高精饲粮中添加苦豆子对羔羊生长与血液生化指标的影响

doi:10.3969/j.issn.1004-1524.2022.05.04

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (5): 908-914.DOI: 10.3969/j.issn.1004-1524.2022.05.04

高精饲粮中添加苦豆子对羔羊生长与血液生化指标的影响

安雅雯^a(), 杨晓东^a, 高智雄^a, 郭绍乾^a, 高爱武^b, 杨金丽^a, 王海荣^a^,^*()

a.内蒙古农业大学动物科学学院, 内蒙古呼和浩特 010018
b.内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特 010018

收稿日期:2021-08-24 出版日期:2022-05-25 发布日期:2022-06-06
通讯作者: 王海荣
作者简介:* 王海荣,E-mail: wanghairong97@163.com
安雅雯(1994—),女,内蒙古锡林郭勒人,博士研究生,主要从事反刍动物饲料研究与开发利用。E-mail: 1070440180@qq.com
基金资助:
国家自然科学基金(31860658)

Effects of adding Sophora alopecuroides to high grain diet on growth and serum biochemical indexes of lambs

AN Yawen^a(), YANG Xiaodong^a, GAO Zhixiong^a, GUO Shaoqian^a, GAO Aiwu^b, YANG Jinli^a, WANG Hairong^a^,^*()

a. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China
b. College of Food Science and Engineering,Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2021-08-24 Online:2022-05-25 Published:2022-06-06
Contact: WANG Hairong

摘要/Abstract

摘要：

为研究高精料中添加苦豆子对育肥羔羊生长性能和血清生化指标的影响,选取32只健康、体重在(25.7±2.17) kg的杜蒙杂交公羔羊,随机分为4组,每组8个重复,试验羔羊单笼饲养,自由饮水和采食。对照组饲喂基础饲粮,T1、T2和T3组在对照组的基础上分别添加0.1%、0.3%和0.5%的苦豆子。试验预试期15 d,正试期60 d。试验结果表明:试验第0~30天,T1组的阶段日增重(SDG)与T3组相比显著升高(P<0.05);第30~60天,T2组的阶段日采食量(SDFI)与对照组相比显著升高(P<0.05)。与对照组相比,T1组谷草转氨酶(AST)和乳酸脱氢酶(LDH)的含量显著升高,而直接胆红素(DBIL)的含量显著降低(P<0.05);第30天时,T2组碱性磷酸酶(ALP)和白蛋白(ALB)的含量显著升高(P<0.05),第60天时,总蛋白(TP)和球蛋白(GLO)的含量显著或极显著升高(P<0.05或P<0.01)。综上所述,在高精饲粮中适量添加苦豆子可促进羔羊的生长,改善羔羊的血液生化状态,且0.1%~0.3%的添加效果较好。

关键词: 苦豆子, 杜蒙羔羊, 生长性能, 血清生化指标

Abstract:

To investigate the effects of adding Sophora alopecuroides to high grain diet on growth performance and serum biochemical indexes of Dumeng lambs, a total of 32 healthy Dumont lambs weighing about (25.7±2.17)kg were randomly divided into 4 groups with 8 replicates in each group. The control group was fed with basal diet, and the T1,T2 and T3 groups were fed with 0.1%,0.3% and 0.5% Sophora alopecuroides, respectively.The pre-test period was 15 days, and the positive test period was 60 days.The results showed as follows: On the 0-30th day of the experiment, the stage daily gain (SDG) of T1 group was significantly (P<0.05) higher than that of T3 group. From 30th to 60th day, the stage daily feed intake (SDFI) of T2 group was significantly (P<0.05) higher than that of control group. Compared with the control group, the concentration of aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in T1 group increased significantly, while the concentration of direct bilirubin (DBIL) decreased significantly (P<0.05). On the 30th day, the contents of alkaline phosphatase (ALP) and albumin (ALB) in T2 group increased significantly (P<0.05), and on the 60th day, the contents of total protein (TP) and globulin (GLO) increased significantly (P<0.05 or P<0.01). It was concluded that adding proper amount of Sophora alopecuroides in high grain diet could promote the growth performance of lambs,improve the blood biochemical state of sheep, and the effect of adding 0.1%-0.3% Sophora alopecuroides was better.

Key words: Sophora alopecuroides, lambs, growth performance, serum biochemistry

中图分类号:

S823.9

安雅雯, 杨晓东, 高智雄, 郭绍乾, 高爱武, 杨金丽, 王海荣. 高精饲粮中添加苦豆子对羔羊生长与血液生化指标的影响[J]. 浙江农业学报, 2022, 34(5): 908-914.

AN Yawen, YANG Xiaodong, GAO Zhixiong, GUO Shaoqian, GAO Aiwu, YANG Jinli, WANG Hairong. Effects of adding Sophora alopecuroides to high grain diet on growth and serum biochemical indexes of lambs[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 908-914.

图/表 6

参考文献 15

[1]	OETZEL G R. Diagnosis and management of subacute ruminal acidosis in dairy herds[J]. The Veterinary Clinics of North America Food Animal Practice, 2017, 33(3): 463-480. DOI URL
[2]	刘艳琴. 苦豆子综合开发利用生产工艺的研究[D]. 西安: 西北大学, 2015.
	LIU Y Q. The production technology research about the comprehensive development and utilization of Sophora alopecuroides[D]. Xi’an: Northwest University, 2015. (in Chinese with English abstract)
[3]	游菁菁, 李月英, 沙碧莹, 等. 中药苦豆子生物碱的研究进展[J]. 江西中医药大学学报, 2015, 27(2): 109-113.
	YOU J J, LI Y Y, SHA B Y, et al. Advances in studies on the alkaloid of Sophora alopecuroides L[J]. Journal of Jiangxi University of Traditional Chinese Medicine, 2015, 27(2): 109-113. (in Chinese with English abstract)
[4]	UR RASHID H, RASOOL S, ALI Y, et al. Anti-cancer potential of sophoridine and its derivatives: recent progress and future perspectives[J]. Bioorganic Chemistry, 2020, 99: 103863. DOI URL
[5]	李海英, 孙国君, 高剑峰, 等. 苦豆子饲喂肉仔鸡效果试验[J]. 石河子大学学报(自然科学版), 2000, 18(2): 172.
	LI H Y, SUN G J, GAO J F, et al. An experiment of effect on feed Sophora alopecuroides L. to broilers[J]. Journal of Shihezi University (Natural Science), 2000, 18(2): 172. (in Chinese)
[6]	王明远, 白婷, 刘小莉, 等. 复方苦豆子对实验性动物血脂和血压的影响[J]. 中国医学创新, 2011, 8(7): 11-13.
	WANG M Y, BAI T, LIU X L, et al. Effects of compound KUDOUZI on lipid and hypertension on the experiment animals[J]. Medical Innovation of China, 2011, 8(7): 11-13. (in Chinese with English abstract)
[7]	谢明欣. 苦豆子对蒙古羔羊体外发酵参数、微生物种群及血液中生理生化指标的影响[D]. 呼和浩特: 内蒙古农业大学, 2018.
	XIE M X. Effect of Sophora alopecuroides L. on rumen fermentation parameters, ruminal microbial population and blood physiological-biochemical indexes of Mongolian lamb[D]. Hohhot: Inner Mongolia Agricultural University, 2018. (in Chinese with English abstract)
[8]	卢福庄, 王志刚, 付媛, 等. 中草药添加剂对猪生产性能和猪瘟疫苗免疫效果的影响[J]. 浙江农业学报, 2011, 23(1): 56-61.
	LU F Z, WANG Z G, FU Y, et al. Effects of Chinese herbal medicine additives on productive performances and levels of antibody against classical swine fever (CSF) for swine[J]. Acta Agriculturae Zhejiangensis, 2011, 23(1): 56-61. (in Chinese with English abstract)
[9]	李昊. 日粮中添加苦豆子对滩羊体外发酵参数、营养物质消化、生长及肉品质影响的研究[D]. 宁夏大学, 2019.
	LI H. Effect of Sophoxa aiopecuroides supplementation in diet on fermentation parameters in vitro,nutrients digestibility, growth and meat quality for tan sheep[D]. Yinchuan: Ningxia University, 2019. (in Chinese with English abstract)
[10]	JIANG Z Y, MENG Y, BO L L, et al. Sophocarpine attenuates LPS-induced liver injury and improves survival of mice through suppressing oxidative stress, inflammation, and apoptosis[J]. Mediators of Inflammation, 2018, 2018: 5871431.
[11]	张会超, 徐里, 芮浩淼, 等. 苦豆碱对缺血再灌注引起的H9c2心肌细胞损伤和炎症应答的作用[J]. 中国病理生理杂志, 2018, 34(2): 281-286.
	ZHANG H C, XU L, RUI H M, et al. Role of aloperine on ischemia-reperfusion-induced injury and inflammatory response in H9c2 cardiomyocytes[J]. Chinese Journal of Pathophysiology, 2018, 34(2): 281-286. (in Chinese with English abstract)
[12]	郭峻菲. 亚急性瘤胃酸中毒(SARA)对反刍动物肝脏的炎性损伤与SARA的调控研究[D]. 南京: 南京农业大学, 2017.
	GUO J F. Inflammatory injury in the liver of ruminants by subacute ruminal acidosis(SARA)and manipulation of SARA[D]. Nanjing: Nanjing Agricultural University, 2017. (in Chinese with English abstract)
[13]	桑秀秀, 王睿林, 韩延忠, 等. 氧化苦参碱通过免疫调节保护刀豆蛋白A诱导的小鼠免疫性肝损伤[J]. 中国实验方剂学杂志, 2017, 23(6): 125-130.
	SANG X X, WANG R L, HAN Y Z, et al. Oxymatrine protects against liver injury induced by con A in mice through suppressing host immune response[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23(6): 125-130. (in Chinese with English abstract)
[14]	王德珍. 黄连生物碱降糖作用及构效关系初探[D]. 重庆: 西南大学, 2014.
	WANG D Z. Preliminary evaluation the antihyperglycemic effect and structure-activity relationship of rhizoma coptidis alkaloids[D]. Chongqing: Southwest University, 2014. (in Chinese with English abstract)
[15]	马建慧, 曹亚军, 陈虹, 等. 复方苦豆子颗粒对高脂血症大鼠血脂代谢的影响[J]. 中国药房, 2007, 18(6): 413-415.
	MA J H, CAO Y J, CHEN H, et al. Effects of compound KuDouZi granules on blood lipid metabolism in hyperlipidemia rats[J]. China Pharmacy, 2007, 18(6): 413-415. (in Chinese with English abstract)

原料Ingredients	含量Content/%	营养水平Nutrient levels	含量Content
混合干草Mixed hay	30.0	代谢能Metabolizable energy/(MJ·kg^-1)	10.4
玉米Corn	53.0	粗蛋白质Crude protein/%	14.4
豆粕Soybean meal	14.9	中性洗涤纤维Neutral detergent fiber/%	23.3
磷酸氢钙CaHPO₄	0.10	酸性洗涤纤维Acid detergent fiber/%	15.0
食盐NaCl	0.50	非结构性碳水化合物Nonstructural carbohydrate/%	49.9
预混料Premix	0.50	钙Ca/%	0.70
小苏打NaHCO₃	1.00	磷P/%	0.30
合计Total	100.0

原料Ingredients	含量Content/%	营养水平Nutrient levels	含量Content
混合干草Mixed hay	30.0	代谢能Metabolizable energy/(MJ·kg^-1)	10.4
玉米Corn	53.0	粗蛋白质Crude protein/%	14.4
豆粕Soybean meal	14.9	中性洗涤纤维Neutral detergent fiber/%	23.3
磷酸氢钙CaHPO₄	0.10	酸性洗涤纤维Acid detergent fiber/%	15.0
食盐NaCl	0.50	非结构性碳水化合物Nonstructural carbohydrate/%	49.9
预混料Premix	0.50	钙Ca/%	0.70
小苏打NaHCO₃	1.00	磷P/%	0.30
合计Total	100.0

组别 Groups	体重 Body weight/kg			阶段日增重 SDG/(g·d^-1)		阶段日采食量 SDFI/(g·d^-1)		全期平均日增重 ADG/(g· d^-1)	全期平均日采食量 ADFI/(g· d^-1)	全期料重比 F/G
组别 Groups	0 d	30 d	60 d	0-30 d	30-60 d	0-30 d	30-60 d	0-60 d	0-60 d	0-60 d
对照组 Control	25.8± 1.18	30.4± 2.41	35.4± 2.84	154.2± 23.9 ab	166.9± 27.6	907.3± 90.6	1108.5± 69.4 b	161.1± 45.9	1007.9± 76.3	6.26± 0.53
group
T1	26.2± 2.70	31.8± 3.92	36.9± 4.53	188.7± 34.5 a	170.9± 44.8	908.1± 91.4	1117.5± 63.4 ab	179.8± 32.8	1012.8± 76.9	5.67± 0.23
T2	25.6± 1.99	30.4± 3.29	35.9± 3.30	158.3± 36.1 ab	187.4± 34.7	919.3± 113.4	1147.3± 70.3 a	172.7± 29.1	1033.3± 91.2	5.96± 0.22
T3	25.7± 2.87	30.2± 2.46	35.4± 3.91	149.4± 22.2 b	175.6± 54.4	900.6± 87.6	1117.5± 63.4 ab	162.5± 28.3	1009.0± 74.5	6.20± 0.24

组别 Groups	体重 Body weight/kg			阶段日增重 SDG/(g·d^-1)		阶段日采食量 SDFI/(g·d^-1)		全期平均日增重 ADG/(g· d^-1)	全期平均日采食量 ADFI/(g· d^-1)	全期料重比 F/G
组别 Groups	0 d	30 d	60 d	0-30 d	30-60 d	0-30 d	30-60 d	0-60 d	0-60 d	0-60 d
对照组 Control	25.8± 1.18	30.4± 2.41	35.4± 2.84	154.2± 23.9 ab	166.9± 27.6	907.3± 90.6	1108.5± 69.4 b	161.1± 45.9	1007.9± 76.3	6.26± 0.53
group
T1	26.2± 2.70	31.8± 3.92	36.9± 4.53	188.7± 34.5 a	170.9± 44.8	908.1± 91.4	1117.5± 63.4 ab	179.8± 32.8	1012.8± 76.9	5.67± 0.23
T2	25.6± 1.99	30.4± 3.29	35.9± 3.30	158.3± 36.1 ab	187.4± 34.7	919.3± 113.4	1147.3± 70.3 a	172.7± 29.1	1033.3± 91.2	5.96± 0.22
T3	25.7± 2.87	30.2± 2.46	35.4± 3.91	149.4± 22.2 b	175.6± 54.4	900.6± 87.6	1117.5± 63.4 ab	162.5± 28.3	1009.0± 74.5	6.20± 0.24

组别 Groups	谷草转氨酶 AST/(U·L^-1)		碱性磷酸酶 ALP/(U·L^-1)		乳酸脱氢酶 LDH/(U·L^-1)		直接胆红素 DBIL/(μmol·L^-1)		总胆红素 TBIL/(μmol·L^-1)		尿素氮 UREA/(μmol·L^-1)
组别 Groups	30 d	60 d	30 d	60 d	30 d	60 d	30 d	60 d	30 d	60 d	30 d	60 d
对照组 Control	86.7± 14.3 B	91.6± 11.9	251.4± 58.3 b	313.6± 60.2	578.1± 76.2 b	473.4± 73.5	0.63± 0.18	1.13± 0.24^** a	10.9± 0.91	9.51± 0.47^**	6.72± 1.29	7.02± 0.41
group
T1	107.7± 14.2 A	100.6± 11.9	254.6± 58.3 b	350.2± 46.1	708.7± 86.9a	418.2± 36.9^**	0.74± 0.21	0.91± 0.19 b	11.4± 0.40	9.32± 0.35^**	6.37± 1.06	6.95± 1.61
T2	71.7± 16.5 B	84.6± 9.4	359.6± 44.9 a	336.7± 43.8	436.3± 68.2 b	469.4± 70.4	0.92± 0.14	0.80± 0.19 b	11.6± 1.40	9.43± 0.67^**	6.26± 0.80	6.87± 1.02
T3	79.5± 10.5 B	110.1± 19.3^**	283.9± 50.2 b	357.9± 53.4	550.5± 65.4 b	472.0± 61.9	0.75± 0.23	1.27± 0.32^** a	11.4± 0.43	9.32± 0.88^**	5.47± 0.90	6.73± 0.74^*

高精饲粮中添加苦豆子对羔羊生长与血液生化指标的影响

Effects of adding Sophora alopecuroides to high grain diet on growth and serum biochemical indexes of lambs

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组别 Groups	总蛋白Total protein		白蛋白Albumin		球蛋白Globulin		白蛋白/球蛋白Albumin/Globulin
组别 Groups	30 d	60 d	30 d	60 d	30 d	60 d	30 d	60 d
对照组 Control	61.3± 7.40	61.8± 4.46 b	30.3± 3.54 b	32.8± 6.18	31.1± 5.43	28.9± 3.95 B	1.00± 0.19	1.17± 0.33
group
T1	60.2± 4.19	67.6± 9.52 ab	31.5± 1.02 b	36.6± 6.97	28.7± 3.54	30.9± 4.29 B	1.11± 0.13	1.19± 0.23
T2	65.7± 4.05	73.8± 2.65^** a	34.9± 4.09 a	35.1± 5.83	30.8± 4.60	38.7± 5.16 A	1.16± 0.23	0.94± 0.28
T3	62.5± 3.11	73.1± 5.38^** a	28.7± 3.25 b	35.2± 4.69^*	33.8± 3.95	37.9± 5.32 A	0.87± 0.17	0.96± 0.26

组别 Groups	总蛋白Total protein		白蛋白Albumin		球蛋白Globulin		白蛋白/球蛋白Albumin/Globulin
组别 Groups	30 d	60 d	30 d	60 d	30 d	60 d	30 d	60 d
对照组 Control	61.3± 7.40	61.8± 4.46 b	30.3± 3.54 b	32.8± 6.18	31.1± 5.43	28.9± 3.95 B	1.00± 0.19	1.17± 0.33
group
T1	60.2± 4.19	67.6± 9.52 ab	31.5± 1.02 b	36.6± 6.97	28.7± 3.54	30.9± 4.29 B	1.11± 0.13	1.19± 0.23
T2	65.7± 4.05	73.8± 2.65^** a	34.9± 4.09 a	35.1± 5.83	30.8± 4.60	38.7± 5.16 A	1.16± 0.23	0.94± 0.28
T3	62.5± 3.11	73.1± 5.38^** a	28.7± 3.25 b	35.2± 4.69^*	33.8± 3.95	37.9± 5.32 A	0.87± 0.17	0.96± 0.26

组别 Groups	血糖Glucose/(mmol·L^-1)		乳酸Lactic acid/(mg·L^-1)
组别 Groups	30 d	60 d	30 d	60 d
对照组Control group	3.81±0.49	4.61±0.46^*	23. 2±5.6	10.1±3.0^**
T1	3.99±0.18	4.99±0.41^**	25.3±3.7	10.8±1.7^**
T2	4.31±0.41	4.67±0.48	18.0±3.8	11.0±3.1^*
T3	3.92±0.46	4.93±0.58^**	18.2±4.9	9.5±1.8^**

组别 Groups	血糖Glucose/(mmol·L^-1)		乳酸Lactic acid/(mg·L^-1)
组别 Groups	30 d	60 d	30 d	60 d
对照组Control group	3.81±0.49	4.61±0.46^*	23. 2±5.6	10.1±3.0^**
T1	3.99±0.18	4.99±0.41^**	25.3±3.7	10.8±1.7^**
T2	4.31±0.41	4.67±0.48	18.0±3.8	11.0±3.1^*
T3	3.92±0.46	4.93±0.58^**	18.2±4.9	9.5±1.8^**