浙江农业学报 ›› 2024, Vol. 36 ›› Issue (8): 1934-1944.DOI: 10.3969/j.issn.1004-1524.20240449
王建升(), 沈钰森, 虞慧芳, 盛小光, 宋蒙飞, 顾宏辉(
)
收稿日期:
2024-05-19
出版日期:
2024-08-25
发布日期:
2024-09-06
作者简介:
*顾宏辉,E-mail: guhh@zaas.ac.cn通讯作者:
顾宏辉
基金资助:
WANG Jiansheng(), SHEN Yusen, YU Huifang, SHENG Xiaoguang, SONG Mengfei, GU Honghui(
)
Received:
2024-05-19
Online:
2024-08-25
Published:
2024-09-06
Contact:
GU Honghui
摘要:
2018年以来,随着农业农村部“国家西兰花育种联合攻关”工作的实施,我国西兰花育种研究取得了重要进展。自主培育的西兰花品种在花球颜色、球形等重要性状方面取得了突破,自主培育品种的市场占有率由2018年的不足5%提升至2023年的35%左右,单倍体诱导技术广泛应用于育种实践,遗传转化和基因编辑技术取得突破,基因组与分子标记辅助育种技术在育种工作中发挥着重要作用。文章介绍了近年来我国在西兰花花球重要商品性状、育种技术、新品种选育等方面取得的重要进展。基于我国自主培育品种和对照品种的系统发育进化树分析结果,并结合主栽品种的历史更替,将我国西兰花品种分为3个历史阶段和17个进化群(簇),浙青、碧绿、美奥、台绿等最新育成品种位于进化树顶端。近几年西兰花育种主攻方向仍是早中熟、广适性品种,未来育种的方向是抗病、抗逆、适宜机械化生产,同时应从外观品质转向鲜食加工品质和功能营养品质。
中图分类号:
王建升, 沈钰森, 虞慧芳, 盛小光, 宋蒙飞, 顾宏辉. 中国西兰花育种研究进展[J]. 浙江农业学报, 2024, 36(8): 1934-1944.
WANG Jiansheng, SHEN Yusen, YU Huifang, SHENG Xiaoguang, SONG Mengfei, GU Honghui. Research progress of broccoli breeding in China[J]. Acta Agriculturae Zhejiangensis, 2024, 36(8): 1934-1944.
序号 Accession | 性状名称(其他名称) Trait name (other name) | 性状描述 Description of trait | 典型表型1 Typical phenotype | 参考文献2 Reference | ||||
---|---|---|---|---|---|---|---|---|
1 | 枯蕾(黄蕾) Withered bud (yellow bud) | 花蕾因失绿呈现黄色或白色或棕色的现象 The phenomenon of head buds appearing yellow, white, or brown due to greenness loss | ![]() | [ | ||||
2 | 满天星(猫眼、黄心) Scattered stars (cat eyes, yellow spots) | 球面呈现许多颜色明显比周围浅的圆形斑点, 似“星星”和“眼睛” The phenomenon of many circular spots appearing on the head surface with significantly lighter colors than the surrounding areas, look like “stars” and “eyes” | ![]() | [ | ||||
3 | 顶心 Top spot | 球面顶端呈现明显花蕾偏细小或颜色偏浅的现象 The phenomenon of obvious small or light colored buds appearing at the top of the head surface | ![]() | — | ||||
4 | 裂蕾 Cracking buds | 球面花蕾萼片裂开的现象 The phenomenon of sepals cracking of head buds | ![]() | [ | ||||
5 | 冒蕾 Welling buds | 花球表面有部分或个别花蕾明显高出周边花蕾导致 花蕾参差不齐的现象 The phenomenon where some or a few flower buds are significantly higher than the surrounding buds, resulting in uneven buds on head surface | ![]() | — | ||||
6 | 花蕾(蕾粒)大小 Bud (bead) size | 在收获时球面花蕾的大小 The size of head buds at harvest | ![]() | [ | ||||
7 | 花蕾(蕾粒)均匀度 (花蕾不匀) Bud uniformity | 花球收获时球面花蕾大小的一致性 Consistency in the size of buds on head surface at harvest | ![]() | [ | ||||
8 | 低温发紫 Turning-purple at low temperature (TPLT) | 低温或胁迫条件下,花球球面呈现紫色的现象 Under low temperature or stress conditions, the head surface exhibit purple | ![]() | [ | ||||
9 | 花蕾颜色与程度(花球 颜色与颜色程度) Bud color and degree (head color and degree ) | 花球(花蕾)颜色与深浅程度 Color and degree of head (buds) | ![]() | [ | ||||
10 | 球面不平整(表面凸起) Head planeness (head uniformity) | 花球球面因出现凸起或凹陷导致球面高低不平整 的现象 The uneven height of the head surface caused by protrusions or indentations | ![]() | [ | ||||
11 | 球形圆整性(球形圆整度) Sphericity index of head transection | 花球横切面呈现的形状,主要表现为圆形、五角形、 方形、边缘明显突出等 Shape of head transection, mainly manifested as circle, pentagon, square, and prominent edges | ![]() | — | ||||
12 | 球形指数(纵切面形状) Sphericity index (longitudinal section of head shape) | 花球纵切面呈现的形状。用花球纵径与横径之比 或者正弦值表示 Shape of head longitudinal section. It is represented by the ratio of the longitudinal diameter to the transverse diameter of the head or the sine value | ![]() | [ | ||||
13 | 花球松紧度(紧实度) Head tightness (head compaction) | 花球球面花蕾之间和小花球之间松散与紧实的程度 The degree of looseness and tightness among buds on head surface and small florets | ![]() | [ | ||||
14 | 侧球 Branching florets | 在主球下方有小的侧枝或侧球的现象 The phenomenon of small side branches or side florets below the main head | ![]() | [ | ||||
15 | 球茎空心 Hollow stem | 花球主茎内部出现中空的现象 The stem of main head appearing hollow | ![]() | [ | ||||
16 | 球面夹叶(夹小叶) Bracting (head-leaf) | 花球球面长出小叶子的现象 The phenomenon of leaves appearing on the head surface | ![]() | [ |
表1 西兰花花球主要外观商品性状特征
Table 1 Characteristics of main appearance commercial traits of broccoli head
序号 Accession | 性状名称(其他名称) Trait name (other name) | 性状描述 Description of trait | 典型表型1 Typical phenotype | 参考文献2 Reference | ||||
---|---|---|---|---|---|---|---|---|
1 | 枯蕾(黄蕾) Withered bud (yellow bud) | 花蕾因失绿呈现黄色或白色或棕色的现象 The phenomenon of head buds appearing yellow, white, or brown due to greenness loss | ![]() | [ | ||||
2 | 满天星(猫眼、黄心) Scattered stars (cat eyes, yellow spots) | 球面呈现许多颜色明显比周围浅的圆形斑点, 似“星星”和“眼睛” The phenomenon of many circular spots appearing on the head surface with significantly lighter colors than the surrounding areas, look like “stars” and “eyes” | ![]() | [ | ||||
3 | 顶心 Top spot | 球面顶端呈现明显花蕾偏细小或颜色偏浅的现象 The phenomenon of obvious small or light colored buds appearing at the top of the head surface | ![]() | — | ||||
4 | 裂蕾 Cracking buds | 球面花蕾萼片裂开的现象 The phenomenon of sepals cracking of head buds | ![]() | [ | ||||
5 | 冒蕾 Welling buds | 花球表面有部分或个别花蕾明显高出周边花蕾导致 花蕾参差不齐的现象 The phenomenon where some or a few flower buds are significantly higher than the surrounding buds, resulting in uneven buds on head surface | ![]() | — | ||||
6 | 花蕾(蕾粒)大小 Bud (bead) size | 在收获时球面花蕾的大小 The size of head buds at harvest | ![]() | [ | ||||
7 | 花蕾(蕾粒)均匀度 (花蕾不匀) Bud uniformity | 花球收获时球面花蕾大小的一致性 Consistency in the size of buds on head surface at harvest | ![]() | [ | ||||
8 | 低温发紫 Turning-purple at low temperature (TPLT) | 低温或胁迫条件下,花球球面呈现紫色的现象 Under low temperature or stress conditions, the head surface exhibit purple | ![]() | [ | ||||
9 | 花蕾颜色与程度(花球 颜色与颜色程度) Bud color and degree (head color and degree ) | 花球(花蕾)颜色与深浅程度 Color and degree of head (buds) | ![]() | [ | ||||
10 | 球面不平整(表面凸起) Head planeness (head uniformity) | 花球球面因出现凸起或凹陷导致球面高低不平整 的现象 The uneven height of the head surface caused by protrusions or indentations | ![]() | [ | ||||
11 | 球形圆整性(球形圆整度) Sphericity index of head transection | 花球横切面呈现的形状,主要表现为圆形、五角形、 方形、边缘明显突出等 Shape of head transection, mainly manifested as circle, pentagon, square, and prominent edges | ![]() | — | ||||
12 | 球形指数(纵切面形状) Sphericity index (longitudinal section of head shape) | 花球纵切面呈现的形状。用花球纵径与横径之比 或者正弦值表示 Shape of head longitudinal section. It is represented by the ratio of the longitudinal diameter to the transverse diameter of the head or the sine value | ![]() | [ | ||||
13 | 花球松紧度(紧实度) Head tightness (head compaction) | 花球球面花蕾之间和小花球之间松散与紧实的程度 The degree of looseness and tightness among buds on head surface and small florets | ![]() | [ | ||||
14 | 侧球 Branching florets | 在主球下方有小的侧枝或侧球的现象 The phenomenon of small side branches or side florets below the main head | ![]() | [ | ||||
15 | 球茎空心 Hollow stem | 花球主茎内部出现中空的现象 The stem of main head appearing hollow | ![]() | [ | ||||
16 | 球面夹叶(夹小叶) Bracting (head-leaf) | 花球球面长出小叶子的现象 The phenomenon of leaves appearing on the head surface | ![]() | [ |
图1 我国西兰花品种系统发育进化树分析 347个品种(387份样本)来自国家西兰花良种重大科研联合攻关组,14个(29份样本)为对照品种。外层不同颜色表示来自不同单位。
Fig.1 Phylogenetic tree of broccoli varieties from China The 347 varieties (387 samples) were from the National Joint Research Group for Major Scientific Research on Improved Varieties of Broccoli, and 14 (29 samples) were control varieties. The outer layers of different colors indicated that they come from different units.
[1] | 李占省, 刘玉梅, 方智远, 等. 我国青花菜产业发展现状、存在问题与应对策略[J]. 中国蔬菜, 2019(4): 1-5. |
LI Z S, LIU Y, FANG Z, et al. Current situation, issues and strategies of broccoli industry development in China[J]. Chinese Vegetable, 2019(4): 1-5. (in Chinese) | |
[2] | WANG J S, GU H H, YU H F, et al. Genotypic variation of glucosinolates in broccoli (Brassica oleracea var. italica) florets from China[J]. Food Chemistry, 2012, 133(3): 735-741. |
[3] | ClinicalTrials. Gov[DB/OL]. [2024-05-19]. https://www.clinicaltrials.gov/. |
[4] | LEE Y R, CHEN M, LEE J D, et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway[J]. Science, 2019, 364(6441): eaau0159. |
[5] | 李占省, 刘玉梅, 韩风庆, 等. “十三五”我国青花菜遗传育种研究进展[J]. 中国蔬菜, 2021(1): 33-40. |
LI Z S, LIU Y M, HAN F Q, et al. Research progress on broccoli genetic breeding during ‘The Thirteenth Five-year Plan’ in China[J]. China Vegetables, 2021(1): 33-40. (in Chinese with English abstract) | |
[6] | 浙江省农业农村厅种子管理站. 2023浙江西兰花新品种大会在台州召开[EB/OL]. (2023-12-22) [2024-05-19]. http://nynct.zj.gov.cn/art/2023/12/22/art_1630665_58956197.html. |
[7] | 方智远. 中国蔬菜育种学[M]. 北京: 中国农业出版社, 2017. |
[8] | STANSELL Z, BJÖRKMAN T. From landrace to modern hybrid broccoli: the genomic and morphological domestication syndrome within a diverse B. oleracea collection[J]. Horticulture Research, 2020, 7: 159. |
[9] | 盛小光, 赵振卿, 王建升, 等. 基于SSR标记的花椰菜和青花菜遗传多样性分析[J]. 植物遗传资源学报, 2019, 20(4): 949-959. |
SHENG X G, ZHAO Z Q, WANG J S, et al. Genetic diversity analysis of cauliflower and broccoli based on SSR markers[J]. Journal of Plant Genetic Resources, 2019, 20(4): 949-959. (in Chinese with English abstract) | |
[10] | 施俊生. 国家西兰花良种重大科研联合攻关进展及对策建议[J]. 浙江农业科学, 2019, 60(12): 2223-2225. |
SHI J S. Progress and suggestions on major scientific research joint tackling of national broccoli breeding[J]. Journal of Zhejiang Agricultural Sciences, 2019, 60(12): 2223-2225. (in Chinese with English abstract) | |
[11] | 全国植物新品种测试标准化技术委员会(SAC/TC 277). 植物新品种特异性、一致性和稳定性测试指南青花菜:NY/T 2757—2015[S]. 北京: 中国农业出版社, 2015. |
[12] | 苏英京. 临海西兰花优质高产机制与技术[M]. 北京: 中国农业出版社, 2011. |
[13] | 严继勇. 青花菜主要性状在自交后代中的遗传稳定性[J]. 中国蔬菜, 1999(6): 9-11. |
YAN J Y. Performance of broccoli main characters in different selfing generations[J]. China Vegetables, 1999(6): 9-11. (in Chinese with English abstract) | |
[14] | 刘二艳. 青花菜花球外观品质性状的遗传分析及分子标记研究[D]. 北京: 中国农业科学院, 2009. |
LIU E Y. Genetic dissection and SSR analysis of head appearance quality traits in broccoli (Brassica oleracer var. italica)[D]. Beijing: Chinese Academy of Agricultural Sciences, 2009. (in Chinese with English abstract) | |
[15] | YU H F, WANG J S, ZHAO Z Q, et al. Construction of a high-density genetic map and identification of loci related to hollow stem trait in broccoli (Brassic oleracea L. italica)[J]. Frontiers in Plant Science, 2019, 10: 45. |
[16] | LIU C Q, YAO X Q, LI G Q, et al. Identification of major loci and candidate genes for anthocyanin biosynthesis in broccoli using QTL-seq[J]. Horticulturae, 2021, 7(8): 246. |
[17] | YU H F, WANG J S, SHENG X G, et al. Construction of a high-density genetic map and identification of loci controlling purple sepal trait of flower head in Brassica oleracea L. italica[J]. BMC Plant Biology, 2019, 19(1): 228. |
[18] | YU H F, WANG J S, SHEN Y S, et al. A 43 bp-deletion in the F3'H gene reducing anthocyanins is responsible for keeping buds green at low temperatures in broccoli[J]. International Journal of Molecular Sciences, 2023, 24(14): 11391. |
[19] | WEN S Z, LI N, SONG S H, et al. Comparative transcriptome and metabolome analyses of broccoli germplasms with purple and green curds reveal the structural genes and transitional regulators regulating color formation[J]. International Journal of Molecular Sciences, 2023, 24(7): 6115. |
[20] | HAN F Q, HUANG J J, XIE Q, et al. Genetic mapping and candidate gene identification of BoGL5, a gene essential for cuticular wax biosynthesis in broccoli[J]. BMC Genomics, 2021, 22(1): 811. |
[21] | LIU Y X, WEI M, LIU Y M, et al. Functional characterization of BoGL5 by an efficient CRISPR/Cas9 genome editing system in broccoli[J]. Scientia Horticulturae, 2023, 319: 112136. |
[22] | 刘二艳, 刘玉梅, 方智远, 等. 青花菜花球‘荚叶’性状主基因+多基因遗传分析[J]. 园艺学报, 2009, 36(11): 1611-1618. |
LIU E Y, LIU Y M, FANG Z Y, et al. Genetic analysis of head-leaf traits using mixed major gene plus polygene inheritance model in Brassica oleracea L. var. italica planch[J]. Acta Horticulturae Sinica, 2009, 36(11): 1611-1618. (in Chinese) | |
[23] | 朱长志, 张志仙, 檀国印, 等. 青花菜主要农艺性状配合力遗传分析[J]. 江苏农业学报, 2016, 32(2): 430-436. |
ZHU C Z, ZHANG Z X, TAN G Y, et al. Genetic analysis of combining ability of major agronomic traits in Brassica oleracea L. var. italica[J]. Jiangsu Journal of Agricultural Sciences, 2016, 32(2): 430-436. (in Chinese with English abstract) | |
[24] | STANSELL Z, FARNHAM M, BJÖRKMAN T. Complex horticultural quality traits in broccoli are illuminated by evaluation of the immortal BolTBDH mapping population[J]. Frontiers in Plant Science, 2019, 10: 1104. |
[25] | 顾宏辉, 虞慧芳, 赵振卿, 等. 青花菜高二倍体率小孢子再生植株的培养方法: CN101617630B[P]. 2011-08-10. |
[26] | 高润红, 何婷, 郭桂梅, 等. 青花菜小孢子培养及再生体系的优化[J/OL]. 分子植物育种, (2023-12-01) [2024-06-12]. http://kns.cnki.net/kcms/detail/46.1068.S.20231130.1544.004.html. |
GAO R H, HE T, GUO G M, et al. Optimization of microspore culture and plant regeneration system of broccoli (Brassica oleracea var. italica)[J]. Molecular Plant Breeding, (2023-12-01) [2024-06-12]. http://kns.cnki.net/kcms/detail/46.1068.S.20231130.1544.004.html. (in Chinese with English abstract) | |
[27] | ZHAO X Y, YUAN K W, LIU Y X, et al. In vivo maternal haploid induction based on genome editing of DMP in Brassica oleracea[J]. Plant Biotechnology Journal, 2022, 20(12): 2242-2244. |
[28] | HAN F Q, ZHANG X L, LIU Y X, et al. One-step creation of CMS lines using a BoCENH3-based haploid induction system in Brassica crop[J]. Nature Plants, 2024, 10(4): 581-586. |
[29] | 唐征, 刘庆, 张小玲, 等. 甘蓝型油菜与青花菜种间杂种子房离体培养研究[J]. 中国农学通报, 2006, 22(10): 93-96. |
TANG Z, LIU Q, ZHANG X L, et al. In vitro culture of interspecific hybrid ovaries on Brassica napus and Brassica oleracea L.var. italica phenck[J]. Chinese Agricultural Science Bulletin, 2006, 22(10): 93-96. (in Chinese with English abstract) | |
[30] | 乔海云, 李菲, 张淑江, 等. 菜薹-青花菜种间三倍体AAC的合成及其生殖特性研究[J]. 园艺学报, 2012, 39(4): 655-660. |
QIAO H Y, LI F, ZHANG S J, et al. Synthesis of allotriploid (AAC)through hybridization between flowering Chinese cabbage and broccoli and the preliminary research on reproductive characters[J]. Acta Horticulturae Sinica, 2012, 39(4): 655-660. (in Chinese with English abstract) | |
[31] | 盛小光, 顾宏辉, 赵振卿, 等. 植物原生质体全能性表达及其在甘蓝类蔬菜育种上的应用[J]. 中国蔬菜, 2011(16): 1-8. |
SHENG X G, GU H H, ZHAO Z Q, et al. Totipotency expression of plant protoplast and its application in Brassica oleracea L. vegetable breeding[J]. China Vegetables, 2011(16): 1-8. (in Chinese with English abstract) | |
[32] | SHENG X G, ZHAO Z Q, YU H F, et al. Rapid alterations of DNA sequence and cytosine methylation induced by somatic hybridization between Brassica oleracea L. var. italica and Brassica nigra(L.) Koch[J]. Plant Cell, Tissue and Organ Culture(PCTOC), 2013, 115(3): 395-405. |
[33] | 戴永娟, 和兆荣, 胡靖锋, 等. 青花菜非对称体细胞杂交研究[J]. 湖南农业科学, 2012(5): 9-12. |
DAI Y J, HE Z R, HU J F, et al. Research on asymmetric somatic hybridization of broccoli[J]. Hunan Agricultural Sciences, 2012(5): 9-12. (in Chinese with English abstract) | |
[34] | SHENG X G, GU H H, YU H F, et al. An efficient shoot regeneration system and Agrobacterium-mediated transformation with codA gene in a doubled haploid line of broccoli[J]. Canadian Journal of Plant Science, 2016: 1-7. |
[35] | 邢苗苗, 许园园, 卢昱宇, 等. 通过遗传转化Rfo获得青花菜Ogura CMS恢复系[J]. 中国农业科学, 2023, 56(15): 2966-2976. |
XING M M, XU Y Y, LU Y Y, et al. Development of Ogura CMS restorers of broccoli via genetic transformation of Rfo[J]. Scientia Agricultura Sinica, 2023, 56(15): 2966-2976. (in Chinese with English abstract) | |
[36] | SHENG X G, YU H F, WANG J S, et al. Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea[J]. Frontiers in Plant Science, 2022, 13: 1021669. |
[37] | 黄文莉, 李香香, 周炆婷, 等. 利用CRISPR/Cas9技术靶向编辑青花菜BoZDS[J]. 生物技术通报, 2023, 39(2): 80-87. |
HUANG W L, LI X X, ZHOU W T, et al. Targeted editing of BoZDS in broccoli by CRISPR/Cas9 technology[J]. Biotechnology Bulletin, 2023, 39(2): 80-87. (in Chinese with English abstract) | |
[38] | XU F Y, SU T B, ZHANG X C, et al. Editing of ORF138 restores fertility of Ogura cytoplasmic male sterile broccoli via mitoTALENs[J]. Plant Biotechnology Journal, 2024, 22(5): 1325-1334. |
[39] | HAN F Q, LIU Y M, FANG Z Y, et al. Advances in genetics and molecular breeding of broccoli[J]. Horticulturae, 2021, 7(9): 280. |
[40] | BELSER C, ISTACE B, DENIS E, et al. Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps[J]. Nature Plants, 2018, 4(11): 879-887. |
[41] | LI X, WANG Y, CAI C C, et al. Large-scale gene expression alterations introduced by structural variation drive morphotype diversification in Brassica oleracea[J]. Nature Genetics, 2024, 56(3): 517-529. |
[42] | WU Q Y, MAO S X, HUANG H P, et al. Chromosome-scale reference genome of broccoli (Brassica oleracea var. italica Plenck) provides insights into glucosinolate biosynthesis[J]. Horticulture Research, 2024, 11(5): uhae063. |
[43] | HUANG J J, LIU Y M, HAN F Q, et al. Genetic diversity and population structure analysis of 161 broccoli cultivars based on SNP markers[J]. Horticultural Plant Journal, 2021, 7(5): 423-433. |
[44] | SHEN Y S, WANG J S, SHAW R K, et al. Development of GBTS and kasp panels for genetic diversity, population structure, and fingerprinting of a large collection of broccoli (Brassica oleracea L. var. italica) in China[J]. Frontiers in Plant Science, 2021, 12: 655254. |
[45] | LIU C Q, YAO X Q, LI G Q, et al. Development of novel markers and creation of non-anthocyanin and anthocyanin-rich broccoli (Brassica oleracea var. italica) cultivars[J]. Applied Sciences, 2022, 12(12): 6267. |
[46] | ZHANG X L, HAN F Q, LI Z S, et al. Map-based cloning and functional analysis of a major quantitative trait locus, BolC.Pb9.1, controlling clubroot resistance in a wild Brassica relative (Brassica macrocarpa)[J]. TAG Theoretical and Applied Genetics, 2024, 137(2): 41. |
[47] | 王建升, 沈钰森, 虞慧芳, 等. 西兰花浙青80的高效制种与纯度鉴定技术[J]. 浙江农业科学, 2023, 64(1): 75-78. |
WANG J S, SHEN Y S, YU H F, et al. Efficient seed production and purity identification technology of broccoli cultivar Zheqing 80[J]. Journal of Zhejiang Agricultural Sciences, 2023, 64(1): 75-78. (in Chinese with English abstract) | |
[48] | 武婷, 马存发, 赵辉, 等. 不同种衣剂对西兰花种子萌发和幼苗生长的影响[J]. 浙江农业科学, 2024, 65(5): 1148-1152. |
WU T, MA C F, ZHAO H, et al. Effects of different seed coating agents on the seed germination and seedling growth of Brassica oleracea[J]. Journal of Zhejiang Agricultural Sciences, 2024, 65(5): 1148-1152. (in Chinese with English abstract) |
[1] | 沈升法, 项超, 孟羽莎, 李兵, 吴列洪. 高光效甘薯品种浙薯86的选育、产量与品质特征[J]. 浙江农业学报, 2024, 36(7): 1469-1480. |
[2] | 潘志军, 吴小文, 吴晨阳, 程驭, 陈龙, 张晓红, 张进山, 周兵, 江波, 张文静, 车钊, 宋贺. 皖中不同类型再生稻品种产量与温光资源利用特征分析[J]. 浙江农业学报, 2024, 36(7): 1492-1501. |
[3] | 朱艳宇, 于文涛, 高水练, 吕水源, 王攀, 靳宛旻, 贵文静, 林浥, 叶乃兴. 福建安溪茶树种质资源遗传多样性与铁观音衍生品种遗传关系[J]. 浙江农业学报, 2024, 36(7): 1591-1601. |
[4] | 廖鹏飞, 李琼艳, 罗顺高, 刘敏, 朱红涛, 李继娅, 白红英, 陈海佺, 范永慧, 董占鹏. 家蚕素斑品种菁松的茶斑限性定向转育[J]. 浙江农业学报, 2024, 36(5): 1032-1041. |
[5] | 周贤桀, 程宝库, 张文斐. 英国《遗传技术(精准育种)法》评析及其对我国的启示[J]. 浙江农业学报, 2024, 36(5): 1199-1207. |
[6] | 王卓权, 林祯芃, 陈旭东, 钱斌, 翟荣荣, 叶胜海, 叶靖, 巫明明, 朱国富, 张小明. 不同原料糯米品种对绍兴黄酒品质的影响[J]. 浙江农业学报, 2024, 36(4): 773-779. |
[7] | 冷益丰, 罗樊, 陈从顺, 丁鑫, 蔡光泽. 基于GBS测序的全基因组SNP揭示大凉山玉米地方品种资源的亲缘关系与遗传分化[J]. 浙江农业学报, 2024, 36(1): 32-47. |
[8] | 王迪, 杨汉梅, 李阳倩, 贾梦婷, 邹亮, 杨帆. 苦荞麦“品、质、效、用”的多维评价及其活性成分高值化利用的研究进展[J]. 浙江农业学报, 2023, 35(8): 1960-1974. |
[9] | 孟羽莎, 王寅, 赖齐贤, 刘雷, 项超, 吴永华, 郑嫣然, 顾兴国, 方豪, 苗苗, 吴列洪, 汤勇. 甘薯近缘野生种ISBP分子标记的开发及其在遗传多样性分析和品种鉴定中的应用[J]. 浙江农业学报, 2023, 35(3): 489-498. |
[10] | 林小兵, 张鸿燕, 张秋梅, 周利军, 徐德胜, 郭乃嘉, 邱祥凤, 黄海平. 基于多指标的镉低积累水稻品种筛选[J]. 浙江农业学报, 2023, 35(11): 2507-2515. |
[11] | 姜友谊, 张成健, 韩少宇, 杨小冬, 杨贵军, 杨浩. 基于无人机三维点云的玉米植株自动计数研究[J]. 浙江农业学报, 2022, 34(9): 2032-2042. |
[12] | 万志前, 周贤桀. 品种权开放许可——理论阐释、规范构造与运行保障[J]. 浙江农业学报, 2022, 34(9): 2055-2065. |
[13] | 盛小光, 沈钰森, 虞慧芳, 王建升, 赵振卿, 顾宏辉. 花椰菜花球发育相关基因BoCAL的KASP标记开发和应用[J]. 浙江农业学报, 2022, 34(6): 1183-1192. |
[14] | 窦文卿, 柴春祥, 刘玥, 鲁晓翔. 质构技术快速辨别蜂蜜品种的研究[J]. 浙江农业学报, 2022, 34(5): 1073-1080. |
[15] | 李琳, 朱学明, 鲍坚东, 王教瑜, 林福呈. 基因编辑的“前世今生”[J]. 浙江农业学报, 2022, 34(5): 1091-1102. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||