›› 2012, Vol. 24 ›› Issue (6): 0-1020.

• 论文 •    

菜用大豆籽粒发育过程中蔗糖积累及相关酶活性的研究

张古文,胡齐赞,徐盛春,龚亚明*   

  1. 浙江省农业科学院 蔬菜研究所,浙江 杭州 310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-11-25 发布日期:2012-11-25

Study on sucrose accumulation and enzyme activities involved in sucrose metabolism in developing seeds of vegetable soybean

ZHANG Guwen;HU Qizan;XYU Shengchun;GONG Yaming*   

  1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-11-25 Published:2012-11-25

摘要: 选用蔗糖含量差异显著的菜用大豆品种——‘浙农6号’(蔗糖含量高)、‘辽鲜1号’(蔗糖含量中等)和‘夏丰2008’(蔗糖含量低),对三者籽粒发育过程中蔗糖、可溶性总糖含量以及蔗糖合成酶(SS)、酸性转化酶(AI)、中性转化酶(NI)、蔗糖磷酸合成酶(SPS)等蔗糖代谢相关酶活性进行了比较,并对蔗糖积累与酶活性的关系进行了分析。结果表明,菜用大豆籽粒干、鲜重积累均呈单“S”型曲线,三个品种的蔗糖代谢规律基本相似,整个菜用大豆籽粒发育过程中,蔗糖是可溶性总糖的主要成分,约占其总含量的70%。蔗糖代谢相关酶的净活性是影响蔗糖积累的主要因子,在籽粒发育早期,蔗糖代谢相关酶的净活性为负值,蔗糖以分解为主,此时蔗糖合成酶和酸性转化酶是催化其分解的关键酶;籽粒发育中期,蔗糖代谢相关酶的净活性转为正值,蔗糖代谢转为合成为主,蔗糖合成酶和蔗糖磷酸合成酶是催化其合成的关键酶;进入籽粒发育后期,蔗糖代谢相关酶的净活性仍为正值,但接近于零,表明蔗糖的合成与分解速率基本相当,此时蔗糖合成酶、酸性转化酶、中性转化酶和蔗糖磷酸合成酶在蔗糖代谢中起主导作用。

关键词: 菜用大豆, 籽粒发育, 蔗糖代谢, 酶活性

Abstract: Three cultivars, Zhenong No.6 (high sucrose content), Liaoxian No.1 (middle sucrose content) and Xiafeng 2008 (low sucrose content) were used to investigate the sucrose accumulation and enzyme activities involved in its metabolism in developing seeds of vegetable soybean. The results showed that the growth curves of vegetable soybean seeds showed single sigmoid pattern. The change trends of sucrose metabolism among three cultivars were similar. During the whole periods of vegetable soybean seeds development, sucrose was the major component of soluble sugar, taking about 70% of total soluble sugar contents. The net activities of sucrose\|metabolizing enzymes were the major factor affecting sucrose accumulation. During the early stage of seed development, net activities of sucrose\|metabolizing enzymes were negative value. Sucrose metabolism is in the decomposition direction, when sucrose synthase and acid invertase were key enzymes in sucrose decomposition. However, the net activities of sucrose\|metabolizing enzymes turned to positive value in the middle stage of seed development. Sucrose metabolism was in the synthesis direction, when sucrose synthase and sucrose phosphate synthase were key enzymes in sucrose synthesis. During the late stage of seed development, also the net activities of sucrose\|metabolizing enzymes were positive value, but its value was close to zero, showing that the rate of sucrose decomposition and synthesis was similar and sucrose synthase, sucrose phosphate synthase, acid invertase and neutral invertase played a leading role in sucrose metabolism in this stage.

Key words: vegetable soybean, seed development, sucrose metabolism, net activity