al. Protective roles and mechanisms of polysaccharides from Dendrobium officinal on natural aging-induced premature ovarian failure[J]. Biomedicine & Pharmacotherapy, 2018, 101: 953-960.
[6]	戴德江, 沈颖, 沈瑶, 等. 浙产特色中药材病虫害化学防治的研究进展[J]. 农药学学报, 2019, 21(S1): 759-771.
	DAI D J, SHEN Y, SHEN Y, et al. Research progress on chemical control for main disease and insect pests of characteristic Chinese herbal medicines in Zhejiang Province[J]. Chinese Journal of Pesticide Science, 2019, 21(S1): 759-771.(in Chinese with English abstract)
[7]	游景茂, 郭杰, 李哲, 等. 铁皮石斛灰霉病病原分离鉴定及防治[J]. 中国中药杂志, 2019, 44(18): 3954-3959.
	YOU J M, GUO J, LI Z, et al. Isolation and identification of pathogen of Dendrobium officinale gray mold and its prevention and control[J]. China Journal of Chinese Materia Medica, 2019, 44(18): 3954-3959.(in Chinese with English abstract)
[8]	赵玲琳, 王国荣, 沈伟东, 等. 铁皮石斛炭疽病病原菌的分离鉴定及其有效杀菌剂的筛选[J]. 植物保护, 2018, 44(6): 185-190.
	ZHAO L L, WANG G R, SHEN W D, et al. Isolation and identification of the pathogen of Dendrobium officinale anthracnose and fungicide screening in laboratory[J]. Plant Protection, 2018, 44(6): 185-190.(in Chinese with English abstract)
[9]	谢显彪, 沈云峰, 杨祚斌, 等. 4种杀菌剂对紫皮石斛锈病的田间药效评价[J]. 植物保护, 2020, 46(4): 239-242.
	XIE X B, SHEN Y F, YANG Z B, et al. Field efficacy evaluation of four fungicides on dendrobium rust[J]. Plant Protection, 2020, 46(4): 239-242.(in Chinese with English abstract)
[10]	李静, 张敬泽, 吴晓鹏, 等. 铁皮石斛疫病及其病原菌[J]. 菌物学报, 2008, 27(2): 171-176.
	LI J, ZHANG J Z, WU X P, et al. The causal agent of Dendrobium candidum blight disease[J]. Mycosystema, 2008, 27(2): 171-176.(in Chinese with English abstract)
[11]	SHARMA N, MANDAL K, SHARMA S. Dissipation and risk assessment of fluopyram and trifloxystrobin on onion by GC-MS/MS[J]. Environmental Science and Pollution Research, 2022, 29(53): 80612-80623.
[12]	赵建江, 王文桥, 马志强, 等. 两种新杀菌剂对番茄灰霉病的作用方式及田间防效[J]. 中国蔬菜, 2016(5): 18-21.
	ZHAO J J, WANG W Q, MA Z Q, et al. Action mode and field control efficiency of two new fungicides against tomato gray mould[J]. China Vegetables, 2016(5): 18-21.(in Chinese with English abstract)
[13]	刘树林, 张娟, 郭飞. 50%氟吡菌酰胺悬浮剂对黄瓜灰霉病防控效果研究[J]. 现代农业科技, 2017(18): 86-87.
	LIU S L, ZHANG J, GUO F. Study on the control effect of 50% flupiromyl amide suspension concentrate on cucumber gray mold[J]. Modern Agricultural Science and Technology, 2017(18): 86-87.(in Chinese)
[14]	张晓柯, 韩絮, 马薇薇, 等. 江苏省草莓灰霉病菌对氟吡菌酰胺敏感性基线的建立及抗性风险评估[J]. 南京农业大学学报, 2015, 38(5): 810-815.
	ZHANG X K, HAN X, MA W W, et al. Baseline sensitivity of fluopyram and its resistance risk assessment against Botrytis cinerea from strawberry in Jiangsu Province[J]. Journal of Nanjing Agricultural University, 2015, 38(5): 810-815.(in Chinese with English abstract)
[15]	VARGAS-PÉREZ M, EGEA GONZÁLEZ F J, GARRIDO FRENICH A. Dissipation and residue determination of fluopyram and its metabolites in greenhouse crops[J]. Journal of the Science of Food and Agriculture, 2020, 100(13): 4826-4833.
[16]	WEI P, LIU Y N, LI W Z, et al. Metabolic and dynamic profiling for risk assessment of fluopyram, a typical phenylamide fungicide widely applied in vegetable ecosystem[J]. Scientific Reports, 2016, 6: 33898.
[17]	于福利, 付萍萍, 王素琴, 等. 番茄中氟吡菌酰胺残留量的气相色谱分析[J]. 农药, 2016, 55(4): 278-279.
	YU F L, FU P P, WANG S Q, et al. Determination of fluopyrac residues in tomato by gas chromatography[J]. Agrochemicals, 2016, 55(4): 278-279.(in Chinese with English abstract)
[18]	赵成林, 王佛娇, 程小会, 等. 41.7%氟吡菌酰胺悬浮剂的高效液相色谱分析[J]. 农药, 2020, 59(1): 27-28.
	ZHAO C L, WANG F J, CHENG X H, et al. Analysis of fluopyram 41.7% SC by HPLC[J]. Agrochemicals, 2020, 59(1): 27-28.(in Chinese with English abstract)
[19]	李文卓, 钱圆, MATSUMOTO H, 等. 气相色谱-串联质谱检测蔬菜中氟吡菌酰胺及其代谢物残留[J]. 农药学学报, 2016, 18(6): 759-764.
	LI W Z, QIAN Y, MATSUMOTO H, et al. Simultaneous determination of fluopyram and its metabolite in vegetables using gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Pesticide Science, 2016, 18(6): 759-764.(in Chinese with English abstract)
[20]	李珊, 孙志洪, 郭伟伟, 等. 气相色谱-三重四级杆串联质谱法测定土壤中氟啶虫酰胺、氟吡菌酰胺、唑虫酰胺、杀虫脲和氟虫脲[J]. 中国测试, 2022, 48(6): 64-68.
	LI S, SUN Z H, GUO W W, et al. Determination of flutamide, flupirimide, azolomide, chlorfenuron and flufenuron in soil by gas chromatography tandem mass spectrometry[J]. China Measurement & Test, 2022, 48(6): 64-68.(in Chinese with English abstract)
[21]	范金平, 张盈, 魏进, 等. QuEChERS-超高效液相色谱-串联质谱法同时测定芒果中氟吡菌酰胺、肟菌酯及其代谢物残留量[J]. 农药, 2020, 59(7): 516-522.
	FAN J P, ZHANG Y, WEI J, et al. Determination of fluopyram, trifloxystrobin and its metabolite in mango by QuEChERS-UHPLC-MS/MS[J]. Agrochemicals, 2020, 59(7): 516-522.(in Chinese with English abstract)
[22]	马琳, 陈建波, 赵莉, 等. 固相萃取-超高效液相色谱-串联质谱法同时测定果蔬中6种酰胺类农药残留量[J]. 色谱, 2015, 33(10): 1019-1025.
	MA L, CHEN J B, ZHAO L, et al. Determination of six amide pesticide residues in vegetables and fruits by solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(10): 1019-1025.(in Chinese with English abstract)
[23]	CEBALLOS-ALCANTARILLA E, AGULLÓ C, ABAD-SOMOVILLA A, et al. Highly sensitive monoclonal antibody-based immunoassays for the analysis of fluopyram in food samples[J]. Food Chemistry, 2019, 288: 117-126.
[24]	YOGENDRAIAH MATADHA N, MOHAPATRA S, SIDDAMALLAIAH L. Distribution of fluopyram and tebuconazole in pomegranate tissues and their risk assessment[J]. Food Chemistry, 2021, 358: 129909.
[25]	TRIPATHY V, SHARMA K K, MOHAPATRA S, et al. Persistence evaluation of fluopyram+tebuconazole residues on mango and pomegranate and their risk assessment[J]. Environmental Science and Pollution Research International, 2022, 29(22): 33180-33190.
[26]	吉小凤, 吕文涛, 汪建妹, 等. 超高效液相色谱-串联质谱法测定鲜鸡蛋中氟虫腈及其代谢物残留[J]. 浙江农业学报, 2020, 32(10): 1849-1854.
	JI X F, LV W T, WANG J M, et al. Determination of fipronil and its metabolites in fresh eggs by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Acta Agriculturae Zhejiangensis, 2020, 32(10): 1849-1854.(in Chinese with English abstract)
[27]	FU Y, WANG Q S, ZHANG L, et al. Dissipation, occurrence, and risk assessment of 12 pesticides in Dendrobium officinale Kimura et Migo[J]. Ecotoxicology and Environmental Safety, 2021, 222: 112487.
[28]	秦世鹏, 胡继业. 马铃薯中氟吡菌胺、氰霜唑及相关代谢物的残留量及膳食风险评估[J]. 农药, 2022, 61(7): 500-506.
	QIN S P, HU J Y. Residues and dietary risk assessment of fluopicolide, cyazofamid and related metabolites in potato[J]. Agrochemicals, 2022, 61(7): 500-506.(in Chinese with English abstract)
[29]	谭菲菲, 高政绪, 高强, 等. 氟吡菌酰胺及其代谢物在烟草种植和加工过程中的降解特征[J]. 中国烟草科学, 2021, 42(5): 69-74.
	TAN F F, GAO Z X, GAO Q, et al. Residue degradation of fluopyram and its metabolites during tobacco planting and processing[J]. Chinese Tobacco Science, 2021, 42(5): 69-74.(in Chinese with English abstract)
[30]	CHAWLA S, PATEL D J, PATEL S H, et al. Behaviour and risk assessment of fluopyram and its metabolite in cucumber (Cucumis sativus) fruit and in soil[J]. Environmental Science and Pollution Research, 2018, 25(12): 11626-11634.
[31]	林静, 张顺, 蔡挺, 等. QuEChERS-超高效液相色谱-串联质谱技术同时测定大蒜中10种农药残留[J]. 浙江农业学报, 2018, 30(1): 159-166.
	LIN J, ZHANG S, CAI T, et al. Determination of 10 pesticide residues in garlic by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry[J]. Acta Agriculturae Zhejiangensis, 2018, 30(1): 159-166.(in Chinese with English abstract)
[32]	DONG B Z, HU J Y. Dissipation and residue determination of fluopyram and tebuconazole residues in watermelon and soil by GC-MS[J]. International Journal of Environmental Analytical Chemistry, 2014, 94(5): 493-505.
[33]	MOHAPATRA S, SIDDAMALLAIAH L, BUDDIDATHI R, et al. Dissipation kinetics and risk assessment of fluopyram and tebuconazole in mango (Mangifera indica)[J]. International Journal of Environmental Analytical Chemistry, 2018, 98(3): 229-246.
[34]	PATEL B V, CHAWLA S, GOR H, et al. Residue decline and risk assessment of fluopyram+tebuconazole (400SC) in/on onion (Allium cepa)[J]. Environmental Science and Pollution Research, 2016, 23(20): 20871-20881.
[35]	FAO. Inventory of evaluations performed by the joint meeting on pesticide residue (JMPR)[DB/OL].[2023-04-11]. https://www.fao.org/pest-and-pesticide-management/guidelines-standards/faowho-joint-meeting-on-pesticide-residues-jmpr/pesticides-evaluated-by-jmpr-jmps/en/.

化合物 Compound	母离子 Precursor	子离子 Product	锥孔电压 Cone voltage/V	碰撞电压 Collision energy/V
氟吡菌酰胺Fluopyram	397.1	208.0	30	40
		173.0^*	30	30
2-(三氯甲基)苯甲酰胺	190.0	170.0^*	20	10
2-(Trifluoromethyl) benzamide		130.0	20	20

化合物 Compound	母离子 Precursor	子离子 Product	锥孔电压 Cone voltage/V	碰撞电压 Collision energy/V
氟吡菌酰胺Fluopyram	397.1	208.0	30	40
		173.0^*	30	30
2-(三氯甲基)苯甲酰胺	190.0	170.0^*	20	10
2-(Trifluoromethyl) benzamide		130.0	20	20

样品 Sample	化合物 Compound	线性范围 Linear range/ (mg·L^-1)	回归方程 Regression equation	决定系数 Determination coefficient	基质效应 Matrix effect	检出限 LOD/ (mg·L^-1)	定量限 LOQ/ (mg·kg^-1)
鲜茎	氟吡菌酰胺Fluopyram	0.001~0.1	y=4 250 459.188 1 x+3 037.381 8	0.998 1	0.93	0.001	0.001
Fresh stem	BZM	0.001~0.1	y=2 938 537.804 7 x+1 388.699 0	0.998 9	0.89	0.001	0.001
干茎	氟吡菌酰胺Fluopyram	0.001~0.1	y=1 834 865.947 1 x+394.743 1	1.000 0	0.89	0.01	0.01
Dried stem	BZM	0.001~0.1	y=1 062 565.961 4x-416.343 0	1.000 0	0.71	0.01	0.01

样品 Sample	化合物 Compound	线性范围 Linear range/ (mg·L^-1)	回归方程 Regression equation	决定系数 Determination coefficient	基质效应 Matrix effect	检出限 LOD/ (mg·L^-1)	定量限 LOQ/ (mg·kg^-1)
鲜茎	氟吡菌酰胺Fluopyram	0.001~0.1	y=4 250 459.188 1 x+3 037.381 8	0.998 1	0.93	0.001	0.001
Fresh stem	BZM	0.001~0.1	y=2 938 537.804 7 x+1 388.699 0	0.998 9	0.89	0.001	0.001
干茎	氟吡菌酰胺Fluopyram	0.001~0.1	y=1 834 865.947 1 x+394.743 1	1.000 0	0.89	0.01	0.01
Dried stem	BZM	0.001~0.1	y=1 062 565.961 4x-416.343 0	1.000 0	0.71	0.01	0.01

化合物 Compound	样品 Sample	添加水平 Spiking level/(mg·kg^-1)	回收率 Recovery/%	相对标准偏差 RSD/%
氟吡菌酰胺	鲜茎Fresh stem	0.001	85.71	5.93
Fluopyram		0.1	91.80	2.48
		15	88.10	3.62
	干茎Dried stem	0.01	93.27	0.39
		0.1	96.52	0.99
		15	97.98	2.72
BZM	鲜茎Fresh stem	0.001	94.14	6.73
		0.1	95.87	3.54
		15	107.88	2.87
	干茎Dried stem	0.01	98.88	4.31
		0.1	98.61	1.22
		15	112.21	1.93

Residues and dietary risk assessment of fluopyram in Dendrobium officinale

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样品 Sample	不同剂量下的回归方程 Regression equation under different doses		不同剂量下的半衰期 Half-life under different doses/d
样品 Sample	推荐剂量 Recommended dose	二倍推荐剂量 Double recommended does	推荐剂量 Recommended dose	二倍推荐剂量 Double recommended does
鲜茎Fresh stem	C_t=3.258 7e^{-0.036 1}^t(0.829 3)	C_t=5.126 8e^{-0.030 4}^t(0.849 4)	19.20	22.80
干茎Dried stem	C_t=7.7800e^{-0.028 2}^t(0.878 2)	C_t=9.482 2e^{-0.024 1}^t(0.908 3)	24.58	28.76

登记作物 Registration crop	食物种类 Food classification	膳食量 Dietary intake/kg	参考限量 Reference limit/(mg·kg^-1)	NEDI/mg
马铃薯Potato	薯类Tubers	0.049 5	0.03	0.001 5
辣椒Pepper	深色蔬菜Dark vegetables	0.091 5	2	0.183 0
黄瓜Cucumber	浅色蔬菜Light vegetables	0.183 7	0.5	0.091 9
葡萄Grape	水果Fruits	0.045 7	2	0.091 4
坚果Nuts	坚果Nuts	0.003 9	0.04	0.000 2
铁皮石斛Dendrobium officinale	酱油Soy sauce	0.009 0	1.41	0.012 7

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[13]	WANG Di, DI Shanshan, WANG Xinquan, ZHANG Changpeng, WANG Xiangyun, WANG Meng. Degradation of carbosulfan after its application in different growth stage of cowpea [J]. , 2020, 32(11): 2050-2058.
[14]	ZHANG Lijun, ZHANG Hu, XU Mingfei, LIN Chunmian, WU Huizhen, XU Jie, QIAN Mingrong. Dynamics of 5 fungicides residue in grape brewing fermentation process [J]. , 2019, 31(1): 149-154.
[15]	FANG Qi, ZHANG Jun, ZHOU Jinyun. Effect of processing on carbendazim residue in canned citrus [J]. , 2018, 30(9): 1599-1603.