浙江农业学报

• 作物科学 • 上一篇    下一篇

浙江省46个粳稻新品种SSR指纹图谱构建及遗传多样性分析

  

  1. (1 杭州师范大学 生命与环境科学学院,浙江 杭州 310036;2 浙江省农业科学院 作物与核技术利用研究所,浙江 杭州 310021;3 浙江师范大学 化学与生命科学学院,浙江 金华 321004; 4 嘉兴市农业科学研究院,浙江 嘉兴 314016)
  • 出版日期:2014-09-25 发布日期:2014-10-11

Fingerprint construction and analysis of genetic diversity with SSR markers for 46 japonica rice varieties in Zhejiang Province

  1. (1 College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China; 4 Jiaxing Acaclemy of Agricultural Sciences, Jiaxing 314016, China)
  • Online:2014-09-25 Published:2014-10-11

摘要: 采用农业部推荐的12对水稻SSR引物(NY/T 1433—2007),对2008年以来由浙江省主要育种单位育成的46个常规粳稻品种构建DNA指纹图谱,并分析其亲缘关系。结果显示,12对SSR引物共检测出84个等位基因,平均多态性频率(FP)值为0192。46个品种的相似系数在0735~1000之间,平均相似系数为0879,表明近年来浙江省粳稻品种亲缘较近,遗传背景较狭窄。该研究结果可为浙江省水稻新品种选育、鉴别和保护提供参考。

关键词: 常规粳稻, 指纹图谱, SSR标记, 遗传多样性

Abstract: In order to provide references for rice breeding in Zhejiang Province, 12 pairs of rice SSR primers recommended by Ministry of Agriculture (NY/T 1433—2007) were adopted in the present research to construct DNA fingerprints for 46 japonica rice varieties bred in Zhejiang Province since 2008, as well as to analyze their genetic relationship. It was shown that 84 alleles were detected by 12 pairs of SSR markers, and the average frequency of polymorphism (FP) was 0192. The similarity coefficient of 46 cultivars was between 0735-1000 with an average of 0879, which indicated a close affinity among the tested cultivars and a narrow breeding background in Zhejiang Province.

Key words: conventional japonica rice, fingerprint, SSR marker, genetic diversity