振动胁迫对蓝莓花色苷代谢及相关基因表达的影响

doi:10.3969/j.issn.1004-1524.20240087

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (3): 622-633.DOI: 10.3969/j.issn.1004-1524.20240087

振动胁迫对蓝莓花色苷代谢及相关基因表达的影响

韩延超¹(), 陈慧芝¹^,², 牛犇¹^,², 张小栓³, 韩树人⁴, 王晓艳¹, 王冠楠¹, 刘瑞玲²^,^*(), 郜海燕¹^,²^,^*()

1.浙江省农业科学院食品科学研究所,全省生鲜食品智慧物流与加工重点实验室,中国轻工业果蔬保鲜与加工重点实验室,浙江杭州 310021
2.农业农村部果品采后处理重点实验室,浙江杭州 310021
3.中国农业大学工学院,北京 100083
4.鲜丰水果股份有限公司,浙江杭州 310000

收稿日期:2024-01-21 出版日期:2024-03-25 发布日期:2024-04-09
作者简介:韩延超(1986—),男,河北衡水人,博士,副研究员,主要从事农产品贮藏与加工研究。E-mail: hanhanzhixing@126.com
通讯作者: *刘瑞玲,E-mail: ruilingliu2005@163.com;郜海燕,E-mail: spsghy@163.com
基金资助:
浙江省“尖兵”“领雁”研发攻关计划(2023C2006);浙江省“尖兵”“领雁”研发攻关计划(2023C04002);国家重点研发计划(2021YFD2100502-1-2)

Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry

HAN Yanchao¹(), CHEN Huizhi¹^,², NIU Ben¹^,², ZHANG Xiaoshuan³, HAN Shuren⁴, WANG Xiaoyan¹, WANG Guannan¹, LIU Ruiling²^,^*(), GAO Haiyan¹^,²^,^*()

1. Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Key Laboratory of Fruit and Vegetable Preservation and Processing in China’s Light Industry, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Key Laboratory of Fruit Postharvest Treatment of the Ministry of Agriculture and Rural Affairs, Hangzhou 310021, China
3. College of Engineering, China Agricultural University, Beijing 100083, China
4. Xianfeng Fruit Co., Ltd., Hangzhou 310000, China

Received:2024-01-21 Online:2024-03-25 Published:2024-04-09

摘要/Abstract

摘要：

蓝莓果实富含花色苷等生物活性物质,营养价值高。但物流振动损伤会加速蓝莓花色苷等营养物质损耗,降低营养品质。文章以蓝美人品种蓝莓为实验材料,通过模拟物流振动,研究了振动胁迫对蓝莓花色苷组分、代谢相关酶活性以及基因表达的影响。研究结果显示,蓝莓果实中含有10种花色苷单体,其中锦葵素-3-阿拉伯糖苷含量最高。在蓝莓贮藏前期,振动胁迫可加速花色苷积累,显著提高苯丙氨酸解氨酶(PAL)和类黄酮糖苷转移酶(UFGT)活性,诱导花色苷合成相关基因VcPAL1、VcDFR2、VcCHI1、VcUFGT等表达。而贮藏后期,振动胁迫组的PAL、查尔酮异构酶(CHI)、二氢黄酮醇还原酶(DFR)、UFGT活性均显著低于对照组,同时,振动胁迫还可延缓过氧化物酶(POD)、多酚氧化酶(PPO)和花色苷-β-糖苷酶等花色苷降解相关酶活性的下降,抑制花色苷合成相关基因表达,促进VcPOD1、VcPOD2、VcPOD3和VcPPO1等花色苷降解相关基因表达。综上,振动胁迫通过提高花色苷合成酶活性以及相关酶基因的表达,加速蓝莓贮藏前期花色苷的积累;而贮藏后期,振动胁迫则通过延缓花色苷降解酶活性下降以及相关酶基因表达,促进花色苷的降解。研究结果为调控蓝莓花色苷代谢提供了理论依据。

关键词: 蓝莓, 花色苷, 酶, 基因

Abstract:

Blueberry is rich in bioactive substances such as anthocyanin and has high nutritional value. However, logistics vibration damage can accelerate the loss of nutrients such as blueberry anthocyanin and reduce nutritional quality. This article took the blueberry variety Lanmeiren as the experimental material and studied the effect of vibration stress on the anthocyanin components, metabolic enzymes activities, and genes expression of blueberry by simulating logistics vibration. The research results showed that blueberry contained 10 types of anthocyanin monomers, among which the content of quercetin-3-arabinose was the highest. In the early stage of blueberry storage, vibration stress accelerated the accumulation of anthocyanin, significantly increased the activities of phenylalanine ammonia lyase (PAL) and flavonoid glycosyltransferase (UFGT), and induced the expression of genes related to anthocyanin synthesis, such as VcPAL1, VcDFR2, VcCHI1, and VcUFGT. In the later stage of storage, the activities of PAL, chalcone isomerase (CHI), dihydroflavonol reductase (DFR), and UFGT in the vibration stress group were significantly lower than those in the control group. Meanwhile, vibration stress also delayed the decrease in the activity of enzymes related to anthocyanin degradation, such as peroxidase (POD), polyphenol oxidase (PPO) and anthocyanin-β-glucosidase, inhibited the expression of genes related to anthocyanin synthesis, and promoted the expression of genes related to anthocyanin degradation, such as VcPOD1, VcPOD2, VcPOD3, and VcPPO1. In summary, vibration stress accelerated the accumulation of anthocyanin in blueberry during early storage by increasing the activity of anthocyanin synthase and the expression of related enzyme genes. In the later stage of storage, vibration stress promoted the degradation of anthocyanin by delaying the decrease of anthocyanin degrading enzymes activities and related enzyme genes expression. The research results provided a theoretical basis for regulating the metabolism of blueberry anthocyanin.

Key words: blueberry, anthocyanin, enzyme, gene

中图分类号:

韩延超, 陈慧芝, 牛犇, 张小栓, 韩树人, 王晓艳, 王冠楠, 刘瑞玲, 郜海燕. 振动胁迫对蓝莓花色苷代谢及相关基因表达的影响[J]. 浙江农业学报, 2024, 36(3): 622-633.

HAN Yanchao, CHEN Huizhi, NIU Ben, ZHANG Xiaoshuan, HAN Shuren, WANG Xiaoyan, WANG Guannan, LIU Ruiling, GAO Haiyan. Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry[J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 622-633.

图/表 7

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引物名称 Primer name	上游引物序列 Sequence of forward primer(5'-3')	下游引物序列 Sequence of reverse primer(5'-3')
VcPAL1	TCATGTCCAAAGTGCTGAGC	AACCAAGTGGCACTCATGAG
VcPAL2	GTTCGCATCAACACCCTCCT	GGCCCTACCGCTTTTGAGTT
VcCHI1	CAGGCAACTCCATTCTTTTC	TTCTCTATGACTGCATTCCC
VcCHI2	GCCCTTATTTCTGCTCCAGTTG	CTCTAGCTGCACACCGTACT
VcDFR1	AACCTAACGCTGTGGAAGGC	ATACTCCGACGCAACCTTCA
VcDFR2	AGAAAGCAGCATGGGAAGCA	GCTTGGTGGGAATGTAGGCA
VcDFR3	CGAGCAACCGTTCGCGATCCA	AGGTCCGCCTTCCACAGCGT
VcUFGT	AGTTTGCTTTGAAGGCTGTTG	ATGTGCTGGTGTGCATTTG
VcPOD1	ACGTTGCTTCAAAATGTGGCTT	TCCTTGAGTTTTGTACTTCTCGTAG
VcPOD2	TGCTGGTGTTGTTGCAGTTG	CGCCCTTCCTTGGGAGAAAT
VcPOD3	CTGGAGCCCATCAAGGAACA	TCCATGGGACTCTGGATGGA
VcPPO1	GCCGACTTTTAAGCCACGGA	GCTTGTCAGGGTGAAGGTGA
VcPPO2	GAGATCCTCCAACGACTCACA	AGCAGGTTTCAGTGCCCAA
VcGAPDH	ACTACCATCCACTCTATCACCG	AACACCTTACCAACAGCCTTG

引物名称 Primer name	上游引物序列 Sequence of forward primer(5'-3')	下游引物序列 Sequence of reverse primer(5'-3')
VcPAL1	TCATGTCCAAAGTGCTGAGC	AACCAAGTGGCACTCATGAG
VcPAL2	GTTCGCATCAACACCCTCCT	GGCCCTACCGCTTTTGAGTT
VcCHI1	CAGGCAACTCCATTCTTTTC	TTCTCTATGACTGCATTCCC
VcCHI2	GCCCTTATTTCTGCTCCAGTTG	CTCTAGCTGCACACCGTACT
VcDFR1	AACCTAACGCTGTGGAAGGC	ATACTCCGACGCAACCTTCA
VcDFR2	AGAAAGCAGCATGGGAAGCA	GCTTGGTGGGAATGTAGGCA
VcDFR3	CGAGCAACCGTTCGCGATCCA	AGGTCCGCCTTCCACAGCGT
VcUFGT	AGTTTGCTTTGAAGGCTGTTG	ATGTGCTGGTGTGCATTTG
VcPOD1	ACGTTGCTTCAAAATGTGGCTT	TCCTTGAGTTTTGTACTTCTCGTAG
VcPOD2	TGCTGGTGTTGTTGCAGTTG	CGCCCTTCCTTGGGAGAAAT
VcPOD3	CTGGAGCCCATCAAGGAACA	TCCATGGGACTCTGGATGGA
VcPPO1	GCCGACTTTTAAGCCACGGA	GCTTGTCAGGGTGAAGGTGA
VcPPO2	GAGATCCTCCAACGACTCACA	AGCAGGTTTCAGTGCCCAA
VcGAPDH	ACTACCATCCACTCTATCACCG	AACACCTTACCAACAGCCTTG

花色苷组分 Anthocyanin components	0 d		4 d		8 d		12 d		16 d		20 d		24 d
花色苷组分 Anthocyanin components	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h
飞燕草素-3-半乳糖苷 Delphinidin-3-galactoside	644.68 ±6.78	754.65 ±21.41^*	649.76 ±22.09	827.79 ±9.08^*	646.84 ±38.42	881.23 ±38.42^*	721.09 ±2.70	717.34 ±18.55	909 ±9.63	562.41 ±20.76^*	919.85 ±22.46	473.84 ±17.55^*	508.62 ±38.78	303.85 ±32.33^*
矢车菊素-3-半乳糖苷 Cyanin-3-galactoside	362.81 ±15.10	585.98 ±22.22^*	372.35 ±11.89	649.50 ±15.58^*	380.73 ±18.30	693.26 ±15.22^*	391.67 ±12.80	571.28 ±25.91^*	699.28 ±5.80	416.17 ±12.63^*	494.29 ±23.58	304.06 ±7.57^*	188.51 ±18.78	122.16 ±2.96^*
飞燕草素-3-阿拉伯糖苷 Delphinidin-3-arabinose glycoside	376.21 ±4.53	650.37 ±10.29^*	410.91 ±14.91	517.54 ±19.38^*	456.91 ±9.40	539.39 ±8.58^*	569.62 ±15.54	473.08 ±13.22^*	612.00 ±13.64	370.01 ±19.06^*	647.95 ±25.27	258.88 ±13.46^*	313.97 ±7.50	119.43 ±18.55^*
矮牵牛素-3-半乳糖苷 Petunionin-3-galactoside	41.35 ±2.21	47.58 ±1.49^*	46.95 ±4.58	54.51 ±2.52^*	52.01 ±2.25	68.03 ±2.32^*	58.53 ±2.69	42.68 ±4.19^*	67.00 ±5.85	46.52 ±1.62^*	51.29 ±5.42	40.52 ±1.69^*	41.17 ±1.19	29.41 ±1.41^*
矮牵牛素-3-葡萄糖苷 Petunionin-3-glucoside	67.11 ±4.66	82.82 ±12.23	71.22 ±6.19	92.63 ±11.53^*	75.35 ±6.53	141.45 ±4.44^*	116.59 ±9.60	79.17 ±9.75^*	129.36 ±5.51	64.73 ±5.10^*	100.67 ±8.54	49.39 ±6.57^*	91.01 ±9.45	35.66 ±7.12^*
芍药素-3-葡萄糖苷 Paeoniflorin-3-glucoside	7.95 ±1.50	14.99 ±1.26^*	9.10 ±1.42	19.93 ±1.48^*	8.36 ±3.78	25.66 ±0.64^*	10.68 ±1.13	17.73 ±1.22^*	12.58 ±1.73	9.76 ±0.86^*	18.75 ±2.20	5.51 ±0.64^*	22.25 ±12.29	1.78 ±0.38^*
矮牵牛素-3-阿拉伯糖苷 Petunionin-3-arabinose glycoside	62.76 ±8.64	97.03 ±8.33^*	107.17 ±8.73	102.73 ±2.02	119.68 ±4.61	135.12 ±10.81	144.28 ±2.73	97.43 ±3.81^*	99.20 ±5.61	67.28 ±3.84^*	55.45 ±6.33	38.42 ±6.48^*	37.96 ±5.71	21.56 ±3.01^*
锦葵素-3-半乳糖苷 Malvacin-3-galactoside	817 ±27.81	962.59 ±34.18^*	851.76 ±11.51	856.38 ±26.93^*	912.44 ±14.96	774.60 ±24.83^*	932.58 ±16.85	599.27 ±6.50^*	714.22 ±79.10	534.08 ±34.81	534.53 ±23.99	365.28 ±27.98^*	414.20 ±16.71	189.87 ±24.71^*
锦葵素-3-葡萄糖苷 Malvacin-3-glucoside	36.26 ±2.54	39.03 ±2.02	37.26 ±1.28	38.27 ±0.95	41.26 ±1.81	44.55 ±2.50	41.44 ±0.80	35.36 ±1.38^*	36.83 ±2.40	33.26 ±1.75	46.79 ±1.59	37.40 ±2.69^*	31.97 ±1.55	28.06 ±2.61^*
锦葵素-3-阿拉伯糖苷 Malvacin-3-arabinose glycoside	5 246.72 ±503.30	6 166.53 ±179.92^*	5 289.86 ±220.22	7 276.47 ±286.78^*	6 930.70 ±142.71	10 746.69 ±133.75^*	8 313.87 ±377.03	7 740.23 ±291.46^*	8 719.88 ±365.39	5 296.44 ±22.67^*	6 282.75 ±75.21	3 788.40 ±358.62^*	2 717.32 ±128.54	2 117.73 ±251.78^*

花色苷组分 Anthocyanin components	0 d		4 d		8 d		12 d		16 d		20 d		24 d
花色苷组分 Anthocyanin components	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h
飞燕草素-3-半乳糖苷 Delphinidin-3-galactoside	644.68 ±6.78	754.65 ±21.41^*	649.76 ±22.09	827.79 ±9.08^*	646.84 ±38.42	881.23 ±38.42^*	721.09 ±2.70	717.34 ±18.55	909 ±9.63	562.41 ±20.76^*	919.85 ±22.46	473.84 ±17.55^*	508.62 ±38.78	303.85 ±32.33^*
矢车菊素-3-半乳糖苷 Cyanin-3-galactoside	362.81 ±15.10	585.98 ±22.22^*	372.35 ±11.89	649.50 ±15.58^*	380.73 ±18.30	693.26 ±15.22^*	391.67 ±12.80	571.28 ±25.91^*	699.28 ±5.80	416.17 ±12.63^*	494.29 ±23.58	304.06 ±7.57^*	188.51 ±18.78	122.16 ±2.96^*
飞燕草素-3-阿拉伯糖苷 Delphinidin-3-arabinose glycoside	376.21 ±4.53	650.37 ±10.29^*	410.91 ±14.91	517.54 ±19.38^*	456.91 ±9.40	539.39 ±8.58^*	569.62 ±15.54	473.08 ±13.22^*	612.00 ±13.64	370.01 ±19.06^*	647.95 ±25.27	258.88 ±13.46^*	313.97 ±7.50	119.43 ±18.55^*
矮牵牛素-3-半乳糖苷 Petunionin-3-galactoside	41.35 ±2.21	47.58 ±1.49^*	46.95 ±4.58	54.51 ±2.52^*	52.01 ±2.25	68.03 ±2.32^*	58.53 ±2.69	42.68 ±4.19^*	67.00 ±5.85	46.52 ±1.62^*	51.29 ±5.42	40.52 ±1.69^*	41.17 ±1.19	29.41 ±1.41^*
矮牵牛素-3-葡萄糖苷 Petunionin-3-glucoside	67.11 ±4.66	82.82 ±12.23	71.22 ±6.19	92.63 ±11.53^*	75.35 ±6.53	141.45 ±4.44^*	116.59 ±9.60	79.17 ±9.75^*	129.36 ±5.51	64.73 ±5.10^*	100.67 ±8.54	49.39 ±6.57^*	91.01 ±9.45	35.66 ±7.12^*
芍药素-3-葡萄糖苷 Paeoniflorin-3-glucoside	7.95 ±1.50	14.99 ±1.26^*	9.10 ±1.42	19.93 ±1.48^*	8.36 ±3.78	25.66 ±0.64^*	10.68 ±1.13	17.73 ±1.22^*	12.58 ±1.73	9.76 ±0.86^*	18.75 ±2.20	5.51 ±0.64^*	22.25 ±12.29	1.78 ±0.38^*
矮牵牛素-3-阿拉伯糖苷 Petunionin-3-arabinose glycoside	62.76 ±8.64	97.03 ±8.33^*	107.17 ±8.73	102.73 ±2.02	119.68 ±4.61	135.12 ±10.81	144.28 ±2.73	97.43 ±3.81^*	99.20 ±5.61	67.28 ±3.84^*	55.45 ±6.33	38.42 ±6.48^*	37.96 ±5.71	21.56 ±3.01^*
锦葵素-3-半乳糖苷 Malvacin-3-galactoside	817 ±27.81	962.59 ±34.18^*	851.76 ±11.51	856.38 ±26.93^*	912.44 ±14.96	774.60 ±24.83^*	932.58 ±16.85	599.27 ±6.50^*	714.22 ±79.10	534.08 ±34.81	534.53 ±23.99	365.28 ±27.98^*	414.20 ±16.71	189.87 ±24.71^*
锦葵素-3-葡萄糖苷 Malvacin-3-glucoside	36.26 ±2.54	39.03 ±2.02	37.26 ±1.28	38.27 ±0.95	41.26 ±1.81	44.55 ±2.50	41.44 ±0.80	35.36 ±1.38^*	36.83 ±2.40	33.26 ±1.75	46.79 ±1.59	37.40 ±2.69^*	31.97 ±1.55	28.06 ±2.61^*
锦葵素-3-阿拉伯糖苷 Malvacin-3-arabinose glycoside	5 246.72 ±503.30	6 166.53 ±179.92^*	5 289.86 ±220.22	7 276.47 ±286.78^*	6 930.70 ±142.71	10 746.69 ±133.75^*	8 313.87 ±377.03	7 740.23 ±291.46^*	8 719.88 ±365.39	5 296.44 ±22.67^*	6 282.75 ±75.21	3 788.40 ±358.62^*	2 717.32 ±128.54	2 117.73 ±251.78^*

振动胁迫对蓝莓花色苷代谢及相关基因表达的影响

Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry

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