浙江农业学报

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外源亚精胺对干旱胁迫下白刺花幼苗生长的影响

  

  1. 1浙江自然博物馆,浙江 杭州 310012;2浙江省景宁畲族自治县林业总场大仰分场,浙江 丽水 323500;3中国农业大学 烟台研究院,山东 烟台 264670
  • 出版日期:2014-07-25 发布日期:2014-08-06

Effects of exogenous spermidine on the growth of Sophora davidii seedlings under drought stress#br#

  1. 1Zhejiang Museum of Natural History, Hangzhou 310012, China; 2Dayang Branch of Forestry Farm in Jingning She Autonomous County, Zhejiang Province, Lishui 323500, China; 3 China Agricultural University(Yantai), Yantai 264670, China
  • Online:2014-07-25 Published:2014-08-06

摘要:
摘要: 以白刺花(Sophora davidii)为试材,采用培养液栽培的方法研究了85% 聚乙二醇(PEG) 模拟的干旱胁迫下喷施外源亚精胺(Spd) 对白刺花幼苗生长的影响。结果表明, 外源亚精胺对干旱胁迫下白刺花叶片色素含量、叶片相对含水量、净光合速率和蒸腾速率均具有改善效果,在高浓度(T5和T6)时更为显著。总之,Spd浓度在0~050 mmol·L-1时,随着Spd浓度的增加,干旱胁迫下白刺花叶片的各生理指标数值逐渐升高: 叶绿素a和叶绿素b在Spd为010 mmol·L-1时含量最高,分别比对照组增加了1280%和875%;在Spd浓度为050 mmol·L-1时对净光合速率效果最明显,处理后1,3,5 d分别比T1增加了4735%,6265%和7464%;在Spd浓度为050 mmol·L-1时,处理后1,3,5 d叶片的蒸腾速率分别比对照组(CK)提高了2132%,1344%和1369%。

关键词: 干旱胁迫, 白刺花, 亚精胺, 叶片

Abstract: Sophora davidii was used as the test material, to study effects of exogenous spermidine on the growth of Sophora davidii seedlings under drought stress simulated by 85% polyethylene glycol (PEG). The results showed that the chlorophyll content, leaf relative water content, net photosynthetic rate and transpiration rate in Sophora davidii were improved by exogenous spermidine under drought stress, which was more significant at high concentrations (T5 and T6). In short, when Spd concentration was 000-050 mmol·L-1, the physiological index values gradually increased with increasing concentrations of Spd under drought stress. The concentration of chlorophyll—a and chlorophyll—b were the highest in the 010 mmol·L-1 Spd, which were 1280% and 875% higher than the control. The net photosynthetic rate increased by 4735%, 6265% and 7464% when compared with T1 on 1, 3 and 5 d in the treatment of 05 mmol·L-1 Spd; meanwhile, the transpiration rate increased by 2132%, 1344% and 1369% on 1, 3 and 5 d compared with the control group (CK).

Key words: drought stress, Sophora davidii, spermidine