Effects of fermented feed on the growth performance and intestinal bacterial flora of juvenile Litopenaeus vannamei

doi:10.3969/j.issn.1004-1524.20250442

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (8): 1539-1550.DOI: 10.3969/j.issn.1004-1524.20250442

• Animal Science • Previous Articles Next Articles

Effects of fermented feed on the growth performance and intestinal bacterial flora of juvenile Litopenaeus vannamei

CHEN Jinfei(), LI Pengquan, CHEN Chen^*(), LI Tengteng, JI Dewei, QI Piaopiao, ZHOU Qiang

Zhejiang Key Laboratory of Coastal Biological Germplasm Resources Conservation and Utilization, Sino-Thai Joint Laboratory of Marine Science and Technology, Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Zhejiang Mariculture Research Institute, Wenzhou 325000, Zhejiang, China

Received:2025-06-17 Online:2026-08-25 Published:2026-09-14

Abstract

Abstract:

To investigate the effect of probiotic fermented feed on the nursery performance of juvenile Litopenaeus vannamei, four experimental groups were established, fed with KB (basic feed), RS (feed fermented by Lactobacillus acidophilus), YB (feed fermented by Bacillus subtilis), and YR (feed fermented by compound probiotics), respectively. Juvenile Litopenaeus vannamei with uniform size (0.41±0.01)g were selected for a 32-day nursery test to evaluate the growth performance of the juvenile shrimp, and microbial composition in the aquaculture water and shrimp intestines. The results showed that the fermented feed groups significantly(p<0.05) increased the survival rate of the juvenile shrimp, and significantly (p<0.05) reduced the feed conversion ratio; in the aquaculture water of the fermented feed groups, the relative abundance of Xanthomonadales significantly(p<0.05) increased, while the relative abundances of Rhizobiales, Parvibaculales, and Vibrionales significantly (p<0.05) decreased. The results of this study indicated that although fermented feeds exerted no significant effect on the overall microbial community structure of aquaculture water, they reduced the relative abundance of Vibrionales, improved the survival rate of juvenile Litopenaeus vannamei, optimized the composition of their intestinal flora, and thereby enhanced feed utilization efficiency and growth rate.

Key words: fermented feed, juvenile Litopenaeus vannamei, juvenile shrimp, growth performance, bacterial community diversity, flora composition

CLC Number:

CHEN Jinfei, LI Pengquan, CHEN Chen, LI Tengteng, JI Dewei, QI Piaopiao, ZHOU Qiang. Effects of fermented feed on the growth performance and intestinal bacterial flora of juvenile Litopenaeus vannamei[J]. Acta Agriculturae Zhejiangensis, 2026, 38(8): 1539-1550.

Figures/Tables 10

References 25

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试验组 Experimental group	菌种 Strain	含量Content
试验组 Experimental group	菌种 Strain	发酵前/10⁴ Before fermentation /10⁴	发酵后/10⁷ After fermentation /10⁷
YB	枯草芽孢杆菌Bacillus subtilis	7.54±1.14	7.65±0.95
RS	嗜酸乳杆菌Lactobacillus acidophilus	4.25±0.61	6.81±0.81
YR	复合益生菌Compound probiotics	8.99±1.16	56.62±6.10
KB	—	0	0

试验组 Experimental group	菌种 Strain	含量Content
试验组 Experimental group	菌种 Strain	发酵前/10⁴ Before fermentation /10⁴	发酵后/10⁷ After fermentation /10⁷
YB	枯草芽孢杆菌Bacillus subtilis	7.54±1.14	7.65±0.95
RS	嗜酸乳杆菌Lactobacillus acidophilus	4.25±0.61	6.81±0.81
YR	复合益生菌Compound probiotics	8.99±1.16	56.62±6.10
KB	—	0	0

试验组 Experimental group	终体重/g Final body weight /g	增重率/% Weight gain rate/%	成活率/% Survival rate/%	特定生长率/% Specific growth rate/%	饲料系数 Feed conversion ratio
RS	1.062±0.045 a	157.55±11.29 a	92.39±3.00 a	2.95±0.14 a	3.56±0.45 c
YB	1.043±0.038 a	152.72±9.49 a	89.36±9.17 a	2.90±0.11 a	3.94±0.56 bc
YR	1.041±0.030 a	152.41±7.18 a	84.72±9.91 a	2.89±0.11 a	4.53±1.22 b
KB	0.971±0.100 a	135.42±24.63 a	78.69±14.36 b	2.66±0.33 a	6.24±2.25 a

试验组 Experimental group	终体重/g Final body weight /g	增重率/% Weight gain rate/%	成活率/% Survival rate/%	特定生长率/% Specific growth rate/%	饲料系数 Feed conversion ratio
RS	1.062±0.045 a	157.55±11.29 a	92.39±3.00 a	2.95±0.14 a	3.56±0.45 c
YB	1.043±0.038 a	152.72±9.49 a	89.36±9.17 a	2.90±0.11 a	3.94±0.56 bc
YR	1.041±0.030 a	152.41±7.18 a	84.72±9.91 a	2.89±0.11 a	4.53±1.22 b
KB	0.971±0.100 a	135.42±24.63 a	78.69±14.36 b	2.66±0.33 a	6.24±2.25 a

试验组 Experimental group	细菌总数Total number of bacteria
试验组 Experimental group	3 d	6 d	12 d	19 d	25 d	32 d
RS	3 950±526 b	3 545±304 c	57 750±7 632 ab	39 375±4 715 b	58 100±8 102 b	46 100±5 553 a
YB	7 625±822 a	5 750±2 253 bc	55 500±10 630 ab	27 000±5 492 b	56 000±5 354 b	51 725±9 397 a
YR	7 250±835 a	7 275±2 634 ab	46 750±13 175 b	24 250±2 872 b	45 575±11 189 b	52 250±3 594 a
KB	3 000±424 c	8 575±1 069 a	70 500±8 544 a	657 500±86 554 a	160 500±29 558 a	50 500±7 234 a
SLS	210±15 d	530±15 d	570±70 c	365±35 c	1 140±60 c	620±40 b

Effects of fermented feed on the growth performance and intestinal bacterial flora of juvenile Litopenaeus vannamei

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样本 Sample	ACE指数 ACE index	Chao1指数 Chao1 index	香农指数 Shannon index	辛普森指数 Simpson index	覆盖度/% Coverage/%
RS	258.8±80.9 a	256.4±78.5 a	3.5±0.3 a	0.10±0.01 a	99.95±0.02 a
YB	176.0±35.0 a	175.6±34.4 a	3.2±0.4 a	0.10±0.01 a	99.98±0.01 a
YR	198.9±12.0 a	199.0±12.6 a	3.3±0.4 a	0.10±0.10 a	99.98±0.02 a
KB	183.9±84.7 a	182.9±83.7 a	2.9±0.9 a	0.20±0.20 a	99.98±0.02 a

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