草莓内生菌的分离筛选及其功能特性

doi:10.3969/j.issn.1004-1524.20240757

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (12): 2525-2534.DOI: 10.3969/j.issn.1004-1524.20240757

草莓内生菌的分离筛选及其功能特性

解雨佳¹(), 李镇江¹^,², 沙飞宇¹, 张琳³, 仝雅娜⁴, 乔长晟¹^,³, 曹伟锋¹^,^*()

1.天津科技大学生物工程学院,天津 300457
2.四川百川金开生物工程有限公司,四川成都 611130
3.天津慧智百川生物工程有限公司,天津 300456
4.天津市农业科学院林业果树研究所,天津 300384

收稿日期:2024-08-23 出版日期:2025-12-25 发布日期:2026-01-09
作者简介:解雨佳(1998—),女,河北唐山人,硕士研究生,主要从事内生菌研究。E-mail: xyj19981126@163.com
通讯作者: *曹伟锋,E-mail: weifcao@126.com

Isolation, screening, and functional characteristics of endophytes from strawberry

XIE Yujia¹(), LI Zhenjiang¹^,², SHA Feiyu¹, ZHANG Lin³, TONG Yana⁴, QIAO Changsheng¹^,³, CAO Weifeng¹^,^*()

1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
2. Sichuan Baichuan Jinkai Biological Engineering Co., Ltd., Chengdu 611130, China
3. Tianjin Huizhi Baichuan Biological Engineering Co., Ltd., Tianjin 300456, China
4. The Research Institute of Forestry and Pomology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China

Received:2024-08-23 Online:2025-12-25 Published:2026-01-09

摘要/Abstract

摘要：

为挖掘草莓内生菌的各种潜在功能,为后期开发内生菌菌剂提供新的菌种资源,以草莓品种红颜为对象,分离、筛选内生菌,采用平板法研究其促生能力,采用五点对峙法研究其对4种草莓常见病原菌[灰霉菌(Botrytis cinerea)、尖孢镰刀菌(Fusarium oxysporum)、胶孢炭疽菌(Colletotrichum gloeosporioides)、尖孢炭疽菌(Colletotrichum acutatum)]的拮抗能力,采用高通量测序分析内生菌的群落结构和多样性。结果显示,本研究共分离得到68株内生菌,其中,15株具有产吲哚乙酸的能力,48株具有产铁载体的能力,8株具有溶解无机磷的能力,23株具有溶解有机磷的能力,28株具有产纤维素酶的能力,33株具有产蛋白酶的能力,45株具有固氮的能力,47株具有解钾的能力。抑菌试验结果显示,14株内生菌对4种致病菌均表现出拮抗能力,另有6株内生菌至少对一种致病菌表现出拮抗能力。供试草莓的内生菌群在细菌门水平上主要包括变形菌门(Proteobacteria)、放线菌门(Actinobacteria),在真菌门水平上主要为子囊菌门(Ascomycota),在细菌属水平上主要包括链霉菌属(Streptomyces)、葡萄球菌属(Staphylococcus)、假单胞菌属(Pseudomonas),在真菌属水平上主要为Monographella。

关键词: 草莓, 内生菌, 促生功能, 拮抗功能, 病原菌

Abstract:

To explore the various potential functions of strawberry endophytes and provide new strain resources for the subsequent development of endophytic microbial agents, the strawberry cultivar Hongyan was used as the research object to isolate and screen endophytes. The plate method was employed to determine their growth-promoting abilities, while the five-point confrontation assay was used to evaluate their antagonistic activities against four common strawberry pathogens, namely Botrytis cinerea, Fusarium oxysporum, Colletotrichum gloeosporioides and Colletotrichum acutatum. High-throughput sequencing was applied to analyze the community structure and diversity of the endophytes. The results showed that a total of 68 endophytic strains were isolated. Among them, 15 strains had the ability to produce indole-3-acetic acid (IAA), 48 strains could produce siderophores, 8 strains exhibited inorganic phosphorus solubilization capacity, 23 strains had organic phosphorus solubilization ability, 28 strains were capable of producing cellulase, 33 strains could produce protease, 45 strains possessed nitrogen fixation capacity, and 47 strains could release potassium. The antibacterial test results indicated that 14 endophytic strains showed antagonistic activity against all four pathogenic fungi, and 6 other strains exhibited antagonistic effects against at least one pathogen. At the phylum level, the endophytic bacterial community of the tested strawberry was mainly composed of Proteobacteria and Actinobacteria, while the endophytic fungal community was dominated by Ascomycota. At the genus level, the dominant bacterial genera included Streptomyces, Staphylococcus, and Pseudomonas, and the dominant fungal genus was Monographella.

Key words: strawberry, endophyte, growth-promoting function, antagonistic function, pathogen

中图分类号:

S668.4
S144

解雨佳, 李镇江, 沙飞宇, 张琳, 仝雅娜, 乔长晟, 曹伟锋. 草莓内生菌的分离筛选及其功能特性[J]. 浙江农业学报, 2025, 37(12): 2525-2534.

XIE Yujia, LI Zhenjiang, SHA Feiyu, ZHANG Lin, TONG Yana, QIAO Changsheng, CAO Weifeng. Isolation, screening, and functional characteristics of endophytes from strawberry[J]. Acta Agriculturae Zhejiangensis, 2025, 37(12): 2525-2534.

图/表 6

参考文献 26

[1]	刘畅, 王晓, 李宪松, 等. 我国草莓生产态势及国内外比较分析[J]. 中国果树, 2023(7): 136-140.
	LIU C, WANG X, LI X S, et al. Strawberry production situation in China and comparative analysis at home and abroad[J]. China Fruits, 2023(7): 136-140. (in Chinese with English abstract)
[2]	STURZ A V, CHRISTIE B R, NOWAK J. Bacterial endophytes: potential role in developing sustainable systems of crop production[J]. Critical Reviews in Plant Sciences, 2000, 19(1): 1-30.
[3]	MARTINEZ-KLIMOVA E, RODRÍGUEZ-PEÑA K, SÁNCHEZ S. Endophytes as sources of antibiotics[J]. Biochemical Pharmacology, 2017, 134: 1-17.
[4]	ZHANG Z Y, LI J, ZHANG Z Q, et al. Tomato endophytic bacteria composition and mechanism of suppressiveness of wilt disease (Fusarium oxysporum)[J]. Frontiers in Microbiology, 2021, 12: 731764.
[5]	DE MOURA G G D, DE BARROS A V, MACHADO F, et al. The friend within: endophytic bacteria as a tool for sustainability in strawberry crops[J]. Microorganisms, 2022, 10(12): 2341.
[6]	LIU J M, WANG S S, ZHENG X, et al. Antimicrobial activity against phytopathogens and inhibitory activity on solanine in potatoes of the endophytic bacteria isolated from potato tubers[J]. Frontiers in Microbiology, 2020, 11: 570926.
[7]	GOODWIN P H. The endosphere microbiome of ginseng[J]. Plants, 2022, 11(3): 415.
[8]	陈向东. 植物内生菌是有待深入开发的资源宝库[J]. 微生物学通报, 2012, 39(2): 282.
	CHEN X D. Endophytic microorganisms are valuable resources worthy to be explored deeply[J]. Microbiology China, 2012, 39(2): 282. (in Chinese with English abstract)
[9]	姜道宏. 植物内生真菌及其展望[J]. 中国生物防治学报, 2015, 31(5): 742-749.
	JIANG D H. Endophytic fungi and their prospects[J]. Chinese Journal of Biological Control, 2015, 31(5): 742-749. (in Chinese with English abstract)
[10]	鲍敏, 康明浩. 植物内生菌研究发展现状[J]. 青海草业, 2011, 20(1): 21-25.
	BAO M, KANG M H. The summarize on research of endogenous bacteria[J]. Qinghai Prataculture, 2011, 20(1): 21-25. (in Chinese with English abstract)
[11]	康慧颖. 紫花苜蓿(Medicago sativa)内生菌的分离纯化及鉴定[D]. 哈尔滨: 哈尔滨师范大学, 2014.
	KANG H Y. Isolation and identification of endophytic bacterial isolates from alfalfa[D]. Harbin: Harbin Normal University, 2014. (in Chinese with English abstract)
[12]	肖蕾, 伍善东, 刘东华. 从油菜中分离到一株具多种生物学功能的优良内生细菌[J]. 农业与技术, 2021, 41(14): 5-8.
	XIAO L, WU S D, LIU D H. An excellent endophytic bacterium with multiple biological functions was isolated from rape[J]. Agriculture and Technology, 2021, 41(14): 5-8. (in Chinese with English abstract)
[13]	卢雨欣, 赵文娟, 白亚妮, 等. 耐盐碱番茄内生菌的筛选鉴定及功能研究[J]. 中国农学通报, 2023, 39(24): 50-58.
	LU Y X, ZHAO W J, BAI Y N, et al. Saline-alkali tolerant endophytic bacteria in tomato: screening, identification and functional study[J]. Chinese Agricultural Science Bulletin, 2023, 39(24): 50-58. (in Chinese with English abstract)
[14]	张平, 李朝阳, 赵清泉, 等. 生防细菌对油茶炭疽病病原菌的抑制作用[J]. 北京林业大学学报, 2020, 42(10): 107-116.
	ZHANG P, LI C Y, ZHAO Q Q, et al. Inhibition effects of biocontrol bacteria strains on the pathogen of Camellia oleifera anthracnose[J]. Journal of Beijing Forestry University, 2020, 42(10): 107-116. (in Chinese with English abstract)
[15]	卜春亚, 孙晔, 张天蔚, 等. 一株草莓根腐尖孢镰刀菌拮抗内生细菌的分离鉴定及抑菌特性[J]. 应用与环境生物学报, 2014, 20(2): 300-304.
	BU C Y, SUN Y, ZHANG T W, et al. Isolation and antagonistic character analysis of endophytic bacteria from the strawberry[J]. Chinese Journal of Applied and Environmental Biology, 2014, 20(2): 300-304. (in Chinese with English abstract)
[16]	GLICKMANN E, DESSAUX Y. A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria[J]. Applied and Environmental Microbiology, 1995, 61(2): 793-796.
[17]	雷平, 黄军, 黄彬彬, 等. 1株产铁载体辣椒内生细菌的分离鉴定及其促生长作用[J]. 激光生物学报, 2020, 29(4): 379-384.
	LEI P, HUANG J, HUANG B B, et al. Isolation, identification and growth promoting effect of a siderophore-producing endophytic bacterium from capscium[J]. Acta Laser Biology Sinica, 2020, 29(4): 379-384. (in Chinese with English abstract)
[18]	张敏. 猕猴桃内生菌次生代谢产物抗菌活性及应用研究[D]. 长沙: 湖南农业大学, 2019.
	ZHANG M. Antimicrobial activity and application of secondary metabolites from Actinidia chinensis endophytic bacteria[D]. Changsha: Hunan Agricultural University, 2019. (in Chinese with English abstract)
[19]	ROSENBLUETH M, MARTÍNEZ-ROMERO E. Bacterial endophytes and their interactions with hosts[J]. Molecular Plant-Microbe Interactions, 2006, 19(8): 827-837.
[20]	施宁雪, 张烨, 倪欢, 等. 健康草莓体内可分离培养微生物的多样性[J]. 江苏农业科学, 2021, 49(7): 130-135.
	SHI N X, ZHANG Y, NI H, et al. Diversity of isolatable and culturable endophytic microorganisms in healthy strawberry[J]. Jiangsu Agricultural Sciences, 2021, 49(7): 130-135. (in Chinese with English abstract)
[21]	胡应平, 林冬梅, 胡弘正, 等. 一株旱稻联合固氮菌株的分离鉴定及促生作用[J]. 西南农业学报, 2024, 37(4): 780-788.
	HU Y P, LIN D M, HU H Z, et al. Isolation, identification and growth-promoting characteristics of endophytic nitrogen-fixing bacteria in upland rice roots[J]. Southwest China Journal of Agricultural Sciences, 2024, 37(4): 780-788. (in Chinese with English abstract)
[22]	任明霞, 李静, 艾加敏, 等. 白刺花根瘤中分离细菌的物种多样性及其促生效应[J]. 微生物学报, 2024, 64(8): 2940-2954.
	REN M X, LI J, AI J M, et al. Species diversity and plant growth-promoting effects of bacteria isolated from the root nodules of Sophora davidii[J]. Acta Microbiologica Sinica, 2024, 64(8): 2940-2954. (in Chinese with English abstract)
[23]	李晴, 柴霜, 冯千禧, 等. 植物内生菌在农作物方面的应用探析[J]. 种子科技, 2021, 39(12): 8-9.
	LI Q, CHAI S, FENG Q X, et al. Application of endophytic bacteria in crops[J]. Seed Science & Technology, 2021, 39(12): 8-9. (in Chinese with English abstract)
[24]	赵杰, 田琼金, 聂蔓茹, 等. 红芸豆根腐病拮抗内生细菌的筛选和鉴定[J]. 山西农业科学, 2023, 51(10): 1226-1232.
	ZHAO J, TIAN Q J, NIE M R, et al. Screening and identification of antagonistic endophytic bacteria against root rot of red kidney bean[J]. Journal of Shanxi Agricultural Sciences, 2023, 51(10): 1226-1232. (in Chinese)
[25]	姜中宝, 黄宣雅, 唐振兴, 等. 具有抑菌和抗氧化活性紫金牛内生菌的筛选鉴定[J]. 杭州师范大学学报(自然科学版), 2023, 22(5): 497-504.
	JIANG Z B, HUANG X Y, TANG Z X, et al. Screening and identification of endophytes with antibacterial and antioxidant activities from Ardisia japonica[J]. Journal of Hangzhou Normal University(Natural Science Edition), 2023, 22(5): 497-504. (in Chinese with English abstract)
[26]	李方舟. 普洱茶树叶片内生细菌Bacillus velezensis FZ06的基因组测序及抗菌活性代谢产物研究[D]. 广州: 华南理工大学, 2020.
	LI F Z. Study on genome sequencing and antimicrobial metabolites produced by endophytic bacterium Bacillus velezensis FZ06 isolated from leaves of Camellia assamica[D]. Guangzhou: South China University of Technology, 2020. (in Chinese with English abstract)

编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	D/d					编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	D/d
编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	IC	IP	OP	CP	PP	编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	IC	IP	OP	CP	PP
NR1	9.79	-	-	1.77	-	-	-	1.13	PS4	-	+	+	2.25	-	-	-	-
NR2	19.31	-	-	1.38	-	-	-	3.14	PS5	-	+	+	-	-	-	2.09	1.76
NR3	5.31	-	-	2.08	-	-	-	2.85	PS6	19.50	-	+	-	-	1.29	-	-
NR4	-	+	+	2.33	-	-	-	-	PS7	-	+	+	1.67	-	1.19	6.96	-
NR5	-	+	+	1.41	-	-	-	-	PL1	-	-	-	2.67	-	-	1.73	1.98
NR6	-	+	+	-	-	-	-	1.88	PL2	-	+	+	1.83	-	-	-	2.42
NR7	-	-	-	-	-	2.11	-	3.14	PL3	-	-	-	-	-	-	1.26	1.50
NR8	-	+	+	1.43	-	1.51	2.50	2.08	PL4	-	-	+	-	-	1.16	-	1.54
NR9	-	-	-	-	-	2.00	-	2.29	PL5	17.50	+	-	-	-	1.47	1.43	-
NR10	-	+	+	1.67	-	-	4.67	3.00	PF1	-	+	-	2.10	-	-	-	4.38
NR11	-	+	+	-	-	1.44	2.56	2.87	PF2	-	+	+	1.64	-	-	-	-
NS1	25.41	-	+	1.47	1.23	3.91	-	-	GR1	-	+	+	2.21	-	-	-	-
NS2	-	+	-	-	-	-	-	1.79	GR2	-	+	+	4.09	1.32	-	-	1.14
NS3	0.84	+	+	1.63	-	-	4.10	1.95	GR3	-	+	+	1.91	1.18	-	-	-
NS4	5.89	+	+	1.60	1.83	5.83	-	1.47	GR4	-	-	-	-	1.45	1.45	1.90	-
NS5	8.36	+	+	-	-	1.20	-	-	GR5	16.61	+	+	1.42	-	-	-	-
NL1	-	+	+	1.25	-	-	-	2.56	GR6	88.05	+	+	2.67	-	-	-	-
NL2	-	+	+	1.56	-	-	-	-	GR7	-	-	-	-	-	-	2.50	2.83
NF1	34.93	+	+	2.36	-	-	-	-	GR8	-	+	-	-	-	1.46	5.79	-
NF2	34.17	+	-	2.27	-	-	-	-	GR9	-	-	-	1.89	-	1.54	1.67	-
NF3	-	+	+	1.38	-	-	2.61	-	GR10	-	-	-	1.60	-	1.48	1.62	3.52
NF4	-	+	+	1.50	-	-	1.86	2.00	GS1	-	+	+	1.20	-	-	-	-
PR1	-	-	+	2.00	-	-	-	2.21	GS2	-	+	+	1.75	1.93	5.07	-	1.17
PR2	-	+	+	1.47	-	-	2.75	-	GS3	-	+	+	1.35	1.32	3.63	5.07	1.39
PR3	-	-	+	1.12	-	-	1.08	-	GS4	-	-	-	-	-	-	3.73	2.38
PR4	-	+	+	-	-	-	1.06	-	GS5	1.22	-	-	-	-	1.84	2.91	-
PR5	-	+	+	-	-	1.88	-	2.36	GS6	-	+	+	2.29	-	-	5.07	-
PR6	-	-	+	1.75	-	-	2.59	2.66	GS7	-	-	+	1.73	-	-	-	-
PR7	-	-	-	-	-	1.19	-	1.91	GS8	-	+	+	-	-	-	-	-
PR8	-	+	+	1.46	-	-	-	-	GL1	-	+	+	1.33	1.36	2.64	4.00	-
PR9	16.08	+	+	1.78	-	1.27	-	2.48	GL2	-	+	+	1.63	-	1.84	-	2.24
PS1	-	+	+	1.38	-	-	-	-	GF1	-	+	-	1.37	-	-	-	-
PS2	-	-	+	1.67	-	-	2.75	-	GF2	-	+	+	2.44	-	-	-	-
PS3	-	+	+	1.60	-	-	1.80	1.79	GF3	-	-	-	2.00	-	-	2.18	-

编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	D/d					编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	D/d
编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	IC	IP	OP	CP	PP	编号 No.	Y_IAA/ (mg·L^-1)	FN	RP	IC	IP	OP	CP	PP
NR1	9.79	-	-	1.77	-	-	-	1.13	PS4	-	+	+	2.25	-	-	-	-
NR2	19.31	-	-	1.38	-	-	-	3.14	PS5	-	+	+	-	-	-	2.09	1.76
NR3	5.31	-	-	2.08	-	-	-	2.85	PS6	19.50	-	+	-	-	1.29	-	-
NR4	-	+	+	2.33	-	-	-	-	PS7	-	+	+	1.67	-	1.19	6.96	-
NR5	-	+	+	1.41	-	-	-	-	PL1	-	-	-	2.67	-	-	1.73	1.98
NR6	-	+	+	-	-	-	-	1.88	PL2	-	+	+	1.83	-	-	-	2.42
NR7	-	-	-	-	-	2.11	-	3.14	PL3	-	-	-	-	-	-	1.26	1.50
NR8	-	+	+	1.43	-	1.51	2.50	2.08	PL4	-	-	+	-	-	1.16	-	1.54
NR9	-	-	-	-	-	2.00	-	2.29	PL5	17.50	+	-	-	-	1.47	1.43	-
NR10	-	+	+	1.67	-	-	4.67	3.00	PF1	-	+	-	2.10	-	-	-	4.38
NR11	-	+	+	-	-	1.44	2.56	2.87	PF2	-	+	+	1.64	-	-	-	-
NS1	25.41	-	+	1.47	1.23	3.91	-	-	GR1	-	+	+	2.21	-	-	-	-
NS2	-	+	-	-	-	-	-	1.79	GR2	-	+	+	4.09	1.32	-	-	1.14
NS3	0.84	+	+	1.63	-	-	4.10	1.95	GR3	-	+	+	1.91	1.18	-	-	-
NS4	5.89	+	+	1.60	1.83	5.83	-	1.47	GR4	-	-	-	-	1.45	1.45	1.90	-
NS5	8.36	+	+	-	-	1.20	-	-	GR5	16.61	+	+	1.42	-	-	-	-
NL1	-	+	+	1.25	-	-	-	2.56	GR6	88.05	+	+	2.67	-	-	-	-
NL2	-	+	+	1.56	-	-	-	-	GR7	-	-	-	-	-	-	2.50	2.83
NF1	34.93	+	+	2.36	-	-	-	-	GR8	-	+	-	-	-	1.46	5.79	-
NF2	34.17	+	-	2.27	-	-	-	-	GR9	-	-	-	1.89	-	1.54	1.67	-
NF3	-	+	+	1.38	-	-	2.61	-	GR10	-	-	-	1.60	-	1.48	1.62	3.52
NF4	-	+	+	1.50	-	-	1.86	2.00	GS1	-	+	+	1.20	-	-	-	-
PR1	-	-	+	2.00	-	-	-	2.21	GS2	-	+	+	1.75	1.93	5.07	-	1.17
PR2	-	+	+	1.47	-	-	2.75	-	GS3	-	+	+	1.35	1.32	3.63	5.07	1.39
PR3	-	-	+	1.12	-	-	1.08	-	GS4	-	-	-	-	-	-	3.73	2.38
PR4	-	+	+	-	-	-	1.06	-	GS5	1.22	-	-	-	-	1.84	2.91	-
PR5	-	+	+	-	-	1.88	-	2.36	GS6	-	+	+	2.29	-	-	5.07	-
PR6	-	-	+	1.75	-	-	2.59	2.66	GS7	-	-	+	1.73	-	-	-	-
PR7	-	-	-	-	-	1.19	-	1.91	GS8	-	+	+	-	-	-	-	-
PR8	-	+	+	1.46	-	-	-	-	GL1	-	+	+	1.33	1.36	2.64	4.00	-
PR9	16.08	+	+	1.78	-	1.27	-	2.48	GL2	-	+	+	1.63	-	1.84	-	2.24
PS1	-	+	+	1.38	-	-	-	-	GF1	-	+	-	1.37	-	-	-	-
PS2	-	-	+	1.67	-	-	2.75	-	GF2	-	+	+	2.44	-	-	-	-
PS3	-	+	+	1.60	-	-	1.80	1.79	GF3	-	-	-	2.00	-	-	2.18	-

草莓内生菌的分离筛选及其功能特性

Isolation, screening, and functional characteristics of endophytes from strawberry

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