浙江农业学报

• 动物科学 • 上一篇    下一篇

微卫星标记技术鉴定某保种场四川白鹅保种效果

  

  1. (1浙江省农业科学院 畜牧兽医研究所,浙江 杭州310021;2南京农业大学 动物科技学院,江苏 南京210095)
  • 出版日期:2015-05-25 发布日期:2015-05-19

Conservation efficiency of Sichuan white goose in a conservation farm analyzed by microsatellite markers

  1. (1 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2 College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China)
  • Online:2015-05-25 Published:2015-05-19

摘要: 运用微卫星标记技术对某保种场采用小群体保种法保种的四川白鹅3个世代进行了遗传多样性分析,并对3个世代间的遗传多样性进行差异比较;同时对3个世代进行哈代温伯格平衡检验,遗传距离及相似度分析。结果表明:3个世代在所选取的7个微卫星位点上的平均多态信息含量分别为0474 1,0486 6,0471 8,平均期望杂合度分别为0538 7,0551 1,0529 5,这3个群体间在优势等位基因、多态信息含量(PIC)、期望杂合度(He)上差异不显著;3个世代间相似性最小为0970 4,最大遗传距离为0030 1,表明保种群体的遗传结构没有分化,保种效果良好。但经哈代温伯格平衡检验表明,群体的遗传结构处于一个逐渐不平衡的状态。

关键词: 四川白鹅, 微卫星标记, 保种, 遗传多样性

Abstract: Microsatellite marker was used to analyze the differences of genetic diversity among three generations of Sichuan white gooses, which were protected in small group conservation. Meantime, Hardy\|Weinberg equilibrium test, genetic distance and similarity analysis among the three generations were conducted as well. The results indicated: the average polymorphism information content on selected seven microsatellite sites in the three generations were 04741, 04866, 04718; Means of expected heterozygosity were 05387, 05511, 05295, respectively. There were no significant differences in advantage alleles, polymorphic information content(PIC) and expected heterozygosity (He) among three generations. The minimum of genetic similarity in three generations was 09704, maximum of genetic distance was 00301. This indicated that there were a good reservation and no differentiations in genetic constitution in small group. But by the Hardy\|Weinberg equilibrium test, it showed that the genetic structure of the group was in an unbalanced state gradually.

Key words: Sichuan white goose, microsatellite markers, conservation, genetic diversity