Abstract: Using Penaeus vannamei, Metapenaeus ensis and Macrobrachium rosenbergii as materials, the shrimp meat electrophoretic patterns with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE) which showed high resolution and good repetition was established. The comparison study was conducted to analyze the differentially expressed proteins among three breeds. The result indicated that 37 spots showed a significant difference among three shrimp species by using statistical and quantity analysis. The 2-DE maps of Penaeus vannamei, Metapenaeus ensis and Macrobrachium rosenbergii meats demonstrated 15,3,7 spots respectively as characteristic protein spots．

Key words: two-dimensional gel electrophoresis, shrimps, differential proteomics, meat quality