浙江农业学报

• 动物科学 • 上一篇    下一篇

黑羽番鸭LPL基因多态性与肉质性状的关系

  

  1. (1江苏农牧科技职业学院,江苏 泰州 225300;2国家级水禽基因库,江苏 泰州 225300)
  • 出版日期:2014-09-25 发布日期:2014-10-11

Correlation of LPL gene polymorphism with meat quality in black Muscovy duck

  1. (1 Jiangsu Agri\|animal Husbandry Vocational College,Taizhou 225300, China;2 National Gene Bank of Waterfowl Resources, Taizhou 225300, China)
  • Online:2014-09-25 Published:2014-10-11

摘要: 采用PCR\|SSCP技术对黑羽番鸭脂蛋白脂酶(LPL)基因进行多态性研究,分析该基因与常规肉品质、肌肉营养成分等指标的相关性。结果表明:黑羽番鸭LPL基因第3内含子上发现了A4G突变,群体纯合度为05054,多态信息含量为03723,形成了EE、EF、FF 3种基因型。EF型母鸭腿肌失水率显著高于FF型(P<005,下同)。EF型公鸭腿肌中镁含量显著高于FF型。EE型公鸭胸肌中Ala含量显著高于EF型、FF型;FF型母鸭胸肌中Asp、His含量显著高于EF型;FF型公鸭腿肌中Ser、Pro含量显著高于EE型;EE型母鸭腿肌Lys含量显著高于EF型,EF型Cys、Val含量显著高于FF型。

关键词: 黑羽番鸭, LPL基因, 多态性, 肉质

Abstract: The polymorphism of Lipopretein lipase (LPL) gene was detected in black Muscovy duck by PCR\|SSCP, and the correlation between LPL gene polymorphism, routine meat quality, and muscle nutrients were analyzed. It was shown that the mutation of A\|G was discovered at the 4th site in intron 3. The homozygosity was 05054 in the whole population. The polymorphic information content was 03723. The mutations formed EE, EF and FF genotypes. The water loss rate of EF female duck was significantly (P<005) higher than that of FF female duck. The Mg content in leg muscle of the EF male duck was significantly (P<005) higher than that in the FF male duck. The Ala content in chest muscle of the EE male duck was significantly (P<005) higher than those of the EF and FF male duck. The Asp and His content in chest muscles of the FF female duck were significantly (P<005) higher than that of EF female duck. The contents of Ser and Pro in leg muscles of the FF male duck were significantly (P<005) higher than those of EE male duck. The Lys content in leg muscles of the EE female duck was significantly (P<005) higher than that of EF female duck,and the Cys and Val contents of EF female duck were significantly (P<005) higher than those of FF female duck.

Key words: black Muscovy duck, LPL gene, polymorphism, meat quality