碱蓬SgP5CS基因过表达提高拟南芥耐旱性

doi:10.3969/j.issn.1004-1524.2019.04.09

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (4): 572-578.DOI: 10.3969/j.issn.1004-1524.2019.04.09

碱蓬SgP5CS基因过表达提高拟南芥耐旱性

郭丹丹¹, 杨清华², 朱丹华², 金杭霞^2,*

1.浙江师范大学化学与生命科学学院,浙江金华321004;
2.浙江省农业科学院作物与核技术利用研究所,浙江杭州310021

收稿日期:2018-09-27 出版日期:2019-04-25 发布日期:2019-04-19
通讯作者: *金杭霞,E-mail: jinhangxia@126.com
作者简介:郭丹丹(1995-),女,河南洛阳人,硕士研究生,研究方向为生物化学与分子生物学。E-mail:guoddan@126.com
基金资助:
转基因生物新品种培育重大专项(2016ZX08004002-010)

Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis

GUO Dandan¹, YANG Qinghua², ZHU Danhua², JIN Hangxia^2,*

1.College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;
2. Institute of Crop Science and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-09-27 Online:2019-04-25 Published:2019-04-19

摘要/Abstract

摘要： 脯氨酸是植物中的渗透调节物质,Δ¹-吡咯啉-5-羧酸合成酶(P5CS)是其合成过程中重要的调控因子。为探究P5CS基因功能,克隆了碱蓬SgP5CS基因并导入拟南芥,使其在拟南芥中过表达,然后进行相关指标测定和耐旱性鉴定。结果显示,干旱胁迫诱导2周后,SgP5CS过表达的拟南芥植株具有较长的根系,同时游离脯氨酸的含量显著增加,丙二醛含量显著降低,表明SgP5CS基因在拟南芥中过表达能够增强拟南芥耐旱性。研究结果为深入了解碱蓬中SgP5CS基因的功能及其在植物中的抗旱机制奠定了基础。

关键词: 碱蓬, SgP5CS基因, 耐旱性, 拟南芥

Abstract: Δ¹-pyrroline-5-carboxylate synthetase (P5CS) is an important regulatory factor in synthesis of proline, an osmotic regulator in plants. SgP5CS gene of Suaeda salsa was cloned and introduced into Arabidopsis, the related physiological indexes and drought tolerance were determined, and the function of SgP5CS gene and regulation mechanism under drought stress were studied. The results showed that Arabidopsis with SgP5CS overexpression had long roots, while the content of free proline increased significantly and content of malondialdehyde decreased after two weeks of drought stress. It was found that overexpression of SgP5CS gene could enhance drought tolerance of transgenic Arabidopsis. These results laid a foundation to further study the SgP5CS gene function in Suaeda glauca, which might provide reference for the drought resistance mechanism in plants.

Key words: Suaeda salsa, SgP5CS gene, drought tolerance, Arabidopsis

中图分类号:

S184
Q496.1

郭丹丹, 杨清华, 朱丹华, 金杭霞. 碱蓬SgP5CS基因过表达提高拟南芥耐旱性[J]. 浙江农业学报, 2019, 31(4): 572-578.

GUO Dandan, YANG Qinghua, ZHU Danhua, JIN Hangxia. Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis[J]. , 2019, 31(4): 572-578.

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碱蓬SgP5CS基因过表达提高拟南芥耐旱性

Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis

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