Identification of the pathogen causing mulberry snags rotten leaves disease and resistance of 47 mulberry varieties

doi:10.3969/j.issn.1004-1524.20240999

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (2): 301-309.DOI: 10.3969/j.issn.1004-1524.20240999

• Plant Protection • Previous Articles Next Articles

Identification of the pathogen causing mulberry snags rotten leaves disease and resistance of 47 mulberry varieties

CHEN Quan¹^,²(), LI Hui¹^,², YANG Jing¹^,², XU Songlin¹^,², ZHANG Shukang¹^,², LIU Zhongxian²^,³^,^*(), HE Jinhui³^,^*()

1. Chongqing Three Gorges Academy of Agricultural Sciences, Chongqing 404155, China
2. College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing 404100, China
3. Economic Crop Development Center in Wanzhou District of Chongqing City, Chongqing 404401, China

Received:2024-11-21 Online:2026-02-25 Published:2026-03-24

Abstract

Abstract:

To identify the pathogen responsible for the prevalent leaf diseases in the mulberry production area of Qianjiang, Chongqing, and to evaluate the resistance levels of different mulberry varieties for establishing precise and efficient control methods to reduce mulberry leaf loss, this study was conducted. Through field sampling, indoor isolation and cultivation, PCR amplification of ITS, ACT, TUB, and TEF gene fragments, and construction of a phylogenetic tree, the pathogen was identified. The resistance of 47 mulberry varieties was evaluated by in vitro leaf inoculation and assessment of disease incidence and average diameter of disease spots after 6 days. The strain HBB1, isolated from mulberry leaves in Qianjiang District, Chongqing, was identified as a new variant of the mulberry snags rotten leaves disease (Boeremia exigua), named Boeremia exigua var. mori. After inoculation with HBB1, the incidence rate reached 100% for 21 varieties including Chuansang 7657, Husang 32, and Nongsang 14, while Chuansang 90-4 showed the lowest incidence rate (53.84%). Regarding disease spot diameter, 6 varieties including Chuan Sang 33, Chuan Sang 39, and Chuan Sang 7657 exhibited disease spot diameters greater than 12 mm, and were classified as highly susceptible varieties. In contrast, Fushe 1 showed a disease spot diameter of 2.09 mm, identifying it as a highly resistant variety. Additionally, 32 varieties demonstrated resistance or moderate resistance. In conclusion, this study is the first to confirm that the pathogen causing mulberry snags rotten leaves disease in Qianjiang, Chongqing, is the new variety Boeremia exigua var. mori. It systematically reveals the differences in resistance among mulberry varieties to this pathogen, providing a scientific basis for breeding resistant varieties and implementing precise disease control.

Key words: mulberry, snags rotten leaves disease, Boeremia exigua var. mori, pathogen identification, resistance evaluation

CLC Number:

S888.7

CHEN Quan, LI Hui, YANG Jing, XU Songlin, ZHANG Shukang, LIU Zhongxian, HE Jinhui. Identification of the pathogen causing mulberry snags rotten leaves disease and resistance of 47 mulberry varieties[J]. Acta Agriculturae Zhejiangensis, 2026, 38(2): 301-309.

Figures/Tables 8

References 26

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扩增区域 Amplified region	正向引物序列(5'→3') Forward primer sequence (5'→3')	反向引物序列(5'→3') Reverse primer sequence (5'→3')
ITS	TCCGTAGGTGAACCTTGCGG	TCCTCCGCTTATTGATATGC
ACT	ATGTGCAAGGCCGGTTTCGC	TACGAGTCCTTCTGGCCCAT
TUB	GGTAACCAAATCGGTGCTGCTTTC	AACCTCAGTGTAGTGACCCTTGGC
TEF	CATCGAGAAGTTCGAGAAGG	TACTTGAAGGAACCCTTACC

扩增区域 Amplified region	正向引物序列(5'→3') Forward primer sequence (5'→3')	反向引物序列(5'→3') Reverse primer sequence (5'→3')
ITS	TCCGTAGGTGAACCTTGCGG	TCCTCCGCTTATTGATATGC
ACT	ATGTGCAAGGCCGGTTTCGC	TACGAGTCCTTCTGGCCCAT
TUB	GGTAACCAAATCGGTGCTGCTTTC	AACCTCAGTGTAGTGACCCTTGGC
TEF	CATCGAGAAGTTCGAGAAGG	TACTTGAAGGAACCCTTACC

序号 Code	品种 Cultivar	序号 Code	品种 Cultivar	序号 Code	品种 Cultivar
1	农桑14号Nongsang 14	17	嘉陵20号Jialing 20	33	川桑33 Chuansang 33
2	油桑Yousang	18	嘉陵16号Jialing 16	34	川桑90-4 Chuansang 90-4
3	桂优62 Guiyou 62	19	桐桑Tongsang	35	川桑826 Chuansang 826
4	桂优12 Guiyou 12	20	821 821	36	川桑7657 Chuansang 7657
5	云桑1号Yunsang 1	21	实钴11-6 Shigu 11-6	37	川桑3723 Chuansang 3723
6	云桑2号Yunsang 2	22	西农751 Xinong 751	38	选一号Xuan 1
7	湖桑32号Husang 32	23	蓬1号Peng 1	39	丰田5号Fengtian 5
8	秋茗Qiuming	24	蓬2号Peng 2	40	蔬菜桑Shucaisang
9	农桑8号Nongsang 8	25	小冠桑Xiaoguansang	41	青皮湖桑Qingpihusang
10	双头荷叶白Shuangtouheyebai	26	辅射1号Fushe 1	42	湖桑7号Husang 7
11	荷叶白Heyebai	27	强桑1号Qiangsang 1	43	充场Chongchang
12	北场1号Beichang 1	28	茶叶桑Chayesang	44	南1号Nan 1
13	西农1号Xinong 1	29	川桑48-3 Chuansang 48-3	45	陕桑305 Shansang 305
14	育711 Yu 711	30	川桑98-1 Chuansang 98-1	46	渝桑101 Yusang 101
15	云11 Yun 11	31	台桑14-1 Taisang 14-1	47	饲料桑Siliaosang
16	新之一濑Xinzhiyilai	32	川桑39 Chuansang 39

序号 Code	品种 Cultivar	序号 Code	品种 Cultivar	序号 Code	品种 Cultivar
1	农桑14号Nongsang 14	17	嘉陵20号Jialing 20	33	川桑33 Chuansang 33
2	油桑Yousang	18	嘉陵16号Jialing 16	34	川桑90-4 Chuansang 90-4
3	桂优62 Guiyou 62	19	桐桑Tongsang	35	川桑826 Chuansang 826
4	桂优12 Guiyou 12	20	821 821	36	川桑7657 Chuansang 7657
5	云桑1号Yunsang 1	21	实钴11-6 Shigu 11-6	37	川桑3723 Chuansang 3723
6	云桑2号Yunsang 2	22	西农751 Xinong 751	38	选一号Xuan 1
7	湖桑32号Husang 32	23	蓬1号Peng 1	39	丰田5号Fengtian 5
8	秋茗Qiuming	24	蓬2号Peng 2	40	蔬菜桑Shucaisang
9	农桑8号Nongsang 8	25	小冠桑Xiaoguansang	41	青皮湖桑Qingpihusang
10	双头荷叶白Shuangtouheyebai	26	辅射1号Fushe 1	42	湖桑7号Husang 7
11	荷叶白Heyebai	27	强桑1号Qiangsang 1	43	充场Chongchang
12	北场1号Beichang 1	28	茶叶桑Chayesang	44	南1号Nan 1
13	西农1号Xinong 1	29	川桑48-3 Chuansang 48-3	45	陕桑305 Shansang 305
14	育711 Yu 711	30	川桑98-1 Chuansang 98-1	46	渝桑101 Yusang 101
15	云11 Yun 11	31	台桑14-1 Taisang 14-1	47	饲料桑Siliaosang
16	新之一濑Xinzhiyilai	32	川桑39 Chuansang 39

品种 Variety	发病率/% Incidence/%	病斑直径/mm Diameter of infection sites/mm	抗性 Resistance	品种 Variety	发病率/% Incidence/%	病斑直径/mm Diameter of infection sites/mm	抗性 Resis- tance
川桑33 Chuansang 33	88.89±8.39	12.80±0.26	HS	饲料桑Siliaosang	82.50±10.9	8.28±0.24	MR
川桑39 Chuansang 39	61.37±10.28	13.44±0.41	HS	双头荷叶白Shuangtouheyebai	100.00±0	7.52±0.50	MR
川桑7657 Chuansang 7657	100.00±0	12.40±0.36	HS	云桑2号Yunsang 2	100.00±0	8.50±0.50	MR
川桑48-3 Chuansang 48-3	89.26±8.91	18.42±0.38	HS	南1号Nan 1	90.67±9.29	6.58±0.28	MR
川桑3723 Chuansang 3723	88.79±8.25	17.83±0.24	HS	丰田5号Fengtian 5	100.00±0	6.24±0.25	MR
台桑14-1 Taisang 14-1	80.61±10.05	13.99±0.19	HS	新之一濑Xinzhiyilai	100.00±0	6.83±0.28	MR
川桑826 Chuansang 826	81.83±7.90	10.11±0.37	S	湖桑32 Husang 32	100.00±0	8.23±0.25	MR
川桑90-4 Chuansang 90-4	53.84±12.71	10.02±0.48	S	茶叶桑Chayesang	98.67±2.31	5.74±0.25	R
选一号Xuan 1	88.64±8.05	11.42±0.38	S	821 821	88.61±8.01	5.33±0.28	R
充场Chongchang	100.00±0	9.54±0.34	S	实钴11-6 Shigu 11-6	100.00±0	5.25±0.25	R
云桑1号Yunsang 1	100.00±0	9.98±0.53	S	西农751 Xinong 751	100.00±0	5.30±0.30	R
荷叶白Heyebai	88.61±8.01	10.74±0.25	S	贵优62 Guiyou 62	71.94±12.54	5.38±0.34	R
北场1号Beichang 1	100.00±0	9.56±0.22	S	嘉陵16 Jialing 16	100.00±0	4.76±0.25	R
强桑1号Qiangsang 1	84.89±6.83	9.57±0.21	S	云11 Yun 11	100.00±0	5.39±0.36	R
西农1号Xinong 1	100.00±0	7.11±0.36	MR	嘉陵20 Jialing 20	71.39±10.08	5.04±0.26	R
蔬菜桑Shucaisang	85.56±5.85	7.27±0.25	MR	蓬1号Peng 1	85.89±5.83	5.63±0.35	R
农桑14号Nongsang 14	100.00±0	6.59±0.37	MR	油桑Yousang	78.99±8.52	5.28±0.25	R
陕桑305 Shansang 305	87.94±7.92	7.29±0.26	MR	青皮湖桑Qingpihusang	78.06±7.56	3.58±0.19	R
秋茗Qiuming	100.00±0	8.81±0.27	MR	湖桑7号Husang 7	79.17±8.78	4.76±0.25	R
渝桑101 Yusang 101	100.00±0	7.28±0.25	MR	桐桑Tongsang	100.00±0	5.80±0.26	R
农桑8号Nongsang 8	100.00±0	6.24±0.25	MR	贵优12 Guiyou 12	69.72±9.59	4.53±0.50	R
蓬2号Peng 2	100.00±0	6.79±0.21	MR	小冠桑Xiaoguansang	100.00±0	3.92±0.19	R
育711 Yu 711	83.89±8.39	6.55±0.43	MR	辐射1号Fushe 1	88.55±7.93	2.09±0.52	HR
川桑98-1 Chuansang 98-1	62.22±10.72	6.61±0.26	MR

Identification of the pathogen causing mulberry snags rotten leaves disease and resistance of 47 mulberry varieties

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