大豆PP2C家族基因鉴定与响应盐胁迫的转录组分析

doi:10.3969/j.issn.1004-1524.2022.02.01

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (2): 207-220.DOI: 10.3969/j.issn.1004-1524.2022.02.01

大豆PP2C家族基因鉴定与响应盐胁迫的转录组分析

杨昕霞^a(), 唐满生^b, 张斌^b^,^*()

a.湖南科技学院,后勤处,湖南永州 425199
b.湖南科技学院,化学与生物工程学院,湖南永州 425199

收稿日期:2021-05-21 出版日期:2022-02-25 发布日期:2022-03-02
通讯作者: 张斌
作者简介:张斌,E-mail: zhangbin27104@163.com
杨昕霞(1982—),女,湖南永州人,学士,副教授,研究方向为植物资源利用。E-mail: 76406391@qq.com
基金资助:
湖南省自然科学基金(2020JJ4030);湖南科技学院重点项目(17XKY012)

Identification of soybean PP2C family genes and transcriptome analysis in response to salt stress

YANG Xinxia^a(), TANG Mansheng^b, ZHANG Bin^b^,^*()

a. Department of Logistics, Hunan University of Science and Engineering, Yongzhou 425199, Hunan, China
b. College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, Hunan, China

Received:2021-05-21 Online:2022-02-25 Published:2022-03-02
Contact: ZHANG Bin

摘要/Abstract

摘要：

植物蛋白磷酸酶2C(PP2C)能够通过影响植物体内多种生物学过程在环境胁迫响应中发挥重要作用。为研究栽培大豆PP2C家族成员在盐胁迫中的功能,通过生物信息学手段结合转录组学分析对栽培大豆PP2C家族进行了系统探究。在栽培大豆中共鉴定出126个PP2C家族成员,并将其在进化树上分为11个亚家族,同一亚家族的成员具有类似的基因和蛋白结构。盐胁迫转录组测序结果显示,大豆126个GmPP2C基因中有9个是差异表达基因,其中,GmPP2C41、GmPP2C48、GmPP2C72、GmPP2C84、GmPP2C89、GmPP2C107和GmPP2C113在盐处理后表达水平上调,GmPP2C27和GmPP2C114在盐处理后表达下调。qRT-PCR结果显示,GmPP2C89和GmPP2C113在盐处理6 h表达水平上调最高。GO富集分析发现,9个差异基因主要具有离子结合和磷酸酶活性;Y2H结果显示,GmPP2C113和GmPP2C47能够互作。以上结果将为进一步研究大豆PP2C家族成员在盐胁迫响应中的功能与分子机制提供理论依据,为大豆耐盐性遗传改良提供重要候选基因。

关键词: 大豆, 蛋白磷酸酶, 盐胁迫, 转录组, 差异表达基因

Abstract:

Protein phosphatases (PP2Cs) plays important roles in response to environmental stress by affecting a variety of biological processes in plants. In order to explore the function of members of PP2C family of cultivated soybeans in salt stress, PP2C family of cultivated soybeans was systematically explored through bioinformatics and transcriptom analysis. A total of 126 PP2C members were identified in this study and were divided into 11 subfamilies, and PP2Cs in the same subfamily have similar gene and protein structures. Transcriptome sequencing results showed that 9 GmPP2Cs were differentially expressed genes (DEGs) under salt stress, of which GmPP2C41, GmPP2C48, GmPP2C72, GmPP2C84, GmPP2C89, GmPP2C107 and GmPP2C113 were up-regulated after salt treatment, while GmPP2C27 and GmPP2C114 were down-regulated. qRT-PCR results showed that GmPP2C89 and GmPP2C113 had the highest expression level at 6 h after salt treatment. GO enrichment analysis found that the nine DEGs mainly had ion binding and phosphatase activity. The Y2H results showed that GmPP2C113 and GmPP2C47 could interact. Therefore, the above results would provide a theoretical basis for further research on the functions and molecular mechanisms of soybean PP2C family members in response to salt stress and provide important candidate genes for the genetic improvement of soybean salt tolerance.

Key words: soybean (Glycine max), type 2C protein phosphatase, salt stress, transcriptome analysis, differentially expressed genes

中图分类号:

S565.1

杨昕霞, 唐满生, 张斌. 大豆PP2C家族基因鉴定与响应盐胁迫的转录组分析[J]. 浙江农业学报, 2022, 34(2): 207-220.

YANG Xinxia, TANG Mansheng, ZHANG Bin. Identification of soybean PP2C family genes and transcriptome analysis in response to salt stress[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 207-220.

图/表 8

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基因名 Gene name	正向引物 Forward primer(5'-3')	反向引物 Reverse primer(5'-3')	片段长度 Fragment length/bp
GmPP2C27	AGGCTGCAGCAACCCATATT	GGCACAGAAGTAGGTGGCTT	115
GmPP2C41	CCGCTTCATTTCTTCGGCG	CTTCTCTGCCATTCAGCGGC	134
GmPP2C48	GTCTATGATGGGCATGGGGG	CCGTCCCTCCCATTCTTTCC	122
GmPP2C72	GCCAAAGTGGAAATCCACGAC	CCATGTCTCTTCTCCTGCCG	124
GmPP2C84	CCGAGACTTCTTCTCCCATCG	GAGAAGCGCGCCTATCCAG	150
GmPP2C89	TCTTCCCTGCGAGTGGAGTC	GGATCTTCGTCTGGCAAGTGT	112
GmPP2C107	ACTGGACACAAGGTGACGAA	TCACGCCGAACTTGGGAGAT	118
GmPP2C113	ACCAGAAGTGACAGTGACCC	GATTCCCAACTCCATCGCAC	158
GmPP2C114	GATCACCTCTTCCAGCACCT	ACACCAACCAGACAACAGGA	173

基因名 Gene name	正向引物 Forward primer(5'-3')	反向引物 Reverse primer(5'-3')	片段长度 Fragment length/bp
GmPP2C27	AGGCTGCAGCAACCCATATT	GGCACAGAAGTAGGTGGCTT	115
GmPP2C41	CCGCTTCATTTCTTCGGCG	CTTCTCTGCCATTCAGCGGC	134
GmPP2C48	GTCTATGATGGGCATGGGGG	CCGTCCCTCCCATTCTTTCC	122
GmPP2C72	GCCAAAGTGGAAATCCACGAC	CCATGTCTCTTCTCCTGCCG	124
GmPP2C84	CCGAGACTTCTTCTCCCATCG	GAGAAGCGCGCCTATCCAG	150
GmPP2C89	TCTTCCCTGCGAGTGGAGTC	GGATCTTCGTCTGGCAAGTGT	112
GmPP2C107	ACTGGACACAAGGTGACGAA	TCACGCCGAACTTGGGAGAT	118
GmPP2C113	ACCAGAAGTGACAGTGACCC	GATTCCCAACTCCATCGCAC	158
GmPP2C114	GATCACCTCTTCCAGCACCT	ACACCAACCAGACAACAGGA	173

基因名 Gene name	基因登录号 Gene ID	外显子数量 Exon count	氨基酸残基 Peptide residues	分子量 Molecular weight/ku	等电点 pI	不稳定系数 Instability index	亲水性 Grand average of hydropathicity
GmPP2C1	GLYMA.01G127600	4	290	32 192.77	6.65	37.11	-0.267
GmPP2C2	GLYMA.01G158000	4	374	40 365.95	5.42	55.69	-0.200
GmPP2C3	GLYMA.01G191300	4	377	41 796.69	6.43	40.61	-0.200
GmPP2C4	GLYMA.01G199600	4	753	83 208.77	5.21	50.37	-0.575
GmPP2C5	GLYMA.01G225100	3	324	35 093.05	6.75	47.55	-0.228
GmPP2C6	GLYMA.02G009100	4	396	43 461.91	4.78	51.36	-0.213
GmPP2C7	GLYMA.02G044700	5	394	43 795.09	8.79	47.28	-0.250
GmPP2C8	GLYMA.02G058600	4	364	39 141.87	5.69	34.82	-0.152
GmPP2C9	GLYMA.02G144500	22	1 073	121 060.70	5.77	38.93	-0.383
GmPP2C10	GLYMA.02G228200	4	389	41 812.92	6.28	55.94	-0.294
GmPP2C11	GLYMA.02G250200	4	407	44 933.13	6.67	53.82	-0.361
GmPP2C12	GLYMA.03G043100	5	211	23 690.01	7.67	37.86	-0.332
GmPP2C13	GLYMA.03G044100	3	222	24 615.08	6.43	40.96	-0.285
GmPP2C14	GLYMA.03G176100	5	357	39 901.23	6.84	41.18	-0.287
GmPP2C15	GLYMA.03G225800	4	887	99 448.89	6.01	49.22	-0.544
GmPP2C16	GLYMA.03G233300	8	426	45 561.23	7.98	38.38	-0.074
GmPP2C17	GLYMA.03G233500	8	371	41 097.80	6.64	39.60	-0.246
GmPP2C18	GLYMA.04G038000	11	429	46 556.83	5.28	41.90	-0.288
GmPP2C19	GLYMA.04G053800	6	544	59 216.90	4.86	43.36	-0.216
GmPP2C20	GLYMA.04G059000	9	312	33 540.32	5.02	37.01	-0.258
GmPP2C21	GLYMA.04G069900	4	370	40 592.64	5.29	58.10	-0.389
GmPP2C22	GLYMA.04G101400	6	301	33 228.71	7.70	41.29	-0.313
GmPP2C23	GLYMA.05G113400	4	696	76 424.07	5.57	45.86	-0.371
GmPP2C24	GLYMA.05G116800	6	282	30 673.49	6.66	38.30	-0.357
GmPP2C25	GLYMA.05G227100	4	384	42 155.30	5.36	67.38	-0.348
GmPP2C26	GLYMA.06G016100	4	385	41 908.80	5.28	52.86	-0.179
GmPP2C27	GLYMA.06G039000	9	429	46 694.27	5.29	41.02	-0.230
GmPP2C28	GLYMA.06G050900	5	343	37 342.22	5.35	33.80	-0.234
GmPP2C29	GLYMA.06G054000	6	544	58 934.53	4.83	45.69	-0.182
GmPP2C30	GLYMA.06G059700	9	314	33 766.48	5.18	40.20	-0.327
GmPP2C31	GLYMA.06G071900	5	370	40 595.79	5.30	56.73	-0.365
GmPP2C32	GLYMA.06G103100	6	293	32 415.53	7.09	41.39	-0.432
GmPP2C33	GLYMA.06G130900	11	392	43 150.91	5.84	50.68	-0.155
GmPP2C34	GLYMA.06G238200	5	282	30 849.97	5.87	41.78	-0.313
GmPP2C35	GLYMA.06G296200	5	471	51 859.97	5.50	43.70	-0.504
GmPP2C36	GLYMA.07G131800	4	602	66 123.01	5.13	38.86	-0.359
GmPP2C37	GLYMA.07G233000	2	386	42 549.56	5.04	61.31	-0.056
GmPP2C38	GLYMA.07G239000	5	374	41 517.38	7.81	35.89	-0.192
GmPP2C39	GLYMA.07G244700	7	367	41 031.05	8.29	30.04	-0.273
GmPP2C40	GLYMA.07G248000	5	520	56 834.23	4.99	41.85	-0.192
GmPP2C41	GLYMA.08G033800	4	385	42 285.55	5.26	63.59	-0.343
GmPP2C42	GLYMA.08G071900	7	282	30 694.42	6.17	42.66	-0.362
GmPP2C43	GLYMA.08G081400	8	351	39 455.77	4.86	43.14	-0.695
GmPP2C44	GLYMA.08G178800	5	280	30 455.45	6.25	34.28	-0.323
GmPP2C45	GLYMA.09G031700	2	405	44 584.63	5.01	61.41	-0.104
GmPP2C46	GLYMA.09G034800	4	374	41 362.15	7.25	44.03	-0.129
GmPP2C47	GLYMA.09G045100	6	409	45 167.96	4.84	38.69	-0.224
GmPP2C48	GLYMA.09G066500	5	522	57 118.04	5.42	42.20	-0.545
GmPP2C49	GLYMA.09G096600	4	381	42 730.21	5.04	44.72	-0.384
GmPP2C50	GLYMA.09G111300	5	385	43 080.32	8.71	46.63	-0.205
GmPP2C51	GLYMA.09G249700	5	489	54 209.69	5.53	46.34	-0.556
GmPP2C52	GLYMA.09G280700	5	434	48 642.41	5.11	42.79	-0.504
GmPP2C53	GLYMA.10G004200	5	282	32 656.61	9.57	47.97	-0.498
GmPP2C54	GLYMA.10G009800	5	396	43 572.14	4.76	49.32	-0.154
GmPP2C55	GLYMA.10G047500	5	371	40 953.34	6.43	42.05	-0.316
GmPP2C56	GLYMA.10G149600	8	428	45 996.65	7.49	37.66	-0.149
GmPP2C57	GLYMA.10G150100	7	368	41 126.43	5.78	39.61	-0.360
GmPP2C58	GLYMA.10G182400	6	277	31 868.60	9.17	54.09	-0.455
GmPP2C59	GLYMA.10G259400	4	378	42 452.47	8.13	42.60	-0.233
GmPP2C60	GLYMA.10G271500	11	431	46 614.22	7.99	38.89	-0.240
GmPP2C61	GLYMA.10G282900	5	397	43 516.33	7.28	51.12	-0.222
GmPP2C62	GLYMA.10G291800	9	320	34 490.27	5.02	41.37	-0.275
GmPP2C63	GLYMA.10G294400	5	389	43 106.25	8.10	46.29	-0.246
GmPP2C64	GLYMA.10G297900	2	208	22 659.85	5.57	32.40	0.014
GmPP2C65	GLYMA.11G018000	4	336	36 484.61	7.89	45.57	-0.240
GmPP2C66	GLYMA.11G042300	4	739	82 047.64	5.28	53.20	-0.576
GmPP2C67	GLYMA.11G050900	4	375	41 826.79	5.83	40.09	-0.194
GmPP2C68	GLYMA.11G087000	4	374	40 313.84	5.51	56.60	-0.100
GmPP2C69	GLYMA.11G139500	4	720	79 617.51	5.53	36.87	-0.449
GmPP2C70	GLYMA.11G170200	4	335	36 549.43	6.71	51.93	-0.293
GmPP2C71	GLYMA.11G171600	4	336	36 663.53	6.71	51.81	-0.302
GmPP2C72	GLYMA.11G222600	4	401	43 834.33	5.51	56.39	-0.438
GmPP2C73	GLYMA.12G063100	4	720	79 940.84	5.37	40.75	-0.482
GmPP2C74	GLYMA.12G109500	5	471	51 711.72	5.30	42.12	-0.494
GmPP2C75	GLYMA.12G116800	5	281	31 172.50	6.07	39.18	-0.359
GmPP2C76	GLYMA.12G202400	7	474	51 766.93	5.04	45.62	-0.392
GmPP2C77	GLYMA.12G226100	5	265	29 350.15	6.51	53.65	-0.459
GmPP2C78	GLYMA.13G042100	11	356	39 260.01	5.17	42.38	-0.346
GmPP2C79	GLYMA.13G106800	6	536	58 563.88	5.07	47.95	-0.386
GmPP2C80	GLYMA.13G135300	5	371	40 947.29	6.47	40.61	-0.342
GmPP2C81	GLYMA.13G165300	4	383	42 454.17	5.18	49.88	-0.364
GmPP2C82	GLYMA.13G274800	5	283	31 107.25	7.70	47.17	-0.433
GmPP2C83	GLYMA.13G299300	5	475	52 153.36	5.31	46.32	-0.446
GmPP2C84	GLYMA.14G066400	4	400	44 150.19	6.05	60.37	-0.345
GmPP2C85	GLYMA.14G082200	11	428	46 492.90	5.29	42.01	-0.276
GmPP2C86	GLYMA.14G104900	9	338	36 587.85	5.15	44.51	-0.193
GmPP2C87	GLYMA.14G108400	5	557	60 815.60	4.62	41.13	-0.205
GmPP2C88	GLYMA.14G161400	10	356	39 293.99	5.47	41.18	-0.426
GmPP2C89	GLYMA.14G162100	3	386	42 074.51	6.73	46.77	-0.333
GmPP2C90	GLYMA.14G195200	4	390	41 811.95	6.11	55.12	-0.261
GmPP2C91	GLYMA.15G053700	5	278	30 487.53	6.31	35.14	-0.282
GmPP2C92	GLYMA.15G139500	4	372	41 084.92	7.69	46.14	-0.123
GmPP2C93	GLYMA.15G172500	8	527	57 849.70	5.22	44.75	-0.541
GmPP2C94	GLYMA.15G201300	4	379	42 328.00	5.46	45.59	-0.353
GmPP2C95	GLYMA.16G122200	6	394	43 876.13	8.79	47.20	-0.263
GmPP2C96	GLYMA.17G026100	5	524	57 138.86	5.52	37.84	-0.167
GmPP2C97	GLYMA.17G029200	7	376	41 908.18	7.99	29.58	-0.233
GmPP2C98	GLYMA.17G034500	4	375	41 437.23	7.80	33.16	-0.208
GmPP2C99	GLYMA.17G038000	2	380	41 708.68	5.09	63.88	-0.022
GmPP2C100	GLYMA.17G052500	7	538	58 548.81	4.97	48.37	-0.374
GmPP2C101	GLYMA.17G105800	4	383	42 290.77	5.11	47.42	-0.344
GmPP2C102	GLYMA.17G153800	5	701	77 629.50	5.50	49.90	-0.447
GmPP2C103	GLYMA.17G218700	8	558	60 792.54	4.62	38.16	-0.208
GmPP2C104	GLYMA.17G221100	9	338	36 655.97	5.09	41.29	-0.188
GmPP2C105	GLYMA.17G230600	5	344	37 627.48	5.07	31.99	-0.240
GmPP2C106	GLYMA.17G243100	12	428	46 575.99	5.23	45.89	-0.294
GmPP2C107	GLYMA.18G035000	4	400	44 067.66	5.17	58.50	-0.443
GmPP2C108	GLYMA.18G061000	4	347	37 640.64	5.81	51.88	-0.222
GmPP2C109	GLYMA.18G180400	4	597	65 974.13	5.37	37.54	-0.349
GmPP2C110	GLYMA.18G208100	5	424	47 458.29	5.24	45.39	-0.473
GmPP2C111	GLYMA.18G242700	6	487	54 026.40	6.10	42.66	-0.578
GmPP2C112	GLYMA.18G283300	6	455	50 115.64	5.30	37.19	-0.325
GmPP2C113	GLYMA.19G069200	3	377	40 769.73	5.68	51.67	-0.354
GmPP2C114	GLYMA.19G147800	4	391	44 150.56	7.69	53.67	-0.278
GmPP2C115	GLYMA.19G176600	5	370	41 129.64	8.22	40.69	-0.272
GmPP2C116	GLYMA.19G222700	4	887	99 137.72	6.01	49.65	-0.516
GmPP2C117	GLYMA.19G230200	8	429	46 091.00	7.97	36.52	-0.092
GmPP2C118	GLYMA.19G230600	9	369	40 812.38	6.09	35.91	-0.243
GmPP2C119	GLYMA.20G106800	6	397	43 657.42	7.28	50.98	-0.270
GmPP2C120	GLYMA.20G119100	12	431	46 513.07	7.56	38.39	-0.235
GmPP2C121	GLYMA.20G131500	4	373	41 357.11	6.93	40.87	-0.180
GmPP2C122	GLYMA.20G207600	6	277	31 730.49	9.44	51.96	-0.467
GmPP2C123	GLYMA.20G238100	8	367	41 147.49	6.14	41.32	-0.389
GmPP2C124	GLYMA.20G238700	8	428	46 223.91	6.91	37.92	-0.181
GmPP2C125	GLYMA.20G244300	5	388	42 720.79	6.87	44.45	-0.222
GmPP2C126	GLYMA.20G248900	3	365	39 896.10	7.16	37.52	-0.239

基因名 Gene name	基因登录号 Gene ID	外显子数量 Exon count	氨基酸残基 Peptide residues	分子量 Molecular weight/ku	等电点 pI	不稳定系数 Instability index	亲水性 Grand average of hydropathicity
GmPP2C1	GLYMA.01G127600	4	290	32 192.77	6.65	37.11	-0.267
GmPP2C2	GLYMA.01G158000	4	374	40 365.95	5.42	55.69	-0.200
GmPP2C3	GLYMA.01G191300	4	377	41 796.69	6.43	40.61	-0.200
GmPP2C4	GLYMA.01G199600	4	753	83 208.77	5.21	50.37	-0.575
GmPP2C5	GLYMA.01G225100	3	324	35 093.05	6.75	47.55	-0.228
GmPP2C6	GLYMA.02G009100	4	396	43 461.91	4.78	51.36	-0.213
GmPP2C7	GLYMA.02G044700	5	394	43 795.09	8.79	47.28	-0.250
GmPP2C8	GLYMA.02G058600	4	364	39 141.87	5.69	34.82	-0.152
GmPP2C9	GLYMA.02G144500	22	1 073	121 060.70	5.77	38.93	-0.383
GmPP2C10	GLYMA.02G228200	4	389	41 812.92	6.28	55.94	-0.294
GmPP2C11	GLYMA.02G250200	4	407	44 933.13	6.67	53.82	-0.361
GmPP2C12	GLYMA.03G043100	5	211	23 690.01	7.67	37.86	-0.332
GmPP2C13	GLYMA.03G044100	3	222	24 615.08	6.43	40.96	-0.285
GmPP2C14	GLYMA.03G176100	5	357	39 901.23	6.84	41.18	-0.287
GmPP2C15	GLYMA.03G225800	4	887	99 448.89	6.01	49.22	-0.544
GmPP2C16	GLYMA.03G233300	8	426	45 561.23	7.98	38.38	-0.074
GmPP2C17	GLYMA.03G233500	8	371	41 097.80	6.64	39.60	-0.246
GmPP2C18	GLYMA.04G038000	11	429	46 556.83	5.28	41.90	-0.288
GmPP2C19	GLYMA.04G053800	6	544	59 216.90	4.86	43.36	-0.216
GmPP2C20	GLYMA.04G059000	9	312	33 540.32	5.02	37.01	-0.258
GmPP2C21	GLYMA.04G069900	4	370	40 592.64	5.29	58.10	-0.389
GmPP2C22	GLYMA.04G101400	6	301	33 228.71	7.70	41.29	-0.313
GmPP2C23	GLYMA.05G113400	4	696	76 424.07	5.57	45.86	-0.371
GmPP2C24	GLYMA.05G116800	6	282	30 673.49	6.66	38.30	-0.357
GmPP2C25	GLYMA.05G227100	4	384	42 155.30	5.36	67.38	-0.348
GmPP2C26	GLYMA.06G016100	4	385	41 908.80	5.28	52.86	-0.179
GmPP2C27	GLYMA.06G039000	9	429	46 694.27	5.29	41.02	-0.230
GmPP2C28	GLYMA.06G050900	5	343	37 342.22	5.35	33.80	-0.234
GmPP2C29	GLYMA.06G054000	6	544	58 934.53	4.83	45.69	-0.182
GmPP2C30	GLYMA.06G059700	9	314	33 766.48	5.18	40.20	-0.327
GmPP2C31	GLYMA.06G071900	5	370	40 595.79	5.30	56.73	-0.365
GmPP2C32	GLYMA.06G103100	6	293	32 415.53	7.09	41.39	-0.432
GmPP2C33	GLYMA.06G130900	11	392	43 150.91	5.84	50.68	-0.155
GmPP2C34	GLYMA.06G238200	5	282	30 849.97	5.87	41.78	-0.313
GmPP2C35	GLYMA.06G296200	5	471	51 859.97	5.50	43.70	-0.504
GmPP2C36	GLYMA.07G131800	4	602	66 123.01	5.13	38.86	-0.359
GmPP2C37	GLYMA.07G233000	2	386	42 549.56	5.04	61.31	-0.056
GmPP2C38	GLYMA.07G239000	5	374	41 517.38	7.81	35.89	-0.192
GmPP2C39	GLYMA.07G244700	7	367	41 031.05	8.29	30.04	-0.273
GmPP2C40	GLYMA.07G248000	5	520	56 834.23	4.99	41.85	-0.192
GmPP2C41	GLYMA.08G033800	4	385	42 285.55	5.26	63.59	-0.343
GmPP2C42	GLYMA.08G071900	7	282	30 694.42	6.17	42.66	-0.362
GmPP2C43	GLYMA.08G081400	8	351	39 455.77	4.86	43.14	-0.695
GmPP2C44	GLYMA.08G178800	5	280	30 455.45	6.25	34.28	-0.323
GmPP2C45	GLYMA.09G031700	2	405	44 584.63	5.01	61.41	-0.104
GmPP2C46	GLYMA.09G034800	4	374	41 362.15	7.25	44.03	-0.129
GmPP2C47	GLYMA.09G045100	6	409	45 167.96	4.84	38.69	-0.224
GmPP2C48	GLYMA.09G066500	5	522	57 118.04	5.42	42.20	-0.545
GmPP2C49	GLYMA.09G096600	4	381	42 730.21	5.04	44.72	-0.384
GmPP2C50	GLYMA.09G111300	5	385	43 080.32	8.71	46.63	-0.205
GmPP2C51	GLYMA.09G249700	5	489	54 209.69	5.53	46.34	-0.556
GmPP2C52	GLYMA.09G280700	5	434	48 642.41	5.11	42.79	-0.504
GmPP2C53	GLYMA.10G004200	5	282	32 656.61	9.57	47.97	-0.498
GmPP2C54	GLYMA.10G009800	5	396	43 572.14	4.76	49.32	-0.154
GmPP2C55	GLYMA.10G047500	5	371	40 953.34	6.43	42.05	-0.316
GmPP2C56	GLYMA.10G149600	8	428	45 996.65	7.49	37.66	-0.149
GmPP2C57	GLYMA.10G150100	7	368	41 126.43	5.78	39.61	-0.360
GmPP2C58	GLYMA.10G182400	6	277	31 868.60	9.17	54.09	-0.455
GmPP2C59	GLYMA.10G259400	4	378	42 452.47	8.13	42.60	-0.233
GmPP2C60	GLYMA.10G271500	11	431	46 614.22	7.99	38.89	-0.240
GmPP2C61	GLYMA.10G282900	5	397	43 516.33	7.28	51.12	-0.222
GmPP2C62	GLYMA.10G291800	9	320	34 490.27	5.02	41.37	-0.275
GmPP2C63	GLYMA.10G294400	5	389	43 106.25	8.10	46.29	-0.246
GmPP2C64	GLYMA.10G297900	2	208	22 659.85	5.57	32.40	0.014
GmPP2C65	GLYMA.11G018000	4	336	36 484.61	7.89	45.57	-0.240
GmPP2C66	GLYMA.11G042300	4	739	82 047.64	5.28	53.20	-0.576
GmPP2C67	GLYMA.11G050900	4	375	41 826.79	5.83	40.09	-0.194
GmPP2C68	GLYMA.11G087000	4	374	40 313.84	5.51	56.60	-0.100
GmPP2C69	GLYMA.11G139500	4	720	79 617.51	5.53	36.87	-0.449
GmPP2C70	GLYMA.11G170200	4	335	36 549.43	6.71	51.93	-0.293
GmPP2C71	GLYMA.11G171600	4	336	36 663.53	6.71	51.81	-0.302
GmPP2C72	GLYMA.11G222600	4	401	43 834.33	5.51	56.39	-0.438
GmPP2C73	GLYMA.12G063100	4	720	79 940.84	5.37	40.75	-0.482
GmPP2C74	GLYMA.12G109500	5	471	51 711.72	5.30	42.12	-0.494
GmPP2C75	GLYMA.12G116800	5	281	31 172.50	6.07	39.18	-0.359
GmPP2C76	GLYMA.12G202400	7	474	51 766.93	5.04	45.62	-0.392
GmPP2C77	GLYMA.12G226100	5	265	29 350.15	6.51	53.65	-0.459
GmPP2C78	GLYMA.13G042100	11	356	39 260.01	5.17	42.38	-0.346
GmPP2C79	GLYMA.13G106800	6	536	58 563.88	5.07	47.95	-0.386
GmPP2C80	GLYMA.13G135300	5	371	40 947.29	6.47	40.61	-0.342
GmPP2C81	GLYMA.13G165300	4	383	42 454.17	5.18	49.88	-0.364
GmPP2C82	GLYMA.13G274800	5	283	31 107.25	7.70	47.17	-0.433
GmPP2C83	GLYMA.13G299300	5	475	52 153.36	5.31	46.32	-0.446
GmPP2C84	GLYMA.14G066400	4	400	44 150.19	6.05	60.37	-0.345
GmPP2C85	GLYMA.14G082200	11	428	46 492.90	5.29	42.01	-0.276
GmPP2C86	GLYMA.14G104900	9	338	36 587.85	5.15	44.51	-0.193
GmPP2C87	GLYMA.14G108400	5	557	60 815.60	4.62	41.13	-0.205
GmPP2C88	GLYMA.14G161400	10	356	39 293.99	5.47	41.18	-0.426
GmPP2C89	GLYMA.14G162100	3	386	42 074.51	6.73	46.77	-0.333
GmPP2C90	GLYMA.14G195200	4	390	41 811.95	6.11	55.12	-0.261
GmPP2C91	GLYMA.15G053700	5	278	30 487.53	6.31	35.14	-0.282
GmPP2C92	GLYMA.15G139500	4	372	41 084.92	7.69	46.14	-0.123
GmPP2C93	GLYMA.15G172500	8	527	57 849.70	5.22	44.75	-0.541
GmPP2C94	GLYMA.15G201300	4	379	42 328.00	5.46	45.59	-0.353
GmPP2C95	GLYMA.16G122200	6	394	43 876.13	8.79	47.20	-0.263
GmPP2C96	GLYMA.17G026100	5	524	57 138.86	5.52	37.84	-0.167
GmPP2C97	GLYMA.17G029200	7	376	41 908.18	7.99	29.58	-0.233
GmPP2C98	GLYMA.17G034500	4	375	41 437.23	7.80	33.16	-0.208
GmPP2C99	GLYMA.17G038000	2	380	41 708.68	5.09	63.88	-0.022
GmPP2C100	GLYMA.17G052500	7	538	58 548.81	4.97	48.37	-0.374
GmPP2C101	GLYMA.17G105800	4	383	42 290.77	5.11	47.42	-0.344
GmPP2C102	GLYMA.17G153800	5	701	77 629.50	5.50	49.90	-0.447
GmPP2C103	GLYMA.17G218700	8	558	60 792.54	4.62	38.16	-0.208
GmPP2C104	GLYMA.17G221100	9	338	36 655.97	5.09	41.29	-0.188
GmPP2C105	GLYMA.17G230600	5	344	37 627.48	5.07	31.99	-0.240
GmPP2C106	GLYMA.17G243100	12	428	46 575.99	5.23	45.89	-0.294
GmPP2C107	GLYMA.18G035000	4	400	44 067.66	5.17	58.50	-0.443
GmPP2C108	GLYMA.18G061000	4	347	37 640.64	5.81	51.88	-0.222
GmPP2C109	GLYMA.18G180400	4	597	65 974.13	5.37	37.54	-0.349
GmPP2C110	GLYMA.18G208100	5	424	47 458.29	5.24	45.39	-0.473
GmPP2C111	GLYMA.18G242700	6	487	54 026.40	6.10	42.66	-0.578
GmPP2C112	GLYMA.18G283300	6	455	50 115.64	5.30	37.19	-0.325
GmPP2C113	GLYMA.19G069200	3	377	40 769.73	5.68	51.67	-0.354
GmPP2C114	GLYMA.19G147800	4	391	44 150.56	7.69	53.67	-0.278
GmPP2C115	GLYMA.19G176600	5	370	41 129.64	8.22	40.69	-0.272
GmPP2C116	GLYMA.19G222700	4	887	99 137.72	6.01	49.65	-0.516
GmPP2C117	GLYMA.19G230200	8	429	46 091.00	7.97	36.52	-0.092
GmPP2C118	GLYMA.19G230600	9	369	40 812.38	6.09	35.91	-0.243
GmPP2C119	GLYMA.20G106800	6	397	43 657.42	7.28	50.98	-0.270
GmPP2C120	GLYMA.20G119100	12	431	46 513.07	7.56	38.39	-0.235
GmPP2C121	GLYMA.20G131500	4	373	41 357.11	6.93	40.87	-0.180
GmPP2C122	GLYMA.20G207600	6	277	31 730.49	9.44	51.96	-0.467
GmPP2C123	GLYMA.20G238100	8	367	41 147.49	6.14	41.32	-0.389
GmPP2C124	GLYMA.20G238700	8	428	46 223.91	6.91	37.92	-0.181
GmPP2C125	GLYMA.20G244300	5	388	42 720.79	6.87	44.45	-0.222
GmPP2C126	GLYMA.20G248900	3	365	39 896.10	7.16	37.52	-0.239

大豆PP2C家族基因鉴定与响应盐胁迫的转录组分析

Identification of soybean PP2C family genes and transcriptome analysis in response to salt stress

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