玉米转录因子bZIP G亚家族基因的表达模式

doi:10.3969/j.issn.1004-1524.2022.02.02

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (2): 221-231.DOI: 10.3969/j.issn.1004-1524.2022.02.02

玉米转录因子bZIP G亚家族基因的表达模式

贾利强¹(), 赵秋芳², 陈曙², 丁波¹^,^*()

1.贵州师范学院生物科学学院,贵州贵阳 550018
2.中国热带农业科学院南亚热带作物研究所,广东湛江 524091

收稿日期:2021-05-19 出版日期:2022-02-25 发布日期:2022-03-02
作者简介:丁波,E-mail: lifescience2021@163.com
贾利强(1976—),男,河北石家庄人,博士,副教授,主要从事植物分子生物学研究。E-mail: liqiangj@163.com
通讯作者: 丁波
基金资助:
贵州师范学院博士项目(2019BS004)

Expression analysis of bZIP G subfamily genes in maize

JIA Liqiang¹(), ZHAO Qiufang², CHEN Shu², DING Bo¹^,^*()

1. School of Biological Sciences, Guizhou Education University, Guiyang 550018, China
2. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Guangdong, China

Received:2021-05-19 Online:2022-02-25 Published:2022-03-02
Contact: DING Bo

摘要/Abstract

摘要：

为探究不同逆境胁迫(盐、干旱、温度和硝态氮/铵态氮缺乏胁迫)对玉米ZmbZIP基因表达模式的影响,以玉米骨干自交系郑58为实验材料,设置200 mmol·L^-1 NaCl、20% PEG6000、4 ℃低温、硝态氮或铵态氮缺乏胁迫试验,探测其对bZIP家族基因G亚家族成员表达模式的影响。进化树分析结果显示,G亚家族成员进一步细分为3个亚组。qRT-PCR分析结果表明,15个ZmbZIPs基因具有不同的组织表达模式,表明这些基因在玉米生长发育中发挥不同的作用。人为模拟盐、干旱、低温和氮缺乏胁迫实验中,20个ZmbZIPs基因受不同逆境因子的广泛调控,预示ZmbZIP广泛参与调控玉米应答逆境胁迫响应途径。

关键词: bZIP转录因子, 逆境胁迫, 基因表达, 玉米

Abstract:

To explore the effect of various abiotic stresses on expression pattern of ZmbZIP, maize inbreed line Zheng58 was adopted as test materials, and five abiotic stresses (200 mmol·L ^-1 NaCl, 20% PEG6000, 4 ℃ low temperature, nitrate or ammonium deficiency stresses) were set, and expression of G group genes of bZIP family were systematically detected. Phylogenetic tree analysis showed that G group genes were subdivided into 3 subgroups. qRT-PCR analysis showed that 15 ZmbZIP genes had different expression pattern in various maize tissues, indicating their important roles during growth and development process in maize. Under different stresses of simulated salt, drought, low temperature and nitrogen deficiency, 20 ZmbZIP genes were widely regulated, indicating that most of those genes were involved in response to stress signaling pathway.

Key words: bZIP transcription factor, abiotic stresses, gene expression, maize

中图分类号:

S513

贾利强, 赵秋芳, 陈曙, 丁波. 玉米转录因子bZIP G亚家族基因的表达模式[J]. 浙江农业学报, 2022, 34(2): 221-231.

JIA Liqiang, ZHAO Qiufang, CHEN Shu, DING Bo. Expression analysis of bZIP G subfamily genes in maize[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 221-231.

图/表 8

参考文献 30

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基因名称 Gene name	基因号 Gene locus	上游引物 Forward primers (5'→3')	下游引物 Reverse primers (5'→3')
ZmbZIP2	Zm00001d028372	CATCCATCTGTAGCTCCAAGCCATC	CTGTCCCGTTTGGTAACTGCATAGG
ZmbZIP20	Zm00001d034571	GGCACGCTCAGATCAGAACTCGAC	ATGCTCAGAGTCGTCACAACGCTAG
ZmbZIP26	Zm00001d004897	CAGCAACGGGAAAGGAAGCCAC	GGCTGTGAAAACGCACTGGTTG
ZmbZIP39	Zm00001d005244	GGGAATGCTACCATTTTGCCACAAC	TTTCGCGCTTCAATTCCCTCTCATC
ZmbZIP40	Zm00001d040500	GCCAACACAGCAAGCTCTGCTC	CACGTTCATCCTGTATCCATTGCTC
ZmbZIP50	Zm00001d041142	CCCAGTGCTACAAATGTCGCGAAC	ACGAGCAGGCAGGAACAGCAAC
ZmbZIP51	Zm00001d043908	CCCTGGAATAGCGCCTGGTCATG	CTCCGCACTCAATGGGTTTACCAC
ZmbZIP67	Zm00001d043992	TCATCACGACCAGAGCCAAATCCA	ACCGCAATTGTTCCCGTGACGTTG
ZmbZIP73	Zm00001d052562	CCTATGCCAACAAATGGACATGCTG	CACTCTCACTCTCACTTCCACTCTC
ZmbZIP96	Zm00001d053967	CAATGAGCGTGGAGCCAGCCAA	TACATCGCTTGAGCCTTCTGCAC
ZmbZIP97	Zm00001d015118	CGTCTGCTCCATCCGCCAAC	AGAATCGCCATCTTCCTTCCCATC
ZmbZIP100	Zm00001d039065	GCATCGAGCCGCCGAAGTTG	TGCCTCCAGCACAGTTCCCATTG
ZmbZIP123	Zm00001d039206	CTCGTCCTGTCCGAGCACCTC	GTGAGTCCAACTACTGGCGGCTTAC
ZmbZIP133	Zm00001d019166	GGAAAGGAAGAAGGCGTACCTGAG	GCCTCTTCTGTTGTTGACAGTGGTG
ZmbZIP125	Zm00001d045288	GAACGGCCACACTTACCAGG	GATACGATCACGACGGCTCTAAACC
ZmbZIP136	Zm00001d047967	ATGGCGACGTTGGTGTGCCAC	GAGCGAGCCGTGATGGTGGTG
ZmbZIP138	Zm00001d046664	CAGCGTGGACCAGTTCTTCGAC	CATCGCCATCCACCTCCACCG
ZmbZIP140	Zm00001d024041	AGAACAACAACCGCAGACTCTCAAC	CCGCCTCCCACCTTCAACGAC
ZmbZIP142	Zm00001d024285	GCAACGCCTATACCGTTCTACCAG	CGGTGGATAATGGCAGGTCTGAG

基因名称 Gene name	基因号 Gene locus	上游引物 Forward primers (5'→3')	下游引物 Reverse primers (5'→3')
ZmbZIP2	Zm00001d028372	CATCCATCTGTAGCTCCAAGCCATC	CTGTCCCGTTTGGTAACTGCATAGG
ZmbZIP20	Zm00001d034571	GGCACGCTCAGATCAGAACTCGAC	ATGCTCAGAGTCGTCACAACGCTAG
ZmbZIP26	Zm00001d004897	CAGCAACGGGAAAGGAAGCCAC	GGCTGTGAAAACGCACTGGTTG
ZmbZIP39	Zm00001d005244	GGGAATGCTACCATTTTGCCACAAC	TTTCGCGCTTCAATTCCCTCTCATC
ZmbZIP40	Zm00001d040500	GCCAACACAGCAAGCTCTGCTC	CACGTTCATCCTGTATCCATTGCTC
ZmbZIP50	Zm00001d041142	CCCAGTGCTACAAATGTCGCGAAC	ACGAGCAGGCAGGAACAGCAAC
ZmbZIP51	Zm00001d043908	CCCTGGAATAGCGCCTGGTCATG	CTCCGCACTCAATGGGTTTACCAC
ZmbZIP67	Zm00001d043992	TCATCACGACCAGAGCCAAATCCA	ACCGCAATTGTTCCCGTGACGTTG
ZmbZIP73	Zm00001d052562	CCTATGCCAACAAATGGACATGCTG	CACTCTCACTCTCACTTCCACTCTC
ZmbZIP96	Zm00001d053967	CAATGAGCGTGGAGCCAGCCAA	TACATCGCTTGAGCCTTCTGCAC
ZmbZIP97	Zm00001d015118	CGTCTGCTCCATCCGCCAAC	AGAATCGCCATCTTCCTTCCCATC
ZmbZIP100	Zm00001d039065	GCATCGAGCCGCCGAAGTTG	TGCCTCCAGCACAGTTCCCATTG
ZmbZIP123	Zm00001d039206	CTCGTCCTGTCCGAGCACCTC	GTGAGTCCAACTACTGGCGGCTTAC
ZmbZIP133	Zm00001d019166	GGAAAGGAAGAAGGCGTACCTGAG	GCCTCTTCTGTTGTTGACAGTGGTG
ZmbZIP125	Zm00001d045288	GAACGGCCACACTTACCAGG	GATACGATCACGACGGCTCTAAACC
ZmbZIP136	Zm00001d047967	ATGGCGACGTTGGTGTGCCAC	GAGCGAGCCGTGATGGTGGTG
ZmbZIP138	Zm00001d046664	CAGCGTGGACCAGTTCTTCGAC	CATCGCCATCCACCTCCACCG
ZmbZIP140	Zm00001d024041	AGAACAACAACCGCAGACTCTCAAC	CCGCCTCCCACCTTCAACGAC
ZmbZIP142	Zm00001d024285	GCAACGCCTATACCGTTCTACCAG	CGGTGGATAATGGCAGGTCTGAG

基因ID Gene ID	基因名称 Gene name	长度 Length/bp	分子量 Molecular mass/ku	等电点 Isoelectric point	染色体 Chromosome	可变剪切 Splice variants
Zm00001d028372	ZmbZIP2	583	62.46	9.55	Chr1:32433751-32442505	17
Zm00001d034571	ZmbZIP20	356	37.47	7.59	Chr1:296596877-296600683	3
Zm00001d004897	ZmbZIP24	347	36.48	8.35	Chr2:147657998-147659916	1
Zm00001d005244	ZmbZIP26	371	38.75	7.16	Chr2:165521128-165527158	9
Zm00001d040500	ZmbZIP39	194	21.09	10.13	Chr3:47004098-47005326	1
Zm00001d041142	ZmbZIP40	382	40.63	8.41	Chr3:100232730-100240533	24
Zm00001d043908	ZmbZIP50	191	20.72	9.34	Chr3:213533138-213534279	1
Zm00001d043992	ZmbZIP51	340	37.03	8.8	Chr3:215850507-215866477	3
Zm00001d052562	ZmbZIP60	417	43.86	7.31	Chr4:193207888-193210831	1
Zm00001d053967	ZmbZIP67	314	33.62	6.17	Chr4:244414417-244418397	1
Zm00001d015118	ZmbZIP73	290	31.01	5.22	Chr5:76331375-76334353	1
Zm00001d039065	ZmbZIP96	377	40.12	7.51	Chr6:169491185-169496107	10
Zm00001d039206	ZmbZIP97	208	22.36	6.84	Chr6:172509105-172510659	1
Zm00001d019166	ZmbZIP100	379	39.68	6.88	Chr7:19967615-19972466	8
Zm00001d045288	ZmbZIP123	547	60.82	7.95	Chr9:18011858-18027409	1
Zm00001d047967	ZmbZIP133	423	45.11	9.94	Chr9:146611340-146618721	4
Zm00001d046664	ZmbZIP136	187	20.02	8.56	Chr10:5649486-5650525	1
Zm00001d024041	ZmbZIP138	282	29.93	7.12	Chr10:39073197-39078744	11
Zm00001d024285	ZmbZIP140	213	23.6	6.03	Chr10:61712124-61716615	1
Zm00001d025638	ZmbZIP142	273	30.15	8.45	Chr10:124742514-124747717	1

基因ID Gene ID	基因名称 Gene name	长度 Length/bp	分子量 Molecular mass/ku	等电点 Isoelectric point	染色体 Chromosome	可变剪切 Splice variants
Zm00001d028372	ZmbZIP2	583	62.46	9.55	Chr1:32433751-32442505	17
Zm00001d034571	ZmbZIP20	356	37.47	7.59	Chr1:296596877-296600683	3
Zm00001d004897	ZmbZIP24	347	36.48	8.35	Chr2:147657998-147659916	1
Zm00001d005244	ZmbZIP26	371	38.75	7.16	Chr2:165521128-165527158	9
Zm00001d040500	ZmbZIP39	194	21.09	10.13	Chr3:47004098-47005326	1
Zm00001d041142	ZmbZIP40	382	40.63	8.41	Chr3:100232730-100240533	24
Zm00001d043908	ZmbZIP50	191	20.72	9.34	Chr3:213533138-213534279	1
Zm00001d043992	ZmbZIP51	340	37.03	8.8	Chr3:215850507-215866477	3
Zm00001d052562	ZmbZIP60	417	43.86	7.31	Chr4:193207888-193210831	1
Zm00001d053967	ZmbZIP67	314	33.62	6.17	Chr4:244414417-244418397	1
Zm00001d015118	ZmbZIP73	290	31.01	5.22	Chr5:76331375-76334353	1
Zm00001d039065	ZmbZIP96	377	40.12	7.51	Chr6:169491185-169496107	10
Zm00001d039206	ZmbZIP97	208	22.36	6.84	Chr6:172509105-172510659	1
Zm00001d019166	ZmbZIP100	379	39.68	6.88	Chr7:19967615-19972466	8
Zm00001d045288	ZmbZIP123	547	60.82	7.95	Chr9:18011858-18027409	1
Zm00001d047967	ZmbZIP133	423	45.11	9.94	Chr9:146611340-146618721	4
Zm00001d046664	ZmbZIP136	187	20.02	8.56	Chr10:5649486-5650525	1
Zm00001d024041	ZmbZIP138	282	29.93	7.12	Chr10:39073197-39078744	11
Zm00001d024285	ZmbZIP140	213	23.6	6.03	Chr10:61712124-61716615	1
Zm00001d025638	ZmbZIP142	273	30.15	8.45	Chr10:124742514-124747717	1

玉米转录因子bZIP G亚家族基因的表达模式

Expression analysis of bZIP G subfamily genes in maize

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