烟台黑猪SLA-DQB基因外显子2多态性及其与仔猪腹泻的关联分析

doi:10.3969/j.issn.1004-1524.2016.10.07

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (10): 1671-1677.DOI: 10.3969/j.issn.1004-1524.2016.10.07

烟台黑猪SLA-DQB基因外显子2多态性及其与仔猪腹泻的关联分析

母童, 王国梅, 杨启瑞, 金丽, 张海龙, 张丽, 田晓静, 刘丽霞^*

西北民族大学生命科学与工程学院,甘肃兰州 730030

收稿日期:2016-03-05 出版日期:2016-10-15 发布日期:2016-11-20
通讯作者: 刘丽霞,E-mail:skllx@xbmu.edu.cn
作者简介:母童(1995—),男,宁夏银川人,本科生,主要从事分子生物学方面的研究。E-mail:1227415848@qq.com
基金资助:
中央高校基本科研业务费专项资金本科生项目(107422015033); 西北民族大学中央高校基本科研业务费专项资金资助项目(31920130048); 西北民族大学引进人才项目(xbmuyjrc201408)

Polymorphism of SLA-DQB exon 2 and its association with diarrhea in Yantai black pigs

MU Tong, WANG Guo-mei, YANG Qi-rui, JIN Li, ZHANG Hai-long, ZHANG Li, TIAN Xiao-jing, LIU Li-xia^*

College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China

Received:2016-03-05 Online:2016-10-15 Published:2016-11-20

摘要/Abstract

摘要： 采用PCR-RFLP方法对烟台黑猪DQB基因外显子2的多态性进行研究,并对该位点与仔猪腹泻的关联进行了分析。结果表明,在烟台黑猪DQB基因外显子2上共检测出4种等位基因(A、B、C、D),7种RFLP带型(AA、BB、CC、AB、AC、BC、AD),该位点具有较丰富的多态性。卡方适合性检验显示,烟台黑猪的DQB基因外显子2没有达到Hardy-Weinberg平衡(P<0.05)。最小二乘法分析结果表明,烟台黑猪的性别和DQB外显子2的基因型均对仔猪腹泻无显著影响(P>0.05),而性别与基因型的互作效应对仔猪腹泻具有极显著的影响(P<0.01),在公猪群体中,AD基因型仔猪腹泻评分高于AA基因型个体(P<0.05),在母猪群体中BB、AC、BC、CC基因型仔猪腹泻评分高于其他基因型仔猪(P<0.05)。

关键词: 烟台黑猪, SLA-DQB, 仔猪腹泻, PCR-RFLP, 多态性

Abstract: Polymorphism of SLA-DQB exon 2 in Yantai black pigs was studied using PCR-RFLP, and its correlation with piglets diarrhea was analyzed. The results showed that there were four alleles (A, B, C, D) and seven RFLP genotypes (AA, BB, CC, AB, AC, BC, AD), polymorphism of the DQB exon 2 was abundant. The chi-square test displayed DQB exon 2 of Yantai black pigs deviated Hardy-Weinberg equilibrium(P<0.05). The results of least squares showed that the sex of Yantai black pig and genotype of DQB exon 2 did not have significant influence on diarrhea piglets (P>0.05). However, polymorphism of DQB exon 2 in different genders affected diarrhea piglets (P<0.01). In males, diarrhea score of genotype AD was higher than that of AA genotype (P<0.05), in female, scores of BB, AC, BC, CC were higher than others (P<0.05).

Key words: Yantai black pig, SLA-DQB, piglet diarrhea, PCR-RFLP, polymorphism

中图分类号:

S858.28

母童, 王国梅, 杨启瑞, 金丽, 张海龙, 张丽, 田晓静, 刘丽霞. 烟台黑猪SLA-DQB基因外显子2多态性及其与仔猪腹泻的关联分析[J]. 浙江农业学报, 2016, 28(10): 1671-1677.

MU Tong, WANG Guo-mei, YANG Qi-rui, JIN Li, ZHANG Hai-long, ZHANG Li, TIAN Xiao-jing, LIU Li-xia. Polymorphism of SLA-DQB exon 2 and its association with diarrhea in Yantai black pigs[J]. , 2016, 28(10): 1671-1677.

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烟台黑猪SLA-DQB基因外显子2多态性及其与仔猪腹泻的关联分析

Polymorphism of SLA-DQB exon 2 and its association with diarrhea in Yantai black pigs

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