Abstract： Overexpression is an important approach for functional characterization of plant genes and genetic improvement of crop plants. To improve the efficiency of characterization of transgenic plants， a transformation vector with the maize （Zea mays） ubiquitin\|1 promoter （P\|Ubi） and the pea （Pisum sativum） T3A\|polyA sequence in the overexpression cassette was constructed using the mCherry red fluorescent protein as a reporter. The coding sequences of four receptor\|like kinases of rice （Oryza sativa） were inserted into the overexpression cassette of the vector and then transformed into the rice cultivar Taijing\|394， respectively. mCherry fluorescence assay of the T1 transgenic seeds showed that on average， 625% of the T1 lines segregated with a 3∶1 ratio. Based on the results of real\|time quantitative reverse\|transcription polymerase chain reaction assays， the relative gene\|expression levels of the overexpressed genes in the transgene\|positive plants were significantly higher than those of the genes in the negative segregant and untransformed recipient cultivar. The vector system can be used for large\|scale screening of transgenic progeny and obtaining of transgenic lines with target genes overexpressed， so as to promote the functional characterization of plant genes and the application of transgenic technology.
