Abstract: F₁ and F₂ progeny of cross combination with TYLCVD-resistant tomato material T0727 and susceptible material T9179 were inoculated. With 64 EcoRⅠ/MseⅠ primer combinations, AFLP analysis was performed on two parents, their F₁ and F₂ resistant and susceptible bulks. A total of about 4 023 distinguishable bands were amplified, three of which were stable. Genetic linkage analysis of the three polymorphic DNA fragments with the resistance genes was tested in the F₂ segregating population derived from the cross with T0727 and T9179. The DNA fragment E-ACC/M-CAG was found to be closely linked to one of the TYLCVD-resistant genes, with a genetic distance of 9.5 cM, which was tentatively named Afty-196. The cloned fragment E-ACC/M-CAG was sequenced and then successfully converted to a SCAR marker, which can be used in marker-assisted selection for tomato resistant to tomato yellow leaf curl virus disease．

Key words: tomato, Tomato yellow leaf curl virus disease (TYLCVD), AFLP, SCAR