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    25 December 2015, Volume 27 Issue 12 Previous Issue    Next Issue

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    Effects of growing rice roots on the bacterial abundance and community structure in the rhizosphere during tillering stage
    ZHANG Zhen\|xing1,2, ZHANG Wen\|zhao1, YANG Hui\|cui1,2, CHEN Chun\|lan1, WEI Wen\|xue1,*
    2015, 27(12):  2045. 
    Abstract ( 423 )   PDF (2608KB) ( 1467 )  
    In the pot experiment, bacterial 16S rRNA gene was targeted as the molecular biomarker. Quantitative PCR (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were applied to explore the effects of rice roots on the bacterial community during the rice growing season. Results showed that bacterial abundance in the rhizospheric soil was significantly increased by rice roots, but Shannon diversity index (H′) and evenness index (E) of bacterial community were lower in the rhizosphere than bulk soil. Bacterial community structures were clearly different between rhizosphere and non-rhizosphere soil, dominant bacterial communities appeared with a relatively higher abundance in the rhizosphere soil, while the dominant bacterial communities were different between the flooded and drained treatments. Soil properties in the rhizosphere soil were obviously influenced by the rice roots. Bacterial community structures were closely correlated with soil NH+4-N, TC under the flooded treatment, but with soil pH and moisture under the drained. In summary, growing rice roots could exert significant influence on bacterial abundance and community structures during rice growing season, which could be induced by the variation of soil properties due to rice roots activities. Therefore, rice roots and water status might be important factors affecting bacterial ecological function in paddy fields.
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    Detection of genetic stability in the regenerated plantlets of Jiangxi Yanshan red bud taro (Colocasia esculenta L. Schott var. cormosus cv. Hongyayu) using RAPD analysis and flow cytometry
    YIN Ming\|hua, HONG Sen\|rong*, WANG Ai\|ping, LIN Guo\|wei, WANG Ai\|bin, KE Wei\|zhong
    2015, 27(12):  2053. 
    Abstract ( 400 )   PDF (3001KB) ( 1285 )  
     The RAPD analysis and flow cytometry detection of genetic stability of Jiangxi Yanshan red bud taro regenerated plantlets were studied, which provide a theoretical basis for conservation in vitro of its germplasm resource and factory production of plantlets. The experimental results showed that, the fluorescence intensity value of the first and the second peak of 9 kinds of regenerated plantlets and the control group of Jiangxi Yanshan red bud taro had no significant change by flow cytometry detection. DNA of 9 kinds of regenerated plantlets and the control group of Jiangxi Yanshan red bud taro were amplified by RAPD using 20 random primers. The amplified fragment size was 200-3 000 bp. The amplification number of 20 primers was 12, 10, 7, 8, 9, 14, 9, 14, 10, 8, 10, 10, 10, 10, 11, 8, 9, 10, 8, 15. 202 site(strip) were obtained, each primer produced 10.1 site, of which polymorphic loci was 160, the average number detected by each primer was 8 and the average percentage of polymorphic loci was 79.21%. Using NTsys210 software, clustering analysis of 9 kinds of regenerated plantlets and the control group of Jiangxi Yanshan red bud taro was studied, which were divided into two categories, one category had 1 kind, namely callus regenerated plantlets, another category included 8 kinds of regenerated plantlets and the control group. The results also showed that callus regenerated plantlets had significant differences compared with other 8 kinds of regenerated plantlets and the control group, while there was no difference between the other 8 regeneration plantlets and the control group.
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    The chemical mutation breeding of a new type of sweet potato Yongzishu No.1
    WANG Fang1, WEI Zhang\|huan2, LU Xing\|miao2,CHENG Ye3,WANG Yi\|ting3, HUANG Jian1,CHEN Zhi3, YAN Cheng\|qi1,3,*
    2015, 27(12):  2061. 
    Abstract ( 383 )   PDF (648KB) ( 1300 )  
    In this study, we used chemical mutation breeding method to breed a new variety of purple sweet potato. We adopted 0, 0.5%, 1.0%, 1.5% of sodiumazide to treat with embryogenic cell masses for 6 hours by using parental Australian purple sweet potato of Au1990sp. The result showed that the treatment of 0.5% sodiumazide for 6 h was the best. After redifferentiation, the embryogenic callus in the treatment of 0.5% sodiumazide for 6 h were differentiated into 125 strains, and 99 strains were obtained after transplantation in 2003. Among the 14 strains selected in 2003, 3 strains obtained high yield, indicating 43.98 t·hm-2 of CA06, 40.03 t·hm-2 of CA11 and 43.84 t·hm-2 of CA17. The yield of the control group of Au1990sp was only 4.95 t·hm-2. Moreover, the mutated strains showed quite a lot of difference in phenotype, characteristics and features from parental strains. There were obvious differences between the parental strain of Au1990sp and the mutated strain by AFLP analysis. This new variety was named Yongzishu No.1 after 12 years breeding, which was approved to be a new non\|crop variety in December in 2012 in Zhejiang Province.
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    Effects of ionic liquid on the growth, physiological and biochemical characteristics of maize seedlings
    LIU Ping, ZHANG Yan, DING Yi\|feng, LYU Bao\|he, FAN Qi\|qi, WANG Tian\|le, WANG Xue\|rui, LU Fang
    2015, 27(12):  2065. 
    Abstract ( 470 )   PDF (1131KB) ( 1280 )  
    Investigating the toxic effects of ionic liquids on crops could provide basis for the effects of ionic liquid on ecological environment. Effects of 1\|hexyl\|3\|methyl imidazolium bromide ionic liquid(1\|hexyl\|3\|methyl imidazolium bromide, [C6mim]Br)on the growth, physiological and biochemical characteristics of maize seedlings(Tunyu 118) were evaluated by hydroponics with different concentrations of [C6mim]Br(0, 5, 10, 15 mg·L-1). The results showed that when [C6mim]Br concentration was greater than or equals to 10 mg·L-1, plant height and root length of maize seedling were decreased significantly(P<005), and the content of soluble sugar in the leaves was decreased significantly(P<001). Compared with the normal control, the contents of soluble protein in the leaves was decreased significantly whatever [C6mim]Br concentration was set in this study. The POD activities were significantly lower than that of the control when [C6mim]Br concentration was greater than or equals to 10 mg·L-1. When [C6mim]Br concentration was≥15 mg·L-1, the reducing of superoxide dismutase(SOD) activity and increasing of superoxide anion(O·-2) rate all reached significant level(P<001) compared with the control. It proved that ionic liquid [C6mim]Br was harmful to maize, and with the increase of ionic liquid concentration, the poison increased.
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    Effects of agouti gene mutation and protein structure changes of ASIP on hair colour in colour Rex rabbit
    YANG Cui\|jun1,2, GE Jian3, CHEN Sai\|juan1,4, LIU Ya\|juan1,4, CHEN Bao\|jiang1,4, GU Zi\|lin1,4,*
    2015, 27(12):  2071. 
    Abstract ( 481 )   PDF (1172KB) ( 1386 )  
    In oder to evaluate the ASIP loci in color Rex rabbit, gene polymorphism at ASIP loci and the structure of protein were studied, the evolutionary tree of leporidae was also constructed. The resluts showed that three alleles were found at this loci in the color Rex rabbit, A: light\|bellied agouti(wild type), including brown and chinchilla color; at: tan\|agouti, including tan color; a: non\|agouti, including black, yellow and blue color. Phylogenetic and homologies analysis of the genes at agouti loci from color Rex rabbits and other species of leporidae showed that color Rex rabbits had the highest genetic relationship with Lepus europaeus. Wild type ASIP gene and tan\|agouti gene both encoded a sequence of 133 amino acids with a 24\|amino signal peptide and a cysteine\|rich hydrophobic region at C\|terminal, so this protein had important biological activity. But the differences of these two protein sequences, especially in the conserved regions, caused the change of eumelanin and phaeomelanin, which made the Rex rabbits exhibit different coat color. Non\|agouti gene encoded a sequence of 21 amino acids, it was unstable and could not produce ASIP, so the coat color was black/dark in Rex rabbit.
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    Study on the expression of EF\|hand gene in Hyriopsis cumingii
    QI Xiao\|xiang1, LI Wen\|juan1, SHI Zhi\|yi1,2,*, SHANG Chao1, ZHOU Zi\|rui1, SHANG Pan1
    2015, 27(12):  2078. 
    Abstract ( 316 )   PDF (1809KB) ( 1470 )  
     EF\|hand domain\|containing protein of pearl oyster played an important role in the regulation of calcium metabolism in pearl formation. Strengthening the study of the EF\|hand protein was very important to cultivate large high\|quality pearls. In this study, seven EF\|hand genes were screened from transcriptome of Hyriopsis cumingii, then bioinformatics analysis of them was performed. Expression trends and levels of these seven EF\|hand genes were analyzed at different periods in the mantle and visceral mass after inserted nucleus through the digital gene expression profiling (DGE) and real\|time quantitative PCR. The results showed that these seven EF\|hand genes had classic conservative EF\|hand module. Phylogenetic analysis showed that EFCB1 and EFCB6, EFCB7 and N\|EFCB1, EFCBD2 and RASEFCB had closer representation than the others. DGE analysis showed that EF\|hand genes at different culturing period had different expression levels in mantle and visceral mass organizations of Hyriopsis cumingii, suggesting that these seven genes had different functions during the formation of pearl. Fluorescence quantitative PCR results of these seven genes in mantle and visceral mass at different periods after inserting nucleus showed the same trend as DGE analysis. EFCB1 showed the highest expression level in mantle at 20 days after nucleus inserting (P<0.05), EFCB6 and EFCB7 showed the highest expression level in visceral mass at 20 days after nucleus inserting (P<0.05), suggesting that these 3 genes might play important roles in the initial phase of nacre secretion forming process. This study provided useful information for further study on the regulation of calcium metabolism and pearl formation.
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    Effect of climatic factors change on the populations of honeybee, Apis mellifera L.
    HU Zong\|wen, YANG Juan, WANG Yan\|hui*
    2015, 27(12):  2086. 
    Abstract ( 361 )   PDF (692KB) ( 1299 )  
    In order to investigate the effect of climatic factor change on the populations of honeybee in Caoba area, we recorded the climatic factor change in July, August, September 2014 by meteorograph of Vantage Pro2 and compared the numbers of eggs, larvae, pupae, workers and food storage measured by standard frame every 5 days in the same time. The results showed that there were negative correlations between temperature and larvae (r=-0.29), food storage (r=-0.17), workers (r=-0.65), while the relative humidity had a positive correlation with the colony factors in July. In August, temperature had positive correlation with pupae (r=0.08) and food storage (r=0.73, P<0.05), and had negative correlation with larvae (r=-0.53), workers (r=-0.61); Relative humidity however was quite opposite, which had negative correlation with pupae (r=-0.06), food storage (r=-0.74), and had positive correlation with larvae (r=0.51), worker( r=0.61). Furthermore, in September, temperature had positive correlation with the factors of honeybee population with larvae (r=0.62, P<0.05), pupae (r=0.47, P<0.05), food storage (r=0.7, P<0.05), workers (r=0.79, P<0.01); On the contrary, relative humidity had negative correlation with these factors with pupae (r=-0.21), food storage (r=-0.24), worker (r=-0.06) except for larvae (r=0.15). The climatic factor decided temperature and humidity of macro environment which had relationship with the development of honeybee population. In conclusion, the population dynamics of honeybee (Apis mellifera L.) in temperature regions showed a characteristic seasonal pattern.
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    Effects of mixed ratio and effective microorganism (EM) addition on the mixed silage quality of alfalfa (Medicago sativa L.) and naked oats (Avena nuda)
    GE Jian1, YANG Cui\|jun2, LIU Gui\|he1, *, YANG Zhi\|min3, BAI Xue\|mei2
    2015, 27(12):  2093. 
    Abstract ( 330 )   PDF (760KB) ( 1321 )  
    Nutritional evaluation of mixed ratio and effective microorganism (EM) on mixed silage made of alfalfa (Medicago sativa L.) and naked oats (Avena nuda) in different proportions (1∶0.5, 1∶1 and 1∶2) were carried out in present study. The changes between fermentation quality and nutrition composition were observed after 60 days. The results showed that the fermentation quality of silage was improved gradually with the increase of the proportion of naked oats. The pH value and the ratio of NH3\|N/TN were decline, and the lactic acid (LA) content was increased with the addition of EM. The contents of total volatile fatty acids (TVFAs) of the treatments with the addition of 0.1% EM and 0.2% EM were significantly lower than those of the control group (P<0.05). The most significant effects on reducing the content of TVFAs and increasing the proportion of lactic acid and acetic acid were observed when alfalfa and naked oats were mixed in 1∶2 and with the addition of 0.1% EM. The content of crude protein (CP) was increased, and the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were reduced with the increase of alfalfa proportion in mixed silage. The effects of EM on nutritional ingredient of mixed silage were not significant, and with the addition of 0.1% EM or 0.2% EM in mixed silage which the proportion of naked oats were increased, the consumption of water soluble carbohydrate (WSC) was accelerated, and the fermentation quality of mixed silage was improved. In a word, the mixed silage of alfalfa and naked oats could make nutrition complementary, improve nutrition value, and addition with 0.1% EM or 0.2% EM in mixed silage could help to depress bad fermentation and improve the fermentation quality.
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    Study on heterogeneity of carbon stocks for five forest recovery modes in karst area of Guizhou#br#
    LIAO Xiao\|feng, ZHAO Xiao\|peng, XIE Yuan\|gui*
    2015, 27(12):  2100. 
    Abstract ( 376 )   PDF (517KB) ( 1278 )  
    The heterogeneity of carbon stocks for five forest recovery modes in karst area of Guizhou was studied using methods of quadrat investigation and laboratory analysis, which provided reference for the study on carbon cycle and carbon balance mechanism of plantations. The results showed that: (1) Carbon stocks in different organs of tree were different, carbon stocks of branches in the other four kinds of tree was the lowest except Broussonetia papyrifera, carbon stocks of root in Sophora japonica, Fraxinus chinensis and Broussonetia papyrifera were the highest, while the carbon stocks of trunk in Ligustrum lucidum and Zenia insignis were the highest. (2) Carbon stocks of root in the other four kinds of bushes were the highest except Magnolia multiflora; (3) Carbon stocks in different organs of fujimoto were different. Carbon stocks of branch in mode 1 and mode 2 were the highest, while, carbon stocks of leaf in the other three modes were the highest; (4) Carbon stocks of herbaceous were aboveground >underground in all modes except the control, and the difference was significant.
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    Semi\|quantitative RT\|PCR analysis of cold\|stress induced and differentially expressed genes in fingered citron (Citrus medica var. sarcodactylis Swingle) and Poncirus trifoliata
    ZHANG Zhen\|zhen1, QIU Li\|jun1,LI Ling2, CHEN Wen\|rong2, YING Hai\|liang1, GUO Wei\|dong2,*
    2015, 27(12):  2105. 
    Abstract ( 496 )   PDF (1207KB) ( 1321 )  
    Aimed to investigate cold sensitive genes in fingered citron (Citrus medica var. sarcodactylis Swingle) and unveil the differences of cold resistance mechanism between C.medica and Poncirus trifoliata. The expression patterns of 34 cold\|stress induced genes in C.medica and P.trifoliata were analyzed by semi\|quantitative RT\|PCR after being exposed to -4 ℃ for 24 h. The results showed that the expression levels of 17 genes were different between C.medica and P.trifoliata, among which the transcripts levels of 14 genes changed response to cold\|stress, while no changes were observed in P.trifoliate simultaneously. The transcript levels of 3 genes were found with contrary changing models in two plant species. Besides, the expression of 8 genes in C.medica shared similar pattern as in P.trifoliata, and 9 genes failed to be amplified in P.trifoliata. Thus, it was suggested that the 17 genes differentially expressed in these two plant species were related to cold sensitivity of C.medica. The pathway they were involved in might be the important molecular mechanism regarding cold sensitivity of C.medica. Nine genes with different expression levels induced by cold\|stress in C.medica, which could not be amplified in P.trifoliata, might be the important genes involved in the cold sensibility of C.medica and the important reason resulted in the different cold resistance between C.medica and P.trifoliata.
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    Effects of organic cultivation on fruit quality of Kyoho grape
    LIU Shuang\|shuang1, YU Xin\|guang1, GAO Chang\|da2, FENG Ya\|bin1, QIAN Guang1, LU Si\|yun1, WU Yue\|yan1, WANG Zhong\|hua1,*
    2015, 27(12):  2114. 
    Abstract ( 649 )   PDF (2031KB) ( 1386 )  
    Three year\|old Kyoho grape under organic cultivation and conventional cultivation were used as materials, fruit diameter, fruit weight, total soluble solids (TSS) content, titratable acid (TA) content, TSS/TA and Vitamin C (VC) content in different development periods of grape were determined, free amino acid content and fruit texture in the mature stage were examined. The aim of this research was to explore the effects of organic cultivation on fruit quality in Kyoho grapes. The results indicated that the contents of TSS, TA, TSS/TA, VC and soluble protein in Kyoho grapes fruit under organic cultivation were significantly higher than those of control. Fruit weight and diameter were similar with the control group during the whole development stage, but they were slightly reduced than those of control in the berries mature stage. This study showed that organic cultivation could improve the quality of Kyoho grapes.
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    Effects of antitranspirant on physiological characteristics of Bougainvillea spectabilis under drought stress
    SU Wen\|feng1, SU Zhen\|sheng1, LIN Ling\|ling1, PU\|YANG Xue\|hua2,*
    2015, 27(12):  2122. 
    Abstract ( 548 )   PDF (2543KB) ( 1324 )  
    To investigate the effects of antitranspirant with different concentration on photosynthesis and antioxidant responses of Bougainvillea spectabilis under drought stress, the plants were treated for 21 days under natural drought condition after applying water and 400, 800, 1200 times dilution antitranspirant as a foliar spray, and the plants under normal culture condition were set as the control group. The results showed that antitranspirant reduced the stomatal conductance and transpiration rate, maintained a relatively higher net photosynthetic rate and a stable intercellular CO2 concentration in Bougainvillea spectabilis when compared with single drought stress. Moreover, antitranspirant could alleviate the loss of chlorophyll content and relative water content, reduce the increase of electrolyte leakage and malonaldehyde content, and further improve the activities of SOD, POD, CAT and APX as well as proline content in Bougainvillea spectabilis under drought stress. These results indicated that antitranspirant could enhance the resistance to drought stress of Bougainvillea spectabilis, and the best concentration was the antitranspirant with 800 times dilution.
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    Morphological and physiological responses of sorghum BTx623 seedlings to aluminum toxicity
    HUANG Shou\|chenga, LIU Ai\|ronga, YE Mei\|ronga, CONG Ruo\|lina, ZHAN Qiu\|wenb,*
    2015, 27(12):  2129. 
    Abstract ( 479 )   PDF (1175KB) ( 1296 )  
    The present study investigated the morphological and physiological responses of sorghum BTx623 seedlings under different aluminum (Al) concentrations (0, 25, 50 and 100μmol·L-1, pH=4.5). It was shown that comparing with the control, Al stress significantly reduced the root relative growth (RRG) and root length, while the height of seedlings was not significantly influenced. The fresh weight, dry weight, chlorophyll content and root dehydrogenase activity of seedlings increased by 11.4%, 50.0%, 32.1% and 32.5% under 25 μmol·L-1 Al stress, whereas decreased under 50 and 100 μmol·L-1 Al stresses. Al accumulation, H2O2 production and MDA content of the root notably elevated as the increase of Al concentration. Further analysis revealed that the SOD and CAT activities were significantly up\|regulated by 44.5% and 33.7% under 25 μmol·L-1 Al stress, while POD activity was not significantly affected under Al stress. However, higher concentration of Al (100 μmol·L-1) could inhibit the activities of these three anti\|oxidant enzymes. In conclusion, 25 μmol·L-1 Al stress induced the up\|regulation of anti\|oxidant enzymes to alleviate the oxidative stress and increased the fresh weight, dry weight and chlorophyll content, but inhibited the root elongation of sorghum BTx623 seedlings. However, 50 and 100 μmol·L-1 Al stress had significant inhibitory effects on both above and underground parts of the seedlings.
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    Effects of fulvic acid on the growth and activities of nitrogen metabolism enzymes in pakchoi under NO-3 stress
    PANG Qiang\|qiang, CHEN Ri\|yuan, LIU Hou\|cheng, SONG Shi\|wei, SU Wei, SUN Guang\|wen*
    2015, 27(12):  2136. 
    Abstract ( 418 )   PDF (554KB) ( 1390 )  
     Using leaf spraying method, water culture experiment was carried out for investigating the effects of different concentrations of fulvic acid (0.08%, 0.15% and 0.25%) on the growth and activities of nitrogen metabolism enzymes in pakchoi under NO-3 stress. The results showed that compared with the control, the growth was obviously restrained, photosynthetic pigment contents and activities of nitrogen metabolism enzymes were affected in pakchoi under NO-3 stress. Exogenous fulvic acid treatment could alleviate the damage on growth of pakchoi under NO-3 stress, the contents of photosynthetic pigment and the activities of nitrate reductase, glutamine synthetase, glutamate synthase and glutamate dehydrogenase were increased, contents of nitrate and ammonia were decreased. In conclusion, exogenous fulvic acid treatment could improve the growth and photosynthesis of pakchoi under NO-3 stress, promote plant nitrate uptake and utilization, and further alleviate the damage of pakchoi under NO-3 stress.
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    Remote sensing identification of main fruit tree species based on leaf spectral feature in southern Xinjiang basin
    QI Hao1,2, WANG Zhen\|xi1,2,*, YUE Jun1,2, JIA Yong\|qian1,2
    2015, 27(12):  2141. 
    Abstract ( 525 )   PDF (740KB) ( 1354 )  
    With the rapid development of fruit industry in southern Xinjiang, the classification and extraction of fruit tree has become a hot topic. As the experimental object, this paper selected five kinds of fruit in the southern basin of Xinjiang. In order to find a fast, accurate, large scale, timely, dynamic monitoring of fruit trees and classification method, hyperspectral reflectance data of different leaf species were measured by spectrometer. It would provide necessary technical support to species recognition at the level of spaceborne hyperspectral remote sensing. Based on analysis on the spectral characteristics of five fruit tree species, it employed a way of smoothing filter value treatment under different step sizes and five data transformation methods. The results showed that the species recognition accuracy was the highest after the first derivative transformation, which reached 99.3% under smoothing processing with the step interval of 5 nm. It indicated that this method of remote sensing identification provided a new way for the main tree species in southern Xinjiang.
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    The relationship of bacterial blight incidence and grain loss of single\|cropping rice in the Northwestern Zhejiang
    ZHAO Min1, YAN Cheng\|qi2, HUANG Yuan\|jie1, LI Rong1, ZHANG Guo\|zhong1, ZHENG Chao2, WANG Hua\|di3, CHEN Jian\|ping2,*
    2015, 27(12):  2147. 
    Abstract ( 359 )   PDF (461KB) ( 1326 )  
    The relationship between incidence of rice bacterial blight Xanthomonas campestris and grain loss of single\|cropping rice was analyzed by investigating the incidence level and panicle traits of X.campestris at the same site and date. The results showed that there was no significant difference in panicle length and seed number per ear among different levels of infested plants. However, plant height, filled grain number per panicle, empty flat grain number, filled grain weight, total grain weight per panicle, and 1 000\|seed weigh varied or significantly varied among treatments. The differences between control and 1\|2 level plants were not significant, but the related indicators of 3\|4 level plants were significantly less than those of the control. Filled grain number per panicle, grain weight, total grain weight per panicle and 1 000\|seed weight were decreased by 20.9%-30.8%, 24.3%-36.1%, 20.3%-28.1% and 4.3%-7.6%, respectively, while empty flat grain number per panicle was increased by 132.3%-139.8%. The more serious infested plants, the more significant decrease these parameters. Eleven linear regression equations of disease incidence and grain loss of infested plants at the initial, surge and stable periods of X.campestris were constructed. Such information will be useful in providing basis for forecasting and scientifically controlling X.campestris in the Northwestern Zhejiang.
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    Screening, identification of bio\|control endophytic bacterium against citrus canker and stability of its bioactive metabolites
    LIU Bing, SONG Shui\|lin, LIU Xiao\|li, YANG Ming\|xia, GONG Ling\|ling
    2015, 27(12):  2152. 
    Abstract ( 467 )   PDF (1761KB) ( 1409 )  
    In this experiment, an endophytic bacterium named GN232 against Xanthomonas axonopodis pv. citri Xac. was isolated from navel orange and screened in plate and on leaf in vitro. And the identification and characterisics of its antibacterial active metabolites were studied. By the analyses of morphological, cultural, physiological, biochemical characteristics and 16S rDNA sequence, GN232 was indentified as Bacillus subtilis subsp. subtilis. The activity detection of its fermentation liquid indicated that the antibacterial substances were not sensitive to the temperature less than 100℃, and stable under neutral pH/trypsin, but the activity was decreased under ultraviolet radiation for 25 minutes. In addition, when the fermentation liquid was mixed with agricultural streptomycin, the inhibition effects on Xanthomonas axonopodis pv. citri were increased by 26.8% and 20.7% compared with using fermentation liquid or agricultural streptomycin separately.
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    Quantitative classification, ordination and brief analysis of vegetation communities in retreat area of Hulun Lake
    MA Shuai1,2, FENG Jin\|chao2,*, WU Li\|ji3, LI Yu\|xian2, FENG Ya\|lei2, ZHAO Hui\|qing4
    2015, 27(12):  2159. 
    Abstract ( 296 )   PDF (670KB) ( 1474 )  
    Hulun lake, the largest inland lake in Inner Mongolia, had suffered a 15\|year lake retreat from 1998 to 2012. The lake retreat had formed a 5 km×3 km rectangle shape wetland. The vegetation of the rectangle shape wetland was investigated in mid\|August, 2012. With the help of spatial sequence instead of time successional sequence method, the succession sequence of the sample spot was revealed. After two\|way indicator species analysis (TWINSPAN), 70 sample plots had been divided into 6 groups in Division 5 level. Detrended correspondence analysis(DCA) was also adopted and the successional sequence was revealed as Chenopodiaceae pioneer community (first stage)→ Phragmites australis community (second stage)→ Leymus chinensis community (final stage). After comparing each succession stages biodiversity index (Patrick, Simpson, Shannon\|wiener and Pielou indice) and growth style, it was shown that: (1) The final stage of the succession had the highest biodiversity, evenness and richness; (2) The evenness of the vegetation dropped down a little from the first stage to the second stage, and recovered in the final stage; (3) The proportion of the perennial plant steadily increased from stage one to final stage .
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    Establish and application of the high throughput sequencing method for soil microbial 16S rDNA using Ion Torrent PGM
    MAO Wei\|hua1, WU San\|ling1,ZHANG Xu2
    2015, 27(12):  2165. 
    Abstract ( 484 )   PDF (1083KB) ( 1432 )  
    We aimed to establish the high throughput sequencing method for soil microbial 16S rDNA using Ion Torrent PGM. In this study, the V4\|V5 variable region of 16s rDNA was amplified from the marsh soil and meadow soil by PCR and sequenced respectively. The Shannon curves of the two soil samples tended to be stabilized when the sequencing depth reached 15 000 sequences. The diversity index showed that the bacterial communities in the marsh soil were more abundant than that in the meadow soil. A total of 7 615 and 8 072 OTUs were identified in the marsh soil and meadow soil, respectively, which corresponded to 30 phyla,53 classes,93 orders,130 families and 182 genera in the marsh soil and 29 phyla,52 classes,88 orders,125 families and 183 genera in the meadow soil. The variance analysis showed that there were significant differences in the abundance of microbial compositions and OTUs between the marsh soil and meadow soil. According to the similarity analysis, all the tested samples could be divided into two groups, and the three repeats were classified into the same group. Therefore,it was suggested that the soil microbial 16S rDNA detection method based on Ion Torrent PGM developed in our study could comprehensively and accurately reflect the soil microbial community structure, and provide a powerful tool for the detection of soil microbial community structure.
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    Effect of mushroom residue and pig manure as conditioner on aerobic composting of sewage sludge
    WU Zhen\|zhen, SHU Zeng\|nian, HUANG Jian*
    2015, 27(12):  2171. 
    Abstract ( 342 )   PDF (649KB) ( 1311 )  
    The aerobic composting experiment of sewage sludge with mushroom residue and pig manure as amendments for 40 d was carried out. The dynamics of temperature, water content, pH value, total organic carbon, and total nitrogen were measured during the composting process, and the compost maturity was evaluated by determining the changes of C/N value and germination indexes. It was shown that the mushroom residue and pig manure could be used as amendments for sewage sludge composting. The minimization and harmlessness of sewage sludge could be achieved in short time. The treatment with a proportion of sludge, mushroom residue and straw at 7.0∶2.5∶0.5 by dry weight showed the best performance, and the temperature ran up the fastest and could maintain above 55 ℃ for 6 d. After composting, the germination index of the processed sewage sludge reached 95%, which exhibited the best composting effect.
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    Agricultural ammonia emission inventory and its distribution characteristics in Shanghai
    FANG Xiao\|feng1,2, SHEN Gen\|xiang1,2,*, XU Chang2, QIAN Xiao\|yong2, LI Jin\|wen2, ZHAO Zi\|gang3, YU Shao\|feng3, ZHU Kai\|cheng3
    2015, 27(12):  2177. 
    Abstract ( 422 )   PDF (1292KB) ( 1765 )  
     Based on the activity data of agricultural ammonia emission sources, an agricultural ammonia emission inventory of Shanghai in 2011 was developed by analyzing emission factors in the literature and model. It was shown that the total agricultural NH3 emission in Shanghai was 54.53×103 t in 2011. Livestock and poultry breeding and N fertilizer application were main contributors of agricultural NH3 sources, accounting for 61.2% and 34.3% of total NH3 emission, respectively. Hog was the largest contributor among the livestock and poultry sources, accounting for 56.9% of total livestock and poultry emission, followed by poultry, with a contribution of 34.2%. For spatial distribution, Pudong, Jinshan, Fengxian and Chongming districts were the top four NH3 emissions contributors, which accounted for 66.2% altogether of total NH3 emission in Shanghai. Fengxin was the largest NH3 emission town for livestock and poultry breeding sources, while Liantang Town was the largest contributor for NH3 emission of N fertilizer application. In general, Pudong, Jinshan, Fengxian and Chongming were four key districts for ammonia emission control, while hog, poultry and application of N fertilizer were vital sources for reducing NH3 emissions.
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    Dynamic assessment on water environmental carrying capacity in Taihu Lake Watershed in past 20 years
    CHEN Hai\|tao
    2015, 27(12):  2186. 
    Abstract ( 439 )   PDF (1446KB) ( 1336 )  
    Water environment stress has been increasing in Taihu Lake Watershed due to rapid economic development and population growth. The evaluation index system of water environmental carrying capacity (WECC) in Taihu Lake Watershed was constructed from three aspects of water resource, water pollution and social economy, according to the current situation. The logarithmic function model was selected as a carrying degree model for the single indicator, and the weights of each index contributing to their parent indicators were determined using analytic hierarchy process (AHP). Based on the data from the statistic yearbooks, WECC of Taihu Lake Watershed from 1992 to 2011 was evaluated. The results showed that the key indices in WECC were Engel coefficient of city residents, recycling rate of water used by industries and per capita net income of farmers. The WECC in Taihu Lake Watershed has been increasing during last 20 years, and the status of water environment has changed from fragile in 1992 to normal in 2011. The improvement of water environment might be attributed to the increasing capacity in water pollution subsystem and social economy subsystem. Finally, corresponding policy recommendations to maintain and enhance the WECC of Taihu Lake Watershed were discussed according to the results.
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    The changes of texture in Cyprinidae at low temperature storage and its relationship with freshness
    LIU Li\|rong, CHAI Chun\|xiang*, LU Xiao\|xiang
    2015, 27(12):  2193. 
    Abstract ( 403 )   PDF (594KB) ( 1287 )  
    Rapid detection technology of the freshness of aquatic product was emphasized. The tissue and structure of Cyprinidae changed with its freshness, the changes of Cyprinidae could be indicated by its texture. The texture of Cyprinidae was explored under 4, 0 and -18 ℃, then the relationship between the freshness of Cyprinidae and its texture was analyzed. The results showed that hardness, flexibility and chewiness of Cyprinidae were related to the freshness. A texture model for evaluating the freshness of Cyprinidae was established by using multiple linear regression analysis, the accuracy and verification accuracy of this texture model for determining the freshness was 91.1% and 85.7%, respectively, So this model could be used to predict the freshness of Cyprinidae.
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    Discrimination of Dendrobium officinale and its counterfeits based on naringenin content determination and fingerprints technology
    WANG Shao\|ping1, SUN Yi\|ming2, JIANG Yan2, XIA Zheng\|yan2, WANG Zhi\|an1,2,*
    2015, 27(12):  2199. 
    Abstract ( 352 )   PDF (1322KB) ( 1330 )  
    A new method for the quality evaluation and discrimination of Dendrobium officinale and its counterfeits was developed by means of naringenin content determination combined with fingerprints technology. The differences of chemical compositions were compared between Dendrobium officinale and its counterfeits, which could provide a reference for quality evaluation and identification. HPLC was applied for determination of naringenin in Dendrobium officinale and its counterfeits, and the chromatographic fingerprints were analyzed with similarity evaluation system for chromatographic fingerprint of TCM. DPS was used for cluster analysis and principal component analysis. The results showed that all of the Dendrobium officinales had high similarity and significant differences with the counterfeits. Furthermore, naringenin contents were different among them. In conclusion, the approach established in the present paper offered some technical support for the discrimination of Dendrobium officinale and its counterfeits.
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    Integration and application of touch\|screen fertilization decision\|making system based on ArcGIS runtime for WPF
    MA Wan\|zhu1, LI Li2, LU Ruo\|hui3, ZHU Wei\|feng4, JIANG Yu\|gen4, LYU Xiao\|nan1
    2015, 27(12):  2206. 
    Abstract ( 362 )   PDF (2826KB) ( 1277 )  
    To effectively promote fertilization technology, application of touch\|screen fertilization decision\|making system was developed based on ArcGIS Runtime for WPF. By using the high definition(HD) offline multilevel tile cached map package with existing aerial image tiles and expert knowledge fertilization scheme, the technical threshold for user were degraded, which made the extension of soil formula fertilization technique more feasible.
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    Research on machine vision algorithm for automatic sorting of membrane\|removed mandarin segments
    REN Lei1, ZHANG Jun2,*, LU Sheng\|min2
    2015, 27(12):  2212. 
    Abstract ( 402 )   PDF (1338KB) ( 1430 )  
    During the production of mandarin cans, some membrane\|removed mandarin segments have defects such as membrane residues, seed residues or segment damage, which would affect the quality of products. Currently, factories adopt manual inspection to reject defective segments, which increased the cost and may cause hygiene problems. Based on LabVIEW, a machine vision program for the automatic sorting of membrane\|removed mandarin segments was designed. An experimental platform was built with color GigE camera, white ring light and red flat light, to test inspection accuracy and duration. It was shown that the accuracy of inspection program reached (99.1±0.5)%, and the average time consumption was (17±5) ms. The inspect speed was able to achieve (64±19) pieces per second.
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    Study on vegetable seed vigor index detection algorithm and system realization based on machine vision
    LI Zhen1,2, LIAO Tong\|qing1, FENG Qing\|chun2,3,4,5, ZHANG Dong\|yan1,2,3,4,5, WANG Xiu2,3,4,5,*
    2015, 27(12):  2218. 
    Abstract ( 416 )   PDF (1577KB) ( 1307 )  
    Conventional vigor tests couldnt meet current demand for the development of automatic breeding because of their subjectivity, high cost and difficult operations. In order to improve the automatic level and quality of seed vigor detection, chili seed images were used to study the detection method. The innovative gray transformation was used to process seed\|color images. This method could protect seed information enough. Combining self\|developed system characteristics, threshold processing was used to remove noise, which could reduce the time of system processing seed images. For marking every seed, the paper used cvFloodFill algorithm to fill seeds into different gray values. Then the seed germination index and average seed radicle length could be acquired by self\|developed software. Finally we combined system hardware and software perfectly. Through processing different seed images, the result showed the system algorithm had certain advantages in the processing of such images. For assessing precision of system through calculation of chili seed vigor index, the results showed that the accuracy of seed vigor index detection was greatly high, surpass 92%. So this system could provide research foundation for online production of vegetable seeds.
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    Plant leaf recognition based on artificial neural network ensemble
    EN De, HU Sheng\|qiang
    2015, 27(12):  2225. 
    Abstract ( 447 )   PDF (1381KB) ( 1365 )  
    To improve the accuracy of the automatic plant identification system, this paper proposed a novel methodology of characterizing and recognizing plant leaves using shape and texture features with neural network ensemble. Shape features of the leaves were captured using invariant moments together with geometric parameter of leaves. Texture of the leaf was modeled using gray level co\|occurrence matrix (GLCM). And an artificial neural network ensemble in which the base classifier was composed by the union of a binary classifier and a multiclass classifier was used for the resolution of multi\|class problems. Since some of these features were in general sensitive to the orientation of the leaf images, a pre\|processing stage prior to feature extraction was applied to make corrections for varying rotation. After the pre\|processing stage, the neural network ensemble was used to train and classify the plant leaf samples. Experimentations to demonstrate the efficacy of the proposed approach were performed on a dataset of 600 images divided into 20 classes with 30 images per class, collected from Flavia, and the accuracy was 91%. In contrast with the other plant leaf recognition methods, the results showed that this method could significantly improve the accuracy of the system.
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    Current situation of six industrialization of agriculture and its main characteristics from perspectives of against social division: Japans case and its enlightenments to China
    ZHU Fu\|yuna, KE Fu\|yanb
    2015, 27(12):  2234. 
    Abstract ( 366 )   PDF (727KB) ( 1356 )  
    To increase the small and medium sized farmers income and promote rural economic development, Japan formally promoted the six industrialization of agriculture from the mid\|90s of 20th century. Combining literatures and field surveys, it is found that three against social divisions characteristics including production integration organization, local consumption of circulation, and homegrown marketing were the crucial keys to its success. Up to now, these successful experiences still shed enlightenments to China: strengthen the direct docking of farmers and marketing, popularize the local consumption of farm products, and promote the training of new\|type talented farmers.
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    Analysis on spatial effect, output elasticity and technical discrepancy of agricultural production: An example from Beijing and Tianjin counties
    CUI Kai, LI Ning\|hui, GUO Jing\|li*
    2015, 27(12):  2240. 
    Abstract ( 368 )   PDF (1427KB) ( 1269 )  
    As neoclassical input\|output model combined with space effect, spatial econometrics method was used to study spatial correlation and measure output elasticity of agricultural production in Beijing and Tianjins 22 counties. Then regional difference in agricultural technology was decomposed. Result showed that agricultural production performed local accumulation in spatial distribution and positive spatial lag effect. Labor and capital was determinant factor in agricultural production, while lands contribution was not significant. For total regional differences, agricultural technology discrepancy was mainly reflected within region, but not among different regions. Considering similarity and interaction in agricultural production activities around neighborhood, spatial interaction needs to be considered in policy setting.
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  • 2 2020-12-04
  • 1 2020-12-04
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