不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响

doi:10.3969/j.issn.1004-1524.2021.02.11

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (2): 278-287.DOI: 10.3969/j.issn.1004-1524.2021.02.11

不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响

沈植国¹^,²(), 孙萌², 丁鑫², 程建明², 陈迪新³^,^*()

1.中南林业科技大学林学院,湖南长沙 410004
2.河南省林业科学研究院,河南郑州 450008
3.河南科技大学园艺与植物保护学院,河南洛阳 471023

收稿日期:2020-05-13 出版日期:2021-02-25 发布日期:2021-02-25
通讯作者: 陈迪新
作者简介:*陈迪新,E-mail: cdxdyp@163.com
沈植国(1977—),男,河南辉县人,博士研究生,高级工程师,主要从事经济林与园林花卉培育等研究。E-mail: 93611621@qq.com
基金资助:
河南省科技攻关项目(182102110458);河南省科技兴林项目(豫林计〔2018〕127号);河南省基本业务费(2018JB02-001)

Effects of different culture medium composition on pollen germination and pollen tube growth of 5 strains of Chimonanthus praecox

SHEN Zhiguo¹^,²(), SUN Meng², DING Xin², CHENG Jianming², CHEN Dixin³^,^*()

1. College of Forestry, Central South University of Forestry and Technology, Changsha 410004, China
2. Henan Academy of Forestry, Zhengzhou 450008, China
3. College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China

Received:2020-05-13 Online:2021-02-25 Published:2021-02-25
Contact: CHEN Dixin

摘要/Abstract

摘要：

以河南省许昌市3个类型5个品系的蜡梅花粉为试验材料,研究了不同培养基组分对蜡梅花粉萌发和花粉管生长的影响,以筛选出适宜不同类型蜡梅花粉萌发的最佳培养基,为生产实践中科学授粉和杂交育种提供理论依据。结果表明:聚乙二醇是蜡梅花粉萌发和花粉管生长的关键因素,当培养基中没有聚乙二醇时,花粉不能萌发;蔗糖不是蜡梅花粉离体萌发的必需因素,高浓度蔗糖还会抑制花粉萌发;硼酸和硝酸钙能够有效促进花粉萌发,在花粉萌发中起辅助作用;素心和红心类蜡梅花粉萌发和生长最佳培养基配方为PEG-4000 250 g·L^-1+Ca(NO₃)₂·4H₂O 10 mg·L^-1+H₃BO₃ 20 mg·L^-1,豫素1号、豫素2号、豫红1号花粉萌发率分别为25.38%、25.34%、23.46%,花粉管长度分别为302.31、297.64、285.68 μm;乔种蜡梅花粉萌发和生长最佳培养基配方为:PEG-4000 250 g·L^-1+Ca(NO₃)₂·4H₂O 5 mg·L^-1+H₃BO₃ 15 mg·L^-1,豫乔1号、豫乔2号花粉萌发率分别为24.43%和24.93%,花粉管长度分别为288.27 μm和286.37 μm。综合比较,5种花粉受培养基中相同因素影响的趋势相同,但在最适宜条件下,素心类型蜡梅的花粉活力较好,其萌发情况略好于其他2种类型。

关键词: 蜡梅, 花粉活力, 培养基组分, 花粉萌发, 花粉管长度

Abstract:

Five strains of Chimonanthus praecox fresh pollens collected from Xuchang City, Henan Province were used as materials to test and screen the best culture medium for pollen germination of C. praecox. Effects of different culture medium composition on pollen germination and pollen tube growth of C. praecox were investigated with in-vitro culture method. The results showed that PEG-4000 was the key factor for pollen in-vitro germination and its tube growth of C. praecox, and the pollen failed to germinate when there was no PEG-4000 in the medium. On the contrary, sucrose was unnecessary factor for pollen in-vitro germination and high concentration sucrose inhibited pollen germination; While boric acid and calcium nitrate could promote pollen germination effectively, which played an auxiliary role in pollen germination. The optimum medium formulation for C. praecox var. concolor and C. praecox var. patens was 250 g·L^-1 PEG-4000+10 mg·L^-1 Ca (NO₃)₂·4H₂O+20 mg·L^-1 H₃BO₃, the pollen germination rates of Yusu No.1, Yusu No.2 and Yuhong No.1 were 25.38%, 25.34% and 23.46%, respectively, and their pollen tube lengths were 302.31 μm, 297.64 μm and 285.68 μm, respectively. The optimum medium formulation for C. praecox var. intermedius was 250 g·L^-1 PEG-4000+5 mg·L^-1 Ca(NO₃)₂·4H₂O+15 mg·L^-1 H₃BO₃, the pollen germination rates of Yuqiao No.1 and Yuqiao No.2 were 24.43% and 24.93%, their pollen tube lengths were 288.27 μm and 286.37 μm, respectively. The influence trend of the same factors in the culture medium was the same for the five kinds of pollen, but under the most suitable conditions, the pollen vitality of C. praecox var. concolor was better, and its germination rate was slightly higher than that of the other two types.

Key words: Chimonanthus praecox, pollen viability, culture medium composition, pollen germination, pollen tube length

中图分类号:

S685.99

沈植国, 孙萌, 丁鑫, 程建明, 陈迪新. 不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响[J]. 浙江农业学报, 2021, 33(2): 278-287.

SHEN Zhiguo, SUN Meng, DING Xin, CHENG Jianming, CHEN Dixin. Effects of different culture medium composition on pollen germination and pollen tube growth of 5 strains of Chimonanthus praecox[J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 278-287.

图/表 6

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编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	花粉萌发率Pollen germination rate/%								花粉管长度Pollen tube length/μm
编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	豫素1号 Yusu No.1	豫素2号 Yusu No.2			豫乔1号 Yuqiao No.1	豫乔2号 Yuqiao No.2	豫红1号 Yuhong No.1		豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1
1	0	0	0	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
2	0	5	5	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
3	0	10	10	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
4	0	15	15	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
5	0	20	20	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
6	150	0	5	12.16 ±1.37 j	11.47 ±0.96 i			10.46 ±1.36 i	9.42 ±0.55 i	10.56 ±0.76 h		136.75 ±9.68 j		134.24 ±11.25 j		137.98 ±9.65 h		142.17 ±14.23 i		127.98 ±13.69 h
7	150	5	10	13.88 ±1.26 fghij	13.24 ±0.45 ghj			12.03 ±0.61 ghi	12.43 ±0.61 h	12.13 ±0.38 fgh		192.56 ±19.31 fgh		187.49 ±13.23 efgh		180.74 ±18.36 ef		180.34 ±18.39 fg		180.24 ±19.17 ef
8	150	10	15	16.14 ±1.45 ef	15.33 ±0.78 fg			13.58 ±1.23 efg	12.58 ±0.61 gh	13.68 ±0.51 def		199.45 ±12.62 efg		198.45 ±19.52 defg		178.35 ±21.20 ef		178.55 ±18.21 fg		178.35 ±16.98 ef
9	150	15	20	12.88 ±1.39 ij	11.84 ±1.29 i			14.84 ±1.55 e	14.14 ±0.58 fgh	14.84 ±0.53 d		220.66 ±15.36 cde		210.34 ±16.32 cde		201.25 ±23.03 cde		211.25 ±20.97cd		211.25 ±22.35 cd
10	150	20	0	13.7 ±1.41 ghij	12.6 ±0.82 hi			14.27 ±1.82 efg	14.67 ±0.79 fg	14.27 ±0.64 def		188.66 ±17.23 fgh		184.32 ±18.26 efghi		178.34 ±15.96 ef		168.34 ±15.98 gh		178.34 ±19.87 ef
11	200	0	10	14.55 ±1.23 fghi	13.69 ±1.36 ghj			11.35 ±1.47 hi	10.35 ±0.32 i	11.25 ±0.43 gh		177.46 ±12.96 ghi		171.64 ±18.33 ghi		168.59 ±21.03 fg		167.09 ±17.23 ghi		168.59 ±15.36 fg
12	200	5	15	14.62 ±1.33 fghi	14.14 ±1.41 fgh			13.87 ±1.61 efg	12.47 ±0.59 h	13.87 ±0.97 def		202.12 ±19.36 defg		203.14 ±21.14 def		201.54 ±23.02 cde		201.14 ±19.91 def		201.54 ±21.03 cde
13	200	10	20	15.73 ±1.61 fg	15.97 ±0.93 ef			13.28 ±1.29 efgh	13.38 ±0.43 gh	13.28 ±0.58 defg		228.68 ±18.75 cd		224.64 ±22.03 cd		215.69 ±20.09 cd		212.69 ±24.58 cd		215.69 ±22.53 cd
14	200	15	0	14.26 ±1.29 fghij	13.47 ±0.44 ghj			13.72 ±1.36 efg	14.72 ±0.37 fg	13.72 ±0.71 def		175.46 ±15.78 ghi		172.64 ±15.28 ghi		169.34 ±16.08 fg		169.34 ±18.39 gh		169.34 ±15.12 fg
15	200	20	5	13.52 ±1.38 ghij	12.89 ±1.36 hi			12.65 ±1.28 efgh	12.45 ±0.38 h	12.65 ±0.56 defgh		244.66 ±21.32 c		234.31 ±18.36 c		225.12 ±25.03 c		235.12 ±17.83 bc		225.12 ±17.61 c
16	250	0	15	20.61 ±1.63 c	20.24 ±1.63 c			21.34 ±1.58 c	21.24 ±1.63c	21.34 ±1.29 b		177.53 ±11.11 ghi		169.47 ±11.30 hi		254.26 ±21.01 b		253.26 ±21.36 b		254.26 ±22.30 b
17	250	5	20	22.74 ±1.54 b	22.67 ±0.51b			22.25 ±1.62 bc	22.05 ±1.41 bc	22.25 ±1.67 ab		220.39 ±20.03 cde		224.43 ±21.03 cd		218.95 ±19.86 cd		208.95 ±24.02 cde		218.95 ±18.37 cd
18	250	10	0	20.45 ±1.28 c	19.41 ±1.38 cd			18.45 ±1.61 d	18.43 ±0.71 d	18.45 ±1.46 c		166.78 ±12.93 hi		167.64 ±9.52 hi		253.91 ±19.86 b		233.14 ±21.29 bc		253.91 ±21.09 b
19	250	15	5	25.02 ±0.36 a	24.93 ±0.91 a			24.43 ±1.41 a	24.93 ±1.36 a	22.43 ±0.83 ab		277.55 ±21.02 b		270.43 ±18.41 b		288.27 ±27.89 a		286.37 ±18.23 a		268.27 ±17.52 ab
编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	花粉萌发率Pollen germination rate/%									花粉管长度Pollen tube length/μm
编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	豫素1号 Yusu No.1		豫素2号 Yusu No.2	豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1		豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2	豫红1号 Yuhong No.1
20	250	20	10	25.38 ±1.54 a		25.34 ±1.73 a	23.46 ±1.33 ab		23.26 ±1.42ab		23.46 ±0.75 a		302.31 ±19.45 a		297.64 ±23.39 a		285.68 ±23.93 a		235.68 ±13.59 bc	285.68 ±19.36 a
21	300	0	20	16.06 ±1.19 ef		15.31 ±1.36 fg	14.47 ±1.28 ef		14.47 ±0.53 fgh		14.47 ±0.71 de		184.19 ±18.57 fgh		180.34 ±17.19 fghi		178.25 ±15.36 ef		176.35 ±13.28 fg	178.25 ±11.25 ef
22	300	5	0	13.11 ±1.61 hij		13.41 ±0.98 ghj	12.56 ±1.19 fgh		12.5 ±0.45 h		12.56 ±0.32 efgh		155.48 ±12.36 ij		158.14 ±13.07 ij		149.16 ±12.57 gh		149.46 ±15.83 hi	149.16 ±9.18 gh
23	300	10	5	15.44 ±1.38 fgh		14.67 ±0.61 fgh	13.68 ±1.12 efg		12.68 ±0.51 gh		13.68 ±0.48 def		189.72 ±12.13 fgh		184.69 ±15.83 efghi		183.99 ±17.54 ef		183.89 ±16.26 efg	183.99 ±15.63 ef
24	300	15	10	19.87 ±1.02 cd		19.41 ±0.95 cd	18.75 ±1.61 d		15.75 ±0.98 ef		18.75 ±0.87 c		208.1 ±10.39 def		207.24 ±11.20 def		200.45 ±17.28 cde		200.15 ±14.26 def	200.45 ±16.43 cde
25	300	20	15	18.05 ±1.83 de		17.68 ±0.94 de	17.25 ±1.32 d		17.15 ±1.63 de		17.25 ±0.62 c		271.66 ±22.53 b		263.14 ±23.06 b		195.37 ±16.32 def		194.47 ±19.12 defg	195.37 ±15.39 def

编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	花粉萌发率Pollen germination rate/%								花粉管长度Pollen tube length/μm
编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	豫素1号 Yusu No.1	豫素2号 Yusu No.2			豫乔1号 Yuqiao No.1	豫乔2号 Yuqiao No.2	豫红1号 Yuhong No.1		豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1
1	0	0	0	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
2	0	5	5	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
3	0	10	10	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
4	0	15	15	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
5	0	20	20	0 k	0 j			0 j	0 j	0 i		0 k		0 k		0 i		0 j		0 i
6	150	0	5	12.16 ±1.37 j	11.47 ±0.96 i			10.46 ±1.36 i	9.42 ±0.55 i	10.56 ±0.76 h		136.75 ±9.68 j		134.24 ±11.25 j		137.98 ±9.65 h		142.17 ±14.23 i		127.98 ±13.69 h
7	150	5	10	13.88 ±1.26 fghij	13.24 ±0.45 ghj			12.03 ±0.61 ghi	12.43 ±0.61 h	12.13 ±0.38 fgh		192.56 ±19.31 fgh		187.49 ±13.23 efgh		180.74 ±18.36 ef		180.34 ±18.39 fg		180.24 ±19.17 ef
8	150	10	15	16.14 ±1.45 ef	15.33 ±0.78 fg			13.58 ±1.23 efg	12.58 ±0.61 gh	13.68 ±0.51 def		199.45 ±12.62 efg		198.45 ±19.52 defg		178.35 ±21.20 ef		178.55 ±18.21 fg		178.35 ±16.98 ef
9	150	15	20	12.88 ±1.39 ij	11.84 ±1.29 i			14.84 ±1.55 e	14.14 ±0.58 fgh	14.84 ±0.53 d		220.66 ±15.36 cde		210.34 ±16.32 cde		201.25 ±23.03 cde		211.25 ±20.97cd		211.25 ±22.35 cd
10	150	20	0	13.7 ±1.41 ghij	12.6 ±0.82 hi			14.27 ±1.82 efg	14.67 ±0.79 fg	14.27 ±0.64 def		188.66 ±17.23 fgh		184.32 ±18.26 efghi		178.34 ±15.96 ef		168.34 ±15.98 gh		178.34 ±19.87 ef
11	200	0	10	14.55 ±1.23 fghi	13.69 ±1.36 ghj			11.35 ±1.47 hi	10.35 ±0.32 i	11.25 ±0.43 gh		177.46 ±12.96 ghi		171.64 ±18.33 ghi		168.59 ±21.03 fg		167.09 ±17.23 ghi		168.59 ±15.36 fg
12	200	5	15	14.62 ±1.33 fghi	14.14 ±1.41 fgh			13.87 ±1.61 efg	12.47 ±0.59 h	13.87 ±0.97 def		202.12 ±19.36 defg		203.14 ±21.14 def		201.54 ±23.02 cde		201.14 ±19.91 def		201.54 ±21.03 cde
13	200	10	20	15.73 ±1.61 fg	15.97 ±0.93 ef			13.28 ±1.29 efgh	13.38 ±0.43 gh	13.28 ±0.58 defg		228.68 ±18.75 cd		224.64 ±22.03 cd		215.69 ±20.09 cd		212.69 ±24.58 cd		215.69 ±22.53 cd
14	200	15	0	14.26 ±1.29 fghij	13.47 ±0.44 ghj			13.72 ±1.36 efg	14.72 ±0.37 fg	13.72 ±0.71 def		175.46 ±15.78 ghi		172.64 ±15.28 ghi		169.34 ±16.08 fg		169.34 ±18.39 gh		169.34 ±15.12 fg
15	200	20	5	13.52 ±1.38 ghij	12.89 ±1.36 hi			12.65 ±1.28 efgh	12.45 ±0.38 h	12.65 ±0.56 defgh		244.66 ±21.32 c		234.31 ±18.36 c		225.12 ±25.03 c		235.12 ±17.83 bc		225.12 ±17.61 c
16	250	0	15	20.61 ±1.63 c	20.24 ±1.63 c			21.34 ±1.58 c	21.24 ±1.63c	21.34 ±1.29 b		177.53 ±11.11 ghi		169.47 ±11.30 hi		254.26 ±21.01 b		253.26 ±21.36 b		254.26 ±22.30 b
17	250	5	20	22.74 ±1.54 b	22.67 ±0.51b			22.25 ±1.62 bc	22.05 ±1.41 bc	22.25 ±1.67 ab		220.39 ±20.03 cde		224.43 ±21.03 cd		218.95 ±19.86 cd		208.95 ±24.02 cde		218.95 ±18.37 cd
18	250	10	0	20.45 ±1.28 c	19.41 ±1.38 cd			18.45 ±1.61 d	18.43 ±0.71 d	18.45 ±1.46 c		166.78 ±12.93 hi		167.64 ±9.52 hi		253.91 ±19.86 b		233.14 ±21.29 bc		253.91 ±21.09 b
19	250	15	5	25.02 ±0.36 a	24.93 ±0.91 a			24.43 ±1.41 a	24.93 ±1.36 a	22.43 ±0.83 ab		277.55 ±21.02 b		270.43 ±18.41 b		288.27 ±27.89 a		286.37 ±18.23 a		268.27 ±17.52 ab
编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	花粉萌发率Pollen germination rate/%									花粉管长度Pollen tube length/μm
编号 Serial number	PEG-4000/ (g·L^-1)	H₃BO₃/ (mg·L^-1)	Ca(NO₃)₂· 4H₂O/ (g·L^-1)	豫素1号 Yusu No.1		豫素2号 Yusu No.2	豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1		豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2	豫红1号 Yuhong No.1
20	250	20	10	25.38 ±1.54 a		25.34 ±1.73 a	23.46 ±1.33 ab		23.26 ±1.42ab		23.46 ±0.75 a		302.31 ±19.45 a		297.64 ±23.39 a		285.68 ±23.93 a		235.68 ±13.59 bc	285.68 ±19.36 a
21	300	0	20	16.06 ±1.19 ef		15.31 ±1.36 fg	14.47 ±1.28 ef		14.47 ±0.53 fgh		14.47 ±0.71 de		184.19 ±18.57 fgh		180.34 ±17.19 fghi		178.25 ±15.36 ef		176.35 ±13.28 fg	178.25 ±11.25 ef
22	300	5	0	13.11 ±1.61 hij		13.41 ±0.98 ghj	12.56 ±1.19 fgh		12.5 ±0.45 h		12.56 ±0.32 efgh		155.48 ±12.36 ij		158.14 ±13.07 ij		149.16 ±12.57 gh		149.46 ±15.83 hi	149.16 ±9.18 gh
23	300	10	5	15.44 ±1.38 fgh		14.67 ±0.61 fgh	13.68 ±1.12 efg		12.68 ±0.51 gh		13.68 ±0.48 def		189.72 ±12.13 fgh		184.69 ±15.83 efghi		183.99 ±17.54 ef		183.89 ±16.26 efg	183.99 ±15.63 ef
24	300	15	10	19.87 ±1.02 cd		19.41 ±0.95 cd	18.75 ±1.61 d		15.75 ±0.98 ef		18.75 ±0.87 c		208.1 ±10.39 def		207.24 ±11.20 def		200.45 ±17.28 cde		200.15 ±14.26 def	200.45 ±16.43 cde
25	300	20	15	18.05 ±1.83 de		17.68 ±0.94 de	17.25 ±1.32 d		17.15 ±1.63 de		17.25 ±0.62 c		271.66 ±22.53 b		263.14 ±23.06 b		195.37 ±16.32 def		194.47 ±19.12 defg	195.37 ±15.39 def

因素 Factor	花粉萌发率Pollen germination rate										花粉管长度Pollen tube length
	豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1		豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1
	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F
PEG	349.57	128.36^*	337.74	105.18^*	318.35	139.03^*	312.70	120.32^*	309.25	150.52^*	43540.03	43.75^*	42074.37	45.23^*	47004.70	107.66^*	43297.28	84.21^*	46041.28	94.68^*
H₃BO₃	2.87	1.05	2.67	0.83	7.38	3.22	8.03	3.09	5.79	2.82	3163.21	3.18	2881.02	3.10	850.06	1.95	651.09	1.27	886.70	1.82
Ca(NO₃)₂	3.97	1.46	4.40	1.37	1.90	0.83	0.76	0.29	2.47	1.20	1217.20	1.22	1038.17	1.12	257.68	0.59	485.19	0.94	236.53	0.49
R²	0.978	0.973	0.979	0.976	0.981	0.941	0.943	0.973	0.966	0.970

因素 Factor	花粉萌发率Pollen germination rate										花粉管长度Pollen tube length
	豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1		豫素1号 Yusu No.1		豫素2号 Yusu No.2		豫乔1号 Yuqiao No.1		豫乔2号 Yuqiao No.2		豫红1号 Yuhong No.1
	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F	MS	F
PEG	349.57	128.36^*	337.74	105.18^*	318.35	139.03^*	312.70	120.32^*	309.25	150.52^*	43540.03	43.75^*	42074.37	45.23^*	47004.70	107.66^*	43297.28	84.21^*	46041.28	94.68^*
H₃BO₃	2.87	1.05	2.67	0.83	7.38	3.22	8.03	3.09	5.79	2.82	3163.21	3.18	2881.02	3.10	850.06	1.95	651.09	1.27	886.70	1.82
Ca(NO₃)₂	3.97	1.46	4.40	1.37	1.90	0.83	0.76	0.29	2.47	1.20	1217.20	1.22	1038.17	1.12	257.68	0.59	485.19	0.94	236.53	0.49
R²	0.978	0.973	0.979	0.976	0.981	0.941	0.943	0.973	0.966	0.970

不同培养基组分对5个蜡梅品系花粉萌发和花粉管生长的影响

Effects of different culture medium composition on pollen germination and pollen tube growth of 5 strains of Chimonanthus praecox

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