关键词: 2, 3, 7, 8\, 四氯二苯\, p\, 二噁英, 谷草转氨酶, 谷丙转氨酶, 乳酸脱氢酶, 谷胱甘肽过氧化物酶, 谷胱甘肽S\, 转移酶, 总抗氧化能力

Abstract:  In order to evaluate the toxic effect of TCDD on fish liver， Jian carp were randomly divided into 6 groups （30 fish for each group） including a control group. The treated fish were injected with TCDD（01， 03， 06， 12， 24 μg·kg-1， respectively）. The control group had no TCDD injection， but was injected the same volume of DMSO. The blood and fish liver were collected at 24， 48， 72， 96， 120 h after injection， respectively， and the expression levels of LDH， GPT， GOT in Jian carp serum and GSH\|Px， GST， T\|AOC activity in liver homogenates were determined. It was shown that， after injection with TCDD for 72 h， GOT， GPT， LDH activities were significantly increased （P<001 or P<005）， while GST， GSH\|Px， T\|AOC activities were significantly decreased （P<001 or P<005）， indicating that TCDD could induce serious damage on fish liver and cause oxidative stress reaction in Jian carp after acute exposure.

Key words:  2, 3, 7, 8\, tetrachlorodibenzo\, p\, dioxin （TCDD）, GOT, GPT, LDH, GSH\, Px, GST, T\, AOC