促生长植物激素对桑树叶片衰老过程生理生化指标的影响

doi:10.3969/j.issn.1004-1524.2023.06.05

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (6): 1278-1285.DOI: 10.3969/j.issn.1004-1524.2023.06.05

促生长植物激素对桑树叶片衰老过程生理生化指标的影响

朱燕¹(), 魏佳¹, 许自龙¹, 林天宝¹, 杨升², 刘岩¹, 吕志强¹, 刘培刚¹^,^*()

1.浙江省农业科学院蚕桑与茶叶研究所,浙江杭州 310021
2.浙江省农业科学院亚热带作物研究所,浙江温州 325005

收稿日期:2022-05-05 出版日期:2023-06-25 发布日期:2023-07-04
通讯作者: *刘培刚,E-mail: liupeigang@zaas.ac.cn
作者简介:朱燕(1965—),女,浙江金华人,学士,副研究员,主要从事桑树遗传育种与桑多元化研究。E-mail: zhuy@zaas.ac.cn
基金资助:
浙江省公益技术研究计划(LGN21C160013);“十四五”农业新品种选育重大育种专项蚕桑协作项目(2021C02072-5);现代农业产业技术体系建设专项(CARS-18-270203);温州市基础性农业科技项目(N2020004)

Effects of growth promoting hormones on physiological and biochemical indexes of mulberry leaves during senescence

ZHU Yan¹(), WEI Jia¹, XU Zilong¹, LIN Tianbao¹, YANG Sheng², LIU Yan¹, LYU Zhiqiang¹, LIU Peigang¹^,^*()

1. Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, Zhejiang, China

Received:2022-05-05 Online:2023-06-25 Published:2023-07-04

摘要/Abstract

摘要：

为研究促生长类激素对于桑树叶片衰老过程的影响,以强桑1号(迟衰)和荷叶白(早衰)为研究对象,测定品种间生长素(auxin,IAA)、细胞分裂素(cytokinin,CTK)、赤霉素(gibberellin,GA)含量差异,以及外源激素与抑制剂处理后内源激素含量及抗氧化酶等主要生理生化指标变化情况。结果表明:强桑1号叶片中IAA、CTK和GA含量显著高于同时间节点的荷叶白;同时发现经外源激素处理后3者含量显著增加(P<0.05),抑制剂处理后含量显著(P<0.05)降低。另外,经外源激素处理后,2个品种叶片中过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)及超氧化物歧化酶(superoxide dismutase,SOD) 活性显著提升,可溶性蛋白(soluble protein,SP)和脯氨酸(proline,Pro)含量显著提升,而丙二醛(malondialdehyde,MDA)含量显著降低(P<0.05);经抑制剂处理后,桑树叶片中的CAT、POD、SOD活性,及MDA、SP、Pro含量变化因抑制剂种类和桑树品种不同而存在差异。

关键词: 促生长激素, 桑树, 叶片, 衰老

Abstract:

The content of auxin (IAA), cytokinin (CTK), and gibberellin (GA) and main physiological and biochemical indexes in leaves of Qiangsang 1 (late senescence) and Heyebai (early senescence) before and after application of exogenous hormones and inhibitors were determined in this study for examining the effect of growth-promoting hormones on the senescence of mulberry leaves. The results showed that the IAA, CTK and GA in Qiangsang 1 were significantly higher than those of Heyebai at the same time; the content of them were up-regulated significantly after treatment by exogenous hormones (P<0.05) and down-regulated significantly after treatment by those inhibitor (P<0.05). In addition, after treatment by exogenous hormones, the activities of CAT, POD, and SOD, and SP content and Pro content in leaves were up-regulated significantly, while MDA content was down-regulated significantly (P<0.05). The activities of CAT, POD and SOD, MDA content, SP content and Pro content in mulberry leaves changed differently due to the mulberry varieties and the types of inhibitors after treatment with inhibitors.

Key words: growth promoting hormone, mulberry, leaf, senescence

中图分类号:

S888.2

朱燕, 魏佳, 许自龙, 林天宝, 杨升, 刘岩, 吕志强, 刘培刚. 促生长植物激素对桑树叶片衰老过程生理生化指标的影响[J]. 浙江农业学报, 2023, 35(6): 1278-1285.

ZHU Yan, WEI Jia, XU Zilong, LIN Tianbao, YANG Sheng, LIU Yan, LYU Zhiqiang, LIU Peigang. Effects of growth promoting hormones on physiological and biochemical indexes of mulberry leaves during senescence[J]. Acta Agriculturae Zhejiangensis, 2023, 35(6): 1278-1285.

图/表 5

图1 桑树叶片发育4个不同时期促生长类植物激素的变化 “*”表示与强桑1号中同时期指标相比差异显著(P<0.05)。

Fig.1 Changes in contents of plant growth hormone in mulberry leaves at differential stages “*” represents significant differences at P<0.05.

图2 外源激素及其抑制剂对桑树叶片内源激素水平的影响

Fig.2 Effects of exogenous hormones and their inhibitors on endogenous hormones in mulberry leaves

图3 外源激素及其抑制剂对桑树叶片抗氧化酶活性的影响

Fig.3 Effects of exogenous hormones and their inhibitors on antioxidant enzyme activities in mulberry leaves

图4 外源激素及其抑制剂对桑树叶片MDA 含量的影响

Fig.4 Effects of exogenous hormones and their inhibitors on MDA content in mulberry leaves

图5 外源激素及其抑制剂对桑树叶片 SP 和 Pro 含量的影响

Fig.5 Effects of exogenous hormones and their inhibitors on SP content and Pro content in mulberry leaves

参考文献 17

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促生长植物激素对桑树叶片衰老过程生理生化指标的影响

Effects of growth promoting hormones on physiological and biochemical indexes of mulberry leaves during senescence

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