Identification of yellow rot disease of Pleurotus pulmonarius and analysis of its pathogenicity-related transcriptome

doi:10.3969/j.issn.1004-1524.20240034

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (2): 426-437.DOI: 10.3969/j.issn.1004-1524.20240034

• Plant Protection • Previous Articles Next Articles

Identification of yellow rot disease of Pleurotus pulmonarius and analysis of its pathogenicity-related transcriptome

LU Na(), WANG Weike(), LIN Jiayao, ZHOU Zufa

Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2024-01-04 Online:2025-02-25 Published:2025-03-20
Contact: WANG Weike

Abstract

Abstract:

Yellow rot disease has a serious impact on the yield and quality of Pleurotus pulmonarius in the factory production mode. In the present study, pathogenic bacteria were isolated and purified from diseased samples. The pathogenicity of pathogenic bacteria were determinated by Koch postulates. Morphological characteristics and 16S rRNA sequences were used for pathogen identification, and their characteristic properties were analyzed by mycelium growth rate and transcriptome. It was shown that the isolated strains CAJ01-D, CAJ06-C, TXX01 and LAD07 could cause yellow rot disease, which were different species of Pseudomonas spp. The treatment using different Pseudomonas strains impacted the growth rate of P. pulmonarius mycelia, but there was no significant difference in mycelium growth vigor. There was difference in the expression of pathogenicity-related genes when P. pulmonarius was infected by Pseudomonas strains, but most of the differentially expressed genes under the infection of strains LAD07 and CAJ06-C were down-regulated. The clustering and enrichment of GO and KEGG functions showed that the cytochrome c reductase was down-regulated and mitochondrial respiration was weakened by the infection of LAD07 strain. The CAJ06-C strain caused down-regulation of glycolysis or gluconeogenesis, tyrosine metabolism and phenylalanine metabolism. It was speculated that the LAD07 and CAJ06-C strains hindered the growth and development of the fruiting body of P. pulmonarius, which was consistent with the observed severe disease symptoms, including yellowing, water stain and developmental arrest of the fruiting body under infection.

Key words: Pleurotus pulmonarius, yellow rot disease, Pseudomonas, molecular identification

CLC Number:

S432.42
S646

LU Na, WANG Weike, LIN Jiayao, ZHOU Zufa. Identification of yellow rot disease of Pleurotus pulmonarius and analysis of its pathogenicity-related transcriptome[J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 426-437.

Figures/Tables 9

Fig.1 Disease characteristics of the collected samples A, Primordia; B, Early fruiting bodies; C, Growing fruiting bodies; D, Fruiting bodies at harvest time.

Fig.2 Characteristics of disease symptoms after inoculation of strains A, Primordia; B, Early fruiting bodies; C, Growing fruiting bodies; D, Fruiting bodies at harvest time

Fig.3 Colony characteristics and cytological and ultrastructural micrographs of bacterial pathogens 1,CAJ01-D;2,CAJ06-C;3,LAD07;4,TXX01。

Fig.4 Phylogenetic tree of the isolated bacterial pathogens based on 16S rRNA

Table 1 Effect of treatments on mycelia growth of Pleurotus pulmonarius

t/d	不同病原菌处理下秀珍菇菌丝的生长速度Growth rate of mycelia under different pathogens/(cm·d^-1)
t/d	CK	CAJ01-D	CAJ06-C	LAD07	TXX01
1	0.525±0.108	0.637±0.119	0.550±0.112	0.563±0.041	0.675±0.082
2	0.485±0.043	0.375±0.043	0.475±0.147	0.450±0.050	0.450±0.050
3	0.463±0.054	0.350±0.050	0.513±0.143	0.525±0.043	0.375±0.083
4	0.528±0.079	0.350±0.031	0.520±0.097	0.510±0.038	0.453±0.084
5	0.542±0.075	0.345±0.026	0.518±0.103	0.540±0.034	0.450±0.085
6	0.567±0.074	0.636±0.033	0.518±0.096	0.512±0.037	0.452±0.078

Fig.5 Colony morphological characteristics after dual cultures of Pleurotus pulmonarius and pathogen for 5 d Photos from left to right show the results of CK (without pathogen inoculation) and treatments after inoculation of CAJ01-D, TXX01, CAJ06-C, LAD07, respectively.

Fig.6 Volcano map of differential expressed genes under treatments -lg(FDR), The opposite number of common logarithm value of false discovery rate. log2(FC), The logarithm value of fold change based on 2.

Fig.7 Venn diagram of differential expressed genes under treatments

Fig.8 Cluster analysis diagram of GO function

References 28

[1]	戴玉成, 周丽伟, 杨祝良, 等. 中国食用菌名录[J]. 菌物学报, 2010, 29(1): 1-21.
	DAI Y C, ZHOU L W, YANG Z L, et al. A revised checklist of edible fungi in China[J]. Mycosystema, 2010, 29(1): 1-21. (in Chinese with English abstract)
[2]	张金霞, 黄晨阳, 郑素月. 平菇新品种: 秀珍菇的特征特性[J]. 中国食用菌, 2005, 24(4): 26.
	ZHANG J X, HUANG C Y, ZHENG S Y. Characteristics of a new variety of oyster mushroom: Pleurotus pulmonarius[J]. Edible Fungi of China, 2005, 24(4): 26. (in Chinese)
[3]	宗亭轩, 陈青, 陆中华. 2021年浙江省食用菌产业产销特点回溯及相关建议[J]. 食药用菌, 2022, 30(6): 393-398.
	ZONG T X, CHEN Q, LU Z H. Analysis of production and marketing characteristics and development suggestions of edible mushroom in Zhejiang Province in 2021[J]. Edible and Medicinal Mushrooms, 2022, 30(6): 393-398. (in Chinese with English abstract)
[4]	周振辉, 廖浩锋, 陈多扬, 等. 食用菌工厂化栽培中病虫害防控措施浅析[J]. 中国食用菌, 2019, 38(5): 52-56.
	ZHOU Z H, LIAO H F, CHEN D Y, et al. Analysis on disease and pest control in mushroom factory production[J]. Edible Fungi of China, 2019, 38(5): 52-56. (in Chinese with English abstract)
[5]	秦文韬, 王守现, 荣成博, 等. 我国食用菌病害发生与防控概况[J]. 中国食用菌, 2020, 39(12): 1-7.
	QIN W T, WANG S X, RONG C B, et al. Occurrence and management of edible fungus diseases in China[J]. Edible Fungi of China, 2020, 39(12): 1-7. (in Chinese with English abstract)
[6]	郭倩, 周昌艳, 谭琦, 等. 华东地区秀珍菇黄菇病的发生及防治[J]. 中国食用菌, 2004, 23(1): 30-31.
	GUO Q, ZHOU C Y, TAN Q, et al. Occurrence and control of Pleurotus eryngii yellow mushroom disease in East China[J]. Edible Fungi of China, 2004, 23(1): 30-31. (in Chinese)
[7]	袁卫东, 陆娜, 宋吉玲, 等. 浙江秀珍菇黄菇病病原菌的分离与鉴定[J]. 浙江农业学报, 2018, 30(11): 1893-1898.
	YUAN W D, LU N, SONG J L, et al. Pathogen isolation and identification of yellow rot disease in Pleurotus geesteranus in Zhejiang Province[J]. Acta Agriculturae Zhejiangensis, 2018, 30(11): 1893-1898. (in Chinese with English abstract)
[8]	LU N, YUAN W D, WU Y L, et al. Identification of contaminants as members of the Bacillus pumilus group and analysis of contamination sources in the cultivation of Pleurotus geesteranus mushrooms[J]. Journal of Plant Pathology, 2021, 103(1): 105-115.
[9]	陆娜, 闫静, 王伟科. 秀珍菇枯萎病的病原菌鉴定及污染源分析[J]. 食用菌学报, 2023, 30(2): 92-101.
	LU N, YAN J, WANG W K. Identification and pollution source analysis of Pleurotus pulmonarius blight disease caused by Ewingella americana[J]. Acta Edulis Fungi, 2023, 30(2): 92-101. (in Chinese with English abstract)
[10]	崔凯, 吴伟伟, 刁其玉. 转录组测序技术的研究和应用进展[J]. 生物技术通报, 2019, 35(7): 1-9.
	CUI K, WU W W, DIAO Q Y. Application and research progress on transcriptomics[J]. Biotechnology Bulletin, 2019, 35(7): 1-9. (in Chinese with English abstract)
[11]	赵慧. 平菇黄斑病病原菌的分离鉴定、侵染机制与生物防治研究[D]. 太谷: 山西农业大学, 2019.
	ZHAO H. Isolation, identification, infection mechanism and biological control of the pathogen causing yellow blotch disease in Pleurotus ostreatus[D]. Taigu: Shanxi Agricultural University, 2019. (in Chinese with English abstract)
[12]	王青. 金针菇两种细菌性病害鉴定及其响应病原菌侵染的转录组分析[D]. 武汉: 华中农业大学, 2020.
	WANG Q. Identification of two bacteriosis of Flamulina filiformis and transcriptome analysis of response to the pathogen infection[D]. Wuhan: Huazhong Agricultural University, 2020. (in Chinese with English abstract)
[13]	远方, 屈淑平, 崔崇士, 等. 一株新的胡萝卜软腐欧文氏菌的分离和鉴定[J]. 微生物学报, 2004, 44(2): 136-140.
	YUAN F, QU S P, CUI C S, et al. A new strain of Erwinia carotovora subsp. carotovora isolated from soft-rotted Chinese cabbage[J]. Acta Microbiologica Sinica, 2004, 44(2): 136-140. (in Chinese with English abstract)
[14]	张琦, 王艳飞, 庞圆圆, 等. 显微镜检测中微生物的革兰氏染色观察鉴定[J]. 中国食品工业, 2013(7): 59-60.
	ZHANG Q, WANG Y F, PANG Y Y, et al. Observation and identification of microorganisms in microscope detection by Gram staining[J]. China Food Industry, 2013(7): 59-60. (in Chinese)
[15]	范华, 董宽虎, 方志红, 等. 碱蒿营养器官的扫描电镜观察研究[J]. 激光生物学报, 2010, 19(3): 314-320.
	FAN H, DONG K H, FANG Z H, et al. Studies on microscopic structure of nutritive organs in Artemisia anethifolia[J]. Acta Laser Biology Sinica, 2010, 19(3): 314-320. (in Chinese with English abstract)
[16]	LANE D J. 16S/23S rRNA sequencing[M]// STACKEBRANDTE, GOODFELLOWM. Nucleic acid techniques in bacterial systematic. New York: John Wiley and Sons, 1991: 115-175.
[17]	YOUNG M D, WAKEFIELD M J, SMYTH G K, et al. Gene ontology analysis for RNA-seq: accounting for selection bias[J]. Genome Biology, 2010, 11(2): R14.
[18]	MAO X Z, CAI T, OLYARCHUK J G, et al. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary[J]. Bioinformatics, 2005, 21(19): 3787-3793.
[19]	陆中华. 浙江省食用菌产业生产现状与发展思考[J]. 中国食用菌, 2023, 42(4): 110-114.
	LU Z H. The status and development suggestions of edible fungi industry in Zhejiang Province[J]. Edible Fungi of China, 2023, 42(4): 110-114. (in Chinese with English abstract)
[20]	汪彩云, 黄启树, 钱海燕, 等. 秀珍菇黄菇病的调查与防治建议[J]. 蔬菜, 2010(1): 26-27.
	WANG C Y, HUANG Q S, QIAN H Y, et al. Investigation and control suggestions on yellow mushroom disease of Pleurotus pulmonarius[J]. Vegetables, 2010(1): 26-27. (in Chinese)
[21]	边银丙, 徐璟煜. 草腐食用菌病害无公害防治技术对策初探[J]. 食用菌, 2008, 30(6): 55-56.
	BIAN Y B, XU J Y. Preliminary study on pollution-free control measures of grass-rotting edible fungi diseases[J]. Edible Fungi, 2008, 30(6): 55-56. (in Chinese)
[22]	SARASTE M. Oxidative phosphorylation at the fin de siècle[J]. Science, 1999, 283(5407): 1488-1493.
[23]	RICH P R, MARÉCHAL A. The mitochondrial respiratory chain[J]. Essays in Biochemistry, 2010, 47: 1-23.
[24]	WALLACE D C. A mitochondrial paradigm of metabolic and degenerative diseases, aging, and cancer: a dawn for evolutionary medicine[J]. Annual Review of Genetics, 2005, 39: 359-407.
[25]	NAVARRO A, BOVERIS A. The mitochondrial energy transduction system and the aging process[J]. American Journal of Physiology Cell Physiology, 2007, 292(2): C670-C686.
[26]	WANG Q, XU R P, GUO M P, et al. Serial transcriptional changes of Flammulina filiformis(winter mushroom) mycelia infected by Pseudomonas migulae[J]. Scientia Horticulturae, 2022, 297: 110965.
[27]	YANG X M, YANG K X, WANG X H, et al. Transcriptomic analysis reveals the mechanism of bacterial disease resistance of postharvest button mushroom (Agaricus bisporus)[J]. Physiological and Molecular Plant Pathology, 2022, 122: 101903.
[28]	YAN D, GAO Q, RONG C B, et al. Comparative transcriptome analysis of abnormal cap and healthy fruiting bodies of the edible mushroom Lentinula edodes[J]. Fungal Genetics and Biology, 2021, 156: 103614.

Identification of yellow rot disease of Pleurotus pulmonarius and analysis of its pathogenicity-related transcriptome

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